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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25889357 -

:homerdrool:


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HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tripdawg420]
    #25891380 -

From MS syringe after months of trying . Did about 4 transfers from this one, it being my first time ever didnt go as well as I hoped  :facepalm: but im sure it will work.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Mooshi97]
    #25891382 -

looks good

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Re: AGAR ENVY! (Anything and All things agar!) [Re: woofwoof]
    #25892678 -

Have this plate of Caerulescens from 1/31 that looks like itโ€™s trying to pin.  Took a couple transfers but was thinking about swabbing some myc for an LC also.  Think that would hurt my chance of getting it to pin?



Some other new species/phenotypes for me:

Ps. Semilanceata from Spain:



Redspore: (Agar was a little too soft when I swabbed it)



Pan. Aussieโ€™s from @Asura: (Used one plate for an LC)



Pan. Bisporus LC test plate looking promising.:-)



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Re: AGAR ENVY! (Anything and All things agar!) [Re: Brain Bulb]
    #25892889 -

Some sexy golden halo mycelium from clone..


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nobler Hino]
    #25893353 -

:whacker:


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HUSTLER
How U Survive This Life Everyday Resourcefully
epic GT mono tub
http://www.shroomery.org/forums/showflat.php/Number/17277772

wbs tek
http://www.shroomery.org/forums/showflat.php/Number/11525679
coir tek
http://www.shroomery.org/forums/showflat.php/Number/11917410
results :thumbup:

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Re: AGAR ENVY! (Anything and All things agar!) [Re: tripdawg420]
    #25893374 -

Whatโ€™s your agar recipe nobler?


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Wide Mouth 1/2 Pint No-Pour TEK
TC Teks & Links

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobbit]
    #25893950 -

I picked up the videos, looks great bobbit. thanks for the recommendation.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Just_A_Noob]
    #25894124 -

Just_A_Noob said:
Whatโ€™s your agar recipe nobler?


:whathesaid:


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Helpful Threads

The Shroomery Store

Tormato's Q&A Thread Post Questions Here or PM me!

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25894289 -

It's just 9g agar, 2.5g +or- brown rice rice flour and 500ml water. That may be mixed with diluted grain water, not sure.


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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nobler Hino]
    #25896894 -


Does this plate look like it needs anymore isolation to you guys? This is transfer #4


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Goddamn, Jimmie! This is some serious gourmet shit!

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Re: AGAR ENVY! (Anything and All things agar!) [Re: PortabellaKindaGuy]
    #25897557 -

Don't be concerned with isolation, but you want a clean culture... this looks like it's carrying bacteria IMO ... id let it grow to see if the white will overlap the thickened clear leading edge and fluff up a little... I like running MS tubs as genetically variant as possible and pick clones for isolation... isolating on agar prior to fruit is a crapshoot in the dark... You can even get super lucky and isolate a culture that absolutely will NOT fruit...

ALL my working cultures



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๐Ÿ„๐Ÿ’š๐Ÿ˜Ž

Ask Caps! (My Q&A Thread)
Caps' Comprehensive Cultivation Tek List

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25899373 -

Hi folks,

Just hoping to get a little advice on culturing what I think are P. subs.

Media: PDA + antibiotic
Starting material: spore print on paper

Method: I had a pretty spore print stuck on my pin up board since last season, and tried to 'inoculate' my media using 2 methods.

1. Cut small sections from the print and place directly on the plate.
2. Scrape spores onto the plate in little 'piles'

All of this was done in my living room, but I figure the antibiotic should be forgiving... but I have fungal contamination, the powdery green centres look bad maybe Penicillium?. There is some clear/white small growth (top right hand corner). I would like to know if I can cut this out and place it ontop of another PDA plate.



Also, I was wondering if you can recommend any good peer reviewed resources for  this type of work :smile:

Thanks in advance.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rullosaurus]
    #25900892 -

I've had limited success with p.sub wild-spore-on-paper (newsprint) to agar.

