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Re: Using Ziplocs (Ziplocks) as Fruiting Chambers (FC) [Re: fahtster]
    #27424886 -

:dwarf:
How did I miss this one? wish I've seen this earlier!
NICE ONE FAHTsTER!


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Re: Using Ziplocs (Ziplocks) as Fruiting Chambers (FC) [Re: WyoMX] * 1
    #27458464 -

WyoMX said:
Here's some ziplock goodies done on a smaller scale. Had some leftover grains from a g2g and had read through this thread so figured what the heck can't hurt to try a bit. Took a regular sandwich bag mixed some coir with the leftover grains and let em colonize. Cased with jiffy right from the bag and here are my results. From some grains that woulda been thrown away I'll take this everyday and twice on Sunday.





Thanks for the idea to do this Mr faht!
Think I'm gonna do it with a whole qt just to screw around and see what I can get out of 1 qt.


Tryin out the sandwich baggie :thumbup:  PE/RW F2


Faht

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Re: Using Ziplocs (Ziplocks) as Fruiting Chambers (FC) [Re: fahtster]
    #27458482 -

wish i saw this thread earlier! just spawned some iffy stuff to shoeboxes. i wanted to save my shoeboxes for my better jars but didnt really know what to use so i put 2qts in 1 box. i coulda put each qt in a ziplock :facepalm3:

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Re: Using Ziplocs (Ziplocks) as Fruiting Chambers (FC) [Re: Ora]
    #27485017 -

Thanks for the tek! Had some spawn I wasnt super happy with so I gave it a go, plus Im running low on bins!


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Check your vector

Gummies by milkboy
Milkboys Public Display of Affection
I'm a Semp


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Re: Using Ziplocs (Ziplocks) as Fruiting Chambers (FC) [Re: milkboy]
    #27485599 -

I really like the look of this.

You ever tried this with any gourmets? I’ve got some spawn that’s been sat around for far too long but I don’t want to waste it but I’m pretty sure it’s sub prime. I might give pasteurised saw dust a whirl with some oysters for shits and…gonna have a load of atl spawn too so maybe I’ll try and get some of those to fruit (finally) with this…all inspiring dude nice one :thumbup:


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Back once again with the ill behaviour



Links I like

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Re: Using Ziplocs (Ziplocks) as Fruiting Chambers (FC) [Re: Smoothcat]
    #27485784 -

Thought I updated this thread… the baggies are great and so easy.. much easier than the tall 1/2 pint water testers I was doing



The baggies are gonna be my go to for testers from here out

Revived this PE/pfrs f1 clone culture from dried myc grain..


…And made another 2.5 gallon ziplock to see if I can replicate the results



Faht

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Re: Using Ziplocs (Ziplocks) as Fruiting Chambers (FC) [Re: fahtster]
    #27485897 -

Are the 2 gallon size zips the preferred for this Faht?


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PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear


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Re: Using Ziplocs (Ziplocks) as Fruiting Chambers (FC) [Re: PitcherCrab] * 1
    #27486048 -

I like the slightly bigger 2.5 because of the extra room but the 2 gallon work too

Faht

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Re: Using Ziplocs (Ziplocks) as Fruiting Chambers (FC) [Re: fahtster]
    #27494274 -

Doing the 2” PE top layer in a couple 8 cup containers in a lrg zip (1 lined, 1 not) and a couple PE/RW F2 cakes


PE/apeu (papu) f2 baggie 2nd flush coming in.. took a .3 micro dose of these guys today and definitely felted buzzed so that’s a good sign


Faht

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Re: Using Ziplocs (Ziplocks) as Fruiting Chambers (FC) [Re: fahtster] * 2
    #27498601 -

The baggies could be a good way to find a non-blobbing PE culture


Might have to clone that guy and test ‘er out

Faht

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Re: Using Ziplocs (Ziplocks) as Fruiting Chambers (FC) [Re: fahtster]
    #27502613 -

The PE/pfrs f1 clone zip is doing well


These guys break their veil right away and then balloon up.. this is the glc baggie grow of that culture


Clone came from this f1 tub back in February and was dried out on grain and resurrected last month


Faht

Edited by fahtster (10/13/21 12:23 AM)

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Re: Using Ziplocs (Ziplocks) as Fruiting Chambers (FC) [Re: fahtster]
    #27506569 -

My first go... Forgot to label it, gonna have to wait for fruit to see what it was :crazy2:



I was a little worried about sub depth and I may have over filled it because it didn't fit well in the 8 cup tub.

