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Iambrownbear
Warrior-Traveler



Registered: 06/05/20
Posts: 630
Last seen: 9 hours, 33 minutes
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
#27108590 - 12/26/20 11:59 AM (2 months, 9 days ago) |
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Thank you for hosting this thread. I am really excited to see everyone's progression and of course all of the pin porn that will come of it.
-------------------- Brown Bear's Grow Log Adventure SET IT & FORGET IT GROW TENT "Become like water my friend." ― Bruce Lee "The basic difference between an ordinary man and a warrior is that a warrior takes everything as a challenge, while an ordinary man takes everything as a blessing or as a curse." -Carlos Castaneda "A man of knowledge is free...he has no honor, no dignity, no family, no home, no country, but only life to be lived." --don Juan
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 489
Loc: Gamehenge
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Iambrownbear]
#27108645 - 12/26/20 12:21 PM (2 months, 9 days ago) |
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Just got my two 500ml media bottles in the mail!! I'll be pouring plates today or tomorrow to get ready for the spore drop. The real trick is going to be to not get fucked up on New Years eve so I can inoculate before bed since we're having a few neighbors over for New Years brunch and everything needs to be out put away before then. Can't wait to start this log. Cheers, mates!
--------------------
Bigfoots LAGM21
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,394
Loc: Puerto Rico
Last seen: 32 minutes, 57 seconds
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Oh don't worry about New Year's Eve. ( I know it's part of the fun) I didn't get back in the country till Jan 17 last time.
Although for those wondering about starting late, it was cool for me because I was watching everyone go to agar. So I ended reading all about before I did it.
And look at AKA! He's number one already!
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coversall
إِنْ شَاءَ ٱللَهُ


Registered: 06/06/20
Posts: 1,331
Loc: संसार
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Inthepit]
#27108821 - 12/26/20 01:53 PM (2 months, 9 days ago) |
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Quote:
Inthepit said: Oh don't worry about New Year's Eve. ( I know it's part of the fun)
Nioce, my timings may be a little wonky at the start of the year, I will grow mushrooms in 2021, just may not start at the same time as most people in the thread.
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Tight Lunchbox
Drunk cat


Registered: 11/06/16
Posts: 1,229
Last seen: 1 hour, 2 minutes
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: coversall]
#27108827 - 12/26/20 01:55 PM (2 months, 9 days ago) |
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2021 LAGM
January 3
Today I finally got around to pouring some MEA for some Nepal Chitwan spores. I chose this variety simply because it was the freshest print I had at my disposal. The print was part of a trade with some rando awhile back so we'll see how dirty it is. I ended up swiping spores on 10 plates with an inoculation loop.
 It's worth mentioning that I forgot to tighten the lid on my media bottle for like 20 or 30 minutes after taking the cover off my PC to let everything cool down. I'm eager to see if I end up with a bunch of contaminated plates. January 11One plate is showing a few spots of germination, will update in the next few days with pics. January 21It looks like I have some shit riding along with my chitwan mycelium on the t1 plates from the 11th, and I don't yet know how I'm going to proceed.   January 22I did swabs on the print and buried them in some plates, also took some transfers from the shitty t1 plates. The growth I've been observing really doesn't look like I can do anything with it so I'm probably wasting my time. There was an ok looking germ plate that I might be able to get something from, so I did a few transfers from it. March 5Here is one of four or five t2 plates from that germ plate I previously went back to. The growth looks ok enough, so I'm going to noc up 6 jars and do some transfers.  I'm still really bad at taking plate pictures. The condensation on the lid doesn't help any. Right now some wheat is being prepared so the jars should be inoculated some time tomorrow morning.
Edited by Tight Lunchbox (03/05/21 02:32 PM)
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Josex
#cheat_mode


Registered: 11/13/15
Posts: 7,567
Loc:
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Tight Lunchbox] 1
#27109354 - 12/26/20 06:51 PM (2 months, 9 days ago) |
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Here's how my growlog starts. Will inoculate some plates on January 1st. I'm stoked. 
Quote:
JOSEX' PENIS
26 December.

