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PitcherCrab
Crescent Fresh



Registered: 12/02/06
Posts: 2,446
Loc: The bottom of the sea.
Last seen: 1 year, 1 month
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
#27130713 - 01/06/21 02:45 PM (3 years, 22 days ago) |
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1/6/2021
Update, I just plated some Melmac and PE, because why not. I've never grown PE and figured it was about time. Also I just got some germination on a plate of TOC, but it was one that I had swabbed 12/7/20, so not technically in 2021.

Updated original post as well here: https://www.shroomery.org/forums/showflat.php/Number/27125101#27125101
-------------------- PC's LAGM 2021 (TOC & TWC)P. natalensis Growlog 2021Pans for PC Fall 2021 Growlog “I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear
Edited by PitcherCrab (01/06/21 03:44 PM)
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BrownBear
Warrior-Traveler



Registered: 06/05/20
Posts: 1,539
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: PitcherCrab] 3
#27130751 - 01/06/21 03:07 PM (3 years, 22 days ago) |
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I have lift off on my rustywhyte plates. 5 days to germination is damn good.
Those complaining about plates showing no germination after 5 days. It is typical for spores to take 7-10 days to germinate. Depending on how old the print is, it could take even longer. I would suggest giving it atleast another 2 days.
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: BrownBear]
#27130772 - 01/06/21 03:13 PM (3 years, 22 days ago) |
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Bear - that's solid advice. I'll quit complaining and try to be more patient lol. I have all the patience in the world waiting for grain to colonize but I apparently have very little with plates..
Roar - if I don't see any activity in a few days I'll hit you up. Thanks!
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I 5318008 NOT a virgin!
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 2 years, 10 months
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So not actually part of the LAGM grow but thought you all may enjoy.
I know I'm not the first and they sell ready made jars and lids and tubes for this. I in fact have those attachments somewhere;no clue where. So I threw this together to A: serve my purpose B: share the design\idea for others
In this instance I'm using a vacuum sealer but I have plans and will be making a diy vacuum pump in the near future I can share as well. So even withouy a vacuum sealer you can vacuum hydrate cakes (and other uses). Running some tests and weighing everything. I will eventually add this as part 3 of my Doing "science" series in my sig but here's a quick pic or two for now.

You can see at first the cake floats as it is full of air. In the second it has sank after two 10 second vacuum sessions with 30-60 seconds rest between. Fuck 24 hour soaks.
Edited by GypsyBastard (01/06/21 03:35 PM)
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RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
#27130857 - 01/06/21 03:53 PM (3 years, 22 days ago) |
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I’ve got germination on my APE and Melmac, but only where I jammed the swab into the agar. My streaks haven’t taken off yet.
Some nice myc off the Melmac swab.

Just the tiniest bit of white on the dark background of spores on the APE swab.
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ReverendMyc

Registered: 03/29/19
Posts: 1,496
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
#27130863 - 01/06/21 03:55 PM (3 years, 22 days ago) |
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MuthaFudpucker Grows some stuff (update)2021-01-06 Got some more germination action on the Tamps and now a tiny start on the PE. Also a contam next to the best germ so far on plate 102, but I think I'll wait for a transfer to see if I can get some more isolated germination from further down the loop streak. I am just happy to see any PE germination from a syringe that has been in the door of my beer fridge for a year.  EDIT: to say that I guess that Tamps are a really fast germinator and these are a fresh print, so they are probably not a fair comparison to Cubes. Waiting two weeks and more is not unheard of at all for cube spores to germinate.
-------------------- LAGM 2.024Stoned Gummys tek (Gummies from sclerotia or mushrooms) *Not just for stones any moreHow to succeed in mycology (and life) - know nothing, read everything, try something, and accept advice. Don't Panic   
Edited by ReverendMyc (01/06/21 04:07 PM)
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Doctor Mario
🍄🍄🍄🍄🍄



Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: ReverendMyc]
#27131028 - 01/06/21 04:55 PM (3 years, 22 days ago) |
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I'm enjoying everybody's logs. Keep em coming.
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Madscience039
Yodeler

Registered: 07/06/20
Posts: 128
Last seen: 2 years, 8 months
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Doctor Mario]
#27131058 - 01/06/21 05:06 PM (3 years, 22 days ago) |
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TAT Black Cap germination
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Tankie_J
I like having fun



Registered: 01/09/19
Posts: 537
Last seen: 10 months, 13 days
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Tankie_J]
#27131059 - 01/06/21 05:06 PM (3 years, 22 days ago) |
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Quote:
Tankie_J said:
Species: P. Baeocystis
-02JAN21- 2 lme plates via scraping the gills with a sterile blade onto the agar. I used the blade to push around some of the material on the agar. Donor is an urban harvested fruit out of the Seattle area. Pic shows dried fruit on top of the unopened maz print

-04JAN- Something has germinated. Both plates have an identical spot of something mysterious and it’s fucking fast.

-05JAN- The growth has shot spores... 

-06JAN- Tossed both plates, there’s no saving that mess. Not sure if I will try again. Hindsight: Should have taken prints after I found them.
I have some new prints on the way, one of them will replace this grow.
Edited by Tankie_J (01/06/21 05:23 PM)
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Melliferous
🌵🍄🌵🍄🌵



Registered: 10/01/20
Posts: 1,053
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Tankie_J]
#27131066 - 01/06/21 05:10 PM (3 years, 22 days ago) |
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Did your plates contam out Tammie?
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      "No, I don't worry. I tell you, I am a man who believed that I died 20 years ago, and I live like a man who is dead already. I have no fear, whatsoever, of anybody or anything."
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MycoBrainz
Mycotic



Registered: 03/14/15
Posts: 615
Loc: West Coast
Last seen: 4 months, 10 days
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Melliferous]
#27131072 - 01/06/21 05:14 PM (3 years, 22 days ago) |
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Hey guys, What exactly am I looking for when transferring? Kinda new to the whole isolation of strains. I have lots of growth but where to start?
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PFC x Creeper Lets Get Stoned
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Melliferous
🌵🍄🌵🍄🌵



Registered: 10/01/20
Posts: 1,053
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: MycoBrainz]
#27131076 - 01/06/21 05:16 PM (3 years, 22 days ago) |
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Quote:
MycoBrainz said: Hey guys, What exactly am I looking for when transferring? Kinda new to the whole isolation of strains. I have lots of growth but where to start?
A clean leading edge to help ensure a clean culture without hidden contaminants. Also select for myc vigor and health. I've had both tomentose and rhizomorphic mycelium fruit well so in my view there should not be a preference for either.
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      "No, I don't worry. I tell you, I am a man who believed that I died 20 years ago, and I live like a man who is dead already. I have no fear, whatsoever, of anybody or anything."
Edited by Melliferous (01/06/21 05:17 PM)
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Ballzagna
Carb Daddy



Registered: 10/09/19
Posts: 619
Last seen: 13 days, 11 hours
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: MycoBrainz]
#27131084 - 01/06/21 05:20 PM (3 years, 22 days ago) |
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-------------------- No one likes a naysayer It's probably fine. Even if it's not fine, it'll eventually be fine. So it's fine. PE Adventure
Photosynthesis: A Morning Ritual Ballzagna's Tek Compendium and Notes for Noobs 
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MycoBrainz
Mycotic



Registered: 03/14/15
Posts: 615
Loc: West Coast
Last seen: 4 months, 10 days
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Ballzagna]
#27131108 - 01/06/21 05:30 PM (3 years, 22 days ago) |
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Thanks, I was just reading through RR's posts on isolation.
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PFC x Creeper Lets Get Stoned
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Doctor Mario
🍄🍄🍄🍄🍄



Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Ballzagna]
#27131110 - 01/06/21 05:30 PM (3 years, 22 days ago) |
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I'm still waiting to make sure I've got clean plates to put spores on. Just checked and saw that 20/34 are germinating mold. Whatever it is, its very wispy, has long strands and is very fast. I usually have a few that contam after pouring in the SAB but this is excessive as hell. The company I've been buying my plates from switched what they were selling. Usually, I get falcons that are high quality and come in a thick plastic sheet that you peel away when you open, similar to a syringe package. These new plates came in this...

