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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 1 hour, 42 minutes
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: roarkell] 1
#27131517 - 01/06/21 07:43 PM (3 years, 22 days ago) |
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Finally got germ plates poured so I can start streaking in the morning.

Only potential problem was I turned the hood on low to let it run while the bottle cooled and forgot to turn it on high when I poured.
I like these cell treat ones so far. It’s super nice having 20 to a sleeve so I don’t have to rewrap and store five extra plates each time.
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LAGM2020     
Edited by A.k.a (01/06/21 07:47 PM)
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Jarhead3521
Grindin’



Registered: 09/26/19
Posts: 514
Loc: Amongst the pines
Last seen: 5 months, 22 days
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: A.k.a]
#27131549 - 01/06/21 07:53 PM (3 years, 22 days ago) |
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Huatla germ plates were not myc, tossing and try again. I did swab some PEU last night, maybe some food comes of that.
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Doctor Mario
🍄🍄🍄🍄🍄



Registered: 08/07/20
Posts: 1,894
Loc: 🇺🇸
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Jarhead3521]
#27131554 - 01/06/21 07:56 PM (3 years, 22 days ago) |
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Is running the fan on high nessecary or precautionary?
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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: A.k.a]
#27131563 - 01/06/21 08:01 PM (3 years, 22 days ago) |
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Quote:
A.k.a said: Finally got germ plates poured so I can start streaking in the morning.

Only potential problem was I turned the hood on low to let it run while the bottle cooled and forgot to turn it on high when I poured.
I like these cell treat ones so far. It’s super nice having 20 to a sleeve so I don’t have to rewrap and store five extra plates each time.
I'm going your route aka and grabbing those 100mm plates at bulk. But I'm going to get the sectored plates so I can easily drop colonized sectors into jars
 I have like 700-ish 90mm plates but like, I've been wanting to try sectored plates.
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Meditation Principles Silence: Giving you room to listen / Stillness: Giving you room to feel / Spaciousness: Just giving you room
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A.k.a
Stranger



Registered: 10/27/19
Posts: 16,782
Loc: Gaming the system
Last seen: 1 hour, 42 minutes
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Doctor Mario]
#27131581 - 01/06/21 08:06 PM (3 years, 22 days ago) |
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Quote:
Doctor Mario said: Is running the fan on high nessecary or precautionary?
I’m not sure, but I’ll be finding out over the next week
I was told to use the high setting but I’m pretty confident the plates will be ok. I was fast and didn’t open them more than needed.
Right on man I thought about using those Y plates too for the same reason until I got going on lc.
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LAGM2020     
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MycoBrainz
Mycotic



Registered: 03/14/15
Posts: 615
Loc: West Coast
Last seen: 4 months, 10 days
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: A.k.a]
#27131640 - 01/06/21 08:27 PM (3 years, 22 days ago) |
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Just did first transfers of the Z strain,PE, and APE. Then second transfers of all the Pan cyans (Hawaiian, Aussie, and Alabama), P.Bisporus, RustyWhites, and AA+. Getting another batch of agar ready for another 100 plates. I'm Rollin SAB on kitchen table with Ozone generator running 24/7. Only had 4 plates show Bacteria that wasn't from a swab.
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PFC x Creeper Lets Get Stoned
Edited by MycoBrainz (01/06/21 08:29 PM)
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RoscoeReturns
Crotchety chode man



Registered: 02/12/18
Posts: 1,738
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: roarkell]
#27131785 - 01/06/21 09:39 PM (3 years, 21 days ago) |
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Quote:
roarkell said:
Quote:
06DEC2021: The swab dunk on the SAT threw me off, thought I had some growth but its just mold...  Nothing on the gyms either... Pic dump of the rest of the plates im taking transfers from.
 The seventh pic is the only PDA plate to harbor life... but that Fiji was a few days ahead of the rest so that may have something to do with it...
Updated OP... gonna get these transfers knocked out and then I need some sleep...
I think you need some sleep. 06DEC2021? Getting a head start on LAGM 2022?
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bigfootscreepyuncl
Stranger


Registered: 11/15/20
Posts: 3,717
Loc: Gamehenge
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Quote:
1/7/21 We have germination! After nearly a week of checking on my plates several times a day, posting on this thread and undoubtedly pissing off some of the people following along with my complaining I finally noticed some baby mycelium on one of my APE plates! Out of my GT, PE and APE I wasn't expecting to get anything out of this one in particular.