Going to do 3 more today, will go a little easier on the sub volume.


Side note-
I'm in the middle of my first real trich attack, and I'm trying to reduce variables. Lost 4 tubs and counting this week (not bag tek!).

Have you tried hydrating the sub in the bag? Do you reckon that might work?

I'm worried I might have spread trich during spawning (I was doing a few, all the tubs hit so far we're spawned the same day). Wondering if micro batching substrate could help..


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_______________________
For personal religious use only. Embrace infinity for a healthier mind.

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Re: Using Ziplocs (Ziplocks) as Fruiting Chambers (FC) [Re: bagga]
    #27506627 -

Cool!

I don’t think it matters too much about the over filling… I’ve had them kind of muffin top a bit and didn’t seem to make a difference.. if anything, it was better having more CV for water retention.

When you say hydrating the sub in the bag, do you mean after first flush?  Yes, I’ve done that and worked fine.. I’ve also used a smaller 1 gallon bag that didn’t have holes in it to rehydrate.. also worked well.

Sucks about the trich, man.  Have you tried mixing up your coir brands or using boiling water to hydrate if you use hot tap water.  I’m sure you know that most infections happen when making the spawn.. are you g2g’ing?  If so, I’d look there and maybe choose another route.. that’s what my problem was when I got hit hard.. started using glc and the problem went away.  Are you using a SAB or a FH?

Faht

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Re: Using Ziplocs (Ziplocks) as Fruiting Chambers (FC) [Re: fahtster]
    #27506656 -

Thanks for the tek faht
Had a few sketchy jars i was on the fence about so tried out the ziplock tek

(After 2 weeks)


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Check your vector

Gummies by milkboy
Milkboys Public Display of Affection
I'm a Semp


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Re: Using Ziplocs (Ziplocks) as Fruiting Chambers (FC) [Re: milkboy]
    #27506675 -

Awesome!  Glad you found it useful.. lookin good :thumbup:

Forgot to update this thread with the PE/pfrs f1 clone final


The dry weight on that was 29.7g. Not mind blowing but in the realm of keeping the culture of nothing better pops up

The glc grain baggie sub for that culture 2nd flush


This PE baggie did awesome.. 68.8 wet grams (probably dry down to 5-6 which is double what I normally get)


Faht

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Re: Using Ziplocs (Ziplocks) as Fruiting Chambers (FC) [Re: fahtster]
    #27507567 -

Those pe chonkers are great! Crazy to see em in such a small baggy.

Re: GLC
Why do you glc? I read about it, but wasn't sure the advantage over gtg for small batch. Less contam risk during innoc?

Re: hydrating
I was thinking measure the sub dry into each bag and hydrate in the bag.. but thinking about it more that's nuts 😂

I'm using an sab, 8oz and 16oz gtg starters (agar> starter > gtg). I like to have few varieties going at a time, but each is small (which is why your baggy tek is great, shoeboxes were too big). I have a few lcs sitting around, but trying to find the courage to use em😜

Re:spawning
When you spawn different jars, do you sanitize your hands/spoons between each jar? How worried about spawn to spawn contam should I be? I was pretty casual, bit of iso whatever.


I had 40+ grows without trich, moved house.... lost at least 4 of the first 10. Something is up, new space is much dirtier. Maybe my sterile game isn't what I thought it was. Maybe gtg is the vector? My sab is very clean (I test it), but maybe the jars themselves?

Weird thing is that only 3 tat varieties were hit (new Ms grows). None of the clones I did at the same time/same way are affected (so far, they're fruiting).

