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OfflineBobbit
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Registered: 05/30/18
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Pyrotek]
    #25923786 - 04/08/19 09:19 PM (3 months, 6 days ago)

Contamination can drive myc to pin.

Drop the pin on a fresh plate and see what happens.


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OnlineCaps McGeeS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobbit]
    #25923906 - 04/08/19 10:44 PM (3 months, 6 days ago)

They can still fruit: there have been lots of fruit from LOTS of bacterial spawn! Clone it! :shrug:


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OfflinePyrotek
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25925930 - 04/10/19 12:54 AM (3 months, 5 days ago)

Quote:

Caps McGee said:
They can still fruit: there have been lots of fruit from LOTS of bacterial spawn! Clone it! :shrug:




Is it worth trying to clone if the mycelium around it is bacterial? What's the best procedure for doing a transfer of a pin? Try to cut just the pin or the underlying agar as well?


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OfflineBobbit
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Pyrotek]
    #25926089 - 04/10/19 04:38 AM (3 months, 5 days ago)

Personally, I would
  • Drop the pin in distilled water for a few mins, to dilute any potential contams on the outside.
  • Pat dry with clean iso soaked paper towel.
  • Transfer pin to SAB with waiting plates.
  • With gloved alcohol washed hands, tear the pin down the middle.

  • Flame sterilize scalpel.
  • Cut a piece of tissue from the sterile inside of the pin freshly exposed.
  • Transfer piece to plate.
  • Rinse and repeat.

  • Wrap plates
  • Wait and watch


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OfflineHippiebait
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Registered: 01/06/19
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobbit]
    #25926141 - 04/10/19 05:42 AM (3 months, 5 days ago)

I just finished reading all 70 pages!! Amazing info!! Will be returning to this thread.


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OfflineHoob33
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Hippiebait]
    #25926153 - 04/10/19 05:58 AM (3 months, 5 days ago)

So I got a fully colonized plate edge to edge.
I wanna keep it going it’s my only one. Can I transfer from a section other than a leading edge?
Or just start freshhhh


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OfflineGrimsweeper
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Hoob33] * 1
    #25926180 - 04/10/19 06:58 AM (3 months, 5 days ago)

You can transfer from wherever you want but leading edges (should) be the most aggressive. Some people avoid the edges of a plate I think for fear of contaminants but if your plate is clean it's clean. :shrug:

Ps. Tamp clone looking sexy


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OnlineCaps McGeeS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Grimsweeper]
    #25926512 - 04/10/19 11:18 AM (3 months, 5 days ago)

I'd give it a shot... rinse it, no need for alcohol, and transfer the whole pin to avoid fiddling it more than necessary


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OfflineBobbit
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25926982 - 04/10/19 04:00 PM (3 months, 4 days ago)

Quote:

Caps McGee said:
I'd give it a shot... rinse it, no need for alcohol, and transfer the whole pin to avoid fiddling it more than necessary



I was suggesting treating like a wild specimen. I guess a contaminated plate would be much cleaner? :shrug:

Being overcautious again  :undecided:


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OfflineMoeMush
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobbit]
    #25927455 - 04/10/19 08:37 PM (3 months, 4 days ago)

Some t1 clone plates, first plate has a dark spot might be hard to see, and im not quite sure what it is, if its just from me not mixing my agar correctly, my thought is some contam i havent seen.


This is from another clone t1 made 2 1/2 days ago and i think looks the more promising of the two.

Thoughts?

Heres a close up of one of my plates just for shits and giggles



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Edited by MoeMush (04/10/19 08:54 PM)


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OfflineNowheremans
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Re: AGAR ENVY! (Anything and All things agar!) [Re: MoeMush]
    #25928301 - 04/11/19 10:30 AM (3 months, 4 days ago)

Purple mystic



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OfflinePsychedelicine
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Nowheremans]
    #25928446 - 04/11/19 12:11 PM (3 months, 4 days ago)



Took a syringe to 10 plates and this is the only thing that's grown so far... myc?


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InvisibleDephect
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Psychedelicine]
    #25928494 - 04/11/19 12:34 PM (3 months, 4 days ago)

Hoping to get a print in the mail so I can transfer that big RW agar pin and start some plates of a fresh cube strain.


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OfflineTormatoS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Dephect]
    #25931208 - 04/12/19 09:00 PM (3 months, 2 days ago)

Quote:

Pyrotek said:
I found an old pastyplate that has started to pin. I was under the impression that this particular plate was contamintated. However, since it is starting to pin I am now wondering if I'm wrong. Can contaminated plates pin at all? The mycelium doesn't look all that healthy to me but I'm no expert.



Got any pics? Cloning pins I just drop the pin on an agar plate and wait.

The more handling the higher likelihood of contamination....just gotta be ready to do some transfer to get a cleaner sample  :shrug:


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Tormato's Q&A Thread

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OfflinePsychedelicine
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25932523 - 04/13/19 03:38 PM (3 months, 1 day ago)

Different plate than I posted a few days ago. Still struggling to differentiate tomentose myc from mold.

Looks like myc to me...



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OfflineTormatoS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Psychedelicine]
    #25932541 - 04/13/19 03:50 PM (3 months, 1 day ago)

Quote:

Psychedelicine said:
Different plate than I posted a few days ago. Still struggling to differentiate tomentose myc from mold.

Looks like myc to me...






Looks like it to me as well...If it's mold it'll be about 3xs that size in 24 hours


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Tormato's Q&A Thread

Helpful Threads

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


Edited by Tormato (04/13/19 03:56 PM)


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OfflineBobbit
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25932706 - 04/13/19 05:33 PM (3 months, 1 day ago)

:whathesaid:
Mould grows so damned fast!

Transfer a leading edge and away!


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InvisibleBumholio
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobbit]
    #25932794 - 04/13/19 06:33 PM (3 months, 1 day ago)

Awww my APE myc is showing me some love:soproud:


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OfflineTormatoS
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Bumholio] * 1
    #25932928 - 04/13/19 07:52 PM (3 months, 1 day ago)

Quote:

Bumholio said:
Awww my APE myc is showing me some love:soproud:



Mush Love!


--------------------
Tormato's Q&A Thread

Helpful Threads

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


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OfflinePuduwoke
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Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25935393 - 04/15/19 04:42 AM (3 months, 10 hours ago)



Just some nice uniform growth of a tampanensis plate.


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