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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nimpo] * 2
    #27853954 -

Nimpo said:
How long do you guys wait for a agar culture that’s been in the fridge for a few months to warm up and be ready for transfers and sending to grain?


0 seconds

Just send it

With that logic it's gonna "warm up" on the new plate or jar anyways lol :wink:


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Being pissed on does not make you a real man.

...OR DOES IT?

Edited by Guerrilla (07/07/22 08:59 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Guerrilla] * 1
    #27854005 -

I’ll admit I’m a noob, but 0 seconds worked for me multiple times. Both A2A and A2G. I have waited 24 hours to let grain jars warm back up before going to bulk, but I’m skeptical that’s necessary.


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Tweeq’s wood lovers | Recipes | Moopers nats notes | Moopers subtrop notes | Milkboy gummies

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Re: AGAR ENVY! (Anything and All things agar!) [Re: BlueAndOrange]
    #27854008 -

I take a transfer from fridge plates and let that grow out before transferring out to LC.  I've been burned by going straight to LC/grains a couple times when I didn't take the extra transfer.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PBJ710] * 1
    #27854126 -

What does an organism have to gain from 24 hours in one suitable environment vs 24 hours in an environment that’s also perfectly suitable?

That’s like transporting a person from a beach while they’re sleeping to another beach across the world. When they wake up, it’s slightly different and they might have to use different tools but they’ll at least walk around.


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Mehhh



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Re: AGAR ENVY! (Anything and All things agar!) [Re: Wall.E] * 4
    #27854210 -



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Crack's Tek's and Shit


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa] * 1
    #27854227 -

I send fridge plates without waiting, no point in storing them if I thought they would be dirty coming out.

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Re: AGAR ENVY! (Anything and All things agar!) *DELETED* [Re: Tormato] * 1
    #27854303 -

Post deleted by Oneleggedwarrior

Reason for deletion: ...

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Oneleggedwarrior]
    #27854892 -

If you're gonna attempt a transfer do it now
where it's the furthest away from a satellite
growth, and take a very small sample if possible


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Links For Beginners And Beyond:mspoil:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rumfor69]
    #27854906 -

I prepped 7 grain jars last night and inoculated them this morning with these FP+ plates. I could not see any contams, but plates grew out in basically two different ways as pictured. I don't know what made the difference--genetics, invisible contamination, or what? Anyway, I'm just pushing forward with what I've got and hoping for the best. They seem pretty healthy to me anyway.







Here's all my FP+ plates just prior to transfer.



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Edited by Not Quite Social (07/08/22 04:15 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Not Quite Social]
    #27854945 -

Not Quite Social said:
I prepped 7 grain jars last night and inoculated them this morning with these FP+ plates. I could not see any contams, but plates grew out in basically two different ways as pictured. I don't know what made the difference--genetics, invisible contamination, or what? Anyway, I'm just pushing forward with what I've got and hoping for the best. They seem pretty healthy to me anyway.







Here's all my FP+ plates just prior to transfer.




Looking good! Hella tome! I'm waiting for my Fp+ to germ now :mushroom2:


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You're conscious population 2 stardust
----------------------
Nothing matters so everything does :sunny:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: cozmyc]
    #27855035 -

Please forgive the atrocious pics, camera's busted. But I'm just trying to figure out if I should be taking transfers from these germ plates at this stage?



The second plate germ'd first, but there are flecks of contamination starting to encroach on its margins. The first plate seems to be totally clean, fingers crossed.

Do I let em grow out more, or do I start doing transfers?

