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OfflineVibetyme
Smoke 1
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Registered: 06/08/21
Posts: 1,763
Loc: Florida
Last seen: 7 hours, 9 minutes
Re: LAGM v. 2.024 [Re: Pnin] * 2
    #28605737 - 01/01/24 11:51 AM (26 days, 19 hours ago)

Vibetyme's  LAGM 2024

Albino Riptide

Starry Night APE

Toque


1/10  My plates are finally nocced up. I dipped my swabs in  sterile water to hopefully knock off some surface contams and hydrate the spores. Then I stuck the tip down in the surface of first cup in five places, on the second cup I just stabbed it in and called it good. I'm stoked!





We have lift off on all them thangs! Super stoked about these varieties! 




Edited by Vibetyme (01/22/24 01:15 AM)


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OnlineEniQma
Registered: 11/28/23
Posts: 471
Last seen: 3 minutes, 44 seconds
Re: LAGM v. 2.024 [Re: EniQma] * 3
    #28605803 - 01/01/24 12:44 PM (26 days, 19 hours ago)

Quote:

EniQma said:
Ok, so I’m going to make this the post I add to right?

I will be starting germ plates on 1/1
I’ll be doing no pours in small recycled glass jars with polyfill filters.

I have these to choose from, maybe someone could help me pick 3 since I already have a number of projects underway.

Jack Frost
Ghost
Wild Florida Cube
Natalensis
Pan Jambo
Thai Lipa Yai
Hillbilly
Pan BVI
GWM
Ps. Cyanescens
Wild ovoids I found

Seriously though, my interest is yours so lmk which 3 I should do. I wanna see some diversity around here!

I hope this isn’t going to get me disqualified!


So it’s 12/28/23 and I got my agar cooked.

I usually just wing it but this time I made a mental note to share with you fine folks. Could be soft, could be hard, time will tell…
I brought 250g water (ghetto I know) to a boil and slowly added 2.5g agar agar powder and 2.5g brown rice flour while whisking with a fork.


That was enough to make 9 of these plates(actually jars) and make them thicc. Just the way I like it 😉
Too bad I could only fit 8 in my presto

Prompting me to do a second batch.

I let the cooker vent for 10 min without the weight. Timer begins when the stopcock?(I think it’s called) was fully erect.

And once reaching 15psi I cooked for 20min

And two pro tips for any noobies,
1. Take them out hot to solidify on a flat surface.
2. Use a piece of paper towel between polyfill and foil to prevent issues with bacteria(that could occur prior to inoculation)


It’s 1/1/24 Happy New Year!
To kick it off I chose…
1. Natalensis
2. Ghost
3. Wild Ovoid

First I let my filter run 10 min. I keep it covered so nothing settles on the surface anyway, but it’s just a precautionary measure.

Then I wipe the desk and grates down with 70% iso


I proceed to do the same with my jars making sure to get up under the rim of the lid with the paper towel


Then the prints come out, and instead of using loop I just use my sterilized qtips.


It’s hard to take pictures while doing the work because I like to be fast and thorough, but I keep them as close to the flow as possible without touching and never cross anything in the path of flow from the fan to the media.




Edited by EniQma (01/01/24 03:04 PM)


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Invisiblebigfootscreepyuncl
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Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
Re: LAGM v. 2.024 [Re: EniQma]
    #28605830 - 01/01/24 01:00 PM (26 days, 18 hours ago)

Very nice setup! I'm still scraping by in a SAB but it gets the job done. I doubt I'll get around to streaking any plates today but time will tell


--------------------



I :heart: 5318008


NOT a virgin!


