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Kloakk
Strange

Registered: 10/21/24
Posts: 6,094
Loc: Scandinavia
Last seen: 22 minutes, 57 seconds

Re: AGAR ENVY! (Anything and All things agar!)

[Re: BeefExtreme]
#29350733 - 09/21/25 11:52 AM (1 year, 24 days ago)

BeefSupremeJr said:

dude really good. cant understand a word but i like the vibes.

not sure if this will work but heres a little project i did a while back

edit: or try this maybe..

That was a nice little sound trip you've made, I liked it :hatsoff:

:hatsoff:

And thanks, that track is actually pretty far away from how I did rap music, but it was just one of those beats I felt compelled to write lyrics to :muppet:

:muppet:

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Some fungi are only edible once.”
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philmyco
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Mackload]
#29350896 - 09/21/25 02:32 PM (1 year, 24 days ago)

Mackload said:

philmyco said:

Relatively new to agar. Here is some pan cyan - does this look bacterial/contaminated? Seems like it isn’t a clean plate

bit hard to tell with the color but looks ok to me. maybe ask in the pans thread

Will do, my other plates don’t have the little brown splotches. I think I’ll avoid the pink plates in the future , they are a bit hard to read compared to the boring light brown ones

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Proposition 122
Bonehead

Registered: 11/14/22
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Loc: Colorado

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Re: AGAR ENVY! (Anything and All things agar!)

[Re: SpaceDawg]

1
#29350934 - 09/21/25 03:08 PM (1 year, 24 days ago)

SpaceDawg said:

Proposition 122 said:

Nice shots SD! Are those glass plates?

Thanks! And the plates are Vabiooth autoclavable plastic plates from the 'zon. Lots of people seem to use them around here. I'm liking them a lot so far.

Check them out

Thanks for the link :heart:

:heart:

I'm going to try them.

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philmyco
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Proposition 122]
#29351029 - 09/21/25 05:17 PM (1 year, 23 days ago)

I saw a YouTube video of a guy using the water from a grain boil to mix with agar to make plates, is that a pretty normal practice? Seems to work very well for him

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RoscoeReturns
We’ve outstayed our welcome

Registered: 02/13/18
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: philmyco]
#29351061 - 09/21/25 05:54 PM (1 year, 23 days ago)

philmyco said:

I saw a YouTube video of a guy using the water from a grain boil to mix with agar to make plates, is that a pretty normal practice? Seems to work very well for him

Grain water agar is a thing, and lots of folks use it.

I have two problems with it. You never know how nutritious it is, and there is a possibility for toxins/antifungals that some grain is treated with to build up in your grain water and end up in your agar. MEA is so easy and cheap I can’t justify using grain water. I do pour it on my compost pile.

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cronicr

Registered: 08/07/11
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: RoscoeReturns]

2
#29351084 - 09/21/25 06:19 PM (1 year, 23 days ago)

:whathesaid:

I won't use grain water agar, a bag of brf is 4 bucks

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tryptkaloids
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: cronicr]
#29351087 - 09/21/25 06:22 PM (1 year, 23 days ago)

I wanted to know just how nutritious grain water is so i made grain as normal, measuring out just enough water to hydrate a brick of coir, sterilized some coir hydrated with it.

I then injected 20ml of the same lc into 4 seperate bags. 2 bags were 3 lbs straight grain water hydrated coir, 2 bags were 2lb coir 1lb hydrated grain.

No cube myc recovered on either grain water bag. As the aio bags were finishing up i checked the other ones, small mold colony on one from an improper bag seal, but otherwise nothing to note.

Hardly a conclusive sample size, and im sure theres something in there to be utilized, but im still not sure how useful it really is.

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
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Arkyhog
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: RoscoeReturns]

9
#29351152 - 09/21/25 07:59 PM (1 year, 23 days ago)

RoscoeReturns said:

philmyco said:

I saw a YouTube video of a guy using the water from a grain boil to mix with agar to make plates, is that a pretty normal practice? Seems to work very well for him

Grain water agar is a thing, and lots of folks use it.

I have two problems with it. You never know how nutritious it is, and there is a possibility for toxins/antifungals that some grain is treated with to build up in your grain water and end up in your agar. MEA is so easy and cheap I can’t justify using grain water. I do pour it on my compost pile.

My wife takes every bit of grain water I have after hydrating my oats for her house plants. They love it!

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tree frog
sapphic love muffin

Registered: 03/31/14
Posts: 6,991

Re: AGAR ENVY! (Anything and All things agar!)

