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Invisiblemushhead
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I'm a teapot


Registered: 08/22/14
Posts: 2,215
Loc: Dimension X-124
Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: A.k.a]
    #27126796 - 01/04/21 06:09 PM (3 years, 24 days ago)

I try to go for 90mm dishes if possible in bulk.
A.k.a, why use the larger plates?


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OfflineA.k.aM
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: mushhead]
    #27126833 - 01/04/21 06:26 PM (3 years, 24 days ago)

Honestly I just bought the cheapest case I could find this time. I probably should’ve gotten smaller ones since I’m mostly using lc these days and really just want to slant some stuff so I don’t have to start with spores.

Although with the big ones you can get to t3 on one plate if you want to. That’s kinda what I’ve been doing and then soon as I get growth off the third wedge make lc from it.


--------------------
LAGM2020


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OfflineEugene Gesuale
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: A.k.a]
    #27126852 - 01/04/21 06:37 PM (3 years, 24 days ago)

Good to know


--------------------
Everything in life is a trade-off.

All posts made by this account are purely satirical in nature.


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OfflineMr. Mushie

Registered: 01/16/20
Posts: 667
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Eugene Gesuale]
    #27126891 - 01/04/21 07:05 PM (3 years, 24 days ago)

Out of stock currently but....

https://www.kartersci.com/Petri_Dishes_PS_Material_variety_of_dimensions_p/pc-236s.htm

Cheapest price i've found. Shipping is ridiculous but still cheaper than any other 500ct case with shipping.

If you get some media bottles (great price for Pyrex or Karter Sci brand) and some centrifuge tubes, the shipping is negligible.


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InvisiblemushboyMDiscord
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Mr. Mushie]
    #27126910 - 01/04/21 07:14 PM (3 years, 24 days ago)


i found a print from cbk from before the first lagm. aa+


:awesomenod:

sorry im late!


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OfflineNichrome
I'm a torso!
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Registered: 12/17/18
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: mushboy]
    #27127030 - 01/04/21 08:14 PM (3 years, 23 days ago)

Way cool! What year is that print from? AA+ has been done so many times now (for good reason) it will be cool to see what those spores do.

:popcorn:


--------------------
Better to be deprived of food for three days, than tea for one.


Freedom is not the right to do as you please, but the liberty to do as you should. ~Emerson


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OfflineTankie_J
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Registered: 01/09/19
Posts: 537
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Tankie_J]
    #27127057 - 01/04/21 08:32 PM (3 years, 23 days ago)

Update on Psilocybe Baeocystis:

Quote:

Tankie_J said:
Species: P. Baeocystis


-02JAN21-
2 lme plates via scraping the gills with a sterile blade onto the agar. I used the blade to push around some of the material on the agar. Donor is an urban harvested fruit out of the Seattle area. Pic shows dried fruit on top of the unopened maz print



-04JAN-
Something has germinated. Both plates have an identical spot of something mysterious and it’s fucking fast.





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InvisibleDoctor Mario
🍄🍄🍄🍄🍄
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Registered: 08/07/20
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Mr. Mushie]
    #27127120 - 01/04/21 09:19 PM (3 years, 23 days ago)

Quote:

Mr. Mushie said:
Out of stock currently but....

https://www.kartersci.com/Petri_Dishes_PS_Material_variety_of_dimensions_p/pc-236s.htm





Those are vented. Do you cover the vents with micropore or somethin?


--------------------



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OfflineGrinchGrower
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Doctor Mario]
    #27127136 - 01/04/21 09:31 PM (3 years, 23 days ago)

I always buy vented  :shrug:
I think it's ideal if you cover them with parafilm for some GE to the culture
edit: found what led me to believe this...
Quote:


Do I want vented or non-vented petri dishes?

RogerRabbit said:
Vented.  The mycelium needs the gas exchange, and your parafilm will help to prevent drying.  If you use non-vented, you still need to wrap with parafilm, but then gas exchange is reduced to the point that growth stalls.  I had to donate 3/4 case of non-vented dishes to a local high school because they just wouldn't work properly.  Growth would stall after a few days, and bacteria is encouraged.
RR




Edited by GrinchGrower (01/04/21 10:13 PM)


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OfflineTigerStrike
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Doctor Mario]
    #27127143 - 01/04/21 09:36 PM (3 years, 23 days ago)

Damn it guys I might have a problem. No Germination yet and this shitake is on the same ship as bitcoin.