First timer at any kind of cultivation aside from propagating cyan and azure myc


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Edited by Spank (07/08/22 06:09 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Spank]
    #27855051 -

If the plate looks clean, I'd let it grow a bit.. but you may want to wait for someone with more experience to reply


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Wire-Lock Challenge Grow Log

Spore to Grain
H2O Agar
Plate Pins: Clone & Test at the same time
Updated PF Tek
H2O tub
Signs of contamination
Agar Transfers
Surface Conditions

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins]
    #27855069 -

Is the one with contams already sporulating?  If so, I wouldn't use any cultures from it since you have a healthy one available.  You can transfer any time after you have clean growth away from the inoculation point.  Letting it go longer makes it easier to find the faster cultures, but you don't want them to get overgrown where you can't tell what you are transferring.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Rotnpins] * 1
    #27855080 -

I honestly don't know if the contams are sporulating, as I'm not sure what they are and they wouldn't show up on my camera. It's some kind of film that is slowly growing little colorless specks that show up under bright light fairly evenly throughout. One spot is larger, not sure if it's a different species, but it is maybe turning yellowish in the center.

I'm not too worried about a transfer contaminating as I have lots of agar, rather I'm just trying to move things along as fast as I can, and have the eggs spread out in many baskets xD

On that second plate, the protruding myc is farthest from contams. I'm wondering if I should transfer asap to avoid bringing along unwanted visitors.

I'm pretty sure the contam was spread around by heavy condensation, so there is quite a bit, especially in spots where water pooled.

Mainly I just want to make sure this is viable for transfers, or if I might fuck things up by jumping the gun. Also, there's like zero chance that this is monokaryotic, right?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Spank]
    #27855130 -

How do these look? Should I pull a transfer or let them grow out a bit more. Do they look clean? If I did transfer, on the 1st plate I'm not sure of where to take from. For the 2nd I was thinking 3 & 9 o'clock but please correct me if I'm thinking wrong.



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Pastywhyte’s Ez dialed monotubs
My Miraculix Potency Test Results
The Hitchhiker's Guide to the Shroomery
My Museum
REV'S Tested: Dehydration temperature vs potency





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Re: AGAR ENVY! (Anything and All things agar!) [Re: cozmyc]
    #27855221 -

"Hella tome" :grin: ........yep

Thanks for the encouragement and good luck with your FP+. Mine germinated fast and contam-free. Praise Faht, the man sends clean swabs!

*

*

*

Can anyone tell me if the way this myc grew out is acceptable or bad, and why? Thanks



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Re: AGAR ENVY! (Anything and All things agar!) [Re: Not Quite Social] * 1
    #27855255 -

Depends who you ask from what I’ve seen on here.  That area appears to be colonized but with very thin myc.  Nichrome says it’s just sectoring and under a microscope it appears to be myc there. 

Id imagine it’s not great to have a bunch of that myc in your grain jars tho.  Since it colonizes so thinly, maybe it’s not great to have that myc on a lot of grain kernels as it doesn’t provide very much protection against an aggressive mold spores/mycelium. 

If it was bacteria, it’d grow like bacteria and look like a booger but that’d be a cool project for someone to figure out definitively

But I’m just speculating.  Maybe someone that has experience with sending plates like that can chime in

Not Quite Social said:
Praise Faht, the man sends clean swabs!



:headbanger:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster] * 1
    #27855267 -

I've never sent one myself because it "doesn't look right," but love to see what it really does.


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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Not Quite Social] * 3
    #27855269 -

Bigworm said:
How do these look? 



i like em. id grow em out a bit maybe double in size see how they look. but good so far.

Not Quite Social said:




i always trash those cause i thought they looked like ass and the few times i used em i molded but maybe it was some other issue:shrug:


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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: AGAR ENVY! (Anything and All things agar!) [Re: fahtster]
    #27855284 -

Ah, thank you very much Faht. That's reassuring. I searched Nichrome's posts and found it right away :thumbup:  So this might just be sectoring due to genetics. I hope so. We'll see....

*

Thanks for the feedback Sandman & Mushboy, too. I will track this plate to grain and to harvest and we will see how my grain jars and tubs eventually turn out compared to jars and tubs that started out from plates with more uniform growth. So, now I have a plan anyway :smile:

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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