Sorry for the clarity of the pictures. I poured some crystal clear plates, got impatient so I moved my stack closer to my heat vent and that condensed them like a bastard...live and learn. The closeup was attempted to be taken through my jewelers loop. Cheers, mates!
I finally spotted some baby mycelium on one of my APE plates tonight (MS syringe to agar)! I do have to apologize for my complaining lately lol. I'm unemployed 3-4 months every year and I'm in the middle of that stretch right now with nothing else to occupy my mind and have become obsessed with this project. Anyway, my OP is updated and my LAGM21 is underway. Thanks for the support. Cheers!
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I 5318008 NOT a virgin!
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GypsyBastard
Misanthrope


Registered: 11/30/20
Posts: 846
Loc: The Mighty Boosh
Last seen: 2 years, 10 months
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Mini update, 1 Z plate is showing myc growth now. The rest shouldn't be far behind. I used PDA because move I lost my LME and I am remembering why I stopped using PDA. Just never seemed to get the same full aggressive myco I did with LME.
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TigerStrike
carpophore locator



Registered: 12/22/20
Posts: 315
Last seen: 1 year, 9 months
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: A.k.a]
#27132070 - 01/07/21 01:06 AM (3 years, 21 days ago) |
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Quote:
A.k.a said: Finally got germ plates poured so I can start streaking in the morning.

Only potential problem was I turned the hood on low to let it run while the bottle cooled and forgot to turn it on high when I poured.
I like these cell treat ones so far. It’s super nice having 20 to a sleeve so I don’t have to rewrap and store five extra plates each time.
There is blood magic and alchemy involved, TC's are immune to slow hood speed contams.
I'm going your route aka and grabbing those 100mm plates at bulk. But I'm going to get the sectored plates so I can easily drop colonized sectors into jars
 I have like 700-ish 90mm plates but like, I've been wanting to try sectored plates.
before you do that just hear me out. Consider picking up 10x 60mm plates they fit inside a wide mouth quart all 10 easy sterilizing, no wasted plastic.
I also keep on hand I have 4 cases. i have a box of 150mm plates 800 or so slim line 500 regular slideable and another 500 something else. all from weber i started really loving and using my glass ones recently though.
Edited by TigerStrike (01/07/21 01:13 AM)
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TigerStrike
carpophore locator



Registered: 12/22/20
Posts: 315
Last seen: 1 year, 9 months
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: mushhead]
#27132079 - 01/07/21 01:11 AM (3 years, 21 days ago) |
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Duplicate post
Edited by TigerStrike (01/07/21 01:12 AM)
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Ballzagna
Carb Daddy



Registered: 10/09/19
Posts: 619
Last seen: 13 days, 12 hours
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GypsyBastard]
#27132090 - 01/07/21 01:21 AM (3 years, 21 days ago) |
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Gypsy you ever try grain water? it's pretty abundant in this hobby lol and it's very effective. My bag of LME is collecting dust and slowly hardening into a solid block because I don't use it anymore.
-------------------- No one likes a naysayer It's probably fine. Even if it's not fine, it'll eventually be fine. So it's fine. PE Adventure
Photosynthesis: A Morning Ritual Ballzagna's Tek Compendium and Notes for Noobs 
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lookintolearn
Stranger becoming Strangest



Registered: 06/02/20
Posts: 574
Loc: Up in the Cut
Last seen: 1 month, 18 days
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Ballzagna] 1
#27132113 - 01/07/21 02:00 AM (3 years, 21 days ago) |
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Now I can finally start LAGM the right way 
Hoping I can get some germination from my PE plates, I really wanna get started on transfers already
-------------------- Don't be afraid of feeling the feelings Lookin to LAGM 2021    Looking to start growing? Read through Bod's Introduction to Everything Looking to start agar? Start with Alien's Holy Grail Looking to perfect your transfers? Start with D3monic's Perfect Transfers Looking for easiest prep to Coir ever? Eat's UNBUCKET Tek Looking to start LC? Try LI first! Munch's super easy Blenderless LI
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mushhead
Potato Devourer



Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: TigerStrike]
#27132137 - 01/07/21 02:54 AM (3 years, 21 days ago) |
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Quote:
TigerStrike said:
Quote:
A.k.a said: Finally got germ plates poured so I can start streaking in the morning.

Only potential problem was I turned the hood on low to let it run while the bottle cooled and forgot to turn it on high when I poured.
I like these cell treat ones so far. It’s super nice having 20 to a sleeve so I don’t have to rewrap and store five extra plates each time.
There is blood magic and alchemy involved, TC's are immune to slow hood speed contams.
I'm going your route aka and grabbing those 100mm plates at bulk. But I'm going to get the sectored plates so I can easily drop colonized sectors into jars
 I have like 700-ish 90mm plates but like, I've been wanting to try sectored plates.
before you do that just hear me out. Consider picking up 10x 60mm plates they fit inside a wide mouth quart all 10 easy sterilizing, no wasted plastic.
I also keep on hand I have 4 cases. i have a box of 150mm plates 800 or so slim line 500 regular slideable and another 500 something else. all from weber i started really loving and using my glass ones recently though.
I am branching out slowly. Thanks for the suggestions pal!
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roarkell
The Roar Master!



