nektar61 said:

Laurabelle784 said:

Are any of these worth using for T2? They are all fuzzy and not seeing rhizo like I have with B+ plates... Thanks!

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Several of those have a good chance of making a more-rhizo T2.

Plates 2, 4, 6, 8, and 10. haha. All even.  And maybe from around noon or 1 of what's showing in most of those.

I'd T from some of those, but also send some of same parts of those even-numbered plates to grain.

6 oclock and 9 oclock of plate 11 looks rhizo-transfering too.

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Infinite Monkeys said:

Potato dextrose honey agar seems to be working well.

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Myc Hunt said:

Infinite Monkeys said:

Potato dextrose honey agar seems to be working well.

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Another mushroom that’s gunna grow up with penis in its name
*adds to list*

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This is a PESA culture. Perhaps the PE in PESA now has a new meaning.

Infinite Monkeys said:

Myc Hunt said:

Infinite Monkeys said:

Potato dextrose honey agar seems to be working well.

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Another mushroom that’s gunna grow up with penis in its name
*adds to list*

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This is a PESA culture. Perhaps the PE in PESA now has a new meaning.

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TheDoobsker said:

Does this mean albinism is likely epigenetic and not mutations in the genome?

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Albinism is definitely due to a mutation of the genome. Mushroom phenotypes are the product of two haploid DNA sets being expressed in a variety of combinations at varying stages in development and in different physical parts of the mushroom. While albinism is generally recessive it is possible that an albino mushroom could contain one haploid set of DNA that does not have the mutation and thus produce non-albino fruits from a MS grow and possibly even non-albino fruits from a clone culture depending on the nuclear content of the tissue biopsy...or another mutation occurred somewhere in DNA replication causing regular pigmentation in the plate pins. There's an interesting article you might like somewhat related that I've posted before:

https://www.pnas.org/content/115/17/4429


Also, a pretty PE MS plate:



(Mind the aggressive wrapping)

tom613931 said:

Beginning of adventure for first flush!

I swabbed 4 plates with KSSS x APE on 2-12-22.

Pictures below are growth so far.





On 2/22/2022 I did a transfer following @Infinite Monkeys advice taking all mycelium from the largest colony on the blue plate.

Are the remaining plates worth doing anything with?  I have some jars ready to go I could inoculate from a grow kit. (I've smarted up to the grow kit mistake and will be following Bod's TEK from grain to flush)

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SandwichMan said:

What do you see here? I’m on the new side of this and am looking for advice.
Do these look clean to you?
Am I selecting from the right places when I’m taking transfers?
Any insight is much appreciated. Thanks.

1.
O t2
2. 
B+ t2
3.
B+ t2
4.
O t2
5.
O t2
6.
B+ t3
7.
B+ t3
8.
B+ t2
9.
B+ t2
10.
B+ t4

B+ t4

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multifractal said:

Albinism is definitely due to a mutation of the genome. Mushroom phenotypes are the product of two haploid DNA sets being expressed in a variety of combinations at varying stages in development and in different physical parts of the mushroom. While albinism is generally recessive it is possible that an albino mushroom could contain one haploid set of DNA that does not have the mutation and thus produce non-albino fruits from a MS grow and possibly even non-albino fruits from a clone culture depending on the nuclear content of the tissue biopsy...or another mutation occurred somewhere in DNA replication causing regular pigmentation in the plate pins. There's an interesting article you might like somewhat related that I've posted before:

https://www.pnas.org/content/115/17/4429

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Awesome, thanks for the explanation That makes sense

I'll check out the article

Wes377 said:

Z-Strain...First time getting the ropey thing.
Used less agar in the batch.

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I found Z pretty rhizo too. These are all Z:

nektar61 said:

Wes377 said:

Z-Strain...First time getting the ropey thing.
Used less agar in the batch.

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I found Z pretty rhizo too:

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