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OfflineThe Dalcassian
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Sockadin]
    #27136927 - 01/09/21 07:06 AM (3 years, 19 days ago)

It's so weird writing the date month/day/year but today was T1 for the NST and Wild Coast. Hopefully the wild coast can go to grains next weekend 🤞


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InvisibleWall.E
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: The Dalcassian]
    #27136936 - 01/09/21 07:10 AM (3 years, 19 days ago)

What sock said, that's mold homie


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Offlineroarkell
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Wall.E]
    #27136986 - 01/09/21 07:47 AM (3 years, 19 days ago)

Got agar poured late last night... took about 75 transfer this morning... probably 40 or so were LAGM t1's... still no growth on my gyms tho... gonna swab some new spores to standard MEA hopefully today... we'll see what the day brings... lol


Edited by roarkell (01/09/21 07:48 AM)


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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: roarkell]
    #27137096 - 01/09/21 08:50 AM (3 years, 19 days ago)

Ambrose, I can't tell from your pics. Are you working with a SAB or a Flowhood?


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Offlineadhoc
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Wall.E]
    #27137166 - 01/09/21 09:19 AM (3 years, 19 days ago)

Quote:

Myc_Hunt said:
What sock said, that's mold homie




Appreciate the confirmation, both! 

If I want to keep trying with agar is there anything I can change up to try and get a better chance at coaxing out the myc?  I'm more interested in learning than the outcome, right now.  I know I can do cakes if I need to and get something to clone, but I'd like to understand the limits of agar more too.  (The syringe I'm using was purchased from a sponsor, for whatever that's worth.)


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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: adhoc]
    #27137207 - 01/09/21 09:34 AM (3 years, 19 days ago)

:mushdance::mushdance::mushdance::mushdance:January 9th, 2021:mushdance::mushdance::mushdance::mushdance:


Two steps forward, one step back.
The Z plate that had germinated now appears to be mold. However, the GT and PR plates continue to look good, despite being quite light and wispy (as good as they can on this PDA). On the upside, It looks like I have germination in both PFC plates. These were noc'd via MSS so kinda surprised they were not first to germ. Still very happy to see any favorable life in these guys right now.






Quote:

adhoc said:
Quote:

Myc_Hunt said:
What sock said, that's mold homie




Appreciate the confirmation, both! 

If I want to keep trying with agar is there anything I can change up to try and get a better chance at coaxing out the myc?  I'm more interested in learning than the outcome, right now.  I know I can do cakes if I need to and get something to clone, but I'd like to understand the limits of agar more too.  (The syringe I'm using was purchased from a sponsor, for whatever that's worth.)




I always assume things like these come down to my sterile tek. MSS will never be 100% so sometimes shit happens but at my level of experience I more readily blame myself. Sorta of a Occam's Razor moment. Me fucking up my movements and technique requires less change from the status quo than most other possibilities.

EDIT: That is not to say we are at the same experience level. Just some personal insight and :2cents:


Edited by GypsyBastard (01/09/21 09:38 AM)


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OfflineOne of Us
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard] * 1
    #27137240 - 01/09/21 09:47 AM (3 years, 19 days ago)

January 9 2021:

Ok, so the pan plates blew tf up. Should've checked on them earlier. I don't have much experience with pans, so my plan is to take 15-20 transfers from these plates, and take each of those to at least t3 before growing each culture in a small tray. I'll use a Jcm style FC



I will also be taking transfers from the Pearly gates  today:



I've got germination on the subcubensis and ps. Cyanescens at the moment as well, but I'll let these grow out a bit more.



No sign of life from the caerulipes plates as of yet.....


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Offlineadhoc
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
    #27137258 - 01/09/21 09:52 AM (3 years, 19 days ago)

Quote:

GypsyBastard said:
I always assume things like these come down to my sterile tek. MSS will never be 100% so sometimes shit happens but at my level of experience I more readily blame myself. Sorta of a Occam's Razor moment. Me fucking up my movements and technique requires less change from the status quo than most other possibilities.

EDIT: That is not to say we are at the same experience level. Just some personal insight and :2cents:




No worries, I know what you're saying.  I'm encouraged by the fact that growth only looks to be happening in the spots that I streaked, but you are right that I'm not to the point where technique couldn't also be a contributing factor.


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OfflineTankie_J
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: One of Us]
    #27137435 - 01/09/21 11:02 AM (3 years, 19 days ago)

Hell yeah One Of Us! All those look great!


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OfflinePitcherCrab
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Tankie_J]
    #27137500 - 01/09/21 11:36 AM (3 years, 19 days ago)

Forgive me ignorance, what does ESS stand for? And if I'm not mistaken it's PE x KSSS right?


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PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
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“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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OfflineSockadin
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: adhoc] * 1
    #27137556 - 01/09/21 12:13 PM (3 years, 19 days ago)

I would start with a new plate and a new shot of spores. There are so many different vectors it could be the syringe or it could be that you need to tighten something up. I do not think there is anything in those plates worth saving. Molds are so much harder than transferring away from Satellite bacteria colonies.


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OfflineBrownBear
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: PitcherCrab]
    #27137619 - 01/09/21 12:48 PM (3 years, 19 days ago)

Quote:

PitcherCrab said:
Forgive me ignorance, what does ESS stand for? And if I'm not mistaken it's PE x KSSS right?




