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InvisiblemushboyMDiscord
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Re: Making Liquid Culture [Re: pablokabute]
    #27573340 - 12/08/21 08:29 AM (2 years, 1 month ago)

:ytho:


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Invisiblepablokabute
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Re: Making Liquid Culture [Re: mushboy]
    #27573351 - 12/08/21 08:40 AM (2 years, 1 month ago)

Cuz, after all these years, I still suck at lab works.

Or our house is chocful of shit in the air,

..and it's just not possible at the moment. :smile:


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OfflineA.k.aM
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Re: Making Liquid Culture [Re: Mtbromo]
    #27573448 - 12/08/21 10:06 AM (2 years, 1 month ago)

Quote:

Mtbromo said:





Do these two jars look contam or ok? 1st attempt at it all
Thx





Like they said it’s pretty tough to know for sure, but those look good to me as far as visual inspection. If the water was cloudy at all then you’d have a problem.


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LAGM2020


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Offlinebaldur
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Re: Making Liquid Culture [Re: mushboy]
    #28057712 - 11/18/22 07:52 AM (1 year, 2 months ago)

Anyone care to comment why LME/BRF/Grain water LC recipies are preferred to f.x. making LC with Karo Corn Syrup?

Just bought some Karo for my first LC test, and just wondering if I should change my strategy.


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OfflineTight Lunchbox
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Re: Making Liquid Culture [Re: baldur] * 2
    #28057886 - 11/18/22 09:29 AM (1 year, 2 months ago)

I think it's better to make due without having to buy extra stuff, that's why I'd just use the lme I normally use for agar if I were to make lc.


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InvisibleShrimps
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Re: Making Liquid Culture [Re: Tight Lunchbox]
    #28057907 - 11/18/22 09:35 AM (1 year, 2 months ago)

Quote:

Tight Lunchbox said:
I think it's better to make due without having to buy extra stuff




One of the best advice in cultivating <3


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InvisiblemushboyMDiscord
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Re: Making Liquid Culture [Re: Shrimps] * 2
    #28058173 - 11/18/22 12:33 PM (1 year, 2 months ago)

Karo is basic bitch.

Pure opinion but I’ve never grown an lc I like with karo syrup:snub:


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Offlinebaldur
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Re: Making Liquid Culture [Re: mushboy]
    #28061853 - 11/20/22 02:56 PM (1 year, 2 months ago)

Quote:

mushboy said:
weigh out 1gram of LME(light malt extract..powder!) i like doing ~1g per ~250ml water.





So I only have LME in syrup form, I assume that's usable in this context? Should I modify the weight in some way?

This page talks about simply adding 20% more when working with syrup if the recipe calls for powder:


Edited by baldur (11/20/22 04:34 PM)


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Offlinebaldur
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Re: Making Liquid Culture [Re: baldur]
    #28062040 - 11/20/22 04:53 PM (1 year, 2 months ago)

Also found a source saying that liquid (syrup) LME will create a cloudy broth... anyone care to comment on that before I go on a hunt for the powder form?


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OfflineMRB3
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Re: Making Liquid Culture [Re: baldur]
    #28069613 - 11/25/22 11:42 PM (1 year, 2 months ago)

This might be a little off topic, but making LC has me thinking about it. How can a grain jar be too wet, if my jar of liquid culture is not too wet? Do you get my drift?


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OfflineTheUsualSuspect
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Re: Making Liquid Culture [Re: MRB3]
    #28069711 - 11/26/22 02:46 AM (1 year, 2 months ago)

Quote:

MRB3 said:
This might be a little off topic, but making LC has me thinking about it. How can a grain jar be too wet, if my jar of liquid culture is not too wet? Do you get my drift?




yah I went down that mental rabbit hole, heres what I concluded after posting and reading:

If your culture is not clean, more water = more chance contams will outrun the mycelium.

My other theory that nobody seems to want to confirm/deny/opine is that mycelium eats by depositing metabolites on a food source, then eating the dissolved food. If a food is dry, the metabolites hit it at 100% strength and more food faster = more growth faster where if its wet, since mycelium is super tiny and only produces so much metabolite, wet food dilutes the metabolites so it takes longer to produce mushroom food hence slower growth. Its not slow in a jar of LC because the food just swirls by and the mycelium just eats it instead of the dissolve and eat cycle. Its a personal theory but nobody has debunked it or tried yet.


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InvisibleAyePlusS
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Re: Making Liquid Culture [Re: TheUsualSuspect]
    #28069895 - 11/26/22 09:20 AM (1 year, 2 months ago)

Grain is hard to sterilize

Liquids arent.

There will almost always be a minuscule amount of bacterial endospores that survive the PC cycle when PC’ing grains, if theres excess water these endospores will come out of stasis multiply and spread, they need water to germinate.

Thats why dryer PC’d grain will sit on the shelf almost forever or until it dries all the way out, not enough moisture to germinate endospores.

This is also why your grain doesnt go bad before you use it, too dey for anything to grow on it.


Quote:

My other theory that nobody seems to want to confirm/deny/opine is that mycelium eats by depositing metabolites on a food source, then eating the dissolved food. If a food is dry, the metabolites hit it at 100% strength and more food faster = more growth faster where if its wet, since mycelium is super tiny and only produces so much metabolite, wet food dilutes the metabolites so it takes longer to produce mushroom food hence slower growth. Its not slow in a jar of LC because the food just swirls by and the mycelium just eats it instead of the dissolve and eat cycle. Its a personal theory but nobody has debunked it or tried yet.




nah


Mycelium loves wet grain if the culture is clean and the grain is actually sterile, i’ve used a bag that took on water in the cooldown pc cycle and it went faster than any if the others that didnt

The only reason it can be slow is often over cooked wet grain is burst and slimy so all the nutrient are free in solution, has tons of food freely available, the grain we spawn fully colonized is still only covered on the outside for the first part of spawn run.



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OfflineTheUsualSuspect
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Re: Making Liquid Culture [Re: AyePlus]
    #28070580 - 11/26/22 06:15 PM (1 year, 2 months ago)

:justno:


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OfflineMRB3
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Re: Making Liquid Culture [Re: mushboy]
    #28180137 - 02/09/23 08:31 PM (11 months, 11 days ago)

I have some questions about LC

1. How often, or at all, do you stir with a magnetic stir plate?

2. How can grain be too wet for good mycelium growth, but LC not be too wet?


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InvisiblemushboyMDiscord
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Re: Making Liquid Culture [Re: MRB3]
    #28180150 - 02/09/23 08:40 PM (11 months, 11 days ago)

most people who use a stir plate keep it on 24/7 i believe. but i could be wrong. i just swirl them once a day.

dont really have a good answer for the 2nd question:dead:


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OfflineMRB3
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Re: Making Liquid Culture [Re: mushboy]
    #28180163 - 02/09/23 08:49 PM (11 months, 11 days ago)

Thanks for the response. That was super fast. I only have two stir plates, but I want to make more jars than that. Maybe I should test how often to do it. I was hoping somebody already knew.


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InvisibleMycolorado
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Re: Making Liquid Culture [Re: MRB3]
    #28180168 - 02/09/23 08:53 PM (11 months, 11 days ago)

I only have 1 stir plate and recently made 2 sslc’s , I just rotated them every 8 hours for a few days.  Worked great.


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