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multifractal
Student

Registered: 05/08/15
Posts: 1,019
Loc: Cumtown

Re: AGAR ENVY! (Anything and All things agar!)

1
#27474989 - 09/19/21 09:59 PM (4 years, 11 months ago)

Lostkeys said:

Blob pins?

Seems so. Although I've cloned blobs on plates before and subsequently grew out normal looking mushrooms. Plates arent the best environment in which to predict the phenotype of mushrooms when grown under more optimal conditions.

Psilocybe subtropicalis plate pin. This plate was inoculated on 7/26. Glad to see my culture produces fruit as I have 3 tubs currently colonizing.

Edited by multifractal (09/19/21 10:49 PM)

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aNewBeginning

Registered: 06/26/21
Posts: 708

Re: AGAR ENVY! (Anything and All things agar!)

[Re: A.k.a]
#27475056 - 09/19/21 11:51 PM (4 years, 11 months ago)

A.k.a said:

Is this what you guys are using??

Extras:

Lostkeys
Fractal Thought Pattern

Registered: 06/06/09
Posts: 1,355
Loc: Sirius
Last seen: 2 years, 9 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: aNewBeginning]
#27475190 - 09/20/21 04:44 AM (4 years, 11 months ago)

:whathesaid:

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So heavy I fell through the earth...

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Guerrilla
Original Asshole

Registered: 01/30/21
Posts: 3,208
Loc: United Kingdom

Re: AGAR ENVY! (Anything and All things agar!)

5
#27475221 - 09/20/21 06:10 AM (4 years, 11 months ago)

Tidal wave 4 T2

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Being pissed on does not make you a real man.

...OR DOES IT?

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rockyfungus
dirty

Registered: 03/01/21
Posts: 1,097
Loc: Front range
Last seen: 1 month, 30 days

Re: AGAR ENVY! (Anything and All things agar!)

[Re: aNewBeginning]
#27475348 - 09/20/21 08:33 AM (4 years, 11 months ago)

aNewBeginning said:

A.k.a said:

Is this what you guys are using??

Sprouts had a huge bottle of the Now brand. Doubt I'll ever get through it.

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Big_Dub
I'm just some guy

Registered: 01/12/11
Posts: 3,642
Loc: Los Angeles

Last seen: 19 hours, 44 minutes

Re: AGAR ENVY! (Anything and All things agar!)

[Re: rockyfungus]
#27475605 - 09/20/21 11:59 AM (4 years, 11 months ago)

rockyfungus said:

aNewBeginning said:

A.k.a said:

Is this what you guys are using??

Sprouts had a huge bottle of the Now brand. Doubt I'll ever get through it.

If you have a winco foods (in-between a Kroger and a costco), they sell nooch in a barrel. Just scoop as much as you want.

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Atomsplit

Registered: 01/16/21
Posts: 1,578
Loc: SAB

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Big_Dub]
#27475707 - 09/20/21 01:30 PM (4 years, 11 months ago)

I'm a fan of yeast.

A few months ago I thought that it made my cultures grow too thick but I was really inexperienced & still am lol.

Thanks for everyone helping along. Props to d3 and his great agar thread. Lowering my lme a tiny bit seems to be helping too.

I have this clone T-6 that I cant seem to clean up. Would anyone have any tips? Thanks in advance!

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.

Edited by Atomsplit (09/20/21 02:04 PM)

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Ora
Stranger

Registered: 07/09/21
Posts: 734
Loc: in your heart
Last seen: 4 years, 8 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Atomsplit]

1
#27475809 - 09/20/21 02:23 PM (4 years, 11 months ago)

maybe it is clean? sometimes i get that similar thing at your 9oclock area. i wonder if it has to do with the way the transfers are taken like the edge side vs the inner side when you take a transfer and place it on the new plate.

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Lostkeys
Fractal Thought Pattern

Registered: 06/06/09
Posts: 1,355
Loc: Sirius
Last seen: 2 years, 9 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Ora]

2
#27475875 - 09/20/21 03:14 PM (4 years, 11 months ago)

Yeah, I don't think that's dirty at all. I see clear sectoring and I'm pretty sure the questionable spot is just a sector of tomentose mycelium. I would still take another transfer though, just so you get uniform growth.

For instance, this ain't dirty, and there's a big sector of tomentose growth at the bottom...

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So heavy I fell through the earth...

Edited by Lostkeys (09/20/21 03:18 PM)

Extras:

Atomsplit

Registered: 01/16/21
Posts: 1,578
Loc: SAB

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Lostkeys]
#27475934 - 09/20/21 03:56 PM (4 years, 11 months ago)

Thanks ora and lost key you both brought up good points.

That melmac plate is a beauty. I'd put that to grain!

For instance I'm more confident in this plate but still took transfers off of it just in case.

Taking too many transfers can be a rabbit hole lol. Something I'm keep reminding myself not to do [thanks AKA!] but I think everything is suspect lol.

I've tossed so many bacterial jars of grain it's embarrassing.

Thanks again!

--------------------

.

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Guerrilla
Original Asshole

Registered: 01/30/21
Posts: 3,208
Loc: United Kingdom

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Lostkeys]
#27475979 - 09/20/21 04:25 PM (4 years, 11 months ago)

Lostkeys said:

Yeah, I don't think that's dirty at all. I see clear sectoring and I'm pretty sure the questionable spot is just a sector of tomentose mycelium. I would still take another transfer though, just so you get uniform growth.

For instance, this ain't dirty, and there's a big sector of tomentose growth at the bottom...

