Stipe-n Cap said:

AspectOfTheCreator said:

Whats the general consensus on what to do once the PC timer hits 2 hours? Turn off heat, then what? Remove jars as soon as pressure drops? Let them cool off inside the pc before removing? Ive been removing them asap, stripping the foil off, and wiping off the bit of water ontop the lid asap.

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Let the jars cool in the pc until the next day. The pc will be fine until you open it. There's no way to prevent moisture in an environment saturated with pressurized steam. SFDs are hydrophobic.

If you're experiencing widespread contamination it will be due to user error. Go back to the basics, create a checklist until you're familiar enough.

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Yuggoth said:

These jars were inoculated via G2G 6 days ago. Shake or wait?

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I see no problem in shaking

mushhead said:

Those plates look alright, a little tomentose in my opinion, but they don't look like mold and there's no bacteria I can see.
So lets say you make new jars of spawn, PC for 2hrs, allow it to cool over night, and innoc using your chosen plate.
Assuming that your prep method for your spawn and your PC goes well we now have clean jars ready for mycelia.
Using just Coir, pasteurized via bucket tek method, or even PC'd if you want, with no additives like verm or other nonsense as your substrate. 
Taking pics along the whole way, jars especially, so you can have documentation for diagnosis.

EDIT: I want to add that after you've done this, if you're still having issues, you need to go over your sterile technique and write down what you're doing and make changes to your process.
Most likely if you fail after trying the above it's the culture, something we're not seeing, and you should perhaps start from spore.

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AspectOfTheCreator said:

mushhead said:

Those plates look alright, a little tomentose in my opinion, but they don't look like mold and there's no bacteria I can see.
So lets say you make new jars of spawn, PC for 2hrs, allow it to cool over night, and innoc using your chosen plate.
Assuming that your prep method for your spawn and your PC goes well we now have clean jars ready for mycelia.
Using just Coir, pasteurized via bucket tek method, or even PC'd if you want, with no additives like verm or other nonsense as your substrate. 
Taking pics along the whole way, jars especially, so you can have documentation for diagnosis.

EDIT: I want to add that after you've done this, if you're still having issues, you need to go over your sterile technique and write down what you're doing and make changes to your process.
Most likely if you fail after trying the above it's the culture, something we're not seeing, and you should perhaps start from spore.

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They're tomentose because they're on 1% nutrient agar. I hadn't taken pictures of my spawn because it was all unremarkable so I saw no reason to. I'm going to change to canna coir and leave the jars in the PC to cool. Started new plates, fresh start on everything. Will document everything along the way.

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mushhead said:

AspectOfTheCreator said:

mushhead said:

Those plates look alright, a little tomentose in my opinion, but they don't look like mold and there's no bacteria I can see.
So lets say you make new jars of spawn, PC for 2hrs, allow it to cool over night, and innoc using your chosen plate.
Assuming that your prep method for your spawn and your PC goes well we now have clean jars ready for mycelia.
Using just Coir, pasteurized via bucket tek method, or even PC'd if you want, with no additives like verm or other nonsense as your substrate. 
Taking pics along the whole way, jars especially, so you can have documentation for diagnosis.

EDIT: I want to add that after you've done this, if you're still having issues, you need to go over your sterile technique and write down what you're doing and make changes to your process.
Most likely if you fail after trying the above it's the culture, something we're not seeing, and you should perhaps start from spore.

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They're tomentose because they're on 1% nutrient agar. I hadn't taken pictures of my spawn because it was all unremarkable so I saw no reason to. I'm going to change to canna coir and leave the jars in the PC to cool. Started new plates, fresh start on everything. Will document everything along the way.

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Good show! Honestly PASTEURIZED COIR of ANY KIND as long as it's PASTEURIZED will work perfectly.
Make a grow log that you can share with us here that you can update weekly or whatever, this way you can have a time line and advice can be given.

EDIT: I want to add that I know others get away with just using regular water without a boil or pasteurizing it.
If you think your coir is the culprit, pasteurize/sterilize it before you change brands and waste coir/money.

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AspectOfTheCreator said:

Trich, turns blue, refuses to fruit, whatever the hell this is




I know people will refute it but I think its the coir. Theres saplings growing out of half the tubs I do, and despite every tek on here suggesting to use 5x the mass of water to coir to hydrate it, or to use 4-4.5 qts water to 1 brick and 2 quarts verm, this coir will only hold about 1/2 that, with or without verm, before being way over field capacity.

Im kind of grasping at straws looking for anything different I can be doing. My agar is always good and when its not its very obvious. So i dont think its my sterile technique.

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