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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator]
    #28454889 -

Man I’m so confused now :confused:
These are some of the plates that I inoculated but didn’t transfer from maybe this will give everyone a better idea of what it really is … now that I’m looking I think it is mold. Huge bummer but I’m still learning so I guess it comes with the territory :facepalm:



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“Sunny days wouldn't be special if it wasn't for rain.” – Curtis Jackson
“There is a world beyond ours, a world that is far away, nearby, and invisible.” – Maria Sabina

Edited by 0mar (09/01/23 12:35 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar]
    #28454939 -

0mar said:
Man I’m so confused now :confused:
These are some of the plates that I inoculated but didn’t transfer from maybe this will give everyone a better idea of what it really is … now that I’m looking I think it is mold. Huge bummer but I’m still learning so I guess it comes with the territory :facepalm:



It's never about how bad you want to succeed, only how long you are willing to persevere. Keep trying!


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Pnin]
    #28454950 -

Pnin said:
0mar said:
Man I’m so confused now :confused:
These are some of the plates that I inoculated but didn’t transfer from maybe this will give everyone a better idea of what it really is … now that I’m looking I think it is mold. Huge bummer but I’m still learning so I guess it comes with the territory :facepalm:



It's never about how bad you want to succeed, only how long you are willing to persevere. Keep trying!



Thanks man.. just a bummer my first go around I got a nasty syringe and now some bad swabs or bad tek and not knowing which is what pisses me off lol. I’m just gonna go ahead and say bad spores because my plates come out clean and stayed clean so idk back to the lab it is…


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“Sunny days wouldn't be special if it wasn't for rain.” – Curtis Jackson
“There is a world beyond ours, a world that is far away, nearby, and invisible.” – Maria Sabina

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar]
    #28454958 -

Damn omar, that sucks. I'm bummed for ya. We were T1 brothers. Hope the best for your next try
:hugitout:

If it makes you feel better I've also had bad luck with a few of my first syringes


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The most important step a man can take. It's not the first one, is it?
It's the next one. Always the next step, Dalinar.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: NotFromGeorgia] * 1
    #28454962 -

NotFromGeorgia said:
Damn omar, that sucks. I'm bummed for ya. We were T1 brothers. Hope the best for your next try
:hugitout:

If it makes you feel better I've also had bad luck with a few of my first syringes




Haha thanks NFG and that’s crazy that’s exactly what my plates looked like, yellow nasty bacteria.

I got these swabs from someone on FB I have two more sets but now idk if I want to use them…

Luckily a tc on here sent me a care package so I have something else to work with.

Gonna pour some new plates tonight and I’ll be back with a report. :rockon:


I just wish I had a better understanding of how it was supposed to look all these pictures I’ve been seeing just look so similar to my plates but again I’m so new idk what to even look for (obviously)


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“Sunny days wouldn't be special if it wasn't for rain.” – Curtis Jackson
“There is a world beyond ours, a world that is far away, nearby, and invisible.” – Maria Sabina

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar]
    #28455020 -

I think looking for organization is the important part. Like finding growth that is lining up with itself. Just as new as you are so maybe that's wrong.

This stuff looks puffy and disorganized, but light going through it shows the hyphae grouping together into lines instead of going in all random directions.


It bruises blue when transferring so I'm pretty sure it's the good stuff. But like derryld said, molds can be organized too so... maybe we'll be mold brothers soon lol


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The most important step a man can take. It's not the first one, is it?
It's the next one. Always the next step, Dalinar.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: NotFromGeorgia]
    #28455058 -

Is the top picture mold? Or are these good plates. To my eyes they look good :confused:


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“Sunny days wouldn't be special if it wasn't for rain.” – Curtis Jackson
“There is a world beyond ours, a world that is far away, nearby, and invisible.” – Maria Sabina

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar]
    #28455082 -

both the same plate, I think they're good


--------------------
The most important step a man can take. It's not the first one, is it?
It's the next one. Always the next step, Dalinar.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar]
    #28455084 -

A few days later on these APE plates and I think I'm cookin with gas, but tell me if I'm wrong.