Took some swabs from this grow:

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GrinchGrower
N00B



Registered: 10/02/20
Posts: 678
Last seen: 1 day, 11 hours
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Josex]
#27109420 - 12/26/20 07:31 PM (2 months, 9 days ago) |
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Quote:
Josex said: Here's how my growlog starts. Will inoculate some plates on January 1st. I'm stoked. 
Quote:
JOSEX' PENIS
26 December.

Took some swabs from this grow:

Lots of options....smart!
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 12 hours, 59 minutes
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Josex]
#27109438 - 12/26/20 07:38 PM (2 months, 9 days ago) |
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Quote:
Josex said: Here's how my growlog starts. Will inoculate some plates on January 1st. I'm stoked. 
Quote:
JOSEX' PENIS
26 December.

Took some swabs from this grow:

cant wait to see your dick pics
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 12 hours, 59 minutes
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
#27109502 - 12/26/20 08:22 PM (2 months, 9 days ago) |
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Quote:
GypsyBastard said:
  *********GypsyBastard presents: LAGM2021 Grow Log**********  
        
Species:
P. Cubensis
Variant:
GT
PFC
Added a bit of the ol razzle dazzle Inspired by Inthepit's log page
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Josex
#cheat_mode


Registered: 11/13/15
Posts: 7,567
Loc:
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
#27109543 - 12/26/20 08:39 PM (2 months, 9 days ago) |
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mushboy
Moooshboy?



Registered: 04/24/05
Posts: 24,789
Loc: better trashcan
Last seen: 12 minutes, 9 seconds
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Josex]
#27109651 - 12/26/20 09:49 PM (2 months, 9 days ago) |
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Narrator
Strangest in the night



Registered: 11/30/20
Posts: 156
Last seen: 10 hours, 57 minutes
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Josex]
#27109711 - 12/26/20 10:27 PM (2 months, 9 days ago) |
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I started my agar at midnight on 1.1.2020. I'm using Telephone brand this time. When pouring, I realized I mistook the old bag of agar for LME. So I waited until it was light out and poured plates of LME agar, some with 2mg honey per 500ml.
Few if any of the agar recipes posted here specify liquid or dry LME. Liquid is more common in the home brewing background I came from and I had to guess that mycologists would work with dry LME.
1.2.2021
4 plates APE MSS 4 plates Puerto Rico MSS 4 plates Pholiota adiposa MSS made from a grocery store specimen.

1.3.2021 Looks like my APE syringe is dirty.

1.4.2021
Noc'd up 4 more plates of APE, dipping a needle in a drop of spore solution and scratching the surface. Also ordered some fish antibiotic to try and plan on doing some Josex style BRF pucks. I want some dick soooo bad! https://www.shroomery.org/forums/showflat.php/Number/24806569/fpart/all/vc/1
1.6.2021
Something interesting looking among the gunk on one of the APE plates.

1.9.2021
Puerto Ricans germed a couple days ago.

Two spots of something white germing on one of the P. adiposa plates.

The first APE plate that looked like it might have mushroom mycelium is looking...someone tell me how it's looking? Anything salvageable? I have Furan-2 to try in my next agar dishes.

Here's what another gunky APE dish looks like.

There might be some growth in this dish with is more uniformly and has less apparent bacterial growth.

While starting a Lions Head project 1.4.2021 I noc'd up one jar of grain with the APE MSS. If all else fails maybe this will allow me to get a spore print to take to agar really late.

1.14.2021

Row 1 APE day 13. The leftmost is the dish is the one that was so colorful recently. Looks like the mycelium is winning the battle.
Row 2 Puerto Rican day 13. Is the largest growth the best to take a transfer from or do I want to prioritize vigor and symmetry?
Row 3 APE day 10. Tried to noc up plates from the contaminated syringe by just getting the tip of a needle wet with spore solution and making scratches in the agar. One point of germination and no nasty bacteria that at least I can see as in the other dishes started from the syringe.
Row 4. APE rescue. I transferred to agar some growth from one of the WBS jar that also had a spot of mold. Hope I get some APE and spores!
Unpictured. P. Adiposa day 13. One small slow growing area of growth that looks to my newbie hopeful eyes more like mushroom than mold. No other germination points. Thinking about starting more spores.
Unpictured. With the last APE spore solution I started a BRF puck. The lid had sealed to the jar in the PC so it sucked the approx 6CC remaining in the syringe. It does not appear to be too wet and has germinated nicely.
Will be making more plates for transfers tomorrow or this weekend!
1.19.2021
Using new batch of LME plates with FURAN-2, 6g LME/8g telephone agar / 500ml distilled H20. Let the dishes site for four days in SAB before using them, very little condensation.
Took T1s from the once beautifully contaminated plate. A slice from where the battle was fought and two from a late germinating spot.

