Home | Community | Message Board
You are not signed in.
Sign In New Account
Forum Index Search Posts Trusted Vendors Highlights Galleries FAQ User List Chat Store Random Growery

Kratom Eye
This site includes paid links. Please support our sponsors.

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   Myyco.com Golden Teacher Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy

Jump to first unread post Pages: < First | | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 |
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Re: Making Liquid Culture [Re: mushboy]
    #27370044 -

For my growlog "Dem dicks" I did several small LC's, ~60ml each.
Did them because I thought they were going to be ready way faster, but for some reason that was not the case at all.
Seen 150-200ml LC's be ready in less time.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
Has u got Magic?

PHENO HUNTERS

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Josex]
    #27370056 -

I think they know how much space and food they have so they're not in a hurry to exhaust themselves.


--------------------
Question everything, it's very responsive.
culture storage

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Nichrome] * 1
    #27370073 -

You could actually be saying truth, sir. Something like that crossed my mind too and the colony doesn't colonize the liquid the same way it does solids.


--------------------
LC's are easy
Go big with No-pours
Clean yo shit
Has u got Magic?

PHENO HUNTERS

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Josex]
    #27370149 -

How do you guys store colonized LC for like 1-2 months in unmodified media/agar bottles?


--------------------

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: nix21] * 1
    #27370208 -

Just sit it on a shelf out of direct light.


I once got some gourmet lc mailed to me in I think .5 ml vials. :lol:


--------------------
LAGM2020

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: A.k.a]
    #27370223 -

Green cap on the vial? Poplar oyster? :laugh2:


--------------------
Question everything, it's very responsive.
culture storage

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: A.k.a]
    #27370264 -

I was wondering wont they run out of O2 if they sit for 2 months? Do LC that came out of fridge after 2 months show same colonization speed and recovery on grain as LC kept in room temp?


--------------------

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: nix21] * 1
    #27370277 -

These are T1 from plates started with LC that sat in a 5 ml sealed vial in the fridge for 2 years. Still gold. IMO it got better.



Seems like O2 needs are driven by cellular division/metabolism. I give a lot of credit to these critters for their conscious ability to change habits to conserve or maximize resources. These are creatures that have the potential to live indefinitely given the right changing circumstances. They are very smart.:super:


--------------------
Question everything, it's very responsive.
culture storage

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Nichrome]
    #27370355 -

How long can LC sit fully colonized at room temp without degradation in quality? Fridge would be ideal but I just don't have the space for it.

bodhisatta said:
If ive learned one thing growing thousands of gallons of yeast for beer fermentation when i worked as a lab tech, and seeing LCs made in other industry. Use them at peak and don't let them sit around.

All organisms start to go downhill once they've "fully colonized" the LC. Intracellular glycogen content plummets in fungal species. Translating to increased lag phases when subsequent use occurs.


This is what Bod has to say but seems like people do alright leaving LC sitting around, unless I'm misremembering and they refrigerated it or other.


--------------------
:chems:

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Lenz] * 1
    #27370531 -

Right now I’ve got a bunch of 3-5 month old lcs with filters on them that have been stored in a closet but the rooms been hitting 100f for at least a month.

I went to use some and only one worked half decent, the rest took around 10 days to show any growth but they did eventually grow.


--------------------
LAGM2020

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Nichrome]
    #27370633 -

Nichrome said:
I think they know how much space and food they have so they're not in a hurry to exhaust themselves.


:mindblown: It all makes sense now


--------------------

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: LeafyClark]
    #27370639 -

Im itchin to try another LC. Got plenty of bottles. Maybe Nats and APEU. 250ml of each. Gonna be better this time :stoned:


--------------------
Never give up, never surrender.

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Failboat]
    #27370641 -

:awedance:

Got a few broths going here too


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: mushboy]
    #27370653 -

I need to pick the best bottles I have for the job. I know I've failed from operator errors working with bottles in the past so if I can eliminate those hangups then I should be good to go. Still haven't invested in a stirrer but I like shaking em well enough, but hence the bottle selection.


--------------------
Never give up, never surrender.

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Failboat]
    #27370670 -

I use fancy salad dressing jars 330ml they are perfect



Or media botles.


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: mushboy]
    #27370676 -

I have one legit Pyrex 500ml bottle and 8 blue caps from 2 different sources. One blue ring is def fucked.


--------------------
Never give up, never surrender.

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Failboat] * 1
    #27370683 -

Go buy marzetti brand salad dressing the ranch is delicious and reuse the bottle the lids are pp.


--------------------
:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: mushboy]
    #27370685 -

mushboy said:
Go buy marzetti brand salad dressing the ranch is delicious


this is the truest thing anyone has ever said

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: adhoc]
    #27370693 -

:notsureif:
Marzetti representative? Like the chewleys gum guy right? :lol:


--------------------
Never give up, never surrender.

Extras: Filter Print Post Top
Re: Making Liquid Culture [Re: Failboat] * 1
    #27370696 -

Quirkmeister92 said:
:notsureif:
Marzetti representative? Like the chewleys gum guy right? :lol:


man I wish I'd take payment in ranch dressing haha

Extras: Filter Print Post Top
Jump to top Pages: < First | | 35 | 36 | 37 | 38 | 39 | 40 | 41 | 42 | 43 | 44 | 45 | 46 | 47 | 48 | 49 | 50 | 51 | 52 | 53 | 54 | 55 |

Shop: MagicBag.co All-In-One Bags That Don't Suck   North Spore Injection Grain Bag   Myyco.com Golden Teacher Liquid Culture For Sale   Original Sensible Seeds Bulk Cannabis Seeds   Sporeworks.EU Spores for European Microscopy

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
Threaded Mode Threaded  Previous topic Previous  View all topics Index  Next topic Next 

Similar ThreadsPosterViewsRepliesLast post
* 1st Liquid Culture, arrrgh! Seril 15,582 10 02/28/06 08:35 PM
by Limelight_Liver
* Starting a simple liquid culture method Garret_Hill 9,968 3 03/11/02 09:49 AM
by YourHighness
* Easy Liquid Culture Container Tek SixTango 21,760 7 01/17/12 04:59 PM
by dubyastep
* Liquid Cultures to Bulk Substrate Prodijal_Son 19,181 7 05/05/20 03:40 AM
by poisoned
* liquid culture failure gray1 6,481 3 07/19/01 09:37 PM
by Azure
* liquid culture using h2o2 baja 9,950 14 03/09/17 10:00 PM
by 3B3B4E
* Liquid culture inoculation of straw... possible? The FUnGal FiEnD 7,981 9 05/26/13 04:57 PM
by cronicr
* Liquid culture for alkaloids?? Dr. Slavic 973 3 01/26/03 06:29 PM
by Anonymous

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, the_chosen_one, LogicaL Chaos, 13shrooms, hamloaf, cronicr, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
95,046 topic views. 12 members, 164 guests and 90 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2026 Mind Media. Some rights reserved.

Generated in 0.028 seconds spending 0.008 seconds on 16 queries.