Thanks again!


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_______________________
For personal religious use only. Embrace infinity for a healthier mind.

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Re: Using Ziplocs (Ziplocks) as Fruiting Chambers (FC) [Re: bagga] * 2
    #27507994 -

Glc:  it’s fast and provides an added layer culture testing for contamination before the culture hits qts.  I shake the jars like 3-4 times when starting from spore… if the jars don’t recover well, I have a bacteria problem and trich will show itself then.  I also clone straight to grain so glc is just naturally the next step for me.. yesterday I GLC’d a Gandalf and PE/apeu clone to qts and the cultures looked amazing


There is, of course, risk when using SHIPs but it’s not as bad as one might think.. I lose maybe 1 in 30 qts because of a dirty poke but it’s way less riskier than opening a jar in a SAB as I believe you’re finding out… I go to great lengths to make sure my SHIPs are as clean as possible before I inoculate.. I first wipe with H2o2 and let the soaked wipe sit on the SHIP before I go to using iso wipes.. I use large SHIPs and actually bend down so the light can show me if I’m about to poke into any remaining iso droplets :lol:

G2g’s greatest pro is also its greatest con, IMO.. it’s fast.. too fast.  When you g2g, the jars colonize very quickly but opening the jar is a HUGE contam vector.. all you need is one tiny particle of dust or mold spore that finds its way into one of the two giant openings in either jar and you’re fucked and because the jars colonize so fast, that infection could be hidden in the middle of the jar… add the one jar to a 66qt bin and you won’t see that you’re fucked until riiiiight before it’s about to pin

Glc is fast but it still takes 4-5 days to get to the point of a shake even when using 5ml of inoculate.. if I have an infected jar, I’ll see that infection after the shake and I won’t spawn it, obviously.  You won’t see me post a moldy tub.. I haven’t had one in the last 3 years since I started back because of all the failsafes I have getting to the point of spawning (now watch me lose a bunch of tubs because o said that :lol:).  I also use smaller tubs so in case I do lose a tub, I’m out a couple jars instead of 4-5.

I go to great lengths to make sure I have clean cultures.. This is my printing technique that allows me to safely go spores straight to grain and This is my cloning technique, so I’m pretty meticulous.  Those lids also allow me to dry out the culture both for storage and if the culture is slightly bacterial.. drying them out knock backs bacteria (at least bacteria that doesn’t produce endospores, which is most of them)… my printing method doesn’t guarantee clean spores to grain but it’ll usually be enough that I can, at the very least grow out a baggie of spawn and get clone material to get some clean glc going.. so all these things come together to make sure I have clean inoculate before I even send it to qts

I’m pretty small time compared to a lot of folks, maybe yourself included, so my methods are perfect for the amount of tubs I run at a time (10-15 sixteen qt bins) and it’s not lost on me that my methods wouldn’t make sense for someone running 30+ 66qt tubs.. in that case, I’d get an ffu and run bag to bag inoculations

Sounds to me like your new place has a higher mold load than your last (maybe there’s some mold somewhere you’re not aware of.. air ducts etc. (also the reason I take the summers off) and you’re introducing infection during g2g (I’ve been there.. I lost 12+ 54qt bins just before pinning years ago which prompted me to totally revamp my methods).

If you’re looking to make small batches of CV substrate, check out This link… that’ll help :thumbup:

Faht

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Re: Using Ziplocs (Ziplocks) as Fruiting Chambers (FC) [Re: fahtster]
    #27508189 -

Love your work faht, enjoyed that post.


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Willpower is the one true virtue


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Re: Using Ziplocs (Ziplocks) as Fruiting Chambers (FC) [Re: smalltalk_canceled]
    #27508220 -

Thanks man, I appreciate the props!  I dig your exotics work :thumbup:

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Re: Using Ziplocs (Ziplocks) as Fruiting Chambers (FC) [Re: fahtster] * 1
    #27515379 -

Making a bunch of the 2.5 gallon 1qt spawn ziplock containers.. here’s the first five


Faht

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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