More T1s from other plates. How clean does the plate started 1.4 look?

T1s for the Puerto Ricans.

Giving up on the P. adiposa, perhaps will try again soon but have enough projects started for now.
1.29 APE T2s

1.30 PR T1 progress on day 11. Ready for T2 or not yet?

2.10 Had a pacer put in a few days ago. Behind on transfers!

But a few of my APE t2s look good!
2.22 PR to T3 yesterday.

APE plates are one transfer ahead.

2.26.2021 The APE T4 plates had too much condensation to photograph. I was sloppy on both Puerto Rican T3 plates, dropping the sample in a couple places. Maybe I need to try using two scalpels...or watch more videos by TCs.

3.3.2021 Plates looking better and growing faster each transfer. Plates cooling right now for the next transfer.
I've again failed to germ P. adiposa from a spore print taken from grocery store fruits. This plate looks to have two small areas that look close enough to mycelium to try a transfer; with this plate I just held a drying cap over the plate and tapped.

3.5.2021 One of the growths on the Pholiota adiposa germ plate looks much like the light, wispy myc I got from a Hericium erinaceus clone. So am taking a transfer and seeing what happens.

Edited by Narrator (03/06/21 01:19 AM)
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capntrips7
Moldsmith

Registered: 10/22/20
Posts: 91
Loc: Urithiru
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
#27109831 - 12/27/20 12:07 AM (2 months, 9 days ago) |
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Grow Log
Species: P. Cubensis Variant: B+, GT, APE, RW, AA+, AA, PM, SG, HO Species: P. Tampanensis
Abbreviations
Avery's Albino (AA), Albino Penis Envy (APE), Golden Teacher (GT), Huautla/Oaxaca (HO), Plantasia Mystery (PM), RustyWhyte (RW), Star Gazers (SG)
Plates
1-4. 2021-01-03 B+ on LME 5. 2021-01-11 T1 B+ from #1 on LME 6. 2021-01-11 T1 B+ from #3 on LME 7. 2021-01-14 GT on LME 8. 2021-01-14 T1 B+ from #1 on LME 9. 2021-01-17 T2 B+ from #5 on LME (1%) 10. 2021-01-17 T2 B+ from #6 on LME (1%) 11. 2021-01-17 T2 B+ from #5 on LME 12. 2021-01-17 T2 B+ from #6 on LME 13. 2021-01-19 T1 GT from #7 on BRF (1%) 14. 2021-01-19 T1 GT from #7 on BRF 15. 2021-01-19 T1 GT from #7 on LME (1%) 16-19. 2021-01-26 APE on LME (1% agar) 20-21. 2021-01-26 RW on LME (1% agar) 22-23. 2021-01-26 GT on LME (1% agar) 24. 2021-01-26 T2 B+ from #5 on LME 25. 2021-01-26 T2 B+ from #6 on LME 26. 2021-01-26 T2 B+ from #8 on LME 27. 2021-02-01 T3 B+ from #24 on LME 28. 2021-02-01 T3 B+ from #25 on LME 29. 2021-02-01 T3 B+ from #26 on LME 30. 2021-02-01 Test B+ from LC #1 on LME 31-32. 2021-02-03 AA+ on LME (1% agar) 33. 2021-02-03 T2 GT from #15 on LME 34. 2021-02-03 T1 APE from #16 on LME 35. 2021-02-03 T1 RW from #20 on LME 36. 2021-02-03 T1 GT from #22 on LME 37. 2021-02-23 AA on LME (1% agar) 38. 2021-02-23 AA+ on BRF (1%) 39. 2021-02-23 T3 GT from #33 on LME 40. 2021-02-23 T2 APE from #34 on LME 41. 2021-02-23 T2 RW from #35 on LME 42. 2021-02-23 T2 GT from #36 on LME 43. 2021-02-24 PM on LME (1% agar) 44. 2021-02-24 PM on BRF (1%) 45. 2021-02-24 SG on LME (1% agar) 46. 2021-02-24 HO on BRF (1%)
LC
1. 2021-01-26 B+ from plate #8 on LME
Jars
1-3. 2021-01-17 B+ a2g from #5 on oats 4-6. 2021-01-17 B+ a2g from #6 on oats 7-9. 2021-02-01 B+ from LC #1 on oats 10-15. 2021-02-03 B+ from #5 on oats
Tubs
1. 2021-02-03 B+ from jars #1-4,6 on coir, cased 2. 2021-02-25 B+ from jars #10-15 on coir, cased
Changelog
Day 1, 2021-01-03 Inoculated plates
Day 2, 2021-01-04 Prepped agar
Day 8, 2021-01-10 Germination among contams
Day 9, 2021-01-11 Made transfers
Day 12, 2021-01-14 Inoculated a plate and made transfers
Day 13, 2021-01-15 Prepped agar and T1 plates showing growth away from bacteria
Day 14, 2021-01-16 Prepped grain jars
Day 15, 2021-01-17 Prepped agar, made transfers, and inoculated grain jars
Day 17, 2021-01-19 Made transfers
Day 23, 2021-01-25 Prepped agar and LC
Day 24, 2021-01-26 Prepped spores, inoculated plates, and made transfers
Day 29, 2021-01-31 Prepped agar and grain jars
Day 30, 2021-02-01 Made transfers
Day 32, 2021-02-03 Prepped spores, inoculated a plate and grain jars, made transfers, cased a grain jar, and prepped coir and a tub
Day 52, 2021-02-23 Inoculated plates and made transfers
Day 53, 2021-02-24 Inoculated plates
Day 54, 2021-02-25 Inoculated plates and prepped coir and a tub
Details
Day 0
Made two batches of 200ml agar with 3.2g LME and 4g agar powder, the first with 2 drops neon green food coloring, and the second with 1g activated charcoal. PC for 20 minutes.
Day 1, 2021-01-03
Inoculated plates 1-4.
Day 2, 2021-01-04
Made two batches of 400ml agar with 6.4g LME, 8g agar powder, and 2 drops neon green food coloring. PC for 20 minutes.
Day 8, 2021-01-10
Germination amongst a wide array of contams:

