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Offlineredbee
prole spore threat
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Registered: 03/30/20
Posts: 175
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: redbee] * 1
    #27123823 - 01/03/21 12:35 PM (3 years, 25 days ago)

Got a late start, but here's my first entry in the log, quoted below.
Full post is here, with some extra info about the grow.

GypsyBastard, thanks for adding me (and for running this)!

Quote:

redbee said:

JANUARY 03, 2021



Starting with some plates I’d poured and PC'd over the holidays. These are agar : LME at a 10 : 7.5 ratio, or so, and poured as thin as possible. I prepped the agar ages ago—I did a huge batch and stored it in several pyrex bottles kept in the fridge, and I've just been reheating and prepping small batches of no-pours as needed. It's nice to not have to cook up agar every time I need to pour some plates.



I’ve only been doing sterile work since September, and it’s a testament to my growth that it doesn’t feel like nerve-wracking misery anymore. When I first started, I thought I’d never feel comfortable. I definitely still have spaz-moments, but the majority of the time I can slip into a flow-state pretty easily.

Today was full of spaz-moments, though. I was inoculating with swabs, which I’m always kinda ham-fisted with... I just don't trust that they'll transfer spores?! I usually just streak them, but I saw someone else’s LAGM post where they talked about using their exact-o to trim some cotton fuzz and directly drop that on the agar. Well... TIL, easier said than done. I had a nightmare time with it because my blade was dull and rusted from a couple of runs through the PC and innumerable torchings. Hindsight: I should have swapped for a fresh one. I shredded the cotton on the swab tips, and when I finally got some wispy threads onto my blade, I proceeded to shred my agar while trying to remove the cotton from the blade. The fibers just did not want to jump from the jagged-edged blade onto the smooth surface of the agar...weird, huh? :lol:



Here’s what I nocc’d up:

APEU:
Swab Plate A: streaked with swab
Swab Plate B: streaked with swab
Swab Plate C: shredded cotton strands
Swab Plate D: shredded cotton strands

Rusty Whyte:
Swab Plate A: shredded cotton strands
Swab Plate B: streaked with swab
Swab Plate C: cut the swab tip and dropped it onto the plate

We’ll see which ones colonize first. I’d be surprised if the cotton-strand plates don’t show contam, based on how long I had to keep them open in order to dislodge the fibers.







--------------------
:sporedrop:  redbee's LAGM 2021  :sporedrop:


Edited by redbee (01/03/21 12:48 PM)


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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: bigfootscreepyuncl]
    #27123883 - 01/03/21 12:58 PM (3 years, 25 days ago)

Luckily the only swabs I have are ones that I made a year or so ago from a mss. They were part of a trade or giveaway and I sent the wrong ones by mistake then lost these. So finding them was a total bonus and I was a bit careless with them. One swab per plate and just used them like a loop then threw them aside. Still have a at least one each of them left so if these don't germ I'll be a bit more resourceful with the remaining swabs. The Coast Ricans were about average on my one grow; nothing notable good or bad. The Puerto Ricans however, were weird as fuck! Really strange growth, tons of mutants... some grow flat, some ballooned and never opened. But a SUPER dense flush and decent potency. So I am kinda excited to play with those again.

*Disclaimer: There a chance my memory is fucking with me and its the CR that were really weird so I am bringing both back. I'm fairly certain I remember these correctly though.


redbee- Glad to have you with us! :smile: Awesome log entry!


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OfflineA.k.aM
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Registered: 10/27/19
Posts: 16,782
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: redbee]
    #27123897 - 01/03/21 01:02 PM (3 years, 25 days ago)

Get at it sock, I haven’t started either.

Last year I’m pretty sure people were still jumping in during summer.



According to my notifications I’ll have my case tomorrow finally. Sooo many prints to streak!


Especially excited for necD and p+. And Gandalf. ....and twc....:facepalm:


--------------------
LAGM2020


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OfflineMycoBrainz
Mycotic
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Registered: 03/14/15
Posts: 615
Loc: West Coast
Last seen: 4 months, 10 days
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: bigfootscreepyuncl]
    #27124101 - 01/03/21 02:39 PM (3 years, 25 days ago)

Quote:

MycoBrainz said:
I'm in. Greenhouse got shutdown. I'll have some time. Just poured 75 plates.

Streaked X7X and Moby Dick 1/3/2021






Getting ready to pour 100 more plates today.  Made Green, Blue,& Purple MEA. My streaks starting to take off. Especially the pan cyan Aussie, Hi and Al.


