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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap] * 2
    #28745267 -

Stipe-n Cap said:


:lol:

5 more to go:




One more lost to bacteria:



One to mold:



But I've spotted what appears to be clean germination on another plate:



7th day since inoculation with 3yo dried gill fragments from fruits dried longer than 24 hours at 160Β°F.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap] * 2
    #28745549 -

Natalensis spores on agar, only 4 days old


Also a few transfers I took from BRF pucks.


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The Hitchhiker's Guide to the Shroomery

Using Ziplocs (Ziplocks) as Fruiting Chambers

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap] * 2
    #28746492 -

Germination confirmed:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28746509 -

Nice that's kind of amazing really they survived the dehydrator blasting. Wonder if it means the ones that survived and germinated have a genetic disposition to be more resilient spores.


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Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69]
    #28746514 -

What do you think of this spore germination plate?

Am I looking at two different set of cubensis mycelium that aren’t getting along very well? Or do you think contamination is a factor?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69] * 1
    #28746523 -

I suspect that spores are just hardier than expected. Plenty of folks have put caps or clones to agar in the past and have seen growth. I suspect that the growth is from spores and not from dried fruit material due to the temperatures reached during drying, which should kill live mycelium.

If the mycelium survived I would have expected growth sooner than 8 days and for the growth to be appear more structured.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SonomaFungi_707]
    #28746534 -

SonomaFungi_707 said:
What do you think of this spore germination plate?

Am I looking at two different set of cubensis mycelium that aren’t getting along very well? Or do you think contamination is a factor?




Looks good take some transfers from that 3 o'clock and noon regions to some new dishes.


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Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Stipe-n Cap]
    #28746535 -

Stipe-n Cap said:
I suspect that spores are just hardier than expected. Plenty of folks have put caps or clones to agar in the past and have seen growth. I suspect that the growth is from spores and not from dried fruit material due to the temperatures reached during drying, which should kill like mycelium.

If the mycelium survived I would have expected growth sooner than 8 days and for the growth to be appear more structured.


I think that's a safe assumption. Very doubtful that's the dried flesh coming back to life.


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Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69]
    #28747732 -

How does my germ plate look?

Should i send it or take a transfer?





Btw the circled thing is a dirt stain on the lid.



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:heart: πŸ…ƒ πŸ„΄ πŸ„° πŸ„ΌΒ Β  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ :heart:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Yahra] * 3
    #28747736 -

Hard to tell tbh but dont ever send a germ plate


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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator]
    #28747739 -

Why not, if it looks clean?


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:heart: πŸ…ƒ πŸ„΄ πŸ„° πŸ„ΌΒ Β  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ :heart:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Yahra] * 2
    #28747743 -

Yahra said:
How does my germ plate look?

Should i send it or take a transfer?





Btw the circled thing is a dirt stain on the lid.




I have a hard time understanding what's going on on that plate. I would make a tiny transfer from one of the rhizo growths


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The Hitchhiker's Guide to the Shroomery

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Yahra] * 4
    #28747744 -

You cant rely on your senses (sight, smell, etc) to judge whats going on in the microscopic world fungi operates in.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator] * 3
    #28747749 -

That's right, you can only use your ESPN


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Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69]
    #28747751 -

My what?

:utterconfusion:


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:heart: πŸ…ƒ πŸ„΄ πŸ„° πŸ„ΌΒ Β  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ :heart:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Yahra] * 4
    #28747784 -

ESPN is an american sports channel, it's a play on words instead of saying ESP (extra sensory perception)

You're German, I'm autistic, it's practically the same thing.  Allow me to translate.


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πŸ…ƒπŸ„΄πŸ„°πŸ„Ό πŸ„ΈπŸ„·πŸ„ΎπŸ„Ώ


New to cultivation or the shroomery?  Observe the hitchhiker's guide

The Hitchhiker's Guide to the Shroomery



Smellyhobbit said:
"Wiping a needle with isopropyl after flame sterilization is like shitting after you wipe your ass."

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mr Piggy] * 1
    #28747802 -

I am a German with Asperger's. Sometimes i have a hard time here.

:cool:

But fun times nevertheless.


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:heart: πŸ…ƒ πŸ„΄ πŸ„° πŸ„ΌΒ Β  πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ :heart:


Edited by Yahra (04/24/24 12:22 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Yahra]
    #28748544 -

Thinking it's time for a final check and then to drop these into some grain, top photo is transfers from one grem plate and bottom photo from another. All P. Nats.

Germ plate 2 transfers, first plate, top left had some suspect spots, have some transfers growing out of that plate, not 100% sure if it was bacteria or because agar wasn't 100% solid when I moved these plates and the surface just looked weird.

I guess I should let plate 4, bottom right and maybe plate 2, top right, grow out some more? Plate 4 is being especially slow :rolleyes:

Germ Plate 2 transfers:


Think these plates from Germ plate 1 are both behaving themselves :cool:.

Grem Plate 1 transfers:


I have 8 x quart jars of grain ready to go, going to run 8 x 5L shoe boxes, 1 quart per shoe box, not sure how to best split these up...

I was thinking of maybe splitting the 3 plates that have filled the whole plate into half and drop into 2 jars per plate, using 6 of the 8 jars.

Then the entire plate, for the other 2 plates into 1 jar each?

Guessing I should toss the potentially suspect plate from the Germ Plate 2 lot of transfers, and then use those 2 transfer from that plate for another couple of jars... ideally I'd fit everything into the 8 shoe box run, but I guess I could then use those 2 jars to do a monotub?

As always appreciate your input shroomery. Thank you.

Edited by Dkeppel (04/24/24 09:36 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Yahra] * 3
    #28748563 -

Mr Piggy said:
You're German, I'm autistic, it's practically the same thing.  Allow me to translate.


Yahra said:
I am a German with Asperger's. Sometimes i have a hard time here.


:rofl:

:nodofunderstanding:


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Official Ps papuana Cultivation Thread
exotic indexes: Ps zapotecorum varieties, Ps papuana
my grow logs: Ps neoxalapensis, Ps baeocystis, Ps papuana

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 3
    #28748708 -


Thanks nektar61! :grin: Took a couple of arbitrary transfers.


This was from a clone of a leucistic, purple-spored fruit from a Rusty Whyte multispore grow that seems to have been an accidental cross. Its tub produced wild-type mushrooms and leucistic mushrooms with purple spores, but none with brown spores. Every time I've grown out the clone culture, it makes only purple (or maybe they're mixed purple and brown?) spores. This large plin dropped very distinctly brown spores! I transferred a couple chunks of the stipe to new plates.

(I guess maybe it's notable that this culture likes to make two very different sectors on agar, even when I transferred from just one of those sectors:

So maybe had just gotten unlucky with a transfer and lost the brown-spored strain on the actual lineage I've grown out?)


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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