Day 9, 2021-01-11
Made transfers to plates 5-6:


Day 12, 2021-01-14
Inoculated plate 7.
Made transfers to plate 8:

Day 13, 2021-01-15
Made two batches of 200ml agar, the first with 2g LME, 4g agar powder, and 2 drops of blue food coloring and the second with 2g BRF, 4g agar powder, and 2 drops of blue food coloring. PC for 20 minutes.
T1 plates showing growth away from bacteria:

Day 14, 2021-01-16
Boiled 275g oats in 1241ml water for 30 minutes. Discarded 627g GSW. Divided 549g hydrated oats and filled jars 1-6 with 85g each, discarding remainer. PC for 90 minutes.
Day 15, 2021-01-17
Made transfers to plates 9-12.
Inoculated jars 1-6.


Day 17, 2021-01-19
Made two batches of agar, the first 400ml with 6g LME, 8g agar, and 2g activated charcoal and the second 200ml with 2g LME, 4g agar, and 1g activated charcoal. PC for 20 minutes.
Made transfers to plates 13-15.

Day 23, 2021-01-25
Made three batches of agar, the first 400ml with 6.4g LME, 8g agar, and 4 drops of blue food coloring, the second 200ml with 3g LME, 2g agar, and 2 drops of green food coloring, and the third 200ml with 3.1g LME, 2g agar, and 1g activated charcoal. PC for 20 minutes.
Made four batches of LC, the first two 150ml with 0.6g LME, and the second two 50ml with 0.2g LME. PC for 20 minutes.
Day 24, 2021-01-26
Made three spore syringes, one each APE, RW, and GT.
Inoculated plates 16-23.
Made transfers to plates 24-26.

Day 29, 2021-01-31
Made 400ml agar with 6g LME, 6g, agar, and 3 drops of orange food coloring. PC for 20 minutes.
Boiled 250g oats in 1125ml water for 30 minutes. Discarded 430g GSW. Divided 492g hydrated oats and filled jars 37-42 with 75g each, discarding remainer. PC for 90 minutes.
Day 30, 2021-02-01
Made transfers to plates 27-29.

