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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Mycodemus] * 2
    #28346544 -

If the myc is beading then I'd hold off misting for a while. Just make sure that there isn't too much air, so that the substrate surface isn't drying out too quickly.


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Re: Ask Quick Questions, Get Quick Answers [Re: johnukguy]
    #28346546 -

johnukguy said:
If the myc is beading then I'd hold off misting for a while. Just make sure that there isn't too much air, so that the substrate surface isn't drying out too quickly.


I have the lid laying on top of the 2 closed latches, covered with a muslin cloth tied on top to keep out gnats. I can adjust it so its one latch on top and one beneath the lid.


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An hour and a half later, I was beginning to feel definite effects
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Re: Ask Quick Questions, Get Quick Answers [Re: Mycodemus] * 2
    #28346549 -

Mycodemus said:
johnukguy said:
If the myc is beading then I'd hold off misting for a while. Just make sure that there isn't too much air, so that the substrate surface isn't drying out too quickly.


I have the lid laying on top of the 2 closed latches, covered with a muslin cloth tied on top to keep out gnats. I can adjust it so its one latch on top and one beneath the lid.


Careful with that. Could end out drying out one side of your tub. If it is a true shoebox you can just leave the lid on normal and unlatched and you will get more than enough fae.


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Re: Ask Quick Questions, Get Quick Answers [Re: Way] * 2
    #28346551 -

Way said:
Mycodemus said:
johnukguy said:
If the myc is beading then I'd hold off misting for a while. Just make sure that there isn't too much air, so that the substrate surface isn't drying out too quickly.


I have the lid laying on top of the 2 closed latches, covered with a muslin cloth tied on top to keep out gnats. I can adjust it so its one latch on top and one beneath the lid.


Careful with that. Could end out drying out one side of your tub. If it is a true shoebox you can just leave the lid on normal and unlatched and you will get more than enough fae.


Done, thanks


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An hour and a half later, I was beginning to feel definite effects
Subjective Effect Index: comprehensive map of all potential psychoactive experiences

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Re: Ask Quick Questions, Get Quick Answers [Re: Mycodemus] * 3
    #28346597 -

One thing I'd add about the muslin cloth for gnats is to spray it with something like Microbhan (as it's called in the US).
It's an antifungal disinfectant that keeps cloth from going moldy if sprayed once a week. So long at it's not in contact with the mycelium it should be fine.
I tried with cheesecloth over a tub, to protect against gnats and it went moldy without being sprayed.

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Re: Ask Quick Questions, Get Quick Answers [Re: CocaineBuffet]
    #28346646 -

CocaineBuffet said:
Luftwaffles90 said:
First Lc, this is the what I'm seeing after testing it on agar. Put the lc onto the agar around 48 hours ago. Idk if it being this spikey is normal but I thought it kinda looked a little suspect






Looks very promising! :thumbup:


That's a relief. When I saw how spikey it was I thought it may have been some kind of mold or something. Thank you

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Re: Ask Quick Questions, Get Quick Answers [Re: johnukguy]
    #28346675 -

johnukguy said:
One thing I'd add about the muslin cloth for gnats is to spray it with something like Microbhan (as it's called in the US).
It's an antifungal disinfectant that keeps cloth from going moldy if sprayed once a week. So long at it's not in contact with the mycelium it should be fine.
I tried with cheesecloth over a tub, to protect against gnats and it went moldy without being sprayed.


It’s on top of the plastic lid, as I thought of that… so not in contact with any moisture or anything, but good tip!


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An hour and a half later, I was beginning to feel definite effects
Subjective Effect Index: comprehensive map of all potential psychoactive experiences

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Re: Ask Quick Questions, Get Quick Answers [Re: altford78]
    #28346763 -

altford78 said:
Way said:
altford78 said:
so what's the problem here then


Newbie thinks the outside of agar plates must be sterile at all times and would faint if he saw the conditions I keep my plates in.

:laugh:

I wiped my SAB out with alchohol before every use for so long I can't really judge.



there's a lid :unimpressed:

I wipe my sab with iso, dries fast and leaves no stains :fuckthepolice:

sprocket said:
Not sure, it sounds like they are being moved without the lids on. Not sure why you would do this.


:whoak:

I mean I've seen people take the lids off to take pictures of plates they intend to keep so nothing surprises me anymore



Thanks for the replies everyone, just want to clarify what's going on here.

The slime cups I'm using have lids.

I'm not moving them without the lids on.

I'm aware that the outside of the cups don't need to be sterile, as long as sterile technique in SAB is good and contaminants don't fall inside the cup itself during the work. 

