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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycolorado]
    #28488859 -

enscred1312 said:


Struggling with bacterial jars and trying to troubleshoot my whole process.
What do you think of these plates ? T3 from a clone (cubensis).



:snoopyes:


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids] * 1
    #28488884 -

My most recent experience-producing event: streaking on soft agar makes a really big mess.

Can I get a second opinion on these? I'm thinking contam.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: rezidualfx] * 1
    #28488889 -

I wouldn't take from them personally


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rezidualfx] * 1
    #28488897 -

rezidualfx said:
This is one of my ADHD-powered plin plates– neglect something long enough and it just might fruit





Oooo I’d clone that bad boy in a heartbeat


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 1
    #28488982 -

Thanks, I appreciate the feedback!

Now I'm just hoping these APE plates aren't fucked otherwise I'm gonna have to track down some more source material

I dropped 1-2cc of LC on this plate to use as a reservoir for streaking:



...and here's a couple of the recipient plates



I went for a z-shape when streaking these plates, which seemed to work a bit better on the soft agar

Smellyhobbit said:
rezidualfx said:
This is one of my ADHD-powered plin plates– neglect something long enough and it just might fruit





Oooo I’d clone that bad boy in a heartbeat



That's the plan!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rezidualfx] * 1
    #28488987 -

ape looking hopeful. :thumbup:


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #28489064 -

Some clear winners for first non-germ plate transfers here but are the rest just mold? Or are some tom?

Maketi :smile:







--------------------
Thanks to everyone for sharing what they know :smile:. 165F for 24hrs.

Better Holes Tek (Use a Step Drill Bit)
Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycolorado] * 1
    #28489261 -

Just made spore suspensions for:
  • Ps. Hispanica
  • Ps. Cubensis (Stargazer) × Ps. Natalensis [F1]

Ps. Hispanica is from a spore print.



This is the second of two Hispanica spore prints I had. The first one I failed to get any germinations from so am having another shot at it. I dropped the spore print into 2ml of sterile distilled water in a test tube with a steel ball bearing. I shook and vortexed the tube then used a sterile pipette to transfer the suspension into another test tube.

The spore print is on card and when shaking it (less so vortexing) it decimated the card. When I was counting the spore concentration with a haemocytometer and microscope it was very difficult due to there being so much paper debris. Perhaps this will affect germination in some way. The card may contain toxic glues. Please don't use card for spore prints. The spore concentration is 1,875.0 single spores per drop (assuming 0.05ml per drop) with an additional 0.33 clusters of spores per single spore.

Inoculated 4 petridishes of different recipes each with 5 drops of the spore suspension in a crosshair formation. If I get any successful germination then I will do a serial dilution and inoculate more plates to try to isolate a monokaryotic culture.



Ps. Cubensis (Stargazer) × Ps. Natalensis [F1] used a very sparse looking swab. I pulled the end of the swab away from the wooden stick using tweezers and snipped it into 3ml of sterile distilled water in a test tube with a steel ball bearing. I shook and vortexed the tube and then used a sterile pipette to transfer the suspension into another test tube. In the final test tube there is about 2.25ml of suspension since the swab absorbed some of the liquid.

The spore solution for this one was much cleaner, I like working with swabs, and the concentration was higher than I expected. There are 5,062.5 single spores per drop (assuming 0.05ml per drop) with an additional 0.23 clusters of spores per single spore.

Inoculated 4 petridishes of different recipes each with 1 drops of the spore suspension mostly just to test it's germination and potential contamination rate. I'm planning to send the spore suspension directly to spawn for top fruiting to select an F2.


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Edited by covertjoy (10/01/23 07:02 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: covertjoy] * 1
    #28489359 -

Apparent Yeti really likes peptone because this plate is growing super thick.
Swab plate:


And then T1:


Meanwhile a failure from the same swab:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVadisx] * 1
    #28489692 -

xVadisx said:
Some clear winners for first non-germ plate transfers here but are the rest just mold? Or are some tom?

Maketi :smile:









That maketi was an F1 cross clone, so you’re going to see a ton of weird myc and an equal amount of variation in the phenotypes between yeti and mak… you’re growing out the f2 of a cross (it’s literally the most unstable a cross can be lol)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster] * 1
    #28489781 -

Jack Frost PDY T4 9/28/23
1) 2)

Edited by huey.bluey (10/02/23 08:55 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: huey.bluey] * 3
    #28489788 -

A wacky T1. This is my first attempt at a simple cross. Two sets of spores on one swab. Would you take from this plate, and what looks promising to you if so?



--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Smellyhobbit] * 3
    #28489805 -



--------------------
Astra said:

“Peace River has a gentle personality, yet TTBVI hit me like strong LSD with a twist of medical grade meth. Not for the faint of heart.”

Team Panaeolus

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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster] * 1
    #28489942 -

fahtster said:
That maketi was an F1 cross clone, so you’re going to see a ton of weird myc and an equal amount of variation in the phenotypes between yeti and mak… you’re growing out the f2 of a cross (it’s literally the most unstable a cross can be lol)



I’m glad I saw this explanation,  because now this makes so much more sense to me. I have like 8 transfers from 2 diff germ plates because I was scratching my head on what was / wasn’t cube mycelium

So far I have one extremely slow growing mycelium and one faster one. The slower one is thicker and more rhizo, while the faster is more tomentose and fluffy. Pretty wild looking germ plates. It’s the first time I swabbed a plate and second guessed if every colony was mold

Thanks Faht :rockon:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster]
    #28490068 -

fahtster said:
xVadisx said:
Some clear winners for first non-germ plate transfers here but are the rest just mold? Or are some tom?

Maketi :smile:









That maketi was an F1 cross clone, so you’re going to see a ton of weird myc and an equal amount of variation in the phenotypes between yeti and mak… you’re growing out the f2 of a cross (it’s literally the most unstable a cross can be lol)



Gotcha!! I’ll take some more transfers from the plates I wasn’t as sure about. I see a lot of bags and shoeboxes in my future to isolate winners :smile:. Excited to share results!


--------------------
Thanks to everyone for sharing what they know :smile:. 165F for 24hrs.

Better Holes Tek (Use a Step Drill Bit)
Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY

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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVadisx]
    #28490208 -

Penis envy biopsy tissue on water agar



Looking awesome. Cant wait to transfer and see what it looks like grown out


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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator]
    #28490255 -



6 varieties I've never grown before plus Golden Mammoth which I only grew once during my first grow. These are hopefully gonna turn into prints and swabs to do a big giveaway, so keep your eyes out for that in like... 3-6 months :lol:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Benson]
    #28490271 -

Benson said:


6 varieties I've never grown before plus Golden Mammoth which I only grew once during my first grow. These are hopefully gonna turn into prints and swabs to do a big giveaway, so keep your eyes out for that in like... 3-6 months :lol:



Did you use fresh spores for these? I swabbed some plates this weekend and I didn’t see any black streaks and last time this happened they didn’t grow. So just wondering if all the spores I have are old or what gives.


--------------------
“Sunny days wouldn't be special if it wasn't for rain.” – Curtis Jackson
“There is a world beyond ours, a world that is far away, nearby, and invisible.” – Maria Sabina

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar] * 5
    #28490309 -

been getting so much shit for this in my disco i had to come post it.  laugh it up, fuzzballs, but this is the ideal media bottle setup. you may not like it but this is what peak performance looks like.




--------------------
Need a lift?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: BeefExtreme]
    #28490318 -

My DIY hobby lobby agar punch for less than 8$

PC ready or boil it in water for 5 minutes for a faster sterilization.


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