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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: MicroMycelium]
    #28413243 -

mushboy said:
i didnt think they still made nos.

what a throwback:lol:


I don't think they make the bottles with the cool caps anymore tho.
MicroMycelium said:
How long do you wait before you’re confident using new plates you poured?
I know you can inoculate your new plates as soon as they cool, but if they got contaminated during pouring that would take a day or two or three to be visible so avoiding initial contams would obviously be a bonus.


If you're germinating spores, id wait a 3 days atleast to be sure any bacteria that may have made it in germinates first, but if you're transferring to it, fuckit just keep the original culture. :shrug:
Contams getting in during a pour is pretty damn rare ime, and it's something I've never given any concern towards


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"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids] * 4
    #28413756 -

Used some molasses as a substitute for corn syrup in my PDA plates cause I didnt wana go back to the store. In combination with the blue food coloring they came out to a very pretty green, semps seem to like it too



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Re: AGAR ENVY! (Anything and All things agar!) [Re: milkboy] * 3
    #28413947 -

Hillbilly pumpkin clone plates


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Whyterye] * 1
    #28414740 -

New 120mm plates

90 vs 120

So nice


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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #28414747 -

Why stop there? 200MM PLATES INCOMING


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Screwup] * 1
    #28414751 -

Nah, there are limits to the madness.

Beyond 120 and the glass is too thin for my liking.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD] * 5
    #28414812 -

i find the larger ones are a bitch to pour in stacks.

my fav size for just everyday working is 60mm and i dont waste agar like i do with larger dishes.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #28414835 -

Green stuff showed up in the middle of and underneath mycelium after about 10 days. So sneaky.


Was that bottom sector's strained looking growth a warning sign?

Edit: Could have been between 10-14 days


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Edited by Arctic_Fox (07/31/23 10:18 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: mushboy]
    #28414872 -

mushboy said:
i find the larger ones are a bitch to pour in stacks.

my fav size for just everyday working is 60mm and i dont waste agar like i do with larger dishes.



100%

I do no pour so these work very well for me.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox]
    #28414895 -

Arctic_Fox said:
Green stuff showed up in the middle of and underneath mycelium after about 10 days. So sneaky.


Was that bottom sector's strained looking growth a warning sign?

Edit: Could have been between 10-14 days


That bottom section is mold 100%


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #28414911 -

tryptkaloids said:
Arctic_Fox said:
Green stuff showed up in the middle of and underneath mycelium after about 10 days. So sneaky.


Was that bottom sector's strained looking growth a warning sign?

Edit: Could have been between 10-14 days


That bottom section is mold 100%


Oh, shit.

Now I'm fucked. I wonder what else I have that's mold. All my plates have white growth of some kind on them. I'm sure some of it is mushroom mycelium, but what else?

What about these?
1.
2.
3.


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Edited by Arctic_Fox (07/31/23 12:14 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato] * 3
    #28414929 -



:buzzaldrin:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox]
    #28414933 -

I don’t believe that is mold at all and not sure how tryp is claiming 100%.  Anyway, those plates all look like funky germ plates with the exception of 3 which looks like a good xfer that could be used after 1-2 more xfers.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycolorado]
    #28414943 -

Mycolorado said:
I don’t believe that is mold at all and not sure how tryp is claiming 100%.  Anyway, those plates all look like funky germ plates with the exception of 3 which looks like a good xfer that could be used after 1-2 more xfers.


What's with the hint of green then?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Arctic_Fox]
    #28414950 -

It’s bruising. There’s likely something riding along that it doesn’t like.  Doesn’t mean the bottom section is mold 100%.  I wouldn’t use that plate either way.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycolorado]
    #28414968 -

Looks more blue than green to me, certainly doesnt look axenic, but cant be sure thats mold either


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mycolorado]
    #28414983 -

The right side of #1 has been the fastest growth by far. On 07/28 I took a wedge and put it to grain as a gamble. Today it looks like this.


I also transferred another piece of it to another plate where it continues to grow quickly but is still translucent like it is on the germ plate (and not easily photographable). Should I take another transfer from the germ plate or transfer the T1 to a T2?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Pandaskis]
    #28414987 -

I've never seen one part of a culture show concentric rings while the rest grows uniform. Especially not with a clear separation between the two.

Usually two myc cultures will merge, not show a clear gap between them.

All that being said, you're the tc and I've been on a hiatus for a few years. :shrug:


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #28414992 -

Fox, what are your highs and lows? Temps swing a lot from afternoon to early morning?


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

Extras: Filter Print Post Top
Re: AGAR ENVY! (Anything and All things agar!) [Re: tryptkaloids]
    #28414996 -

tryptkaloids said:
Fox, what are your highs and lows? Temps swing a lot from afternoon to early morning?


21°C at night and up to 26°C in the late afternoon. My apartment gets a lot of sun and is on the top floor and the A/C can't keep up so I night cycle it (make it really cold at night so it has a head start against the sun).


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Edited by Arctic_Fox (07/31/23 05:22 PM)

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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