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InvisibleMojaveMyc
Fractal Farmer
Male

Registered: 05/08/21
Posts: 655
Loc: Colorado
Re: LAGM v. 2.024 [Re: bigfootscreepyuncl] * 1
    #28605862 - 01/01/24 01:27 PM (26 days, 18 hours ago)

First (tiny) update is in! Happy New Year mush fam! I’m looking forward to growing along with you guys :mushroom2:


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OfflineAndImTheHighOne
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Registered: 12/06/23
Posts: 44
Last seen: 12 hours, 14 minutes
Re: LAGM v. 2.024 [Re: MojaveMyc]
    #28605871 - 01/01/24 01:34 PM (26 days, 18 hours ago)

Updated! Still very much a learning experience with this, so you'll have to excuse the lack of pictures and details of my actual processes initially. I'm still using my phone to essentially read TEKs as I go, and I'm not nearly as bad ass as rawdog-countertop-guy, so most of my pics will be of finished products.


--------------------
"[Y]ou've already bludgeoned and abused the mycelium so at least do it justice and thoroughly mix it into the substrate :lol:" ~ Bigfoot

My LAGM 2.024


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InvisibleReverendMyc

Registered: 03/29/19
Posts: 1,494
Re: LAGM v. 2.024 [Re: AndImTheHighOne]
    #28606088 - 01/01/24 04:15 PM (26 days, 15 hours ago)

Welcome to the grow along. The more you share about your process and pictures that you include, the more the fine folks around here will be willing to help or learn from your methods, miracles and mistakes. That is kind of what this thread is all about.

I have updated my OP with preliminary plating of the planned prints and added a some subgenre of subtropicalis.


--------------------
LAGM 2.024
Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any more
How to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice.
Don't Panic




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Offlinemnj
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Registered: 11/23/08
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Re: LAGM v. 2.024 [Re: AndImTheHighOne] * 3
    #28606097 - 01/01/24 04:29 PM (26 days, 15 hours ago)

Hey Y'all, I'm in


Gonna give pans a good effort this year in addition to the usual cubey cubes.

I'd like to plan some small outdoor runs in pots and might tack on some novelty grows for funsies.

Gonna work with what I have on hand, but maybe more if I get the inspiration. Who's to say?

Penis Envy  abandoned for LAGM due to weak/weird growth on germ and t1 plates
Albino A+
Pan Cambo Thailand Suphanburi Goliath
Pan Cyan Hawaii

Let's boogie :awedance:


Update    Update


January 2, 2024

Got 'em streaked and labeled just before midnight.  Gotta love havin plates and syringes in the closet.

Gear: SAB, plates, syringes, inoc loop, torch, scotch, tunes



Check out that time stamp on the stove :rockon:




Update    Update


January 7, 2024

Hey y'all

I've been enjoying the spirit of LAGM and seeing everyone's updates.

When I started a week ago I overlooked the fact that one of the inspirations for LAGM was to demystify the process for newcomers, so from here on out I will include details and attach links when I can.  I've never written down what I do so I guess this will also serve as my personal reference for whenever I come back from taking breaks.  Everything I do has been gleaned from other members and so many awesome threads



My Agar Recipe:

The plates I used were pastywhyte's pasty plates using Pastywhyte's Easy Agar Tek.  Here's the recipe I had in my phone notes from 6 years ago.  I don't know how true this recipe is to the original but it works for me and I do it.  I think it makes about 6-8 pasty plates depending on the container I use (idk, it's been months since I've made it):

  • 1.5 - 1.75 grams agar powder (I stay on the lower end for softer agar, better for germination i think?)
  • 1.5 - 2 teaspoons instant potato flakes (no flavoring you freaks) (I stay on the lower end or even cut the amount in half if I want bolder/more-vigorous growth because I think I read that's a thing?)
  • 1/2 cup water
  • Drop of honey
  • ~3 drops of food coloring (I like blue) (apparently red food coloring is anti-fungal?! Might be an urban legend but the last time I used red things didn't go so well...coincidence?...readjusts tin foil hat) 

Recipe is easily doubled, just use twice as much of each ingredient.