I mean... he’s on T6.. I don’t think his issue would be solved with taking another transfer. He already said he can’t “clean it up.” Never know though...

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Lostkeys
Fractal Thought Pattern

Registered: 06/06/09
Posts: 1,355
Loc: Sirius
Last seen: 2 years, 9 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Atomsplit]
#27476071 - 09/20/21 06:07 PM (4 years, 11 months ago)

Atomsplit said:

Thanks ora and lost key you both brought up good points.

That melmac plate is a beauty. I'd put that to grain!

For instance I'm more confident in this plate but still took transfers off of it just in case.

Taking too many transfers can be a rabbit hole lol. Something I'm keep reminding myself not to do [thanks AKA!] but I think everything is suspect lol.

I've tossed so many bacterial jars of grain it's embarrassing.

Thanks again!

Yeah man, I took several transfers from those thick ropes. Waiting for the T3 to grow out then it's on to test runs.

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So heavy I fell through the earth...

Extras:

multifractal
Student

Registered: 05/08/15
Posts: 1,019
Loc: Cumtown

Re: AGAR ENVY! (Anything and All things agar!)

3
#27476242 - 09/20/21 08:47 PM (4 years, 11 months ago)

Wow another subtropicalis plate pin. It seems this species really favors the edges of the plate for fruiting compared with cubensis.

I had no hopes for this particular plate as I completely botched the transfer. Dropped the wedge on top of the plate lid, messily got it back on the scalpel with the help of a second scalpel, dropped it again but this time on the plate in an awkward spot, then accidentally touched the agar with my finger moving the wedge to the center. All of this while the music from Curb Your Enthusiasm played in my head. Kept it around just to see how contaminated it would be.

Edited by multifractal (09/20/21 08:57 PM)

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Lostkeys
Fractal Thought Pattern

Registered: 06/06/09
Posts: 1,355
Loc: Sirius
Last seen: 2 years, 9 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: multifractal]
#27476972 - 09/21/21 01:22 PM (4 years, 11 months ago)

Keep hearing about this stuff... Now to figure out how much to put in. I'm thinking 1g per 500ml like yeast?

Anybody around here used it in their agar?

--------------------
So heavy I fell through the earth...

Extras:

Guerrilla
Original Asshole

Registered: 01/30/21
Posts: 3,208
Loc: United Kingdom

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Lostkeys]
#27477011 - 09/21/21 01:57 PM (4 years, 11 months ago)

Seen it used but don’t know a lot about it. Sorghum syrup is another good one apparently. I may have to give both a try

--------------------
Being pissed on does not make you a real man.

...OR DOES IT?

Extras:

multifractal
Student

Registered: 05/08/15
Posts: 1,019
Loc: Cumtown

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Lostkeys]
#27477012 - 09/21/21 01:58 PM (4 years, 11 months ago)

I've used it before. 1 g / L is what I found to be usual.

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Hindsight
Mad Scientist

Registered: 01/24/21
Posts: 2,706
Last seen: 3 years, 4 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Lostkeys]
#27477032 - 09/21/21 02:12 PM (4 years, 11 months ago)

Lostkeys said:

Keep hearing about this stuff... Now to figure out how much to put in. I'm thinking 1g per 500ml like yeast?

Anybody around here used it in their agar?

I use the same brand but use soya peptone instead of bacteriological peptone. The amount varies depending on what I'm doing but 1g per 1000ml batch of agar as a supplement to LME is what I use for cubes. For pans I use 5g of it as a supplement to LME. I notice more consistent and rapid growth on my plates when using peptone.

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kanemush
Grumpy

Registered: 12/07/17
Posts: 1,564
Last seen: 1 year, 8 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Hindsight]

1
#27477563 - 09/21/21 09:47 PM (4 years, 11 months ago)

project i'm currently working on this is 1st transfer from spore to ms, then to agar taken from a clone i was running a few years ago.

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Lostkeys
Fractal Thought Pattern

Registered: 06/06/09
Posts: 1,355
Loc: Sirius
Last seen: 2 years, 9 months

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Hindsight]
#27477571 - 09/21/21 09:51 PM (4 years, 11 months ago)

Hindsight said:

Lostkeys said:

Keep hearing about this stuff... Now to figure out how much to put in. I'm thinking 1g per 500ml like yeast?

Anybody around here used it in their agar?

I use the same brand but use soya peptone instead of bacteriological peptone. The amount varies depending on what I'm doing but 1g per 1000ml batch of agar as a supplement to LME is what I use for cubes. For pans I use 5g of it as a supplement to LME. I notice more consistent and rapid growth on my plates when using peptone.

I figure I'll mix up like 5 test plates and compare it to my true love in additives, nutritional yeast. It would be nice to get the same growth without the sediment.

--------------------
So heavy I fell through the earth...

Extras:

Guerrilla
Original Asshole

Registered: 01/30/21
Posts: 3,208
Loc: United Kingdom

Re: AGAR ENVY! (Anything and All things agar!)

[Re: Lostkeys]
#27477724 - 09/22/21 03:31 AM (4 years, 11 months ago)

Keep us updated brotha!

--------------------
Being pissed on does not make you a real man.

...OR DOES IT?

Extras:

Pages: < First | < Back | 541 | 542 | 543 | 544 | 545 | 546 | 547 | 548 | 549 | 550 | 551 | 552 | 553 | 554 | 555 | 556 | 557 | 558 | 559 | 560 | 561 | Next > | Last >

Shop:

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Spores for European Microscopy

Mono Tub Substrate

Isolated Cubensis Liquid Culture For Sale

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