I am down to 2 plates not overtaken and a possibility to get this Agaricus B. out of 5.

May have to restart.

Worst case, If that shitake one is my last one ill transfer the shitake away tomorrow if not too late.


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OfflineFailboat
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: TigerStrike]
    #27127228 - 01/04/21 10:39 PM (3 years, 23 days ago)

I've been getting everything started and I seem to have a mystery print I got from UK in September 2019. There's no label nor a sender marked. It was however taped inside a blank bristol v brom betting sheet. The print was thick as soot and printed on a small scrap of foil placed inside more folded foil. The baggie was with the seal cut off and taped over to attach to the betting card.

Otherwise I am also growing TWC courtesy of Ted.


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OfflineMr. Mushie

Registered: 01/16/20
Posts: 667
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GrinchGrower]
    #27127238 - 01/04/21 10:47 PM (3 years, 23 days ago)

Quote:

Doctor Mario said:
Quote:

Mr. Mushie said:
Out of stock currently but....

https://www.kartersci.com/Petri_Dishes_PS_Material_variety_of_dimensions_p/pc-236s.htm





Those are vented. Do you cover the vents with micropore or somethin?



Quote:

GrinchGrower said:
I always buy vented  :shrug:
I think it's ideal if you cover them with parafilm for some GE to the culture
edit: found what led me to believe this...
Quote:


Do I want vented or non-vented petri dishes?

RogerRabbit said:
Vented.  The mycelium needs the gas exchange, and your parafilm will help to prevent drying.  If you use non-vented, you still need to wrap with parafilm, but then gas exchange is reduced to the point that growth stalls.  I had to donate 3/4 case of non-vented dishes to a local high school because they just wouldn't work properly.  Growth would stall after a few days, and bacteria is encouraged.
RR








:whathesaid:

I think everyone uses vented plates, I don't know why you wouldn't. They work as expected with Glad Cling Wrap or Parafilm.


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OfflineNarrator
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Registered: 11/30/20
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GrinchGrower]
    #27127290 - 01/05/21 12:03 AM (3 years, 23 days ago)

RR seems use "vented" to refer to a petri dish with a looser fitting top, the link to "vented" dishes seem to have holes in the top. Yes? No?


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OfflinePuduwoke
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Registered: 06/25/17
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Narrator]
    #27127301 - 01/05/21 12:28 AM (3 years, 23 days ago)

Havent updated my log yet but I'm finally in the race my mushroom family. Love all you guys. This community means a lot to me :hug:




First time put a whole swab on a plate. Have been having trouble getting pe and ape to germinate. Hopefully this helps :rockon:



--------------------
:sporedrop:Trade List:sporedrop:

LAGM2021


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OfflinecronicrFacebook
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Narrator]
    #27127305 - 01/05/21 12:32 AM (3 years, 23 days ago)

Quote:

Narrator said:
RR seems use "vented" to refer to a petri dish with a looser fitting top, the link to "vented" dishes seem to have holes in the top. Yes? No?



yes by vented he simply means the gap between the bottom and lid


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


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Invisiblecoversall
إِنْ شَاءَ ٱللَهُ
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Registered: 06/06/20
Posts: 2,749
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Puduwoke]
    #27127307 - 01/05/21 12:33 AM (3 years, 23 days ago)

Quote:

Puduwoke said:
Havent updated my log yet but I'm finally in the race my mushroom family. Love all you guys. This community means a lot to me :hug:




First time put a whole swab on a plate. Have been having trouble getting pe and ape to germinate. Hopefully this helps :rockon:






Oh man I can't wait to see the RDU, I think they look fantastic!

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~

05.01.21


Yeah yeah yeah, some signs of germination the pan cyan BVI plates. But nothing on the galindoi yet. If they don't germ then I'll start up a different mushroom. Will post some boring pics of specs on agar after work.

~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~


--------------------

..:: E V E R Y  ::..

..:: New? Start here. ::..
..:: How I Panaeolus. From Agar to Tea ::..