Tested LC 1 on plate 30.
Inoculated jars 7-9 with LC 1.
Day 32, 2021-02-03
Made AA+ spore syringe.
Inoculated plate 31.
Made transfers to plates 32-36.
Inoculated jars 10-15.
Cased leftover grain from jar 5.
Boiled water, poured over coir, mixed after 1 hour.
Prepped tub 1 with 396g spawn and 989g substrate, cased with 155g coir.


Day 52, 2021-02-23
Inoculated plates 37-38.
Made transfers to plates 39-42.
Day 53, 2021-02-24
Inoculated plates 43-44.
Day 54, 2021-02-25
Inoculated plates 45-46.
Boiled 813ml water, poured over 163g coir, and mixed immediately, reserving 60g.
Prepped tub 2 with 486g spawn and 829g substrate, cased with 60g coir.
Edited by capntrips7 (02/25/21 09:12 PM)
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Kromazone
10th form total concentration


Registered: 04/12/20
Posts: 208
Loc: Cl0uDn9n3
Last seen: 6 hours, 5 minutes
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: capntrips7]
#27109876 - 12/27/20 01:27 AM (2 months, 9 days ago) |
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LAGM 2021 
SPECIES: **P. CUBENSIS**
VARIENT: **RW** & **ABM**
DEC 29TH
*streaked plates with APE & ABM swabs

(said fuck it on the last and jammed the swab in the middle of the last cup)
01/04
All my plates we over loaded with bacteria so I managed to get a couple transfers of abm and got a plate to germinate with some rw spores I got.
Jan 16th Made a few transfers looking good so far

(Update) Jan 21st
Got some wbs I boiled, rinsed and pc for 2 hrs (I got an electric pc that only goes to 11 psi, so I pc for extra time) And knocked up a couple jars of wbs with the T1s . Also mixed some karo and water in a 100ml media bottle and through a couple transfers in it.
Jan 26th Here's a couple of my wbs jars now.

And hers the abm LC I made.

×××FEB 14×××
OK SO ITS BEEN A MINUTE, AND IVE GOT A COUPLE GOOD LOOKING JARS, THE RW IS ON ITS 1ST TRANSFER BECAUSE I HAD TO START OVER WITH IT. BUT THE ABM (ALBINO BLUE MAGNOLIA) I INOCULATED A COUPLE GRAIN JARS FROM THE LC I MADE IS ALMOST DONE COLONIZING.

And i had a decent agar culture from a swab i mislabeled but i think is malmak, and i put it to some wbs:
 Those should be done in a couple days.
LAST BUT NOT LEAST HERES MY NEW RW PLATE I TRANSFERED FROM A BACTERIAL PLATE:
 Ill take 1 or 2 transfers from that before i go to grain, update in a few days.
forgot to update.....
Skip ahead:
MARCH 3RD!!
I HAVE OFFICIALLY PINNED MY FIRST BULK SUBSTRATES! Pics incomming
Albino Blue Mags:

Malmak:

Edited by Kromazone (03/03/21 12:32 AM)
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lookintolearn
Stranger becoming Strangest



Registered: 06/02/20
Posts: 445
Loc: Up in the Cut
Last seen: 17 days, 4 hours
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Kromazone]
#27109900 - 12/27/20 02:10 AM (2 months, 9 days ago) |
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Oh boy I can't wait to see what all you mad lads put out for this!
I'm in!
Lookintolearn's Lookin to grow Mushrooms!
What I'll be growing:
P.Cubensis
-Starting my first PE since it's LAGM! -KSSS -Argentinian -{maybe} Texas Orange Cap if I find time!
My (late) start! 1/4/2021:
Took a PE syringe that i've been holding onto forever now and finally put it to some plates. I started with 8 plates and hoping to get a little something from at least one to continue my adventure. Will be starting other varieties here soon.

1/11/2021: Got some PE germination!

1/14/2021: Took a whole bunch of transfers from the PE germination, got alot more than I thought I would!

1/20/2021:
Have moved to my T2 transfers, all is looking well so far. Wispy growth concerning but is organized. Will be taking a few T2 and then T3 to grain to see how it goes!
 
1/28/2021: Have taken T3 on all my plates will be dropping to grain soon.
 