Firstly I'd recommend reducing the number of spores. Off foil, a dab on print to dab in agar is enough. With paper, rub 2-3 times, preferably with your loop cooled in agar first to encourage it to be sticky. Less is more.

Secondly I'd suggest increasing your sample rate. More plates = higher chance of success. You can use multiple sites per plate as you have, try 5-10 plates.

Thirdly, keep it cool. These fungi thrive in the outdoors, and keeping them cool, even refrigerated can be the head start they need over mould.


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The Official Kiwi Cultivators Thread

Official New Zealand woodlovers grow thread

โ€“โ€“โ€“โ€“โ€“    โ•ญโˆฉโ•ฎ(-_-)โ•ญโˆฉโ•ฎ    โ€“โ€“โ€“โ€“โ€“
The Official Kiwi DMT and MAOI Thread

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobbit]
    #25901323 -

Thanks so much for your reply Bobbit. I think I may try keeping a few prints on foil this season.

ok, got ya "less is more". I wonder if I can make a liquid spore suspension, and dilute a few times. Do you know if there is a liquid media that gives P subs an advantage over the green moulds? If not, I can just try the 'light dab' technique lol.

Sweet, I will try a few more plates next round, and place them in a cooler spot (at the moment, I have them in my laundry).

Have you tried using a section of the stipe on agar?

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Re: AGAR ENVY! (Anything and All things agar!) [Re: rullosaurus]
    #25901341 -

I did some stipe->agar->grains->cardboard->soaked woodchip->outdoor beds.

Being that this is my first season, I'm not sure they will fruit this season, though here's hoping.

As for spore solution, you could put one of your paper triangles (take a tiny piece) in a jar of sterilised water with 100ml or so. After a few days soaking, one drop per innoc. site per plate, in the cold.

Any nutrient water will work against you (I'm led to believe from lots of reading) agar+patience is your best bet.

Make more dishes that you intend to use in your initial set so as soon as you see myc, transfer to the empty plates . . .

Tissue from fruit gives you a very good chance of viable fruiting culture. In a SAB with a flame sterilised scalpel ready. . . Hold the base of the stipe in 2 hands and split without touching the freshly exposed sterile tissue. Cut a piece from the inside and flick onto waiting agar (only open the plate immediately before tissue transfer) close plate quickly. Wrap before removing from SAB.

You will see growth in 12-24 hours.

If you have a fresh syringe, and a fresh fruit, try the Josex Biopsy method. Use the stipe! One of my attempts was through the cap and ended up with multispore.


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The Official Kiwi Cultivators Thread

Official New Zealand woodlovers grow thread

โ€“โ€“โ€“โ€“โ€“    โ•ญโˆฉโ•ฎ(-_-)โ•ญโˆฉโ•ฎ    โ€“โ€“โ€“โ€“โ€“
The Official Kiwi DMT and MAOI Thread

Edited by Bobbit (03/28/19 03:58 AM)

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobbit]
    #25901974 -

Just started working with petris about a week ago and they are helping me to improve my technique because now I sometimes get contaminated plates. Anyway, here are some random B+ and Penis Envy plates I have going.

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Re: AGAR ENVY! (Anything and All things agar!) [Re: nulls]
    #25902148 -

Quick agar question.
When the myc hits the edge of the plate.
Can I still use them to tiger drop ect. 
No pour pasty plates.


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when god hands you lemons you,FIND A NEW GOD! 

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Re: AGAR ENVY! (Anything and All things agar!) [Re: Hoob33]
    #25902157 -

Is it clean? Yes!

Were the edges covered in bacteria? No!

Go to tiger drop, you got this.


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My Shoebox Factory Beginner's Tek | Shoebox Assembly Tek | Methodology/teks I use | How I Store Bulk Items

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Re: AGAR ENVY! (Anything and All things agar!) [Re: ShaperDreaming]
    #25902289 -

They look hella uniform even after taking 6 samples from it.
I have a few older dishes from a few days ago and to think
I been tossing all my old cultures to make fresh plates. Damnnnnnnn.


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when god hands you lemons you,FIND A NEW GOD! 

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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