LtLurker said:

Smellyhobbit said:

Quick question: is there value in taking tissue samples from a sparse but THICC second flush fruit? Each of my golden teacher tubs are throwing a typical second flush, but in the same spot in each tub there’s an absolute unit growing.

Now I know if I cloned it they wouldn’t all be that large. Just wondering if you guys normally take from flush one, or if it’s more of a “whatever looks best” situation.

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Big fruits are easier to get a clean sample from than thin ones and I try to select fast ones from the first flush by keeping an eye on it while it matures. Sometimes the results are similar, sometimes they aren't. I think you don't really know what to expect from a clone for size & clusters till you run it, but is better at increasing the odds of recessive traits and mutations you come across.

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BobRotten said:

After the first flush has finished, should I wait a while before starting the second?

Cheers

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The second flush will happen on its own.. all you have to do it maintain surface conditions

Rusty2096 said:

What does F stands for? I feel like someone people use it do describe how many generations away from the wild and others for the number of flushes. Thanks

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itskater said:

Thanks everyone!
Hygrometer out and shut the lid for a few days.

About how many days should I wait before checking on it?
I wanna do it as least as possible.

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check back in a week

CocaineBuffet said:

You ordered a colonized cake? If you inoculated a pre-sterilized cake I don't think a vendor would reimburse you because you would not be able to prove that it was their product that was faulty or that you (most likely) were the reason it became contaminated.

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shroomsurf said:

Is there a better brand for media bottle, some posters said they cracked at 15psi...

https://www.amazon.com/gp/product/B07WSS8LS5

Thanks,

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Yo Shroomsurf I've used those ones and the pyrex version. I honestly like the pyrex alot more. I did crack one of the cheap ones a while back but I don't remember how I managed to crack it I could have just dropped it or something its been awhile.

I have these coming now
https://www.amazon.com/Pyrex-Slimline-Media-Bottle-Corning/dp/B00QU9BVH6/ref=mp_s_a_1_6?crid=3UE7DY429ARD1&keywords=pyrex+slim+line&qid=1662959580&sprefix=pyrex+slim+line%2Caps%2C784&sr=8-6

Couldn't tell you how they compare to the others yet but they look cool.

Grind365 said:

shroomsurf said:

Is there a better brand for media bottle, some posters said they cracked at 15psi...

https://www.amazon.com/gp/product/B07WSS8LS5

Thanks,

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Yo Shroomsurf I've used those ones and the pyrex version. I honestly like the pyrex alot more. I did crack one of the cheap ones a while back but I don't remember how I managed to crack it I could have just dropped it or something its been awhile.

I have these coming now
https://www.amazon.com/Pyrex-Slimline-Media-Bottle-Corning/dp/B00QU9BVH6/ref=mp_s_a_1_6?crid=3UE7DY429ARD1&keywords=pyrex+slim+line&qid=1662959580&sprefix=pyrex+slim+line%2Caps%2C784&sr=8-6

Couldn't tell you how they compare to the others yet but they look cool.

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I bought these cause the video was awesome and they look cool ...lol

https://www.amazon.com/gp/product/B092MBJSLQ

Screwup said:

Why does it matter what contam? The big thing is knowing you’ve got contam…and ya do. Clean up your sterile procedure or it’s the syringes you used.

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DERRAYLD said:

It's a syringe, his jars were prepared correctly.

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chuparosa said:

How do I sterilize used syringes in a pressure cooker?

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