Home | Community | Message Board


FreeSpores.com
Please support our sponsors.

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Buy Bali Kratom Powder, Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder   Amazon Agar

Jump to first unread post. Pages: < First | < Back | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | Next >
OfflineBobbit
Even Stranger

Registered: 05/30/18
Posts: 257
Last seen: 7 hours, 47 minutes
Re: AGAR ENVY! (Anything and All things agar!) [Re: Pyrotek]
    #25923786 - 04/08/19 09:19 PM (16 days, 17 hours ago)

Contamination can drive myc to pin.

Drop the pin on a fresh plate and see what happens.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineCaps McGeeS
RustyWhyte supremacist
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 10,490
Loc: Out there man! Flag
Last seen: 3 hours, 51 minutes
Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobbit]
    #25923906 - 04/08/19 10:44 PM (16 days, 15 hours ago)

They can still fruit: there have been lots of fruit from LOTS of bacterial spawn! Clone it! :shrug:


--------------------

Ask Caps! (My Q&A Thread)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinePyrotek
Stranger
Registered: 06/02/18
Posts: 44
Last seen: 15 days, 13 hours
Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25925930 - 04/10/19 12:54 AM (15 days, 13 hours ago)

Quote:

Caps McGee said:
They can still fruit: there have been lots of fruit from LOTS of bacterial spawn! Clone it! :shrug:




Is it worth trying to clone if the mycelium around it is bacterial? What's the best procedure for doing a transfer of a pin? Try to cut just the pin or the underlying agar as well?


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineBobbit
Even Stranger

Registered: 05/30/18
Posts: 257
Last seen: 7 hours, 47 minutes
Re: AGAR ENVY! (Anything and All things agar!) [Re: Pyrotek]
    #25926089 - 04/10/19 04:38 AM (15 days, 10 hours ago)

Personally, I would
  • Drop the pin in distilled water for a few mins, to dilute any potential contams on the outside.
  • Pat dry with clean iso soaked paper towel.
  • Transfer pin to SAB with waiting plates.
  • With gloved alcohol washed hands, tear the pin down the middle.

  • Flame sterilize scalpel.
  • Cut a piece of tissue from the sterile inside of the pin freshly exposed.
  • Transfer piece to plate.
  • Rinse and repeat.

  • Wrap plates
  • Wait and watch


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineHippiebait
Stranger
Registered: 01/06/19
Posts: 183
Last seen: 1 day, 16 hours
Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobbit]
    #25926141 - 04/10/19 05:42 AM (15 days, 8 hours ago)

I just finished reading all 70 pages!! Amazing info!! Will be returning to this thread.


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineHoob33
Three toed sloth head yo
I'm a teapot User Gallery


Registered: 02/25/17
Posts: 952
Loc: Rain forest
Last seen: 38 minutes, 41 seconds
Re: AGAR ENVY! (Anything and All things agar!) [Re: Hippiebait]
    #25926153 - 04/10/19 05:58 AM (15 days, 8 hours ago)

So I got a fully colonized plate edge to edge.
I wanna keep it going it’s my only one. Can I transfer from a section other than a leading edge?
Or just start freshhhh


--------------------
when god hands you lemons you,FIND A NEW GOD! 


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineGrimsweeper
Mycooliogist
 User Gallery

Registered: 01/29/18
Posts: 1,728
Last seen: 1 hour, 37 minutes
Re: AGAR ENVY! (Anything and All things agar!) [Re: Hoob33] * 1
    #25926180 - 04/10/19 06:58 AM (15 days, 7 hours ago)

You can transfer from wherever you want but leading edges (should) be the most aggressive. Some people avoid the edges of a plate I think for fear of contaminants but if your plate is clean it's clean. :shrug:

Ps. Tamp clone looking sexy


--------------------
When you clean a vacuum cleaner, you are a vacuum cleaner.
                                     

Grimsweeper's not so simple AF All in One Vend-O-Matic Grow Chamber 5000
Grim's Groovy GrimGrains
ShroomCubes
Build yourself a Flow Hood in these 99 simple steps


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineCaps McGeeS
RustyWhyte supremacist
I'm a teapot User Gallery


Registered: 10/28/17
Posts: 10,490
Loc: Out there man! Flag
Last seen: 3 hours, 51 minutes
Re: AGAR ENVY! (Anything and All things agar!) [Re: Grimsweeper]
    #25926512 - 04/10/19 11:18 AM (15 days, 3 hours ago)

I'd give it a shot... rinse it, no need for alcohol, and transfer the whole pin to avoid fiddling it more than necessary