Makes me wonder if they were even sterile to begin with. Even if they were, I doubt that thin ass plastic holds up very well while handling. These don't qualify because the myc was already on grain when entering this LAGM but here's some RW shoeboxes that are pinning and a RW unmodified tub that is still colonizing. (The coir in the tub is a lot darker then the picture shows. Not sure why but my phone wants it to appear light brown. I assure you, the coir is indeed at field capacity.)
 
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Tankie_J
I like having fun



Registered: 01/09/19
Posts: 537
Last seen: 10 months, 13 days
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Melliferous]
#27131114 - 01/06/21 05:33 PM (3 years, 22 days ago) |
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Quote:
Melliferous said: Did your plates contam out Tammie?
So fucking funny that tankie auto corrects to tammie...
Yeah the Baeos shot some nasty spores. No germ on the pans or maz yet. I have a shit show of a mess with a bunch of APEs, I think the LC I got is a bad culture, not the most reliable source. I have a different APE culture on grains from a swab from Roarkell, it’s just going slow. ‘Nocd up on Dec26 and there’s about a quarter sized growth area. I will be reaching out for advice once I spawn them.
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roarkell
The Roar Master!



Registered: 08/29/19
Posts: 2,223
Loc: Southern WI
Last seen: 6 months, 21 days
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Tankie_J]
#27131148 - 01/06/21 05:43 PM (3 years, 22 days ago) |
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APE can be pretty slow sometimes... I don't have much advice on speeding them up either... lol
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Melliferous
🌵🍄🌵🍄🌵



Registered: 10/01/20
Posts: 1,053
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Tankie_J]
#27131178 - 01/06/21 05:53 PM (3 years, 22 days ago) |
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Quote:
Tankie_J said:
Quote:
Melliferous said: Did your plates contam out Tammie?
So fucking funny that tankie auto corrects to tammie...
Yeah the Baeos shot some nasty spores. No germ on the pans or maz yet. I have a shit show of a mess with a bunch of APEs, I think the LC I got is a bad culture, not the most reliable source. I have a different APE culture on grains from a swab from Roarkell, it’s just going slow. ‘Nocd up on Dec26 and there’s about a quarter sized growth area. I will be reaching out for advice once I spawn them.
Nah, I watched that whole exchange go down, that wasn't autocorrect. 
Hopefully your other plates succeed!
--------------------
      "No, I don't worry. I tell you, I am a man who believed that I died 20 years ago, and I live like a man who is dead already. I have no fear, whatsoever, of anybody or anything."
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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: roarkell]
#27131209 - 01/06/21 06:04 PM (3 years, 22 days ago) |
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Quote:
roarkell said: APE can be pretty slow sometimes... I don't have much advice on speeding them up either... lol 
It takes months to narrow down a fast growth of ape. Even then it's terribly slow. Especially with fruiting.
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roarkell
The Roar Master!



Registered: 08/29/19
Posts: 2,223
Loc: Southern WI
Last seen: 6 months, 21 days
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: roarkell]
#27131380 - 01/06/21 07:05 PM (3 years, 22 days ago) |
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Quote:
06DEC2021: The swab dunk on the SAT threw me off, thought I had some growth but its just mold...  Nothing on the gyms either... Pic dump of the rest of the plates im taking transfers from.
 The seventh pic is the only PDA plate to harbor life... but that Fiji was a few days ahead of the rest so that may have something to do with it...
Updated OP... gonna get these transfers knocked out and then I need some sleep...
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