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APE Cultivation
    #27488264 -

So I recently got a APE spore syringe however the inside looks more like a liquid culture. I've put some on agar a few days ago and I'm still not seeing any mycelium how long does this strain typically take to grow?

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Re: APE Cultivation [Re: TurtleLove]
    #27488269 -

APE is 1 of the slowest to germinate. Give it a couple of weeks or so and see how it looks.


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Re: APE Cultivation [Re: Blazer420]
    #27488388 -

Albinos have clear spores which is prob why it looks that way.


Spores can take up to a couple weeks to germinate but ime if they haven’t started in a week they’re probably not going to.


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LAGM2020

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Re: APE Cultivation [Re: A.k.a]
    #27488465 -



I've had the same experience, but once I looked under the scope I found that they were indeed spores.



Usually takes about a week to germinate on agar, maybe longer if pf tek. It's generally much slower growing than other cubes but some experienced cultivators have found cultures that grow pretty quick.


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Re: APE Cultivation [Re: Lemgrub] * 1
    #27488602 -

Nice microscope work! Mine came just like that too, bur I've been fighting sloppy work n such, or else I'd have some fruits by now.

Keep Tuckin' :rockon:


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Never give up, never surrender.

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Re: APE Cultivation [Re: Failboat]
    #27488615 -

I also had the same experience

Nice scope work, good to know it’s spores not culture :super:


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Re: APE Cultivation [Re: SawCeeJack]
    #27488636 -

Like everyone else, I had trouble with a vendor syringe like that. 5-6 plates with a single drop had no germination.

I overcame this with rage- instead of a drop, I squirted several mls into 2 plates so it was pooling very slightly. Just enough that there's a slight film of liquid across the plate.

If it germs, the myc will initially look like a wet pimple. Tilt the plate away from the pimple so excess moisture is removed, it will dry and fluff up in a day or two and then transfer quick.

Rage tek increases the chance of contam, but it also increases the chance of germ if you've got a low spore count syringe. I've done it several times with success on mss I couldn't get to germ normally.


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Re: APE Cultivation [Re: bagga]
    #27488693 -

Every time I squirt that much onto a plate, I get bacteria around the edges


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Re: APE Cultivation [Re: SawCeeJack]
    #27488772 -

With a hard start syringe I don't really care much if it goes bacterial as long as I get some viable myc to transfer.

You could also try dropping several mls onto a sterile swab, and dropping the bud into a plate. That will concentrate the spores and improve chances. Swabs usually create tall aerial myc which is great for escaping bacteria.

My goal with a stubborn mss isn't a clean plate, just a viable transfer. Germ plates are presumed dirty anyway.


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For personal religious use only. Embrace infinity for a healthier mind.

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Re: APE Cultivation [Re: bagga]
    #27488984 -

Good idea about the swab, thanks :takingnotes:


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Re: APE Cultivation [Re: SawCeeJack]
    #27490194 -

I just recently had success by going straight to grain. Same looking syringe. Took several days longer than normal but all of them worked.

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