--------------------

Ask Caps! (My Q&A Thread)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineBobbit
Even Stranger

Registered: 05/30/18
Posts: 257
Last seen: 7 hours, 47 minutes
Re: AGAR ENVY! (Anything and All things agar!) [Re: Caps McGee]
    #25926982 - 04/10/19 04:00 PM (14 days, 22 hours ago)

Quote:

Caps McGee said:
I'd give it a shot... rinse it, no need for alcohol, and transfer the whole pin to avoid fiddling it more than necessary



I was suggesting treating like a wild specimen. I guess a contaminated plate would be much cleaner? :shrug:

Being overcautious again  :undecided:


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineMoeMush
Stranger
Male

Registered: 01/26/19
Posts: 71
Last seen: 1 hour, 31 minutes
Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobbit]
    #25927455 - 04/10/19 08:37 PM (14 days, 18 hours ago)

Some t1 clone plates, first plate has a dark spot might be hard to see, and im not quite sure what it is, if its just from me not mixing my agar correctly, my thought is some contam i havent seen.


This is from another clone t1 made 2 1/2 days ago and i think looks the more promising of the two.

Thoughts?

Heres a close up of one of my plates just for shits and giggles



--------------------


Edited by MoeMush (04/10/19 08:54 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineNowheremans
I'm a teapot


Registered: 10/06/18
Posts: 118
Loc: on the spiral
Last seen: 3 hours, 4 minutes
Re: AGAR ENVY! (Anything and All things agar!) [Re: MoeMush]
    #25928301 - 04/11/19 10:30 AM (14 days, 4 hours ago)

Purple mystic



--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinePsychedelicine
Padawan
I'm a teapot User Gallery


Registered: 02/22/19
Posts: 76
Loc: Dimension C-137
Last seen: 17 hours, 18 minutes
Re: AGAR ENVY! (Anything and All things agar!) [Re: Nowheremans]
    #25928446 - 04/11/19 12:11 PM (14 days, 2 hours ago)



Took a syringe to 10 plates and this is the only thing that's grown so far... myc?


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleDephect
Male User Gallery


Folding@home Statistics
Registered: 08/10/07
Posts: 1,620
Re: AGAR ENVY! (Anything and All things agar!) [Re: Psychedelicine]
    #25928494 - 04/11/19 12:34 PM (14 days, 2 hours ago)

Hoping to get a print in the mail so I can transfer that big RW agar pin and start some plates of a fresh cube strain.


--------------------




Post Extras: Print Post  Remind Me! Notify Moderator
OfflineTormatoS
It’s OK!! I’m a Limo Driver!! 😁
Male User Gallery


Folding@home Statistics
Registered: 07/01/17
Posts: 4,109
Loc: Montagar
Last seen: 1 day, 2 hours
Re: AGAR ENVY! (Anything and All things agar!) [Re: Dephect]
    #25931208 - 04/12/19 09:00 PM (12 days, 17 hours ago)

Quote:

Pyrotek said:
I found an old pastyplate that has started to pin. I was under the impression that this particular plate was contamintated. However, since it is starting to pin I am now wondering if I'm wrong. Can contaminated plates pin at all? The mycelium doesn't look all that healthy to me but I'm no expert.



Got any pics? Cloning pins I just drop the pin on an agar plate and wait.

The more handling the higher likelihood of contamination....just gotta be ready to do some transfer to get a cleaner sample  :shrug:


--------------------
Tormato's Q&A Thread

Helpful Threads

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


Post Extras: Print Post  Remind Me! Notify Moderator
OfflinePsychedelicine
Padawan
I'm a teapot User Gallery


Registered: 02/22/19
Posts: 76
Loc: Dimension C-137
Last seen: 17 hours, 18 minutes
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25932523 - 04/13/19 03:38 PM (11 days, 23 hours ago)

Different plate than I posted a few days ago. Still struggling to differentiate tomentose myc from mold.

Looks like myc to me...



Post Extras: Print Post  Remind Me! Notify Moderator
OfflineTormatoS
It’s OK!! I’m a Limo Driver!! 😁
Male User Gallery


Folding@home Statistics
Registered: 07/01/17
Posts: 4,109
Loc: Montagar
Last seen: 1 day, 2 hours
Re: AGAR ENVY! (Anything and All things agar!) [Re: Psychedelicine]
    #25932541 - 04/13/19 03:50 PM (11 days, 22 hours ago)

Quote:

Psychedelicine said:
Different plate than I posted a few days ago. Still struggling to differentiate tomentose myc from mold.

