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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator]
    #28473939 -

What you guys think of this plate?
Mold?


Can someone explain to me why does this occur?


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"I have realized that the past and future are real illusions, that they exist in the present, which is what there is and all there is.”
― Alan Wilson Watts


Edited by FoTwenty (09/18/23 06:54 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: FoTwenty]
    #28473940 -

Looks like a plate I'd transfer from. I think.


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Crack's Tek's and Shit


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa]
    #28473946 -

I've transferred like 5 times from a plate that looked like this. Then all of them dint looked like this. And suddenly this occurred again...


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"I have realized that the past and future are real illusions, that they exist in the present, which is what there is and all there is.”
― Alan Wilson Watts


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Re: AGAR ENVY! (Anything and All things agar!) [Re: FoTwenty] * 1
    #28473971 -

I’m pretty sure I figured this one out a while ago.


So the next tiem you transfer, pay attention to what side of the wedge was the leading edge of the myc growth, and which sides were from further down. In my case that weird spot was always starting from the side of the wedge that was the edge of the myc growth.


Could just be mine, but I’d bet it’s something similar. This also happens when there’s mold meshed in but it looks a little different. Yours looks all clean to me.



It could also be from something like the wedge not sitting flush on the plate. Like if one side is slightly tilted up off the plate it might take the mycelium a little longer to grow down to the surface before spreading out whereas the rest of it just has to grow outward because it was already touching surface.


Idk why but this used to drive me insane to the point I had to find an answer :lol:


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LAGM2020

Edited by A.k.a (09/18/23 07:22 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: A.k.a]
    #28473980 -

I'm gonna upload some better pics now


--------------------
"I have realized that the past and future are real illusions, that they exist in the present, which is what there is and all there is.”
― Alan Wilson Watts


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Re: AGAR ENVY! (Anything and All things agar!) [Re: FoTwenty]
    #28473987 -



--------------------
"I have realized that the past and future are real illusions, that they exist in the present, which is what there is and all there is.”
― Alan Wilson Watts


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Re: AGAR ENVY! (Anything and All things agar!) [Re: FoTwenty]
    #28473993 -

Insane you say... I'm dying over here doing my research to why this is happening and what is it...

The new pics looks uniform under the agar where the suspicious area is..


--------------------
"I have realized that the past and future are real illusions, that they exist in the present, which is what there is and all there is.”
― Alan Wilson Watts


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Re: AGAR ENVY! (Anything and All things agar!) [Re: FoTwenty]
    #28474013 -

FoTwenty said:



It looks like something is riding along with the wedge you transferred.
Try transferring solely mycelium to a new plate and leave the old agar on the plate.
I did this recently and the new plate was much more uniform/circular growth.
Is your nutrient composition the same on each plate? Different agar batches?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroMycelium] * 1
    #28474017 -

I This transfer came from a very uniform and healthy looking plate.
I put a piece from the healthy looking one on some grain . I will wait for the first shake and see if it goes bad or not.


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"I have realized that the past and future are real illusions, that they exist in the present, which is what there is and all there is.”
― Alan Wilson Watts


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Re: AGAR ENVY! (Anything and All things agar!) [Re: FoTwenty]
    #28474023 -

This agar batch was 6g lme 500ml.
What do you mean diferent nutrient composition?

If I swirl the bottle each pour?
I turn of the stirplate when it's about temp.


--------------------
"I have realized that the past and future are real illusions, that they exist in the present, which is what there is and all there is.”
― Alan Wilson Watts


Edited by FoTwenty (09/18/23 08:12 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: FoTwenty]
    #28474025 -

FoTwenty said:
I This transfer came from a very uniform and healthy looking plate.
I put a piece from the healthy looking one on some grain . I will wait for the first shake and see if it goes bad or not.


Do you remember the direction of the scalpel transferring the piece?
Some people make a habit of flaming their scalpel handle as they believe it can allow contamination to ride along/fall on the new plate while transferring since typically only the blade is sterile.

Ex. The afflicted area in that photo is to the left, good growth to the right, so perhaps hovering the handle over the left side while placing the new piece allowed something undesired to fall onto that side :shrug:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroMycelium] * 1
    #28474029 -

Taking note of directionality of agar wedges during transfers now.

In other news, whatcha think of this plate (minus the small rant)? I think I've got a new LC candidate? Maybe? In any case it makes me happy to see a nice even circular growth. Yay. Hopefully. :boofbanana:



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Thanks to everyone for sharing what they know :smile:. 165F for 24hrs.

Better Holes Tek (Use a Step Drill Bit)
Don't forget your towel: THE HITCHHIKER'S GUIDE TO THE SHROOMERY

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Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroMycelium]
    #28474032 -

I flame the tip (gigity) and all on the area that hovers the plate


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"I have realized that the past and future are real illusions, that they exist in the present, which is what there is and all there is.”
― Alan Wilson Watts


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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVadisx]
    #28474040 -

xVadisx said:
Taking note of directionality of agar wedges during transfers now.

In other news, whatcha think of this plate (minus the small rant)? I think I've got a new LC candidate? Maybe? In any case it makes me happy to see a nice even circular growth. Yay. Hopefully. :boofbanana:




Looks great to me!


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids] * 1
    #28474086 -

If it's meshed in mold.. when thrown into grain, what indicators I will have to be on the lookout for. If it's mold,  shouldn't be growing exponentially fast.?


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"I have realized that the past and future are real illusions, that they exist in the present, which is what there is and all there is.”
― Alan Wilson Watts


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Re: AGAR ENVY! (Anything and All things agar!) [Re: FoTwenty] * 8
    #28474311 -

Ghost


Country Cock

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tholos]
    #28474321 -

tholos said:
Ghost


Country Cock



:bow2: What’s the recipe? I just been doing 10g agar, 10g LME, 400 ml water but I think I want to try less LME maybe 7gs.


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“Sunny days wouldn't be special if it wasn't for rain.” – Curtis Jackson
“There is a world beyond ours, a world that is far away, nearby, and invisible.” – Maria Sabina

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar] * 2
    #28474339 -

0mar said:
tholos said:
Ghost


Country Cock



:bow2: What’s the recipe? I just been doing 10g agar, 10g LME, 400 ml water but I think I want to try less LME maybe 7gs.


16g LME, 20g Agar, 1g Nutritional Yeast, 1000mL H2O

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tholos]
    #28474739 -

Day 3 of t1 on these ghost plates other one looks kinda meh so I’ll be focusing on this one… I THINK I might have done something boys & girls I know it’s early but what do we think? Also the ksss ufos are showing zero growth which sucks but at least I know my tek is somewhat clean.



Nvm I think it’s just mold again :sad:


--------------------
“Sunny days wouldn't be special if it wasn't for rain.” – Curtis Jackson
“There is a world beyond ours, a world that is far away, nearby, and invisible.” – Maria Sabina

Edited by 0mar (09/19/23 12:16 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar]
    #28474805 -

Your technique looks clean and the plates certainly look clean at this point.
Just start over with some new spores.

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