Nigel Stephens said:

Going to attempt growing out mycelium from 3+ year old dried, powdered and capsuled cubensis.

What do you think is the chance of success?

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rumfor69 said:

QQ: Do I use fortified or non fortified nutritional yeast?

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tholos said:

3) Pretty standard agar to grain, wait for the jar to colonize and then 1/4 - 1/5 of the grain per jar to noc a new myco quart

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Yuggoth said:

tholos said:

3) Pretty standard agar to grain, wait for the jar to colonize and then 1/4 - 1/5 of the grain per jar to noc a new myco quart

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If people inoculate with one agar wedge why do you need two cups of inoculant when doing G2G? If that much is required it makes the procedure much less attractive because your library jars aren't going to last long.

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Yuggoth said:

Thanks for that.

If you make a new master jar every time you do g2g jars, is there a problem with growing too many generations? Or is that not a concern?

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Pandaskis said:

My 2 jars that i was planning to make master grains went bacterial , obviously i wont be using thes for g2g... Do you think its too far gone to spawn to bulk? Should i top fruit these?

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thirdeyewild said:

Nigel Stephens said:

Going to attempt growing out mycelium from 3+ year old dried, powdered and capsuled cubensis.

What do you think is the chance of success?

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Someone did this not too long ago here. It worked but they were disappointed with the results (weak growth, high contamination rates). I think in the end the bought a new spore syringe.

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Prolific Koi said:

Inoculated same agar dish with left over MAZ and RW prints. Will I get a variation of the two or will they come out normal? Please and thank you.

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Quote:

If you want to cross two strains(I'm using 'strain' in the sense it's usually used here, that is naming a print and calling it a strain), simply print one cap right on top of the print left by another cap.  By then using the resultant print, it would be nearly impossible to not get several crosses.  You'd want to do it on agar and isolate the strains as they differentiate, carefully labeling each one.  when you fruit them, you'll be able to tell which is a cross or not, provided you used two distinctly different parents.
RR

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Pandaskis said:

Pandaskis said:

My 2 jars that i was planning to make master grains went bacterial , obviously i wont be using thes for g2g... Do you think its too far gone to spawn to bulk? Should i top fruit these?

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Shameless bump :3

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Pandaskis said:

Pandaskis said:

My 2 jars that i was planning to make master grains went bacterial , obviously i wont be using thes for g2g... Do you think its too far gone to spawn to bulk? Should i top fruit these?

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Shameless bump :3

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