That being said, my sterile technique is still a WIP and reducing the amt. of contaminants around the cups, whether by handling them less, sealing the unused cups in clingwrap, etc is my intention here.

But if you guys think it's pretty much fine, then that's a relief. & I'll continue to work on my sterile technique.

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Re: Ask Quick Questions, Get Quick Answers [Re: Mycodemus] * 2
    #28346771 -

Mycodemus said:
Second shoebox is starting to pin. How does it look? The myc is mostly beaded, but the coir is very dry in some exposed spots. Do I mist or no?




If your sub looks dry but you have good surface conditions, im assuming you didnt gave well hydrated coir at field capacity and that youre misting it to keep up surface conditions. In that case keep an eye on it to see if surface conditions are drying out and only mist then :smile:


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Re: Ask Quick Questions, Get Quick Answers [Re: Pandaskis]
    #28346828 -

Can I use hydrogen peroxide to kill contamination I find on substrate?

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Re: Ask Quick Questions, Get Quick Answers [Re: NuxFlegative] * 1
    #28346836 -

No - at best it will just hide whats actually growing in the substrate.

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome] * 1
    #28346838 -

Noticed that one of my BRF jars has a small mushroom growing along the side before I got to birthing it. 
Should I harvest it or just cover it with coir like the rest of the cake and wait for it to poke through?

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Re: Ask Quick Questions, Get Quick Answers [Re: PBJ710]
    #28346851 -

PBJ710 said:
No - at best it will just hide whats actually growing in the substrate.


What do you mean and how does it hide it?


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"Let yourself be silently drawn by the strange pull of what you really love. It will not lead you astray." – Divan-e-Shams

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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122]
    #28346858 -

The fruiting bodies of some hairy molds get knocked down by it, but the mycelium still survives in the substrate.  If you try to kill it, you will just kill any desired mycelium at the same time.

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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122]
    #28346862 -

Quote:
What do you mean and how does it hide it?



It might remove the visible contam on the surface but by the time the contam shows up on the surface the insides are long since contaminated so you'll just think you dealt with the problem but it will just come back in a few days even worse.

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Re: Ask Quick Questions, Get Quick Answers [Re: stroopwafelman]
    #28346872 -

stroopwafelman said:
Noticed that one of my BRF jars has a small mushroom growing along the side before I got to birthing it. 
Should I harvest it or just cover it with coir like the rest of the cake and wait for it to poke through?


You can continue to dunk and roll, some pins make it through the process, sometimes they don’t. The couple  that I had abort were swallowed up by mycelium and few days later got a nice cluster from that same area.


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Re: Ask Quick Questions, Get Quick Answers [Re: stroopwafelman]
    #28346877 -

stroopwafelman said:
Quote:
What do you mean and how does it hide it?



It might remove the visible contam on the surface but by the time the contam shows up on the surface the insides are long since contaminated so you'll just think you dealt with the problem but it will just come back in a few days even worse.


The H2O2 kills at the point of application. Of course it doesn't kill all throughout the substrate. What is the disadvantage?


--------------------
"Let yourself be silently drawn by the strange pull of what you really love. It will not lead you astray." – Divan-e-Shams

How to upload Photos

The Hitchhiker's Guide to the Shroomery

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Golden Teachers

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Re: Ask Quick Questions, Get Quick Answers [Re: Proposition 122] * 1
    #28346881 -

Quote:
Of course it doesn't kill all throughout the substrate. What is the disadvantage?



If you're seeing fruiting bodies of molds and such on the top of the substrate by this point the inside of the substrate is already overtaken by the contamination, so the disadvantage is just wasting time on a tub/cake because applying H2O2 won't remove the contam that's inside the substrate without destroying healthy mycelium that may or may not be left over at this point.

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Re: Ask Quick Questions, Get Quick Answers [Re: stroopwafelman]
    #28346961 -

In about a week I will be trying my first G2G transfer and I have a related dumb question.

What is the best way to transfer colonized grain to the sterile grain jar? Do I shake up the donor jar, and then pour in some grains? Do I use a sterilized tool, like a spoon? Is an iso-sanitized tool good 'nuff?


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We have not succeeded in answering all our problems. The answers we have found only serve to raise a whole set of new questions. In some ways we feel we are as confused as ever, but we believe we are confused on a higher level and about more important things. -- Earl C. Kelley

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Re: Ask Quick Questions, Get Quick Answers [Re: Nichrome]
    #28346963 -

Does this BRF jar look ok?
I'm a little concerned about pic 3 with the mycelium seemingly hugging the glass much more than anywhere else in that jar. Or is that normal?

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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