rumfor69 said:

No no lol I meant the quote code is has me as "rumfor69 said" with that green fetus dish

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Smellyhobbit said:

Green Fetus Dish is my new math rock band name

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manlord said:

Hey guys any recommendations for getting away from bacteria on agar? I've got 1 strain to work with rn and it's def got some hidden bacteria. I can even see a tiny "droplet" of bacteria on the original spore plate. The recent xfers look better, but still seeing signs of bacteria.

I've tried hot pours, bacteria grew straight through the LME+gentamicin agar? Thought bacteria couldn't grow THROUGH agar, am I wrong? Also did a couple Josex BRF Poke things. I feel like those look better than my agar-agar xfers, but still contamed.

How do you get sneaky, hitchhiking bacteria to f off? Should I try more pokes, hot pours/sandwiches, and just quickly xfer from best looking edges? What's your preferred method, I'm really trying to get something clean to run rn. I have nothing atm

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Rotnpins said:

I'm in beast mode tonight.. idk why the hell I waited so long to get my FFU.. it doesn't feel like such a chore to do things like pour plates..


Started some Gandalf, Semperviva, and Hillbilly germ plates.



@guerrilla, yup my plates come in sleeves of 10, it's pretty convenient.

Yeah, I know the plate won't be necessary.. more of a luxury item.. I'll probably use it for both LC and agar

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Yeah when you was talking about often not having a cooling plate handy in a SAB, I was actually going to mention that I really doubt you will have that issue when you have the FFU, as you can pour sooo much more and much easier. I always have countless plates at hand!

Ohh, I was gonna say, I thought you were separating 20 stacks. 10 sleeves would be annoying for me in big pours personally, but as long as they work for you.

But a stir plate in agar really is pointless, LC I understand, but it's not something that people do for agar, the agar is still going to cool down regardless, and you have a more than reasonable working window so it should not be an issue anyway. The only real reason a stir plate would be implemented in agar is if you had a heated stir plate because you were unable to complete a pouring session in quick succession, for whatever reason. Though I've not once had this issue and doubt you would either. :p

23:57 PC put on heat
00:16 steam begins
00:31 weight added
00:38 psi up to 17 then heat on lowest
00:58 heat killed
01:31 pressure dropped
02:33-02:37 begin pour
02:57 100 plates poured
03:27 plates set

Your times may differ according to how quick you are, your pour temp and your room temp, however, as you can see you have plenty of time for your pour, and when you time your own pours as such, you know exactly when you need to start, and when you have free time to get on with other things, so a stir plate never needs to be in the equation.


EDIT:

Just wanted to add, if you wasnt totally ready to pour after pulling from the PC, a water bath would suffice.

manlord said:

Hey guys any recommendations for getting away from bacteria on agar? I've got 1 strain to work with rn and it's def got some hidden bacteria. I can even see a tiny "droplet" of bacteria on the original spore plate. The recent xfers look better, but still seeing signs of bacteria.

I've tried hot pours, bacteria grew straight through the LME+gentamicin agar? Thought bacteria couldn't grow THROUGH agar, am I wrong? Also did a couple Josex BRF Poke things. I feel like those look better than my agar-agar xfers, but still contamed.

How do you get sneaky, hitchhiking bacteria to f off? Should I try more pokes, hot pours/sandwiches, and just quickly xfer from best looking edges? What's your preferred method, I'm really trying to get something clean to run rn. I have nothing atm

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rumfor69 said:

manlord said:

Hey guys any recommendations for getting away from bacteria on agar? I've got 1 strain to work with rn and it's def got some hidden bacteria. I can even see a tiny "droplet" of bacteria on the original spore plate. The recent xfers look better, but still seeing signs of bacteria.

I've tried hot pours, bacteria grew straight through the LME+gentamicin agar? Thought bacteria couldn't grow THROUGH agar, am I wrong? Also did a couple Josex BRF Poke things. I feel like those look better than my agar-agar xfers, but still contamed.

How do you get sneaky, hitchhiking bacteria to f off? Should I try more pokes, hot pours/sandwiches, and just quickly xfer from best looking edges? What's your preferred method, I'm really trying to get something clean to run rn. I have nothing atm

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Some ppl have luck with the sandwich method. Making tiny transfers also helps by using a syringe you flame the needle and when its cooled just try to scrape a tiny piece of mycelium to transfer. Or do this same thing with your scalpel. Dont forget to flame sterilize. You only need a tiny filament of mycelium for it to grow. Good luck!

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Rotnpins said:

rumfor69 said:

manlord said:

Hey guys any recommendations for getting away from bacteria on agar? I've got 1 strain to work with rn and it's def got some hidden bacteria. I can even see a tiny "droplet" of bacteria on the original spore plate. The recent xfers look better, but still seeing signs of bacteria.

I've tried hot pours, bacteria grew straight through the LME+gentamicin agar? Thought bacteria couldn't grow THROUGH agar, am I wrong? Also did a couple Josex BRF Poke things. I feel like those look better than my agar-agar xfers, but still contamed.

How do you get sneaky, hitchhiking bacteria to f off? Should I try more pokes, hot pours/sandwiches, and just quickly xfer from best looking edges? What's your preferred method, I'm really trying to get something clean to run rn. I have nothing atm

---

Some ppl have luck with the sandwich method. Making tiny transfers also helps by using a syringe you flame the needle and when its cooled just try to scrape a tiny piece of mycelium to transfer. Or do this same thing with your scalpel. Dont forget to flame sterilize. You only need a tiny filament of mycelium for it to grow. Good luck!

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Water agar also helps a lot

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