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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: ruawakeyet] * 1
    #28160863 -

ruawakeyet said:
I think she was assuming you were using one-piece plastic lids. Those are commonly used and highly recommended over the two-piece metal lids.

With that being said, I really dislike using jars for agar, but they can work. Mainly, I just didn't like the steep angle it forces me to use for transfers. That makes it difficult to get the agar segment placed in the middle instead of stuck on the tip of the scalpel or knife.


Agreed. The walls make it hard to place the transfers and impossible to stack and fit many in a SAB. I find that after cutting a piece you can stab it then kind of wipe it on the new plate. It "usually" works. I'd like to use petri dishes but I'm somewhat of an environmentalist and I can't stomach the idea of creating all that waste. Personal problems, I know.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator]
    #28161005 -

I agree with the single use petri issue. I wanted to go with glass petris but the cost of quality ones prevents me from going that route. There are PP petris that can be sterilized in a PCer at 15 psi. My only attempt using no-pour in them was a little shaky, but I know others are having success with them.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ruawakeyet] * 1
    #28161050 -

Im doing pour agar. One interesting thing is this Watkins food color does not survive the PC cycle very well. It usually goes into the cycle brilliantly colored, and comes out barely colored.
https://www.amazon.com/Watkins-Food-Coloring/dp/B01E6OLE64/

My progression of recipes:

recipe 1:
400mL Water
7.2g agar agar
6.4g LME
1.6g dextrose

recipe 2:
400mL Water
4g agar agar
6.4g LME
1.6g dextrose
(notes: too soft)

recipe 3:
400mL
6.25g agar agar
6.4g LME
1.6 dextrose

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Re: AGAR ENVY! (Anything and All things agar!) [Re: shitakee] * 2
    #28161065 -

You could skip the dextrose and see if that clears up your agar. The ME by itself will grow everything you need it to grow. You might try skipping the food coloring also, if you want clear agar. That's probably the most likely culprit, more so than the dextrose.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: shitakee]
    #28161086 -

shitakee said:
Im doing pour agar. One interesting thing is this Watkins food color does not survive the PC cycle very well. It usually goes into the cycle brilliantly colored, and comes out barely colored.
https://www.amazon.com/Watkins-Food-Coloring/dp/B01E6OLE64/

My progression of recipes:

recipe 1:
400mL Water
7.2g agar agar
6.4g LME
1.6g dextrose

recipe 2:
400mL Water
4g agar agar
6.4g LME
1.6g dextrose
(notes: too soft)

recipe 3:
400mL
6.25g agar agar
6.4g LME
1.6 dextrose


"organic" food coloring doesn't survive the temps in the pc. Just use regular ol food coloring.


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: AspectOfTheCreator]
    #28161191 -

Quote:
AspectOfTheCreator said: I'd like to use petri dishes but I'm somewhat of an environmentalist and I can't stomach the idea of creating all that waste. Personal problems, I know.


I felt the same way. In my first 8 months I threw out close to 500 plates. I since went to this tek and use the plates he recommends. They are I slight bit harder to see through but I think it's a good trade off. I have 60 of them and keep them in rotation of about 30-40 per PC cycle. I've also put about 85% of my cultures in long term water storage to reduce the plates needed. I recently was pointed in the direction of condiment cups that have pp5 lids with the cup that I just ordered. I don't know how they are yet. Here is the holy grail pp5 petri Tek and here is the link for the pp5 cups I just ordered.

Edited by Bigworm (01/28/23 11:21 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bigworm] * 2
    #28161204 -

why are y'all writing in bold?


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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho] * 4
    #28161263 -

Stone-producer myc is always so funky.
I love the color change, it's especially visible from the bottom.


Edit:
Pardon the slow growth, it's been so cold here lately.
Interior temps getting down to the 50s in this shitty cabin...

Edited by Greg (01/28/23 12:15 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Greg]
    #28161332 -

Here’s a picture of year old  gt lc started from a Joesex poke passing the blizzard challenge, it fell a split second after the picture was taken… 

Edited by Enjoil (01/28/23 01:20 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Enjoil] * 1
    #28161600 -



Is this a jack frost trait?
Never had a germination plate looking like this.
Even the T1 is thick and aerial overlayed...


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β€œAll this buttoning and unbuttoning”

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 999999999]
    #28161806 -

Gleyck said:


Is this a jack frost trait?
Never had a germination plate looking like this.
Even the T1 is thick and aerial overlayed...


My JF was super puffy like that, then I lost it...

:super:


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Mushroom Cultivation Techniques...

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Grogu]
    #28161826 -

fell off yo pocket?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: 999999999] * 1
    #28161839 -

Gleyck said:
fell off yo pocket?


Dog ate it...

It was my homework...

:lol:


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Mushroom Cultivation Techniques...

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Re: AGAR ENVY! (Anything and All things agar!) *DELETED* [Re: 999999999] * 1
    #28162010 -

Post deleted by Phaedra

Reason for deletion: X

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Phaedra] * 3
    #28162510 -


Some AA+ T2 plates from fresh start with syringe. These are one week after transfer i dont know if thatΒ΄s slow or fast. They look fine to my untrained eye, which leaves me to believe my issues lie in grain prep or inoculation. I have yet to put this AA on grain, i hope i get better results compared to my unsuccessfull KSSS grows.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: dr.snuggles] * 5
    #28162511 -

Some Pan cyan I'm putting to grain today



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:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ruawakeyet] * 2
    #28162524 -

ruawakeyet said:
Those don't even come with silicone gaskets, at least not the box I bought.


I use those brown lids, wide mouth version, no gasket supplied or required. I think they are amazing, you can drill into them and screw in a syringe filter if you like, they will also accept a self sealing syringe port. I drill one 1/4" hole and rtv sealant a 1 1/4" diameter sfd overtop. Lids seal up great and after 3 years of PCing I haven't lost a lid yet.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: xVandalx] * 1
    #28162603 -



OMNI pining in slants I never got around to putting in the fridge


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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Re: AGAR ENVY! (Anything and All things agar!) [Re: SirPsycho]
    #28162605 -

Are those stored in distilled water? Seems like a good culture :3


--------------------
I am not crazy, I prefer the term mentally hillarious.

Agar - The way to go!
Clean spawn checklist
Proper Surface Moisture
Recognizing and dealing with contamination
πŸ…ƒ πŸ„΄ πŸ„° πŸ„Ό    πŸ„² πŸ„» πŸ„Έ πŸ„½ πŸ„Ά πŸ…† πŸ… πŸ„° πŸ„Ώ

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Shrimps] * 1
    #28162612 -

Shrimps said:
Are those stored in distilled water? Seems like a good culture :3


No, just agar and a piece of wood. It's an enigma type mutation off Melmak TP. Extremely aggressive fruiter. It's actually one of the pics in my sig


--------------------
:pm:Ask about free prints: Ps tampanesis, subtropicalis, cubensis(ESS) and Pan cyanescens:pm:
Balance in life is like running on ice.

  πŸ…‘πŸ…žπŸ…£πŸ…£πŸ…›πŸ…” πŸ…–πŸ…πŸ…πŸ…–

"Mist your balls and fan your asshole" - Pandaskis, 2023

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