Edited by MycoBrainz (01/16/21 11:59 PM)


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Invisibleallthespores


Registered: 12/14/20
Posts: 42
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: A.k.a]
    #27124139 - 01/03/21 02:53 PM (3 years, 25 days ago)

the swamp shows first signs of growth on 2 plates.
if this fails, i have looked into some tek to avoid this mess.
leaving them inside the PC until cooled down caused it.



--------------------
LAGM2021 Grow log


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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: allthespores]
    #27124143 - 01/03/21 02:56 PM (3 years, 25 days ago)

Got you updated in the OP Sockadin. Welcome aboard!!


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OfflineTankie_J
I like having fun
I'm a teapot


Registered: 01/09/19
Posts: 537
Last seen: 10 months, 13 days
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: MycoBrainz]
    #27124155 - 01/03/21 03:05 PM (3 years, 25 days ago)

I found a RW f13 while digging through the catalog. I’m thinking maybe try an APE/RW cross too for shits and giggles.

I haven’t searched on this yet, but how are you people doing multicolored agar? I tried a couple times by just adding a bunch of food coloring and the color faded after PC. Could of been the plant based food coloring I had on hand.


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OfflineEugene Gesuale
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Registered: 04/12/20
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Tankie_J]
    #27124178 - 01/03/21 03:17 PM (3 years, 25 days ago)


What do you guys think of this size container for CVG spawned at 1:1. How thick should I make the sub. It’s not a pe variant


--------------------
Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.


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OfflineTankie_J
I like having fun
I'm a teapot


Registered: 01/09/19
Posts: 537
Last seen: 10 months, 13 days
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Eugene Gesuale]
    #27124226 - 01/03/21 03:53 PM (3 years, 25 days ago)

These things will grow just about anywhere, but I’d say it’s good for prepping the sub, not growing in. Also a 1:1 ratio might produce some mutants, might be a little heavy on the spawn, but that’s what this thread is about, taking gambles and putting it out there. Def take all my advice with a grain of salt as I am still learning as well.


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Invisiblealaskappalachian
Entitiologist

Registered: 10/22/19
Posts: 1,674
Loc: The 49th Dimension
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Tankie_J]
    #27124256 - 01/03/21 04:10 PM (3 years, 25 days ago)

Yeah likely the plant-based stuff is the issue.  I think I've read that before,  actually.  Just about everyone uses McCormick or charcoal.


--------------------


"First we build the tools, then they build us."

THE 49th MYCOJOURNAL: Exotics, Auroras, and Entities


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OfflineTight Lunchbox
Drunk cat


Registered: 11/06/16
Posts: 2,116
Last seen: 4 months, 7 days
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Tight Lunchbox]
    #27124265 - 01/03/21 04:16 PM (3 years, 25 days ago)

Quote:

Tight Lunchbox said:
2021 LAGM


January 3

Today I finally got around to pouring some MEA for some Nepal Chitwan spores. I chose this variety simply because it was the freshest print I had at my disposal. The print was part of a trade with some rando awhile back so we'll see how dirty it is. I ended up swiping spores on 10 plates with an inoculation loop.




It's worth mentioning that I forgot to tighten the lid on my media bottle for like 20 or 30 minutes after taking the cover off my PC to let everything cool down. I'm eager to see if I end up with a bunch of contaminated plates.




--------------------
"it's all a joke between mom contractions and coffin fittings"


The most useful tool for noobs


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OfflineTankie_J
I like having fun
I'm a teapot


Registered: 01/09/19
Posts: 537
Last seen: 10 months, 13 days
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Tight Lunchbox]
    #27124322 - 01/03/21 04:50 PM (3 years, 25 days ago)

Quote:

alaskappalachian said:
Yeah likely the plant-based stuff is the issue.  I think I've read that before,  actually.  Just about everyone uses McCormick or charcoal.




Right on, I have some kroger neon on hand now so I will go with those and give it another shot

Quote:

Tight Lunchbox said:

It's worth mentioning that I forgot to tighten the lid on my media bottle for like 20 or 30 minutes after taking the cover off my PC to let everything cool down. I'm eager to see if I end up with a bunch of contaminated plates.




I think there’s a prize for most colorful tammed plate, good luck!  :douchewink:


Edited by Tankie_J (01/03/21 04:55 PM)


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InvisibleWonko
the Insane
Registered: 10/02/20
Posts: 7
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
    #27124345 - 01/03/21 05:00 PM (3 years, 25 days ago)

Wonko's LAGM 2021 Grow Log


Species: P. Cubensis
Variant: Brazilian, B+


Notes:

I'm relatively new to cultivation, having started this past August.  Had some moderate success with a MSS to brown rice grow and slightly better results with a MSS > agar (no pour) > WBS grow.

This will be my first attempt with poured agar.  I will be going MSS to agar.