Registered: 08/29/19
Posts: 2,223
Loc: Southern WI
Last seen: 6 months, 21 days
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: RoscoeReturns] 1
#27132164 - 01/07/21 03:59 AM (3 years, 21 days ago) |
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Quote:
RoscoeReturns said:
Quote:
roarkell said:
Quote:
06DEC2021: The swab dunk on the SAT threw me off, thought I had some growth but its just mold...  Nothing on the gyms either... Pic dump of the rest of the plates im taking transfers from.
 The seventh pic is the only PDA plate to harbor life... but that Fiji was a few days ahead of the rest so that may have something to do with it...
Updated OP... gonna get these transfers knocked out and then I need some sleep...
I think you need some sleep. 06DEC2021? Getting a head start on LAGM 2022?
Fuck... now I'm wondering if I labeled all my transfers wrong... lmao... and I still didn't get to sleep until like 3 hours later...
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 12 days, 23 hours
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: TigerStrike]
#27132201 - 01/07/21 05:10 AM (3 years, 21 days ago) |
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Quote:
TigerStrike said: ...Consider picking up 10x 60mm plates they fit inside a wide mouth quart all 10 easy sterilizing... ...using my glass ones recently though.
hEY TigerStrike, can you describe sterilizing those plates in jars. Also I wonder how you get them out. I assume you pour later? Thanks
What I do for my 100mm plates: Line up the markers on the bottom plates Wrap in foil, 5 plate stacks Put on oven rack Turn oven on 450 F, 2 hrs set phone alarm. Let cool in oven - 1:30 hrs Store in 2 Shoeboxes
P.S. See, see, that's what I'm talkin about! Real life struggles with myc and life! This what LAGM brings, contam, getting ripped off by cheap ass plates, having to move, or worse, work! Dropping plates! Impatience! It's all here, way different than what you see usually on the Shroomery. Even TCs get hit with contam or life! AND this is the reason for starting at the same time, look at all the different reports of agar coming in! Next comes the transfers, then it kinda spreads out and drifts off. 
Edited by Inthepit (01/07/21 05:26 AM)
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coversall
إِنْ شَاءَ ٱللَهُ



Registered: 06/06/20
Posts: 2,749
Loc: संसार
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Inthepit]
#27132212 - 01/07/21 05:29 AM (3 years, 21 days ago) |
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Quote:
Inthepit said:
Quote:
TigerStrike said: ...Consider picking up 10x 60mm plates they fit inside a wide mouth quart all 10 easy sterilizing... ...using my glass ones recently though.
hEY TigerStrike, can you describe sterilizing those plates in jars. Also I wonder how you get them out. I assume you pour later? Thanks
What I do for my 100mm plates: Line up the markers on the bottom plates Wrap in foil, 5 plate stacks Put on oven rack Turn oven on 450 F, 2 hrs set phone alarm. Let cool in oven - 1:30 hrs Store in 2 Shoeboxes
P.S. See, see, that's what I'm talkin about! Real life struggles with myc and life! This what LAGM brings, contam, getting ripped off by cheap ass plates, having to move, or worse, work! Dropping plates! Impatience! It's all here, way different than what you see usually on the Shroomery. Even TCs get hit with contam or life! AND this is the reason for starting at the same time, look at all the different reports of agar coming in! Next comes the transfers, then it kinda spreads out and drifts off. 
Oven baking glass plates in foil is so easy. Dry heat. No stress about moisture. Sorted.
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TigerStrike
carpophore locator



Registered: 12/22/20
Posts: 315
Last seen: 1 year, 9 months
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: coversall]
#27132218 - 01/07/21 05:37 AM (3 years, 21 days ago) |
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Load upside down into wide mouth jar. Cover with aluminum foil when there are 10 2 or 3 stick above jar opening. Cover with foil. Uncover in flowhood or sab then dump them on 1 spot and lift jar away life it was a sleeve bag.
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Inthepit
Aum Mani Padme Hum



Registered: 08/20/19
Posts: 1,742
Loc: Puerto Rico
Last seen: 12 days, 23 hours
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: TigerStrike]
#27132225 - 01/07/21 05:43 AM (3 years, 21 days ago) |
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Quote:
TigerStrike said: Load upside down into wide mouth jar. Cover with aluminum foil when there are 10 2 or 3 stick above jar opening. Cover with foil. Uncover in flowhood or sab then dump them on 1 spot and lift jar away life it was a sleeve bag.
Is that in the PC or oven?
Ooooooooooh, so sexy! I like the lift the jar part. I'm really not comfortable unwrapping the foil off the plates. Last time I did it outside the SAB. So far I've got 1% auto-contam, well that's down from 20% at least. Still trying to visualize if I'd like 60mm...
Oh, ya, and yes I must be a former Marine not ex. Pole climber, AKA, like telephone company. Imagine being up a 30' pole getting shot at, ha, ha,...
Edited by Inthepit (01/07/21 05:53 AM)
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TigerStrike
carpophore locator



Registered: 12/22/20
Posts: 315
Last seen: 1 year, 9 months
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Inthepit]
#27132237 - 01/07/21 05:52 AM (3 years, 21 days ago) |
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see this vid. dudes crazy and hilarious
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