|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
|
Mycelium Water, A Tribal Container [beta version] 6
#21927954 - 07/11/15 08:52 AM (8 years, 6 months ago) |
|
|
Mycelium Water, A Tribal Container  Blending, pouring and/or making syringes with one jar

The goal is to make an eberbach like jar with a self-healing injection port to suck LI into syringe and a pour system for the pf-slurry. I like the pour plug system to pour LI AND SLURRY because its very easy to use and can be a bit more precice when i pour into grain jars and feel safer with the smaller opening compared to pouring strait from the jar, especially when i noc 30-40 jars with a batch
This is the beta version, some tweakage may come.Jar Construction i'll mostly let the picture talk Things you'll need: - ball sip and straw lids
- ball freezer jars 16oZ or similar pp5 container
- heavy duty self-healing injection ports
- an oster blender blade with the seal
- 2" hole saw
- 1/2" drill bit
- small saw or dremel
- a small magnet or some type of screw for the plug(be creative;))
- silicone
- sandpaper
Place the lid offset near the pour hole to be closest of the side of the jar to reduce wastage, (maybe optional)  The white lid in this pic is not the one i used, i cutted it too small See where i made the lines, there is a kind of a rim, you want to keep the most of this rim because this is what will hold the white sip'n'straw lid    1/2" spade drill bit to make the SHIP holes, for the plug you'll have to make it a bit bigger for easy pulling  Sand the edge so the silicone stick well, i place the lid on a tape roll and apply the silicone, wait until it dry and make the other side   Hole saw 2", mine isn't very good so i predrill to avoid cracking and again, dont forget to sand the base before applying silicone   also dont forget to sand the oster blade, because thats what will happen if not one PC cycle and it peel off..  so sand where you will apply silicone,   Once its mostly dried, i put the seal on the bottom for stability     A small magnet is glued to one of the SHIP (the plug)  In my glove, i place a small knot or washer hardware or even a dime, anything magnetic enough so the plug stick to my finger to pull it off thanx tourrat for the magnet idea    Once everything is completely dry, put water in it to test the sealing, the only place you should see leaks is from the pour plug but its ok because its for immediate use  using the container Fill at 1/2 or 2/3 of the container with tap water, depending on how much inoculum you want, screw tightly, cover with foil, pc for 30min and follow your favorite liquid/slurry inoculant tek like these 3 Day Quart Colonization: Slurry Liquid Spawn or InoculantLiquid Inoculant ( NEW version)MY MudaFukan BOTTLE TEK( as easy as it gets)Updated Bottle Tek Pouring with the container
This is an over colonized AA+ master jar, i was unable to break the chunk for g2g so i though it was good candidate for a slurry!
i had hard time because the small knot in my glove had not enough magnetic force to be able to pull the plug properly i'll make the hole slightly bigger and use something like a dime next time. Results! 48 hrs after inoculation of WBS, 72 hours, 84hrs(shaking), spawned on day 5     tub 1     Tub 2, i had a small contam spot in top but surgery went well       Jar typesNow for the type of jar you are not limited with those model but i highly suggest you this model with a screw lid:   over this model with a snap lid:  There is also these Blender Bottles that look perfect for the job!  Quote:
Dhearic said:
,  , , 

So this is it for now! Good luck in your Liquids inoculants and slurries adventures!!