TEKs:

Sterile: Just a SAB and copious amounts of 70% ISO.

Agar: Mostly followed Bod's Comprehensive TEK. Recipe is 10g agar-agar, 10g LME, and 500ml water, PC'd @ 15 PSI for 20 minutes. Used condiment cups instead of petris for this attempt. I think the pour was done a little thick.  I will try to get them thinner next time.

Grain: Will be hamloaf's no-soak WBS.

S2B: Will (hopefully!) be to shoeboxes following ShaperDreaming's SFF Shoebox TEK.


01/03/2021 Update:


MSS to Agar:

Prepped SAB, flame-sterilized syringe, squirted spore solution into an oven-sterilized shot glass.

Flame-sterilized inoculation loop, cooled loop in agar cup, dipped loop in spore solution, streaked agar with loop. Repeated process for each cup.



Inoculated 8 cups in total. Now we wait!

01/11/2021 Update:


Contaminated!

Well, all 8 of these cups ended up contaminated. 

Will have to postpone this grow for a while as I have an unexpected house guest for the next couple of weeks.

May switch to a different MSS when I resume.

01/28/2021 Update:


Starting Over:

Finally got to start over after the last contam-fest. Streaked 6 more plates today; 3 - Brazilian and 3 - B+, both from MSS:



Jars in the background are from a cloning project. Hope to be shaking those soon!


Edited by Wonko (01/28/21 04:55 PM)


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OfflineTight Lunchbox
Drunk cat


Registered: 11/06/16
Posts: 2,116
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Wonko]
    #27124355 - 01/03/21 05:10 PM (3 years, 25 days ago)

I think it's fairly relaxed aside from the only rule being starting spore to agar. I might be wrong though. Don't forget to post pics.


--------------------
"it's all a joke between mom contractions and coffin fittings"


The most useful tool for noobs


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OfflineInthepit
Aum Mani Padme Hum
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Registered: 08/20/19
Posts: 1,742
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: redbee] * 1
    #27124381 - 01/03/21 05:28 PM (3 years, 25 days ago)

Great data redbee! Keep it up!
And hey Sockadin glad to to see you on board!
And oh, AKA you da man!


Edited by Inthepit (01/03/21 05:34 PM)


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OfflineGypsyBastard
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Registered: 11/30/20
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Inthepit]
    #27124450 - 01/03/21 06:09 PM (3 years, 25 days ago)

Unfortunately there IS a entry deadline. We can only allow new participants up until 12/31/2021 (31/12/2021 for those across the pond). :lol: Luckily, you just squeaked under the bar, Wonko. Glad you made it!! Another 362 days and you would have missed it.


Edited by GypsyBastard (01/03/21 06:14 PM)


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InvisibleWonko
the Insane
Registered: 10/02/20
Posts: 7
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
    #27124493 - 01/03/21 06:31 PM (3 years, 25 days ago)

Quote:

GypsyBastard said:
Unfortunately there IS a entry deadline. We can only allow new participants up until 12/31/2021 (31/12/2021 for those across the pond). :lol: Luckily, you just squeaked under the bar, Wonko. Glad you made it!! Another 362 days and you would have missed it.





Whew, good to know.  Next time I will procrastinate more! :dancer:

Thanks for the add.


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OfflineEugene Gesuale
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Wonko]
    #27124512 - 01/03/21 06:40 PM (3 years, 25 days ago)

Hey gypsy. I’m growing PESA! Spores to agar before the new year, although I would like to participate in this grow along


One ms plate started 12/15 and three separate T1s from it started 12/29.

I took a wedge from each T2 to its own quart of oats on 1/6
They are almost microscopic so hopefully they leap off well
Also, don’t worry , the jar on the right isn’t all tammed up, that’s some shit I just scraped off with my thumbnail on the outside of the jar 😅


and started 2 new plates one with APE/TOC and AveryA/PESA


I spawned one quart oats to 3 quarts coir on 2/4/21 PESA APE TOC 
And started 3 new spore plates.
Playa
Nats
And one I will call PUNTS if I get those two to cross


--------------------
Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.


Edited by Eugene Gesuale (02/05/21 10:15 AM)


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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Eugene Gesuale]
    #27124591 - 01/03/21 07:27 PM (3 years, 25 days ago)

I gotcha adrenochrome. Un momento :smile:


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OfflineNarrator
Strangest in the night
I'm a teapot User Gallery


Registered: 11/30/20
Posts: 187
Last seen: 1 year, 5 months
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
    #27124674 - 01/03/21 08:16 PM (3 years, 25 days ago)

My APE syringe from a sponsor looks goopy and there's already growth in those dishes, less than 36 hours later. Is it possible the spores germinated in the syringe or am I growing more mold?



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