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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Wes377]
    #27670693 -

nektar how did that culture preform?


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- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sandman420]
    #27670891 -

sandman420 said:
nektar how did that culture preform?


Never put it to grain. Still in the fridge. I got busy with other things. haha.

I'm going to do some pints, instead of quarts, to test some things soon, maybe I'll do a Z Strain.


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

Edited by nektar61 (02/23/22 10:50 PM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sandman420]
    #27670901 -

Sand would you suggest I should send any of the plates I posted earlier to grain?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: manlord]
    #27670921 -

R-->L APEU | McK | REISHI

APEU josex poke toi LC and tossed
McK  josex poke toi LC and to 3 jars of millet
REI  josex poke toi LC and to 4 jars of millet



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SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: manlord]
    #27670936 -

manlord said:
Does this look OK for grain?  (light colored spots are light reflections, not bact)



I put it on some antibiotic agar bc I've been dealing with bacterial contam.

It looked like this on some BRF agar with gentamycin:





I xfered to some new (non antibiotic) BRF agar, I think this one was higher nute %:



Wasn't liking that clear growth so I tried it on some PDA and it looks like this so far:




Thoughts? Any of these plates looking ready for grain?



(Side-note): What's up with these warm pours I did? Is this even myc?




I would say the top one if I was in a hurry, only a slice from the outer part. Since it's on Antibiotic agar I would transfer to Non first. Shit can still ride along. The plate farther down on PDA may be good, hard to tell. The hot pour plates still have something riding along.


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Crack's Tek's and Shit


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Crackatoa] * 2
    #27670959 -

Noccd up a quart of wbs with this Jack Frost for LAGM.

I cut it like a pizza and tiger dropped it in. 


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Vibetyme's LAGM 2.022

Vibetyme's LAGM_23

Vibetyme's LAGM_24

                 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Vibetyme]
    #27671045 -

noice


--------------------
SpaceBaby
SPACEBABY'S LAGM22 THREAD
MUSHBOY'S SHROOM TEA TEK

Me as a cube

Another Day's Work:


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Re: AGAR ENVY! (Anything and All things agar!) [Re: SpaceBaby] * 1
    #27671055 -

I've been struggling with these ESS swabs for a year now with nothing but bacteria.
I gave it a final stab and got my remaining swab to germinate with bacteria everywhere.
A small piece was transferred which is also bacterial but it has some clean mycelium, it's a good exercise but it's going to take a while.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: sandman420]
    #27671144 -

sandman420 said:
nektar how did that culture preform?


OK, Wes377 showing Z inspired me. I pulled mine out of cold storage (from 6 months ago) and put to new agar, will put to grain if it performs well.

While I was doing that I also pulled some Alacabenzi and Orissa  T3 mycelium on agar from the fridge that never went to grain, and put to plates.

Also, I found a big food prep tub, like would be used to make salad in a cafeteria, at Salvation Army. It's about 3 inches high. I'm using it upside down as a stand in front of my flowhood, to get my work up into the sweet spot. Is shown in bottom photo:





--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #27671255 -


First time with Agar please take it easy on me 🥴
Yes That tape is micropore tape and yes Somebody has let me know that’s a no no

Can somebody help me identify if that picture is of healthy mycelium
And also does keeping that contaminated plate around put me or the rest of the plates at risk

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bonerjamz] * 2
    #27671281 -

That looks like mold, it's important you focus your streaking to predefined zones so that you know if it's mold or not.
Streak a z or put your spores in the center, that way you can identify satellite contamination.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD]
    #27671323 -

Thank you for the information

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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD] * 1
    #27671336 -

Here's the subsequent transfer, going to take a while to get this clean.



Finally have some clean TAT and and Pearly Gates.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: DERRAYLD] * 1
    #27671360 -

Nat
:cheers: A.k.a

Edited by labbar (02/24/22 10:42 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #27671410 -

nektar61 said:
Also, I found a big food prep tub, like would be used to make salad in a cafeteria, at Salvation Army. It's about 3 inches high. I'm using it upside down as a stand in front of my flowhood, to get my work up into the sweet spot. Is shown in bottom photo:




I need to go get some cookie cooling racks like those.  Restaurant supply stores usually have large sizes cheap.  I've been using close-spaced wire closet shelving, which works OK but I keep dropping my scalpel through it.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Shroomintune]
    #27671733 -

I made some no pour plates, and a drop of condensation dropped from the top of the lid onto the agar. Being that everything inside (including the condensation) is sterile, would it be okay to use my swab on this agar mixed with the condensation droplet? Or should I use a totally dry plate?

I'm thinking since MS syringes are spores mixed with sterile water, would it still be good and germinate if I used this one? Or just use a totally dry one?


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Re: AGAR ENVY! (Anything and All things agar!) [Re: psycho_nauticus]
    #27671933 -



Is this myc? This is only two days worth of growth…wayyy faster than what I’m used to (though I’m a total noob).

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Prakriti]
    #27671934 -

nah


--------------------
- Sandbag Tek - How To Sterilize Spawn Bags - All About Static Pressure / Pressure Drop for DIY Flow Hoods - Sandman's LC Tek-

Marijuanaut escapes earth to cultivate - Grow-room is church temple of the new stoner breed

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Re: AGAR ENVY! (Anything and All things agar!) [Re: sandman420] * 2
    #27671958 -

sandman420 said:
nah


That’s definitely mycelium, just not the good kind


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Mehhh



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Re: AGAR ENVY! (Anything and All things agar!) [Re: nektar61] * 1
    #27671967 -

nektar61 said:
sandman420 said:
nektar how did that culture preform?


OK, Wes377 showing Z inspired me. I pulled mine out of cold storage (from 6 months ago) and put to new agar, will put to grain if it performs well.

While I was doing that I also pulled some Alacabenzi and Orissa  T3 mycelium on agar from the fridge that never went to grain, and put to plates.

Also, I found a big food prep tub, like would be used to make salad in a cafeteria, at Salvation Army. It's about 3 inches high. I'm using it upside down as a stand in front of my flowhood, to get my work up into the sweet spot. Is shown in bottom photo:






Ya this Z mycelium is amazing it's making me really enjoy agar work.
  Really like the ropey look.
  Nice set-up you have there .


--------------------
Within cells interlinked
Within cells interlinked

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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