Yes. Envy ss or ess is a cross between pe and ksss. I believe muda is the one who created and stabalized it, if I am not mistaken.


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OfflinePitcherCrab
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: BrownBear]
    #27137638 - 01/09/21 12:59 PM (3 years, 19 days ago)

Cool, thanks for clarifying!


--------------------
PC's LAGM 2021 (TOC & TWC)
P. natalensis Growlog 2021
Pans for PC Fall 2021 Growlog

“I must not fear. Fear is the mind-killer. Fear is the little death that brings total obliteration. I will face my fear. I will permit it to pass over me and through me. And when it has gone past I will turn the inner eye to see its path. Where the fear has gone there will be nothing. Only I will remain.” - Bene Gesserit Litany Against Fear



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Invisiblebigfootscreepyuncl
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: BrownBear]
    #27137639 - 01/09/21 01:00 PM (3 years, 19 days ago)

gypsy - would you mind adding P. cubensis Nepali Chitwan to my LAGM list? It's a sad day on the farm today as I'm coming to the realization that my first attempt at growing has gone tits up due to a bacterial infection and will be getting dumped  :icecreamsadness:

I'm not one to give up so I'm going to get some more spores to agar today and see if I can't improve on some of my mistakes. Fortunately it shouldn't be too hard because there were sooooooo many lol


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InvisibleD3_Myc
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: bigfootscreepyuncl]
    #27138003 - 01/09/21 04:16 PM (3 years, 19 days ago)

1-9-21


no germination on the MelMak streak plates but did get some on the dunk.. along with some bacteria off to the side and a satellite probably from when I cut the tip off the swab with dikes over the plate.



I transferred the dunk to a new plate and took xfers of the growth coming off it.



x7x ready for its first xfers as well though I’m a little leary on the growth. What you guys think?



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Offlineredbee
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: redbee]
    #27138009 - 01/09/21 04:21 PM (3 years, 19 days ago)

I've got germination! Update below.

iambrownbear, I'll race you to harvest with those RWs :stayfunky:

One of Us, looking great, I'm curious to follow a pan grow log, I haven't yet looked into those

roarkell, goddamn you are a busy dude, that rules!


Quote:

redbee said:

JANUARY 09, 2021

Germination = achieved! :sunny:



tl;dr looks like I'll have some viable transfer options for both the APEU and RW, and the quickest germination was definitely from the plates where I either dropped in the cotton swab tip or successfully scraped cotton strands onto the agar. I'm seeing contams on one plate from each variety, but, y'know, gills are dirty.

Here are the detailed notes... in the photos, it's APEU on the left and RW on the right.

APEU:
Swab Plate A: streaked with swab, looks like myc on the right and contam on the left
Swab Plate B: streaked with swab, no visible growth yet
Swab Plate C: shredded cotton strands, myc showed up quickly (lumpy b/c the agar is kinda chopped up)
Swab Plate D: shredded cotton strands, myc also looks good so far

Rusty Whyte:
Swab Plate A: shredded cotton strands, holy mother of moldy god this one looks gross IRL. (This was the plate that received the most abuse due to dull blade/stuck-cotton, and I would have been astounded had it not contaminated)
Swab Plate B: streaked with swab, lookin' like wispy RW myc
Swab Plate C: cut the swab tip and dropped it onto the plate, currently all myc'd up, fuzzy and glorious







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:sporedrop:  redbee's LAGM 2021  :sporedrop:


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Offlineroarkell
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: redbee]
    #27138056 - 01/09/21 04:51 PM (3 years, 19 days ago)

Quote:

D3monic said:

x7x ready for its first xfers as well though I’m a little leary on the growth. What you guys think?






The bigger colony off to the right looks viable... 4-7 o'clock area would be my starting spot... the are from 9 to noon could be okay too... also for me lately, ill grab a piece of all those smaller satellite colonies over to the left... but thats just me... lol

I still haven't wrapped my head around all the back and forth some mycelium will do in terms of growth patterns... this x7x and my golden halos both go fuxking bonkers... lol

@redbee- if I slow down ill die! Lol... gonna make another batch of agar tonight since I blew thru those last 75 today... lol. Got some goofy APEs to clone... and swab... *wink wink*


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InvisibleD3_Myc
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: roarkell]
    #27138062 - 01/09/21 04:54 PM (3 years, 19 days ago)

I took 12 xfers all together between two swab plates. A little representative sample from the more promising little growths and a few the larger ones.


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Offlineroarkell
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: D3_Myc]
    #27138078 - 01/09/21 05:02 PM (3 years, 19 days ago)

Nice... I did a shit ton of t1s this morning... especially on Quirkmeister92's melmak that I'm running for LAGM... I think I took a piece of every single colony on all the swab plates... lol ive had a hell of a time with melmak and PE... so im really crossing my fingers that something nice will come outta one of those motherfuckers... lol


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Offlineredbee
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: roarkell]
    #27138090 - 01/09/21 05:09 PM (3 years, 19 days ago)

Quote:

roarkell said:

if I slow down ill die! Lol...




That's what I keep telling my therapist!  :lol:  ...but I guess I'm paying her to NOT believe me...


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:sporedrop:  redbee's LAGM 2021  :sporedrop:


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