My containers:

Lately, because I think Glad stopped making the 4 oz round containers (and the ones I ordered off amazon must have sat in a hot warehouse for years because none of the lids fit), I've been using wide mouth half-pint jars with a plastic (no rust!) Ball brand black/grey plastic lid with a 1/8" - 1/4" hole drilled in it (or whatever size my Dremel bit is; a hot nail will do just fine), covered with micropore tape (I'm sure polyfil or other alternative will work).  The high and straight walls of the jar are not ideal but I've been managing just fine in my still-air-box, just have find a good angle so I minimize the amount that the unsterile handle of my scalpel/inoculation loop hovers over the surface).


How I prep the agar for plates:

I add the water to an egg sauté pan (I like the low/angled walls for whisking) and add potato flakes, let 'em cook a few minutes to incorporate (keep it at a simmer so the water level doesn't evaporate too much, i try to keep the water to agar ratio pretty stable) then add drop of honey and then agar powder a little at a time, stirring with a whisk so it doesn't clump, finally i finish with the food coloring.  I spoon it into my containers a tablespoon at a time (the larger spoon in your spoon drawer) and then I add the remaining mixture by the spoonful or half-spoonful, depending on what's left in the pan, jar-by-jar until the depth looks good (some containers have a rise in the bottom-middle that I try to overshoot). 

Finish by putting on container lids, fold up half-a-paper-towel into a square and put it on the lid, then wrap the whole thing in foil.  I fold and roll the excess foil to the sides of the container, keeping the bottom as flat as possible, because that means the agar disc will be as level as possible when it cools after the pc cycle.


Prepping the PC

I prep the pc by loosely crumpling up foil and lining the bottom of the pc so that my trivet will sit several inches off the bottom, then add 1.5" - 2" of water, and put the trivet on top of the foil.  This helps keep the plates away from the boiling water bubbles.  Load the plates and put lid on pc.

I vent the pc for 10 mins then I put on the weight.  Once the pressure rises to 15 psi, I pc for 45 mins, then turn the burner off and allow the pc to return to 0 psi naturally.


How I store my containers:

This I learned from Josex in his Josex' Guide to No-pour Agar tek: After pc reaches 0 psi, I take the plates out of the pc while still hot and let them settle on a flat surface so the agar disk (hopefully) solidifies with a flat surface (in a short long rectangular plastic tote with a lid where they live in my closet until I need to use them.  I put the lid on the tote after they cool so condensation doesn't build up inside the tote.  A few paper towels laid in the tote will soak up ambient moisture.)

The plates I used to swipe spores on January 1 had been stored for several months.  The benefit of this is they had no condensation on the sides!  My visibility with no-pour plates was greatly improved!!!  Edit: these plates had better visibility but they were also dry as hell and not great for germination.


How I Inoculate:

For the inoculation process I used a collection of inoc loop info that I gathered and internalized from reading/searching on here, but  BOD's DIY Inoculation Loop TEK is a great place to start.  One tip I picked up from other members is to cool your hot and freshly-flamed loop in the agar of your receiving dish before shooting/swiping spores onto the loop.  A hot loop can cook and minimize the germination ability of your spores.  I was in a hurry and jammin tunes and drinkin scotch when I swiped plates on the 1st so I forgot this step, but I made two plates of each variety and I only sterilized my loop between varieties, so at least the second plate received a streak with a cooled-down loop :thumbsup: Edit: For the second round of plates I just dropped the spore solution directly from the syringe onto the agar


A few days ago I believed I saw germination (is that a white clump starting to form or is that just the light reflecting off a small indentation left by the inoc loop?!?)

Today I for-sure have germination on two plates.  The small white dots in the photos are dried agar/potato flake residue on the walls of the container from where the mix boiled up during the pc process.  The denser, bigger, whiter dots are spore germination :smile:


AA+




Pan Cyan Hawaii



:cheers:


Update    Update


January 11, 2024

Seeing some germination on a Penis Envy plate



Half my plates have growth but all growth is happening very slowly.  I think it's a combination of cold temps (low-mid 60s) and using old plates that are kinda dry. 

