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Re: AGAR ENVY! (Anything and All things agar!) [Re: anders] * 1
    #29315228 -

anders said:
Wouldn't sterilization matter?

If not I am happy to forget it.


If you were to colonize it sealed like in a spawn bag or a jar. And inoculate it in a sab or flowhood, then it could work.

If you are sterilizing it, then dumping it on coir open air it will mold before it gets colonized


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #29315239 -

I see.  Ok so much for that idea then.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Centerfinger] * 2
    #29316127 -

Centerfinger said:
Does this transfer look isolated? This is on lme regular recipe agar. Next round of agar I'll go half lme to try to get heartier growth.





It is definitely isolated from the edges of that plate.


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"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
Read more, post less!
...turns out, I'm an amateur at everything

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Re: AGAR ENVY! (Anything and All things agar!) [Re: KlarnEvil9] * 1
    #29316154 -

Yea im not sure i understand what you mean by isolated. That word could have a few potential meanings


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

Edited by tryptkaloids (08/17/25 04:06 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #29316188 -

Maybe monoculture would be a better term. I'm just going to clone something from the next good pinset. I was trying to get fast rhizomorphic growth and that transfer is lacking that.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Centerfinger]
    #29316272 -

dont worry about that.  just grab a good clone and run it.  eyes on the prize.


--------------------
The Hitchhiker's Guide to the Shroomery
Visit our cultivation museum
The Hitchhiker's guide Pt. 3

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Centerfinger]
    #29316425 -

Centerfinger said:
Maybe monoculture would be a better term. I'm just going to clone something from the next good pinset. I was trying to get fast rhizomorphic growth and that transfer is lacking that.


Not a better term.

I think of a monoculture in this instance as being a monokaryon versus a dikaryon.

And if you don't know what those are... a quick google search can tell you everything you didn't know you wanted to know about it :super:


I would say that it appears you've isolated the mycelium you wanted away from contamination.

And remember, rhizo growth isn't the be all and end all of culture work. People romanticize it too much


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
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Re: AGAR ENVY! (Anything and All things agar!) [Re: LotKid]
    #29316454 -

Eyes on the prize, and your hand on your balls. Pick one,the hand doesn't matter.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Fullcircle1] * 1
    #29316478 -

Both isolates and monocultures arent very useful for most of us


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids] * 2
    #29316552 -

i do love a ropey plate but my best culture is tomentose af


--------------------
The Hitchhiker's Guide to the Shroomery
Visit our cultivation museum
The Hitchhiker's guide Pt. 3

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Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefExtreme]
    #29316556 -

fp+ is tomentose too come to think of it


--------------------
The Hitchhiker's Guide to the Shroomery
Visit our cultivation museum
The Hitchhiker's guide Pt. 3

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Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefExtreme]
    #29316565 -

Rustywhyte is another culture that is usually tommy but I've had rhizo cultures too

One of the best cultures I had looked like mold wasn't even tomentose really. Can't remember if it was a rustyhwyte clone or something else


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∴Genetics Info∴Ps. ovoideocystidiata and Native Americans∴

∴The Official Woodlovers Thread∴Outdoor Mushroom Cultivation∴

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Re: AGAR ENVY! (Anything and All things agar!) [Re: catnip40] * 3
    #29316566 -

it really makes no difference.  rhizo cultures are just more photogenic.


--------------------
The Hitchhiker's Guide to the Shroomery
Visit our cultivation museum
The Hitchhiker's guide Pt. 3

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Re: AGAR ENVY! (Anything and All things agar!) [Re: catnip40]
    #29316573 -

Roger that, rhizo growth was all the rage a decade ago. I'm just returning after a five year hiatus, but both of my comeback boxes that recently fruited were tomentose.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Centerfinger]
    #29316577 -

i mean i think we all still wish rhizo cultures were the ones we love.  much easier to tell when theyre clean.


--------------------
The Hitchhiker's Guide to the Shroomery
Visit our cultivation museum
The Hitchhiker's guide Pt. 3

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Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefExtreme]
    #29316910 -

BeefSupremeJr said:
it really makes no difference.  rhizo cultures are just more photogenic.


Ive gotten a mold that looked close to tomentose growth until it aged and turned more gray in color. Saving grace was it popped up on the edge of the plate, so was immediately suspicious. It still makes me nervous that someday that could be enmeshed with mycelium and Id struggle to spot it. Ropes give me more confidence for now.


--------------------


Hitchhikers Guide to The Shroomery

Shine on forever, shine on benevolent son
Shine down upon the severed
Shine until the two become one

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Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefExtreme] * 1
    #29316913 -

The mycelium on almost all my dishes is super thick and fluffy, its almost going aerial on me

Gandalf germ plate from spore swab inoc on 8/8/25



x7x+ T1 plate 8/12/25



Is this thickness normal?


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All that you see in front of you is how you feel inside your head. - AW



Edited by Mashiro (08/18/25 08:37 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mashiro] * 1
    #29316927 -

Mashiro said:
The mycelium on almost all my dishes is super thick and fluffy, its almost going aerial on me

Gandalf germ plate from spore swab inoc on 8/8/25



x7x+ T1 plate 8/12/25



Is this thickness normal?


How much DME(dry malt extract)?


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kloakk]
    #29316932 -

Oof, wish I knew.  I'm using the premade LME from Rak Media.  The sediment in the gandalf plate is due to me not mixing the it good enough before sterilization.  The second time I made plates, I was much more thorough during mixing and had zero sediment.


--------------------
All that you see in front of you is how you feel inside your head. - AW



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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mashiro] * 1
    #29316951 -

Mashiro said:
The mycelium on almost all my dishes is super thick and fluffy, its almost going aerial on me

Gandalf germ plate from spore swab inoc on 8/8/25



x7x+ T1 plate 8/12/25



Is this thickness normal?


Yeah.

Wanna save some money on pre mixed media?

Grab some dry malt extract and some agar powder... mix the two at 1:1 ratio. Now just use 20g of powder per 500ml of water.


--------------------

:mushroom2: LotKid's PE Tips        :shpongle:    LotKid's Chocolates :mushroom2:
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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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