Clc420 said:

D3monic said:

It’s been found that the magenta and purple can mess with the myc as there’s red #3 which has fungicidal properties. I’m still meaning to do a side by side test on this but going back through all my pics seems to back up that data. All the plates that did weird stuff was on those colors.

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Ohh really? Well that might be the case as it's the pink and purple plates that have been doing it. I usually use blue.

I followed this tek https://www.shroomery.org/forums/showflat.php/Number/26628234

And I use the exact same colouring as D3 has used.

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D3monic said:

I can send you a rusty whyte print for the aggravation/ setback you experienced if you like.

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And this is why you're a bad ass.

I should have gotten more pics of the magenta failures I had. Here's one. Myc grew downward inside of the agar before trying to expand and was extremely slow.
The orange worked just as good as the teal. I never tried the purple cause it also has red3.



This pic was 3 weeks after stabbing spores.


10 teal plates swabbed the day before germinated in 3 days.

Quote:

I'm gonna make up a stronger batch and a weaker batch tonight and test them.

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Clc420 said:

D3monic said:

It’s been found that the magenta and purple can mess with the myc as there’s red #3 which has fungicidal properties. I’m still meaning to do a side by side test on this but going back through all my pics seems to back up that data. All the plates that did weird stuff was on those colors.

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Ohh really? Well that might be the case as it's the pink and purple plates that have been doing it. I usually use blue.

I followed this tek https://www.shroomery.org/forums/showflat.php/Number/26628234

And I use the exact same colouring as D3 has used.

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D3monic said:

My question, is that even really myc digging down or is that bacteria. Small part of me thinks bacteria with hints of myc but I’m no expert in that regard.

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D3monic said:

My question, is that even really myc digging down or is that bacteria. Small part of me thinks bacteria with hints of myc but I’m no expert in that regard.

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nmd_myco said:

Clc420 said:

D3monic said:

It’s been found that the magenta and purple can mess with the myc as there’s red #3 which has fungicidal properties. I’m still meaning to do a side by side test on this but going back through all my pics seems to back up that data. All the plates that did weird stuff was on those colors.

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Ohh really? Well that might be the case as it's the pink and purple plates that have been doing it. I usually use blue.

I followed this tek https://www.shroomery.org/forums/showflat.php/Number/26628234

And I use the exact same colouring as D3 has used.

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I can support this info. Cultures do grow on the pink, but slow. Had to try for myself. Best growth is on Blue and Orange. Not su sure about purple yet...

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sporecap said:

sporecap said:

@xspak:
Very interesting! Did you sterilize the slide (if so, how) or just took one from a fresh pack?

Just did the poor man's version of the microscopy slide:

-Sterilize scalpel and cut a small well in a new agar dish (a bit off center)
-take a small transfer from the bacterial plate and put it in the well of the new plate
-sterilize scalpel again
-cut a large square agar wedge from the new plate and put it over the well to cover the transferred agar piece.

Will post results as soon as available.

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Here's an update on the agar sandwich method after 4 days. Growth speed was almost not affected, the myc seemed to have no problem to grow through the agar when being confined. In almost all plates it dominantly grew upwards and then emerged on top of the agar wedge, something which I did not expect. I thought it might be easier to squeeze through between the interface of the wedge and the agar plate, but it was definitely slower in that direction. The emerging agar was super thin and whispy, exactly like the translucent growth one sometimes gets from spores germinating on agar. Maybe this is a general feature of myc that actually growths through the agar? Once it stays on the surface it changes into a more rhizomorphic form?
I took transfers by scraping the top of the agar wedge with an inocculation loop, the amount of myc transferred was not detectable by eye (but I'm pretty sure there was some). Let's see what it does next.
If this proves to work well, I will implement it in my workflow: Spores to agar -> Transfer after Germination into Agar sandwich -> Transfer to normal plate.

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xspak said:

Thank you for the update sporecap ! Looks very good to me. My cover slides so far are also doing well. It will be interesting to see, if the growth after transfer looks better/same/more bad than the parent plate. In my experiment it looks much better - but that it not a surprise since the parent looked very bad.

I added another try where I put spores on agar - no streak, just one tiny point - and put a cover slide over it right away. Idea being that the fastest and most healthy children make it out of the trap first.

Also did some more cover slides for other plates, just to see what happens.

I really like these experiments. They might well lead to nothing, but there is always some learning and even if it is just learning what doesn't work.

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