Brought to you by THE TRIBE
Edited by cArcace-x (10/16/15 10:49 AM)
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x] 1
#21927969 - 07/11/15 08:57 AM (8 years, 6 months ago) |
|
|
Snap Lid Build
So Carc suggested the first container. Unforunately, I didn't have it available in my area. So I had to use the other one with the snap lid the only ones available near me at walmart, big lots and grocery store were the snap lid. i know they aren't recommended but i took some close up pictures of them. the lids have a real nice seal. the smaller 8oz were too small for the blender assembly. ill use those for food. but the 16oz almost fits the metal band lid for a regular mouth half pint on the bottom. it fits on the blender base! ill go ahead and build it. and if it doesn't fit on the base, ill use it for LI with plates. also the 8oz Ball brand are too narrow for the white lids to fit on. But they are nice little containers. i think this should work. the seal is tight and there is enough clear plastic on the lid to drill out and rtv the metal blade assembly to. i messed around a little bit and put the lid and pulled it off several times. i think i know where the complaint comes from. if you push one side down without doing it evenly it pushes air out. if you press around the top with a few fingers on all sides it seals perfectly. the bottom of this one appears to need a 2.25" hole saw. im excited to try this out soon.
   i also found a pp5 with a wide lid on it that is pp5 at big lots. although the base is too wide for an oster i think the drill bit will work with blade attachment in the bottom. i might mess around with both of these. the lids do have a really nice seal on them. but it appears to be a pain in the ass to try and cut into it. im going to order the ones you suggest for the build. i have these until i return them so i thought i would at least take some close up images. i was also able to pickup some pickling lime as the grocery store had a nice little section for canning.
 
a tiny bit of pressure can crack these plastic jars. i didn't even use the drill and put a little pressure and the bottom cracked. so on the 2nd jar i used the small bit and worked my way up. it worked perfectly.
i drilled small guide holes so that i could fit the saw in and then sawed evenly and sanded afterwards. here is before the glue dries.
glue ha set and now im just waiting until tomorrow to put it together. i had to make a slight modification with this lid. it wouldn't get the proper seal if i cut anymore towards the edge of the jar. so i had to fit the white lid into the rim of the green seal. i would have had to cut too far on this lid that it would effect the seal. so i kept it in the middle. i made two white lids. one with two blue plugs. one with 1 plug and i was siliconing in the small hole. i plan on alternating it depending on which method i use. if i want to suck it up into a syringe i will use the lid with the two blue ports. if i want to pour. ill use the lid with 1 blue port. im fairly certain this will work well. the only issue is the clear plastic on the lid and the green soft plastic. where the seal meets its extremely tough to cut through. i kept a small portion of the outside clear plstic to keep that seal and drop the white lid in.

i let it dry and sealed the inside of the lid. fin. ill test it tomorrow on the blender.

look what i got. these little magnets are stronger than the magnetic vent covers.

found one lid with this ship. glued the magnet in. i could barely separate those small magnets. extremely strong. i accidentally broke the lid by trying to drill this ship hole bigger. so i had to silicone it again.

so this worked out perfectly. i had to reseal it a few times. as i was running low on silicone when i made it. today it worked perfectly. i did an LI inoculated 15 quart jars from an agar plate of syzygy from an old print. some sick magnet action!
after a blend


Here are my first 3 tub trials from using the tribal container. These are multispore. From Syzygy Multispore Grow 2015
4 days after inoculation using my tribal container!

day 13 i spawned the jars right after that.

3 Tub Grow
  
    
 
  
 
Edited by eatyualive (07/13/15 09:52 PM)
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive] 1
#21927992 - 07/11/15 09:02 AM (8 years, 6 months ago) |
|
|
Regular mouth half pint jar lid build
about to go ape shit on some liquid inoculants. built a regular mouth 1/2 pint backup for my tribal container.

1/4" guide and 1/2" drill bit

1/4" guide hole

1/2"hole
\
used the 1/2" hole to evenly drill around the hole to widen the hole so that the ship can sit. but loose enough so that when the magnet on the top is used with a glove it can easily be pulled out for a transfer.
  
