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A visual reference thread for active Pans * 14
    #29293053 -

Introduction
A place to gather pan mycelium, microscopy, and fruit body photos.  Not all information needs to be included but please include as much data as you can, and try to format it for ease of eference.  If you don't have a microscope for example, add mycelium photos, and any atypical observations about their preferences.  Along with good cap, stipe, and gill shots.  Please format similar to this.  So the thread is easily searchable.

Some questions are fine for clarification and to gather any observations that the poster may not have included.  But generally, there's better places for simple questions on pan cultivation.

And if you only want to post photos of fruits, please use a different thread unless the purpose is to add information on a collection already posted.  As the purpose of this one is largely to fill knowledge gaps rather than to chat about growing and share photos.

Thanks.  -tf

Panaeolus tropicalis - unconfirmed (?)
Section Cyanescens - confirmed by microscopy
Source - bra/pheno

Pan tropicalis (possibly needs sequenced, genetics through bra/pheno).  Ran on popcorn and phase 2 compost.  1:4.  Peat based casing (like a cyan aside from temp preference).

Better morphology at 74-76f then 80f.  Knots heavily on plates.  Cords visible.




Fruits yellow under black light when dry.



And look like this.  (On other phone, will add)





Second flush.


Third.



First flush at 80f



Microscopy 3rd.  No basidia observed clearly.  Possibly two or four in these photos.




Spores and metuloids.




--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

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Re: A visual reference thread for active Pans [Re: tree frog] * 1
    #29294802 -

Panaeolus ? - Oregon, species unconfirmed
Section Cyanescens - confirmed by microscopy
Source - wild collection, Portland, OR

Ran on popcorn and phase 2 compost.  1:4.  Peat based casing (like a cyan aside from temp range).

Better morphology at 80f then 74-76.  Cords visible.  Possible sclerotia like structures on plates.

Mycelium




Outdoor fruits 70s




90s


Indoor fruits mid 70s


80


Yellow green under black light



Microscopy - Metuloids and four spored basidia visible.




--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Edited by tree frog (07/26/25 10:57 AM)

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Re: A visual reference thread for active Pans [Re: tree frog] * 1
    #29294827 -

Pulling up a chair for this.  I've nothing to contribute unfortunately but I'm definitely willing to learn!


-Kingboomer


--------------------


I'm just a clown dancing to the sins of mankind.

-Captain Spaulding

Back in business - ICBM 2.0
Cake wars III

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Re: A visual reference thread for active Pans [Re: kingboomer] * 1
    #29294838 -

Always happy to see you :camping: fam :smile:

I don't even know where the favorite button is and don't care to learn, I don't check favorites but replies :shrug:


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: A visual reference thread for active Pans [Re: tree frog]
    #29294842 -

tree frog said:
Always happy to see you :camping: fam :smile:

I don't even know where the favorite button is and don't care to learn, I don't check favorites but replies :shrug:




Always a pleasure! 


the favorite button is all the way at the bottom, kinda hidden but it's there lol


--------------------


I'm just a clown dancing to the sins of mankind.

-Captain Spaulding

Back in business - ICBM 2.0
Cake wars III

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Re: A visual reference thread for active Pans [Re: kingboomer] * 1
    #29294844 -

I said I don't care to learn.  So my weed mind ignored you :lol:


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: A visual reference thread for active Pans [Re: tree frog] * 1
    #29294848 -

:biggrin:


--------------------


I'm just a clown dancing to the sins of mankind.

-Captain Spaulding

Back in business - ICBM 2.0
Cake wars III

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Re: A visual reference thread for active Pans [Re: tree frog]
    #29295164 -

:camping:

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Re: A visual reference thread for active Pans [Re: nocap] * 1
    #29295216 -

I will get back to this soon. Cleaning the lab before pulling out the scopes again.

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Re: A visual reference thread for active Pans [Re: tree frog] * 1
    #29295297 -

Nice reference thread for active Pans. :thumbup:


--------------------
     
How I Get Stuff done. - My Reference Guide. - My Grows.

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Re: A visual reference thread for active Pans [Re: hamloaf] * 5
    #29297740 -

Happy to see this thread.  I'd like to add to this as i've been taking photos for my own reference to look back on when troubleshooting or changing variables with pans.

Spores just starting to germinate


Pan cyan MS to Agar




A good example of nutrient content differences



Pan cyan Agar transfers (mycelium will grow thicker or thinner depending on nutrient content of plates and strain - i.e. Bisporus myc is much thinner than something like BVI(pictured below)







Temperature fluctuation




Contaminated plates/not clean growth/stalled








Tray before casing-Mycelium can be thick or thin. It can look many ways but this one is very fluffy, also more moisture in substrate IME leads to thicker growth, I assume nutrient content effects this as well

High co2 low FAE fruits




Stalled/dried out pinsets/substrate
- possibly from too high of spawn to sub ratio or just not watering enough





Fruits from a bit overly wet fruiting conditions (they will turn grayish/waterlogged -different from spores dropping all over the place)





Overly dry conditions or too much airflow in a particular area (notice the cracking)




Not overly wet fruits-not sure how the moisture effects the quality of the fruits but just an observation I've made




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Re: A visual reference thread for active Pans [Re: Timestop413] * 1
    #29297810 -

Thanks for the contributions timestop!

What are the nutrients in the two plates where you're talking about nutrient differences?


--------------------
And if I only could
I'd make a deal with God
And I'd get him to swap our places
I'd be running up that road
Be running up that hill
With no problems

Extras: Filter Print Post Top
Re: A visual reference thread for active Pans [Re: tree frog] * 1
    #29298144 -

I can't recall as this photo was from a year or so ago but the one on the right is made with grain water agar(GWA), the one on the left is malt yeast agar.

I have tried various agar mixes with pans. 2%, 1%, and 0.5% (per baba yaga method) Light Malt Extract.  So per 1000ml of water, anywhere from 5 grams (0.5%) up to 20 grams (2%) of LME, thicker growth came with higher nutrient. I liked grain water for a bit but I don't always have grain water and found it more of a hassle to make.

My go to agar recipe currently is:
per 1000ml distilled water
20g agar
14g LME
1g nutritional yeast

this has worked well for the handful of active species I've tried growing.  Pan cyans, subtrops, bisporus, cubes

Edited by Timestop413 (07/29/25 11:47 PM)

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