Edited by lookintolearn (01/29/21 01:18 PM)
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GringoCazadar
Stranger
Registered: 12/25/20
Posts: 1
Last seen: 1 month, 29 days
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Count me in! First post on shroomey, just started researching about a month ago and nows the best time to give growing a whirl!
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Tankie_J
I like having fun



Registered: 01/10/19
Posts: 444
Last seen: 7 hours, 6 minutes
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard] 2
#27109933 - 12/27/20 03:06 AM (2 months, 9 days ago) |
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Tankie’s LAGM 2021 Species: Pan Cyan Variant: Unknown -02JAN21- noc’d 3 lme plates via loop from MSS. One drop on two of the plates and the other got away from me a bit. All got a little zig-zag action.  -15JAN- MSS to brf puck -27JAN- Well here’s a breakdown of what I ended up doing:  Made this biopsy tool using a 5/16” leather punch and 1/16-309 stainless TIG wire and the wife’s hair tie (she had a brand new pack)  Suspected mold circled in red. Pan germ in blue.  Bottom side of germ  Screenshot of the transfer. I will admit it was stuck to the TIG wire a bit but came loose with a little turning action.  Then I split transfer into 4 sections with flame sterilized blade  What’s left in the puck All in all I’m happy with what went down. Took about 20 minutes to make the tool up. I will keep it in the myco tool box -3FEB-  Growth update Species: P. Cubensis Variants: Maz, Whytegold, WGxTOC, WGxBlueMeanie, APEU Mazatapec-02JAN21- 2 lme plates via loop from on of my own spore prints. One I tried a zig-zag from 12 o’clock down and the other just kinda swiped across the middle.  -11JAN- No germ on the maz yet -23JAN-  No germ on original maz plates. I just harvested two fruiting tubs and swabbed fresh spores. -29JAN-  Took a couple T1’s -3FEB-  Fresh spore T1’s update -05FEB-  The T1’s looked so good they went to grain. 1/2 plate per qt jar. -18FEB- Spawned 4 shoeboxes -27FEB- Pins baby  -05MAR- Started harvesting... they’re coming in at all different rates -06MAR-More harvesting  Whytegold-11JAN- I received an awesome package from ‘FK’ today and in it was a print labeled “Whytegold”. I haven’t read anything about this on the forum yet which has me pretty excited. It’s a rusty looking print (I didn’t get a pic of it). Used a sterile scalpel, scraped the print and jammed the spores into the agar. Not the best technique but I’m just not having any luck with the streak lately.  -16JAN- Germination on both plates. This plate was streak/sliced with a knife so I’m not 100% on all the growth. Looks healthy enough. Will let it grow some more before T1’s.  This plate was one single jab with a healthy amount of spores on the tip. No satellite germination has me happy. Will let grow out a little more before T1’s as well. -21JAN-  4-T1’s of Whytegold and 4 APEU -24JAN-  T1’s are taking off nicely and I have my eye on some strong areas for T2’s -3FEB-  Whytegold T2 update (4 plates on the right top and bottom) The two on the left are APEU T2’s. These will be going to grain tomorrow. -4FEB-  Whytegold transfer to 8 grain jars. First time using half a plate per jar... improvements to be made on speed. We’ll see how these go. Guess APEU is tagging along in this WG thread. Transferred to 4 grain jars. Dipped into my second batch of jars and noticed I over boiled a bit, a little clumpy on the shake.  -18FEB- Spawned 8 shoeboxes -27FEB- Fully colonized and about to pin -01MAR - 05MARPhoto dump uploaded in reverse chronological order -07MAR-Harvested 142g wet of random mature fruits from several tubs. Cloned one. Whytegold x Blue Meanie-25JAN-  Swabbed plates -29JAN-  Took three T1’s. If they’re clean they will go to grain from here to get the most genetic variability. I see other options at cross points but ran out of plates. -22FEB- Spawned 2 shoeboxes -06MAR-Knots and a few light/white pins could be something? Could just be Whytegold Whytegold x TOC-25JAN-  Swabbed plates -29JAN-  Took two T1’s. If they’re clean they will go to grain for most genetic variability. I see other options at cross points but ran out of plates. -22FEB- Spawned 1 shoebox -06MAR-Decent amount of orange pins. Some good clusters. Thinking I have TOC, no cross. Species: P. Baeocystis  GAME OVER -02JAN21- 2 lme plates via scraping the gills with a sterile blade onto the agar. I used the blade to push around some of the material on the agar. Donor is an urban harvested fruit out of the Seattle area. Pic shows dried fruit on top of the unopened maz print

-04JAN- Something has germinated. Both plates have an identical spot of something mysterious and it’s fucking fast.