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Hitchhiker's Guide to the Shroomery

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Re: AGAR ENVY! (Anything and All things agar!) [Re: NotFromGeorgia]
    #28455101 -

Hell ya brotha looks good to me to but I don’t know shit :hehehe:


--------------------
“Sunny days wouldn't be special if it wasn't for rain.” – Curtis Jackson
“There is a world beyond ours, a world that is far away, nearby, and invisible.” – Maria Sabina

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar] * 1
    #28455115 -

It looks like you've got something going there, but it needs to be transferred at least once.  It doesn't look very good in its current condition.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28455273 -



Making transfers from a Psilocybe Cyanescens germination plate to water agar.

The germination plate was inoculated using a pipette with spore solution made from a spore print. Each drop would contain approximately 20,500 spores.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Kinoko314]
    #28455499 -

Kinoko314 said:
It looks like you've got something going there, but it needs to be transferred at least once.  It doesn't look very good in its current condition.


Yeah planning to make some pasty plates this weekend, I'll probably transfer on Sunday.


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Hitchhiker's Guide to the Shroomery

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Re: AGAR ENVY! (Anything and All things agar!) [Re: covertjoy]
    #28455631 -

Na I think every drop would contain 500k spores


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Screwup] * 1
    #28455647 -

Screwup said:
Na I think every drop would contain 500k spores


I used a hemocytometer. Each corner grid had an average of 41 spores. Multiply that by 10,000 to get the number of spores per ml giving 410,000. A pipette drop is 0.05ml so divide by 20 to get the number of spores per drop giving 20,500.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Screwup]
    #28455652 -

Made some PDA and MEA agar plates, 87 plates in total. Read a lot about mycelium doesn't like red color, so I bought pink.. But it's still red 🍄
PDA isn't as translucent as MEA, at least not mine. Black and deep Green and Blue is my favorite because the mycelium really pops out. How do I get rid of the foam that is in the agar agar plates?


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Understanding is enlightening, always be open to fresh input and diversity! Be a better man respect others as you would like to be respected.


"The greatest journey of exploration is to understand our own minds. #Exploration #Understanding #Mind

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Re: AGAR ENVY! (Anything and All things agar!) [Re: covertjoy]
    #28455668 -

covertjoy said:
Screwup said:
Na I think every drop would contain 500k spores


I used a hemocytometer. Each corner grid had an average of 41 spores. Multiply that by 10,000 to get the number of spores per ml giving 410,000. A pipette drop is 0.05ml so divide by 20 to get the number of spores per drop giving 20,500.


Smh couldn’t have included that info before?


--------------------
🅃 🄴 🄰 🄼    🄲 🄻 🄸 🄽 🄶 🅆 🅁 🄰 🄿


Help US help YOU TEK

2023 Dehydrator TEK

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Re: AGAR ENVY! (Anything and All things agar!) [Re: covertjoy] * 1
    #28455707 -

covertjoy said:
Screwup said:
Na I think every drop would contain 500k spores


I used a hemocytometer. Each corner grid had an average of 41 spores. Multiply that by 10,000 to get the number of spores per ml giving 410,000. A pipette drop is 0.05ml so divide by 20 to get the number of spores per drop giving 20,500.


I'm something of a scientist myself


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The fool who persists in his folly will become wise.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28455895 -

How long do you wait to inoculate fresh plates? When it’s cool or do you give it a week to see if they are contaminated? My last go around I just left them in my SAB let them cool and gel then I inoculated them the same day. I have a plate from over a month ago and it’s still clean. Just looking to see what everyone else is doing. Currently making more agar rn to overcome the huge L that was my second go around.


--------------------
“Sunny days wouldn't be special if it wasn't for rain.” – Curtis Jackson
“There is a world beyond ours, a world that is far away, nearby, and invisible.” – Maria Sabina

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 0mar]
    #28455899 -

It depends on your confidence level pouring plates.

I trust my plates as soon as they are cool, others may not be so trusting.


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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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