I made fresh plates a few days ago so I will noc some up this weekend (my pan cambo has not germinated and I wanna give that another go, plus I'd like to get more diversity with AA+) and I may do some transfers if the myc has moved a significant distance from the germination points (it's all going very slowly)


Update    Update


January 14, 2024

Woke up nice and early so I cleaned off the kitchen counter and brought out the SAB. 

Shot some of the pasty plates I made last weekend with mss of Pan Cambo Thai and AA+, two plates of each. 

Made T1 transfers of Penis Envy and AA+.  I wasn't impressed with either variety's germ plate, but thought I'd make the transfers and see what happens.

No pics of these plates because the condensation is ridiculous.

My Pan Cyan Hawaii germ plate is coming along with several germination points.  One has spread far enough for transfers but the other two are not yet ready so I'm gonna let it ride a little longer:



Gonna cook more plates today so I'll be ready for transfers whenever the donor plates are ready


Update    Update


January 18, 2024

My original AA+ plate had only germinated in one spot as a bright white fuzzy mass that reached as high as it did wide. Took transfers to a few plates anyway to see what was up (because the Oak Ridge I did last year also started out growing very vertical on the germ plates but performed super well!). Anyway, the two T1 plates are growing fast and fuzzy and I don't trust em. Today I put em in a ziploc and then a Tupperware and set em aside to see what happens. Either I've got two dope mold plates or I'll have plins in mid February. My gut says mold.

I've already noc'd up a few fresh AA+ plates bc I've never grown this variety and wanna give it a go.  Germination is happening on both so looking forward to making transfers soon.

I ditched my PE because it was a translucent top crawler and overall the agar it touched looked gross. Idk wtf it was but it wasn't worth my time.  It looked like myc in its growth patterns but was only visible from the way it reflected under a flashlight.  I already have a few PE clone cultures going from 2023 so I will use my time and space to focus on those. Might do some top fruited cakes if I want more options but that won't be part of this thread.


Update    Update


January 24, 2024

Did some T1's today of Pan Cambo, Pan Cyan and AA+.  Took transfers to multiple plates.  Some of the transfers came off the xacto knife clean and others I had to wiggle and waggle off the blade :shrug:

Here's a pic of some of them .  Germ plates on bottom, T1's on top.



You can see some non-LAGM cakes in the background that I shot with mss of Pan Cambo and Pan Cyan during the same SAB session.  I always feel like I'm missing out on a good culture when I make agar selections so I plan to top-fruit these cakes to look for clone candidates. 

Anyway, I know this isn't technically LAGM, but thanks to the LAGM process I know these two spore syringes are what I would call "clean" because they have not shown signs of contamination on agar, so I thought I'd go for it. :thumbup:

Cakes were made of store-bought black kow manure + oats + verm and pc'd 90 mins.

I was inspired by the following wonderful threads: V.L's EF-tek (Exotics Fanaticus cakes), MacMerdin's MacMerdin's Simple Pan-Cakes, MudaFuka's Updated Bottle Tek New and improved, and Bod's PF tek for cloning on the EZ.


Edited by mnj (01/24/24 08:47 AM)


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OfflineSeventhMushroom
just a tiny agar pin


Registered: 12/30/22
Posts: 55
Last seen: 1 day, 22 hours
Re: LAGM v. 2.024 [Re: mnj] * 2
    #28606139 - 01/01/24 05:07 PM (26 days, 14 hours ago)

Updated post. Got to use my new flowhood, got to use a spore print for the first time, got some gourmet cultures started in jars while I was at it. Let's hope for some mushrooms :laugh:



--------------------
LAGM 2024


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OfflineGogoBonkers
Just another pilgrim
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Registered: 07/09/23
Posts: 87
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Re: LAGM v. 2.024 [Re: SeventhMushroom]
    #28606268 - 01/01/24 06:29 PM (26 days, 13 hours ago)

Updated Op

Happy new year 2024!


