bigfootscreepyuncl said:

certainly looks like it has potential. Let it grow out a little further and you'll have a better idea in a few days.

Best to learn patience now. Everything in this hobby is a few days away, a couple weeks until 'whatever'...unless you get busy and put off harvesting for another day then your entire crop is black with spores

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haha yeah, been there. admittedly it's been over a decade since I've had a monotub blow its load due to college era shenanigans.

after waiting two weeks for the first round of plates to do nothing with one being confirmed contaminated, and now this batch taking five days to mostly do nothing, i'm kinda lacking in that dept now haha. BUT with confirmed "good" growth, i won't open the incubator for a few days and let it do it's thing uninterrupted.

even if this round ends in 100% contamination, success has been achieved! germination on agar is possible for a rusty old man like me. now the real work can begin

ruawakeyet said:

Now that I can see it better, I wasn't even close. Rusty has better eyes than I do! Anyway, looking good!

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thehiveatp said:

GERMINATION Y'ALL!!!

Sorry for the excitement, my kids don't give a shit about microbiology yet, and I don't share this hobby with anyone else in my life, so you all have the privilege of dealing with my excitement here.

A couple lessons learned, I think.

First, I'm pretty sure my agar is too hard, that's why the first five plates I tried anything with did nothing. Did 10g agar, 10mg LME, 500ml and pre-boiled before PCing (learned on this thread that last step was a poor idea).

Second, pre-stretching the parafilm seems to be SOP. The parafilm has split on a handful of plates, so will be trying the pre-stretch next time.

Will also be trying 7g agar instead of 10g to 500ml water, see if softer agar works out better for germination.

Otherwise, here is a pic of an APE multispore plate I knocked up this past weekend. Of the three plates I hit with these spores, this is the only one that is showing signs of life. Also did three with psilocybe cyanescens, and three with pan cambo, and none of those have shown any signs of doing anything.



Just wanted to celebrate here a little and say thank you to all you fine people that have posted so much information, lessons learned, etc for decades. It's much appreciated! I'm working on a setup for better plate pics as well, so promise I'll quit dropping these bad ones here soon

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thehiveatp said:

GERMINATION Y'ALL!!!

Sorry for the excitement, my kids don't give a shit about microbiology yet, and I don't share this hobby with anyone else in my life, so you all have the privilege of dealing with my excitement here.

A couple lessons learned, I think.

First, I'm pretty sure my agar is too hard, that's why the first five plates I tried anything with did nothing. Did 10g agar, 10mg LME, 500ml and pre-boiled before PCing (learned on this thread that last step was a poor idea).

Second, pre-stretching the parafilm seems to be SOP. The parafilm has split on a handful of plates, so will be trying the pre-stretch next time.

Will also be trying 7g agar instead of 10g to 500ml water, see if softer agar works out better for germination.

Otherwise, here is a pic of an APE multispore plate I knocked up this past weekend. Of the three plates I hit with these spores, this is the only one that is showing signs of life. Also did three with psilocybe cyanescens, and three with pan cambo, and none of those have shown any signs of doing anything.



Just wanted to celebrate here a little and say thank you to all you fine people that have posted so much information, lessons learned, etc for decades. It's much appreciated! I'm working on a setup for better plate pics as well, so promise I'll quit dropping these bad ones here soon

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Screwup said:

thehiveatp said:

GERMINATION Y'ALL!!!

Sorry for the excitement, my kids don't give a shit about microbiology yet, and I don't share this hobby with anyone else in my life, so you all have the privilege of dealing with my excitement here.

A couple lessons learned, I think.

First, I'm pretty sure my agar is too hard, that's why the first five plates I tried anything with did nothing. Did 10g agar, 10mg LME, 500ml and pre-boiled before PCing (learned on this thread that last step was a poor idea).

Second, pre-stretching the parafilm seems to be SOP. The parafilm has split on a handful of plates, so will be trying the pre-stretch next time.

Will also be trying 7g agar instead of 10g to 500ml water, see if softer agar works out better for germination.

Otherwise, here is a pic of an APE multispore plate I knocked up this past weekend. Of the three plates I hit with these spores, this is the only one that is showing signs of life. Also did three with psilocybe cyanescens, and three with pan cambo, and none of those have shown any signs of doing anything.



Just wanted to celebrate here a little and say thank you to all you fine people that have posted so much information, lessons learned, etc for decades. It's much appreciated! I'm working on a setup for better plate pics as well, so promise I'll quit dropping these bad ones here soon

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I’m like pretty sure that’s all bacteria or bacteria mostly barely mixed with mycelium. Kinda hard to tell and I may be wrong.

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Go drink a coffee ☕, check his second pic in a later post and then wear the shame cube

ruawakeyet said:

That shame cube could have been assigned to me last night...

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Not quite he had not posted the second pic at that point. Plus Screwup fits just perfectly in a cube

ruawakeyet said:

That shame cube could have been assigned to me last night...

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Pretty sure that I see Rusty sitting with a shame cube on his head more than anyone else

Rotnpins said:

ruawakeyet said:

That shame cube could have been assigned to me last night...

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Pretty sure that I see Rusty sitting with a shame cube on his head more than anyone else

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That's not a cube

SirPsycho said:

Rotnpins said:

ruawakeyet said:

That shame cube could have been assigned to me last night...

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Pretty sure that I see Rusty sitting with a shame cube on his head more than anyone else

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That's not a cube

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Shrimps said:

In my time in a Lab, I learned that Agar wont get boiled. Wen it gets boiled in the lab, they toss it. It's  enough when heating it on the stove until alot of steam rises but not yet boiling. You notice that at some point, the liquid starts to foam, that's when all the nutes and agar got dissolved. Then I fill it in a bottle to PC for 20min.

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Mushroplex said:

Great stuff mate
For my egg or recipe I used 150 mL of water then I use 10 G of a car 1 G of yeast 7 G of malt extract some color dye and cooked then I put it in the PCI for 90 minutes

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ruawakeyet said:

Mushroplex said:

Great stuff mate
For my egg or recipe I used 150 mL of water then I use 10 G of a car 1 G of yeast 7 G of malt extract some color dye and cooked then I put it in the PCI for 90 minutes

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How hard did that agar turn out? And isn't that pretty rich on the nutes? And if you're only PCing agar, 20 minutes at 15 psi should be enough, right everyone?

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I didn't bother answering I thought the person was trolling