Looks like myc to me...






Looks like it to me as well...If it's mold it'll be about 3xs that size in 24 hours


--------------------
Tormato's Q&A Thread

Helpful Threads

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


Edited by Tormato (04/13/19 03:56 PM)


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineBobbit
Even Stranger


Registered: 05/30/18
Posts: 257
Last seen: 7 hours, 47 minutes
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25932706 - 04/13/19 05:33 PM (11 days, 21 hours ago)

:whathesaid:
Mould grows so damned fast!

Transfer a leading edge and away!


Post Extras: Print Post  Remind Me! Notify Moderator
InvisibleBumholio
Pretty fly for a white guy.
 User Gallery


Registered: 07/23/18
Posts: 2,826
Loc: Shroomsville
Re: AGAR ENVY! (Anything and All things agar!) [Re: Bobbit]
    #25932794 - 04/13/19 06:33 PM (11 days, 20 hours ago)

Awww my APE myc is showing me some love:soproud:


--------------------
Trade List





“Great things may come to those who wait, but only the things left by those who hustle.”


Post Extras: Print Post  Remind Me! Notify Moderator
OfflineTormatoS
It’s OK!! I’m a Limo Driver!! 😁
Male User Gallery


Folding@home Statistics
Registered: 07/01/17
Posts: 4,109
Loc: Montagar
Last seen: 1 day, 2 hours
Re: AGAR ENVY! (Anything and All things agar!) [Re: Bumholio] * 1
    #25932928 - 04/13/19 07:52 PM (11 days, 18 hours ago)

Quote:

Bumholio said:
Awww my APE myc is showing me some love:soproud:



Mush Love!


--------------------
Tormato's Q&A Thread

Helpful Threads

"Lately it occurs to me what a long, strange trip it's been." ~ Grateful Dead

Before you start...Do you have a Pressure Cooker and a Dehydrator? I highly recommend getting both!


Post Extras: Print Post  Remind Me! Notify Moderator
OnlinePuduwoke
mushroom enthusiastic
Male User Gallery

Registered: 06/25/17
Posts: 462
Loc: Europe
Last seen: 4 minutes, 2 seconds
Re: AGAR ENVY! (Anything and All things agar!) [Re: Tormato]
    #25935393 - 04/15/19 04:42 AM (10 days, 9 hours ago)



Just some nice uniform growth of a tampanensis plate.


--------------------


Post Extras: Print Post  Remind Me! Notify Moderator
Jump to top. Pages: < First | < Back | 53 | 54 | 55 | 56 | 57 | 58 | 59 | 60 | 61 | 62 | 63 | 64 | 65 | 66 | 67 | 68 | 69 | 70 | 71 | 72 | 73 | Next >

Shop: PhytoExtractum Buy Bali Kratom Powder, Kratom Powder for Sale, Maeng Da Thai Kratom Leaf Powder   Amazon Agar

Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation

Similar ThreadsPosterViewsRepliesLast post
* buying agar - help sinisha 2,041 6 03/31/06 07:49 AM
by FractalXplora
* cheapest glass petri dishes, agar, dextrose, etc Fluxburn 3,268 8 02/14/05 09:57 AM
by Roadkill
* Potato Dextrose Agar X Malt Extract Agar Alegalize 2,637 8 02/25/05 01:03 PM
by agar
* test tubes agar fungophiliac 1,468 7 12/13/04 11:34 PM
by fungophiliac
* LOOK! Big AGAR problem. Ever seen this? Not I. need advice BAD! agentstone 1,406 10 11/17/05 02:42 AM
by hills
* Agar and the global economy Dem_Bones 853 4 03/01/06 11:08 AM
by Dem_Bones
* Agar question WormholeSurfer 622 3 10/29/05 09:10 PM
by agar
* Agar's Home Made Compost recipe for bulk substrates
( 1 2 3 4 all )
agar 15,229 74 05/08/06 10:03 PM
by Snaggletooth

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Roadkill, Magash, Shroomism, george castanza, RogerRabbit, Citric, total, FooMan, 13shrooms, stonesun, cronicr, PussyFart, Tmethyl, Pastywhyte, bodhisatta
18,640 topic views. 29 members, 112 guests and 8 web crawlers are browsing this forum.
[ Print Topic ]
Search this thread:
Buy-Kratom.us
Please support our sponsors.

Copyright 1997-2019 Mind Media. Some rights reserved.

Generated in 0.034 seconds spending 0.004 seconds on 17 queries.