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OfflineNoncense
Saul Pamets
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Registered: 12/23/21
Posts: 80
Loc: PNW
Last seen: 3 days, 5 hours
Re: LAGM v. 2.024 [Re: GogoBonkers]
    #28606287 - 01/01/24 06:40 PM (26 days, 13 hours ago)

Main post updated, sorry it took me a few days just wrapping up my year and have been under the weather and busy myself. As a reminder, the rules are in the main body of the post, they are very literal. Spores to agar, after that go hog wild with your grow method.

Thanks everyone for participating, ill be claiming my own spot shortly.


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OfflineNoncense
Saul Pamets
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Registered: 12/23/21
Posts: 80
Loc: PNW
Last seen: 3 days, 5 hours
Re: LAGM v. 2.024 [Re: Noncense] * 2
    #28606311 - 01/01/24 06:53 PM (26 days, 12 hours ago)

Noncense LAGM 2024


MENU
Cubensis Vars.
TD
Steel Mag 5
Albino Thrape #1 (Nug) F1
A. Thrape #2 (FC) F1
A. Thrape #3 (LB) F1
MORE TBD
Exotics:
Ps. Semperviva

1/1/24
Spores swabs to agar standard 10 grams lme 10 grams agar recipe, green and blue color for funsies.


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OfflineWomble
Wombling Free

Registered: 09/12/23
Posts: 97
Last seen: 2 days, 11 minutes
Re: LAGM v. 2.024 [Re: Noncense] * 1
    #28606602 - 01/02/24 02:59 AM (26 days, 4 hours ago)

I'm in, as luck would have it I recently pored my first agar plates ever and streaked a bunch of them on new years day. So this has been timed perfectly to participate in this.

I'll be trying to grow:
MVP
Redboy
GT

30/12/2023
Poured my first plates I have ever poured today. Recipe was 500ml water, 10g of Telephone brand Agar Agar powder and 10g of light malt extract powder. Followed guides on here and vented PC for 10 mins and PC'd for 20 mins. Allowed PC to depressurize after the cycle and allowed agar to cool to around 48c and poured my plates - got 19 in total.



01/01/2024
Smeared 10 plates in zig zag pattern with 5 varieties using my DIY inoculation loop (only 3 varieties as part of this LAGM). 2 plates per variety for some sort of redundancy.

Both MVP and Redboy spores were donated to me from a generous member in the marketplace and the GT spores came from my personal prints. The other 4 plates also came from my personal prints but I cannot be sure exactly what they are so i'm not including them as part of this.



03/01/2024
No noticeable growth on any plates - hoping that my agar mix was not too firm for a germination plate - fingers crossed they are successful as the MVP and Redboy prints I received were very light and I may only have one more attempt using them.

05/01/2024
I have visible germination on 5 out of 6 plates so far. Stoked about the MVP and Redboy as they are both new genetics I received. Will try and play around with lighting to get some decent pictures over the next few days.

Germination:
GT - 1 of 2
MVP - 2 of 2
Redboy - 2 of 2

08/01/2024
Germination pics, apologies for the picture quality but plates are hard to take photo's of any tips from anyone?:

MVP - both plates look like this:


Redboy - both plates look like this:


GT - the 1 plate where I thought I saw germination had heavy condensation and so I put a hot cup of water on the plate, it looks like a lot of condensation got washed around the plate and looks heavily bacterial?

I think I will have to pour some new plates and start these again from spores or chalk these up as a failure and just run with the other 2 strains.




Edited by Womble (01/08/24 03:56 AM)


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OfflineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
Last seen: 9 hours, 25 minutes
Trusted Cultivator
Re: LAGM v. 2.024 [Re: Noncense] * 6
    #28606727 - 01/02/24 07:45 AM (26 days, 2 minutes ago)

I was planning to just lurk in the shadows but non still hasn't update the prizes so I'll just tell you funguys and fungirls.

I've put up 5 Ps tampanesis (F1 off a wild South Carolina find), 3 Ps subtropicalis/semperviva and 3 cubensis var ESS prints.


