ThurmanMurman said:

Yep ya can. Basically just take an agar transfer to sterilized water, and you've got long term storage. Thanks for the reminder - I need to order cryovials!

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mushboy said:

nektar61 said:

they're not needed unless you're doing a commercial grow.

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why limit yourself?

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mushboy said:

nektar61 said:

they're not needed unless you're doing a commercial grow.

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why limit yourself?

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AZ Starman2112 said:

ThurmanMurman said:

Yep ya can. Basically just take an agar transfer to sterilized water, and you've got long term storage. Thanks for the reminder - I need to order cryovials!

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I just looked at cryovials. The 5ml. Not sure how much they hold, but it has to be better storing agar a samples from clean plates in those in the refrigerator than a bunch of plates I would assume? I am about runner over with plates ATM and hate to toss some of these as they are really pretty and clean.

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Phaethon said:

AZ Starman2112 said:

ThurmanMurman said:

Yep ya can. Basically just take an agar transfer to sterilized water, and you've got long term storage. Thanks for the reminder - I need to order cryovials!

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I just looked at cryovials. The 5ml. Not sure how much they hold, but it has to be better storing agar a samples from clean plates in those in the refrigerator than a bunch of plates I would assume? I am about runner over with plates ATM and hate to toss some of these as they are really pretty and clean.

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You don't even need to store them in the fridge. Just put distilled water in them, sterilize, and add a few pieces of colonized agar. You can keep them at room temperature.

I use the 2ml, for which you can also find convenient 100-spot storage boxes on Amazon. The 5ml ones will work just fine too.

Check out Mycoplex's Cryovial Tek. And also ReverendMyc's LI storage tek.

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Great info and thanks! I will read those threads on my next break here at work.

AZ Starman2112 said:

So are you guys saying to avoid LC or the process of storing myc covered agar in small pieces in cryovials containing sterilized distilled water?

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ThurmanMurman said:

AZ Starman2112 said:

So are you guys saying to avoid LC or the process of storing myc covered agar in small pieces in cryovials containing sterilized distilled water?

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They're discussing the pitfalls/necessity of LC.

Storing cultures in distilled water is groovy af all the time.

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Well then I’m about to get groovy! Especially since no refrigeration is needed.

nektar61 said:

mushboy said:

nektar61 said:

they're not needed unless you're doing a commercial grow.

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why limit yourself?

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For the same reasons I'd suggest a new grower not try to "experiment" until they've grown with proven teks and got them down. Cart before horse. 

People will do what they're going to do, and LC seems fun, but it seems to result in contam with new growers, or new growers not sure if they have contam, or just tossing spores into broth.

I think you and I have had this discussion before. It not, it was something similar.

I'll keep suggesting people don't do it at first and you keep questioning it.

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Phaethon said:

I'm with you on discouraging new growers from trying to use LC as a shortcut - notably syringe to broth is asking for trouble, even with LC, and unfortunately there are a number of vendors advertising exactly that: buy this LC syringe and expand it straight into to a much larger amount of LC.....I think a better principle might be something like "Don't try making LC before you can consistently get (and recognize!) clean culture on agar", and never as a shortcut on an early grow.

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Phaethon said:

"Don't try making LC before you can consistently get (and recognize!) clean culture on agar", and never as a shortcut on an early grow.

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cybesa said:

Why is it preferable to Slants?

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Smellyhobbit said:

I fully understand that I am in an extreme minority here, but I just want to say LC and SSLC are identically easy to inoculating grain. If you can put a wedge of agar to grain, you can put a wedge of agar to a bottle of juice. In fact, it’s much much easier to make the broth for SSLC than it is to make good grains.

Insofar as using it, it’s even easier than agar to grain. Just pour a little sauce in your nugget box and you are rocking and rolling.

SSLC isn’t just a tool for bulk growing, but a great way to explore the phenos hidden inside a culture. As Piggy said, it’s 3D agar, essentially. So where a plate sectors in 2 dimensions, SSLC (or just regular LC) expands in 3. Globes instead of circles.

I understand to a degree why we steer people away from it, but really if you can do a clean agar transfer to a new plate you’re already doing more complex movements than LC. I think liquid culture and agar can honestly be learned in tandem. I know that’s what I did, and I’m not special or more adept than any other new grower here.

It’s just a bottle of broth. Genies don’t come out when you open them or anything. A2G is the same thing as A2broth, but the broth is easier to prep.

Ok. Eviscerate me now. I am ready.

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Gloves on baby.

I mean I don't see you disagreeing with what most experienced people are saying about LC, SSLC, ECT.  In the scenario you have presented the cultivator can already develop and identify a healthy clean culture on agar to transfer into LC.  By that point yes, they are ready for LC.  So, in essence, you have agreed with what most people are already saying and are not an outlier on the issue.

I think what most people are saying is there's this new craze about LC and newbies are trying to jump right into it without any experience or understanding what they are doing, often with skipping agar altogether.

For just a second I had an issue with the more phenos in 3d than 2d as it's the exact same thing just flat or globular, but then my brain did the rest of the math and that globe broken up into an LC is going to have more phenos than a tiny agar wedge taken from a plate.

TLDR: I think you're mostly agreeing with everyone