-05JAN- The growth has shot spores... 

-06JAN- Tossed both plates, there’s no saving that mess. Not sure if I will try again. Hindsight: Should have taken prints after I found them.
Edited by Tankie_J (03/08/21 02:42 AM)
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D3monic
Weeb Trash



Registered: 05/06/18
Posts: 3,223
Loc: Year Zero
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Tankie_J] 1
#27109956 - 12/27/20 03:40 AM (2 months, 9 days ago) |
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D3's LAGM2021
 Hmmm let me thumb through the ole print catalog here... O piece of candy! Species: P Cubensis Var: x7x (f2), Melmac, Gandalf  1-2 streaked plates with x7x and Melmac 1-5 first germination on the x7x   Still waiting on melmac 1-9-21no germination on the MelMak streak plates but did get some on the dunk.. along with some bacteria off to the side and a satellite probably from when I cut the tip off the swab with dikes over the plate.  I transferred the dunk to a new plate and took xfers of the growth coming off it.  x7x ready for its first xfers as well though I’m a little leary on the growth. What you guys think? 1-18Took some T2’s today. 1-25Taking T3’s of the x7x f2 and the MelMak   1-31 Finally got around to streaking some plates with gandalf   x7x looking like it will be good to go to grain soon as it grows out a lil more  2-10 Took transfers and sent 2 plates of MelMak to grain. Possibly to use as masterd, will use a T4 plate for lc  Gave a buddy a Gandalf plate, pretty sure it was the only one to have germinated. 2-26-21x7x plate pin  Spore under magnification   Sending the f4 plates to grain today   Debating if I'll send these melmak or make LC or both... not quite happy with how they look yet.   Forgot about the Gandalf swab plate. Looks like I got something to work with even if it looks sketch
Edited by D3monic (02/26/21 08:43 PM)
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roarkell
The Roar Master!



Registered: 08/29/19
Posts: 1,698
Loc: Southern WI
Last seen: 1 hour, 5 minutes
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: capntrips7]
#27110030 - 12/27/20 05:44 AM (2 months, 9 days ago) |
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Quote:
capntrips7 said: I'm in.
Species: P. Cubensis Variant: B+, GT, AA+ Species: P. Tampanensis
The only spores I currently have are in a known bacterial B+ syringe, but this will be the excuse to finally try to make some antibacterial agar.
If all goes well, my first GT tub will be fruiting early in the year, so I can make a spore print. I also have grain colonizing with B+, AA+, and Tamp.
If you want some prints that are much cleaner let me know! Mail is still moving surprisingly fast...
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Puduwoke
mushroom enthusiastic

Registered: 06/25/17
Posts: 1,657
Last seen: 1 hour, 37 minutes
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: roarkell]
#27110416 - 12/27/20 11:49 AM (2 months, 8 days ago) |
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Panaeolus Tropicalis RDU / APE / Melmak: Puduwoke's Growlog 2021™ 
Info:
Date of / method of inoculation:
Grains used & colonizing time:
Time between harvest:
Species/strain:Panaeolus RDU / APE / Melmak
Culture: MS
Agar recipe: 10g agar, 7.5g Malt extract per 500 ml water. PCed for 40 min.
Substrate:
Environmental parameters temp/RH/FAE:
Casing:
Spawn to harvest:
Flush 1 weight:
Flush 2 weight:
Flush 3 weight:
Time between harvest:
———————————————————————————————— January 04, 2021 I swabbed a couple plates of each and broke of the Melmak and APE swab into the plate. First time doing this.
 ———————————————————————————————— January 09, 2021 First time getting APE and PE to germinate. Thanks to leaving the swab in the agar. It worked great. I'm so happy to have APE MS going oh also made T1 of the Pan RDU today. ———————————————————————————————— January 12, 2021 Make T1 from PE and APEs germ plates.
Edited by Puduwoke (01/12/21 02:41 PM)
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