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OfflineGrinchGrower
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: Narrator]
    #27127325 - 01/05/21 12:52 AM (3 years, 23 days ago)

Quote:

Narrator said:
RR seems use "vented" to refer to a petri dish with a looser fitting top, the link to "vented" dishes seem to have holes in the top. Yes? No?




Yes and no. I'm pretty sure that the "vented" style you think has holes above are just small plastic bumps on the lid to raise it very slightly.


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Offlineadhoc
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: GrinchGrower] * 1
    #27127598 - 01/05/21 06:17 AM (3 years, 23 days ago)

My "Let's All Grow Copious Amounts of Bacteria 2021" grow is off to a flying start. 




Made up 2 more plates this morning, hopefully I caught the one mushroom spore in the syringe this time.


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OfflineHaywire
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: adhoc]
    #27127673 - 01/05/21 07:40 AM (3 years, 23 days ago)

I must admit I started a bit earlier than january first due to being on holidays.
this is TOC with already a first transfer done.
Also started LC with wedge from germ plate.



--------------------
Ciao mamma, guarda come mi diverto


My grows :mushroom2: Outdoor patches


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OfflineGypsyBastard
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Re: LAGM2021- Official Let's All Grow Mushrooms 2021 [Re: mushboy]
    #27127862 - 01/05/21 10:02 AM (3 years, 23 days ago)

Ok, let's see if I can touch base with everyone here.
First off, I love seeing 24 new posts on this thread when I log in!

Quote:

roarkell said:
Quote:

A.k.a said:
1/4/2021






I just finished a case of those and I wasn't super impressed... I like the Thomas Scientific, personally.




I have only ever used the celltreat as well. And like A.k.a, it is the only case I have ordered and still have about 80 left. I really had no complaints but that may be because I have no frame of reference. Also like A.k.a, I ordered the 100mm simply because they were the cheapest at the time I could find. I was not thrilled with the size at first. Just seemed like a lot of wasted space and agar, but once I figured out you can use the same plate for multiple transfer I did warm back up to them a bit. Still, kinda want to try some smaller ones on my next order. Just not sure I want to commit to a whole case before I find out if I like them.

Quote:

Tattersail said:
Last night I finally got spores swiped (well, scalpeled) to agar :smile:  For spores I like using these pint-size PP5s as no-pour agar plates, they're nice and roomy. They take up a lot of space so I did only one for each print. KSSS and a Gymnophilus. The KSSS is labelled as an in-vitro print so it might actually be very clean, however there is a good chance my poor sterile technique may have negated that. We shall see!



I'm thinking I might buy some proper petris, and pour agar for this grow. The visibility of these tubs is poor, there seems no point in taking photos.



That is a big part of my dislike for no-pours. No matter what container I used or procedure, etc., I never could get them as clear and condensation free as the real thing. That and the space saving side of it. Petri dishes IME stack more easily and reliably. I go so frustrated with my stacks of no pours tumbling over at the slightest bump of the table and a stack of 3 or 4 no pours took up as much room as 8 or 10 plates.

Quote:

Mr. Mushie said:
Out of stock currently but....

https://www.kartersci.com/Petri_Dishes_PS_Material_variety_of_dimensions_p/pc-236s.htm

Cheapest price i've found. Shipping is ridiculous but still cheaper than any other 500ct case with shipping.

If you get some media bottles (great price for Pyrex or Karter Sci brand) and some centrifuge tubes, the shipping is negligible.




Yeah those are definitely the cheapest I have seen. But yes, you are also not lying about the shipping either. Before shipping they are like half price. After shipping it's like a $15 savings. haha. but hey, 15 bucks is 15 bucks and there have been times in life that $15 would have significantly changed my circumstances. Hopefully they re-up before I kill my current case. Thanks for the share. :smile:

Quote:

mushboy said:

i found a print from cbk from before the first lagm. aa+


:awesomenod:

sorry im late!




mushboy that is killer! I can think of no better time to germ that print than now. :smile: I'll get you added in the roster shortly. Thanks for joining us!


:mushdance::mushdance::mushdance::mushdance::mushdance::mushdance::mushdance::mushdance::mushdance:

Ok, quick update: A Haiku


No germination
Except some bacteria
Guess I'll wait longer


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