[Re: philmyco]
#29351419 - 09/22/25 01:29 AM (1 year, 23 days ago)

philmyco said:

Relatively new to agar. Here is some pan cyan - does this look bacterial/contaminated? Seems like it isn’t a clean plate

I don't like the scalloping.

If you have other transfers I would skip this one. Otherwise run it out on water agar or through a cabin.

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Xegarus
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Registered: 08/31/25
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tormato]
#29351498 - 09/22/25 06:16 AM (1 year, 23 days ago)

Hey, first time using agar.

I added a drop or two from a spore syringe and this is what i have now. I only have 4 more agar plates at the moment and was wondering what my best option would be to clean this up?

Shoukd i take multiple samples for my second generation or since im a bit limited on plates, just take one and try to get another 2 or 3 generation from that?

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AZ Starman2112
Male Half of a Friendly Couple

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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Xegarus]

3
#29351641 - 09/22/25 10:56 AM (1 year, 23 days ago)

Sorrr for the condensation, but I assure you they are really pretty!

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BeefExtreme
Meatkeeper

Registered: 11/03/11
Posts: 14,061
Loc:

Last seen: 6 minutes, 54 seconds

Re: AGAR ENVY! (Anything and All things agar!)

[Re: AZ Starman2112]

1
#29351647 - 09/22/25 11:08 AM (1 year, 23 days ago)

taking pics of plates is a whole artform. i wish some real photographer would write a tek sometime.

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AZ Starman2112
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: BeefExtreme]
#29351650 - 09/22/25 11:11 AM (1 year, 23 days ago)

BeefSupremeJr said:

taking pics of plates is a whole artform. i wish some real photographer would write a tek sometime.

You are correct. And getting them with a phone is quick & fast. Maybe one day in the future I’ll experiment with my real camera.

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BeefExtreme
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: AZ Starman2112]

1
#29351744 - 09/22/25 12:52 PM (1 year, 23 days ago)

if you use manual focus sometimes you can get it to focus on the culture instead of the lid but its real fuckin hard.

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tryptkaloids
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Posts: 16,534
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: BeefExtreme]

1
#29351789 - 09/22/25 01:22 PM (1 year, 23 days ago)

Just open em and picture in the flow

--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
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sloug
Strangler

Registered: 02/07/22
Posts: 554
Last seen: 3 days, 16 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: tryptkaloids]

6
#29352031 - 09/22/25 04:43 PM (1 year, 22 days ago)

natural backlit works pretty good for me

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Gilmour Hendrix
Village Idiot

Registered: 08/02/25
Posts: 1,689
Loc: Bacta-Mold HQ

Re: AGAR ENVY! (Anything and All things agar!)

[Re: sloug]

5
#29352640 - 09/23/25 05:00 AM (1 year, 22 days ago)

I'm using a floor lamp for my back light. Thought this Pan myc was pretty cool to be growing out of the roof of the cabin.

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Azule
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Registered: 09/10/25
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Re: AGAR ENVY! (Anything and All things agar!)

[Re: Gilmour Hendrix]
#29352700 - 09/23/25 08:17 AM (1 year, 22 days ago)

I'm testing LC of psilocybe azurescens that I bought online. 4 out of 6 dishes looks promising, can you please help me with deciding what should I transfer further? The plates were inoculated 13 days ago.

Plate 1 has malt (not malt extract) agar and mycelium looks very thick:

Is such a thick mycelium a good sign? Or rather a sign of some contamination and mycelium gets thick to fight it?

The plates 2,3,4 are grain oat water, with a little of activated carbon (200mg to 500ml), added mostly for colouring. I can see there some contamination (different colour). Are all the other white things mycelium?

On plate 3, 2 colonies joined together, that's why it's uneven.

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Tropical
Here

Registered: 07/21/24
Posts: 821

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Azule]
#29353270 - 09/23/25 05:48 PM (1 year, 21 days ago)

Hello,

A question about storing plates.

I've done some pour 12h ago, i've let them in the SAB during this time (did not flip those had to go).

1. How long should I leave those in the SAB ? Can I remove those immediatly after pouring ?

2. If I don't plan to use those, do I wrap them ? If yes, how many hours after the pour ? When no condensation anymore ?

3. If condensation dropplets fell on the agar, is it bothersome ?

Ty

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cronicr

Registered: 08/07/11
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Loc: Van Isle

Last seen: 3 days, 4 hours

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Tropical]
#29353404 - 09/23/25 07:44 PM (1 year, 21 days ago)

Let them.cool until the agar has gelled , couple hours is more than enough, wrap them until you're ready

--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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