anders said:

I wouldn't know how much of either to use, when.

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anders said:

misterswisher said:

Water agar is usually referring to agar with no added nutrition. Mycelium will travel out in search for the nutrients. It’s how you’re supposed to ship genetics from the MP.

And great for getting the mycelium away from contamination.

Low nutrient agar can be used for the same affect, the mycelium should run away from the bacteria faster(should) but there’s better ways to de contaminate than just using low nutrient agar.

With added nutrients it’s definitely growing you may just not be able to see it with the naked eye. You’d be surprised how far past the “halo” you can find strands of mycelium.  3 days is barely time to let the culture establish after being cut, let alone to let it venture out. Give it a week.

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Reassuring.  I was very careful moving the slices, only cracking the plates enough to set the slice, and making small slices.  18 plates and only one with contamination, and it's all over.  No idea how that happened. 

Using a SAB but armholes open.  Today I sprayed the whole inside with hydrogen peroxide 3%.  After prior experiences I'm worried about this SAB.  I washed it all down with a hose a week or so ago because of prior contaminations.

I took several slices with plins and I can't tell whether they have contamination or it's just the aborted caps yet.  All plates sealed with clingwrap cut to width -- cheap and easy.  TEAM CLINGWRAP!

Looks like I'm on the right track with this 1% agar.  Maybe this will be the time I can go to grain.

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anders said:

Ok now I've developed one or two green spots on about 4 of my 1% plates.  Does no one talk about this, or am I alone?

Would it be a good idea to crack them and excise the green before it sporullates, to save the remaining cultures?  I have four quadrants each plate.

Worried that my SAB is just contaminated.  I could hold the plates vertical as I open them to cut the green.  Needless to say, flame-sterilized scalpel.

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anders said:

Ok now I've developed one or two green spots on about 4 of my 1% plates.  Does no one talk about this, or am I alone?

Would it be a good idea to crack them and excise the green before it sporullates, to save the remaining cultures?  I have four quadrants each plate.

Worried that my SAB is just contaminated.  I could hold the plates vertical as I open them to cut the green.  Needless to say, flame-sterilized scalpel.

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DV8DUG said:

Look at these plates... Machine Elf Pheno Hunt.

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misterswisher said:

Oh contamination happens. Spores fly in through your arm holes or fall off of you. It’s a thing. Use 70% iso not peroxide. Let the box settle a little longer next time maybe. It’s a learning curve you really just gotta do abunch of plates and learn that way. Anyway that’s how I did it. Separate anything you care about from contamination asap.

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misterswisher said:

DV8DUG said:

Look at these plates... Machine Elf Pheno Hunt.

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Are those all clone plates? How are you doing your pheno hunting?

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callmehippie said:

I personally wouldn't recommend a sab like that.  When I said cheap. I actually meant cheap" like a non functional expensive vendor rip off.    The soft plastic static  tarp sides defeat the purpose of still air. 

I use a cheap(15$)hard sided  120qt sterile tub with huge armholes now.  like this



  Ever since I made the switch. my success rate skyrocketed. 


If it works for you that's cool. But I definitely recommend making your own if your having any issues at all with your sab work.

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misterswisher said:

catnip40 said:

X7x+ is a psyop

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Probably, but the wedge I received pumps out fruits like no one’s business.

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Yes, psyop. The Soviets and the CIA paid me to develop it.

The CIA told me we have it backwards though, Dr. Smoke is not the sock of catnip40.
Catnip40 is the sock of Dr. Smoke.

x7x+

DERRAYLD said:

What's the thinking with placing 4 transfers on a plate so close to the edge?

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DERRAYLD said:

What's the thinking with placing 4 transfers on a plate so close to the edge?

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