--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


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OfflineMonkeyJesusFresco
am i suspended in agar?
Male


Registered: 10/09/12
Posts: 3,306
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Last seen: 9 hours, 55 minutes
Re: LAGM v. 2.024 [Re: SirPsycho] * 2
    #28607867 - 01/02/24 11:45 PM (25 days, 8 hours ago)

ah what the hell, I'm in :shrug:

tba

guess I'll give it a go :shrug:


--------------------
LAGM v 2.024
- endo cabendo


Edited by MonkeyJesusFresco (01/05/24 01:38 AM)


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OfflineSirPsycho
Purple Belt in Google-Fu
I'm a teapot User Gallery


Registered: 01/01/20
Posts: 6,900
Loc: Rent free in your head
Last seen: 9 hours, 25 minutes
Trusted Cultivator
Re: LAGM v. 2.024 [Re: MonkeyJesusFresco] * 7
    #28608236 - 01/03/24 09:56 AM (24 days, 21 hours ago)

Ok, I guess I'll grow some mushrooms. This is a wild print sent to me by a buddy in Florida. Found in 2022



--------------------
:pm:Ask me about free Ps tampanesis, Ps subtropicalis and Ps cubensis (ESS) prints:pm:
Balance in life is like running on ice.

  🅑🅞🅣🅣🅛🅔 🅖🅐🅝🅖

"Mist your balls and fan your asshole" - Pandaskis, 2023


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Invisiblebigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
Re: LAGM v. 2.024 [Re: SirPsycho]
    #28608958 - 01/03/24 09:28 PM (24 days, 10 hours ago)

Very minor update with no pictures. Got Pan. cyan BVI, Bispo, NecD, Wild Coast and Jambo all streaked..and now we wait!


--------------------



I :heart: 5318008


NOT a virgin!


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OnlineEniQma
Registered: 11/28/23
Posts: 471
Last seen: 3 minutes, 44 seconds
Re: LAGM v. 2.024 [Re: bigfootscreepyuncl]
    #28608960 - 01/03/24 09:30 PM (24 days, 10 hours ago)

:creepylurker:


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OfflineTri-Polar
Paramecium Brain
I'm a teapot User Gallery


Registered: 08/23/18
Posts: 374
Loc: purgatory
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Re: LAGM v. 2.024 [Re: EniQma] * 2
    #28608967 - 01/03/24 09:36 PM (24 days, 10 hours ago)

Dammit im in, maybe this will finally be the year I actually stay consistent with documenting my LAGM

I will be growing some BAPER-R

January 7th
Put spores to agar, first time using an inoculation loop so I feel like I fucked it up, also was too busy to pour new plates so I used an old PDA plate from a batch I was pretty displeased with but I couldnt care less and probably neither could the spores so here we go babyyy


January 10th
Put more spores to agar this time with a swab, really hit me in that moment- you can make metric fuck tons of swabs from a single print if you're so inclined, neat.



January 17th

good news we got germination, bad news im sick af with covid

Win some lose some


Edited by Tri-Polar (01/17/24 01:48 PM)


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OfflineMonkeyJesusFresco
am i suspended in agar?
Male


Registered: 10/09/12
Posts: 3,306
Loc: South East USA Flag
Last seen: 9 hours, 55 minutes
Re: LAGM v. 2.024 [Re: Tri-Polar]
    #28609022 - 01/03/24 11:38 PM (24 days, 8 hours ago)

what's BAPER-R?


--------------------
LAGM v 2.024
- endo cabendo


Edited by MonkeyJesusFresco (01/03/24 11:38 PM)


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OnlineEniQma
Registered: 11/28/23
Posts: 471
Last seen: 3 minutes, 44 seconds
Re: LAGM v. 2.024 [Re: MonkeyJesusFresco] * 1
    #28609123 - 01/04/24 05:32 AM (24 days, 2 hours ago)

Black ape revert redspore….
Duh 🤦‍♂️


Edited by EniQma (01/04/24 06:25 AM)


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* First post : A new passion, thank you ! & Jalisco, Tampanensis, Hoogshagenii aka Semperviva & Co
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Aendir 2,299 27 08/08/19 03:09 PM
by Tweeq

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