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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: stareatclouds]
    #25567243 -

Good write up, thanks for that. Can anyone give me any more info of what kind of malt will work? Light malt, opposed to normal malt? I don't know much. Thanks.

Edit: https://www.woolworths.com.au/shop/productdetails/35176/saunders-malt-extract

This has some info in the pics about what's in it, would it be suitable?

Edited by Caster (10/25/18 06:41 PM)

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Caster]
    #25567356 -

Both light and regular malt will work, but don't use dark. This is what I use.


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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: stareatclouds]
    #25567841 -

stareatclouds said:
Both light and regular malt will work, but don't use dark. This is what I use.


Thanks a heap! That's good to know.

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: c10h12n2o]
    #25593391 -

Sorry if this is a stupid question, but i'm only on my third set of plates and I feel like my technique could definitely use some work, but i'm having a hard time finding the answers to my questions. Before you streak your plates, what's your method for removing the spores from the print? Do you add a drop or two of sterilized water to the print then swipe it? If so, do you stir them up in the water first before you swipe it? I put a little too much water on the print to loosen them from the foil on my last set, and it's now running on the surface of my Agar, so it's probably about to be contam city up in here. Also, the spores are in big clumps on my Agar instead of spread out (I used the zig zag method) Lessons learned I guess. That one was a PE6 print though, so I really hope I didn't trash those by accident :frown:

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: dmar]
    #25593403 -

Watch my video its in my links


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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: dmar]
    #25593408 -

c10 hasnt been around for awhile but heres what i do:

i always keep a few syringes of sterile water handy. making them is easy but with prints i take a sterile swab, wet the swab with the syringe of sterile water, then dab a tiny spot on the print to pick up spores. then swipe it to as many dishes as i need.

ive dropped whole chunks of spores onto agar. all blackish and gross looking but it will still germinate. souldnt ruin anything.


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:hypnotoad:
Making Liquid Culture :  Go insane with LC
Mushboy likes these teks : Making shroom tea
Dank Education

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: mushboy]
    #25595008 -

bodhisatta said:
Watch my video its in my links


I'll go check that out, thanks Bod.

mushboy said:
c10 hasnt been around for awhile but heres what i do:

i always keep a few syringes of sterile water handy. making them is easy but with prints i take a sterile swab, wet the swab with the syringe of sterile water, then dab a tiny spot on the print to pick up spores. then swipe it to as many dishes as i need.

ive dropped whole chunks of spores onto agar. all blackish and gross looking but it will still germinate. souldnt ruin anything.


So I PC a jar of water then take it into the SAB and open it up and fill the syringe, but I haven't tried a swab yet. When you say sterile swab, is that essentially just the Cotton tip applicators with the wooden tip that you can get at Walmart? Also, what's the advantage there when compared to using an inoculating loop?

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: bodhisatta]
    #25595022 -

bodhisatta said:
Watch my video its in my links


I just watched the video. You don't use any water whatsoever, so when you cool the loop down in the dish, some Agar sticks to it, which helps pull up the spores when you scrape them? :ooo:

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: dmar]
    #25596527 -

Why can't you use dark malt?

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: c10h12n2o]
    #25756721 -

Could I boil the agar in a pot instead of using the microwave? I’m assuming it’s fine just double checking

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Fireneeb]
    #25756738 -

yes you can. this thread is dead as shiznit though. you'll probably get more help posting in an active agar thread.

watch it closely though. the shiz likes to boil over and make a huge mess


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: verum subsequentis]
    #26262423 -

should inoculated agar be stored in darkness or on the shelf?

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Samalander]
    #26262932 -

Living things like light. Also, this thread is older than my great granny's panties. There are quite a few active agar threads going. Pop into one of those and you'll learn all kinds of cool shit.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: verum subsequentis]
    #26263384 -

great! thank you:thumbup:

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: Samalander]
    #26332454 -

tnx

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: verum subsequentis]
    #26463609 -

Hello friends, sorry for missing some questions,  I got super busy and had to take a hiatus from the forums for a while

The good news is that I'm back :smile:

So feel free to post any questions or share any new tricks you've learned

Much love


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: c10h12n2o]
    #26486623 -

Good to have you here again and thanks for taking the time to write this.
Wish I had read this sooner.
Two days ago I did my first plates and just swiped a Z with the inoculation loop. The thing is that I ran it through the agar not on top of it... but I think that will work out somehow.
Thanks again.

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: nosf3r4tu]
    #26486876 -

That'll happen sometimes. It's fine.


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: nosf3r4tu]
    #26487112 -

Happy to help :smile:

What was your agar recipe?

I really love the streaking technique posted here, REALLY speeds up the process of isolating cultures and cleaning contams. I've gotten an isolate in 3 transfers like this (!)


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C10's Agar Guide + Tips and Tricks | c10's Flow Hood Build Guide


"Partial knowledge is more triumphant than complete knowledge; it takes things to be simpler than they are, and so makes its theory more popular and convincing."

"Convictions are more dangerous enemies of truth than lies"
― Friedrich Nietzsche

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Re: c10's Agar Guide + Tips & Tricks (post #1000) [Re: c10h12n2o]
    #26487293 -

How do you know fur sure that it was an "isolate"


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Masters and slaves hunting clones        How not to isolate (it worked, but)


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