Regular mouth Tribal Build
This lid will fit any regular mouth 1/2 pint, pint or quart jar. This comes in really handy if you plan on using your blade assembly without installing it on the bottom of a container. you can pressure cook this lid wrapped in foil at the same time you pressure cook your 1/2 pint, pint or quart jar half full of water. I will be using the 1/2 pint for small Liquid inoculations, the pint jar for larger grain slurry inoculations and the quart jar for pf jar slurries. Each jar has a specific use. This is in addition to the Tribal Container and the Larger Tribal Container.
   
siliconed the inside ring.
 sanded the edge of the lid with sand paper. also added a small bead of silicone to the small suction hole that is built into the cup.
after the silicone was left to set for about 10 minutes. the lid was pressed against the silicone. the silicone was sealed on the inside and outside of the lid.

Taller Higher Volume Container Build
the only downside i see is height of SAB. Also when you get to the end it might be difficult flipping it over 180%. But you can fit twice the volume of LI into it. The lid is somewhat loose. Enough that its easy to push down and open. However, this might be an issue when pressure cooking. I will likely use foil over the lid or micropore tape to hold the lid in place while pressure cooking.
This item was 8$ from walmart. The plastic is sturdy.
 
1/4" guide hole drilled.

3" hole saw drilled.
trimmed around the edge with an exacto.

sanded down with sand paper. blade assembly fits perfect. 
rtv 8" container

testing for any leaks tonight.

tested no leaks

Edited by eatyualive (07/23/15 11:12 PM)
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive] 1
#21927993 - 07/11/15 09:03 AM (8 years, 6 months ago) |
|
|
Liquid Inoculant and GS2G(Grain Slurry to Grain Transfer) Below
Now normally I was using a 2:1 grain to water ratio for these grain slurries as liquid inoculant. However, I wanted to try a smaller volume of grain to see how far a quart jar really can be expanded. This example shows how a GLC(Grainwater Liquid Culture) was basically extracted from a spoonful of grain. Once blended the mycelial water was poured into receiving quart grain jars for transfer. None of the grains from the GLC jar were poured into receiving jars this time. My normal GS2G method involves making more of a mud consistency blend and pouring the grains and blended water into the receiving jars.
One spoonful of a colonized quart jar was used per pint of water for the Tribal Slurry(GS2G) illustrated below. 1 plate to 1/4 pint of water was used for the Liquid Inoculant below.
So my GLC and LI jars are almost finished. The LI jars won slightly. I had to shake both because they were taking forever(more than 4 days). Im spoiled by slurry and the 2:1 grain to water slurries. For pure mycelial water, the colonization was as fast as the LI from a grain jar. 9 days full colonization. This is slow on my end for g2g.
Grain slurry and pf jar slurry are take half the time to colonize jars wothout a shake. The LI and GLC took 9 days with a shake on day 5 to colonize 100%. Although ive compared them several times. I will still continue to do so in this thread.
Day 1-9: Only 20 quarts were used with LI. 30 used with the GLC. the jars on the right of the colonized quart are a Syzygy clone. Jars on the left are an amazon clone. The quart jars were all shaken on day 5.
      
GLC Amazon
    Day of spawning

LI Syzygy
     
Liquid Inoculant: 1 pint water to 1 plate agar
A regular mouth quart jar was fitted with a blade assembly and filled with 1 pint of water. The Tribal lid for this jar was wrapped in foil and pressure cooked for 20 minutes at 15psi. The plate was dropped in the quart jar and blended for 2(3-5 second bursts). Taken back into the SAB. Then the lids were switched for pouring.
Normally, I used 1/4 pint of water per plate with the LI. This volume of water was 4 times the normal volume. In this trial the LI showed leap off on the 2nd day. This is Penis Envy.

to be updated...
Quote:
Normal procedure for GS2G transfers. Now incorporating the use of the Tribal Container to make the entire process streamlined, easy and less messy.
SLURRY TYPE 2:
GS2G Transfer(Grain Slurry to Grain jar transfer): wbs grain jar blended and poured into other grain jars. A ratio of 1 pint grain spawn to 1/2 pint of water was used for 50 quarts of colonized grain.
Do all of this as sterile as possible. Research on sterile technique is assumed prior to this.
1. Fill a pint jar half full of distilled water and place the blade assembly on it. Pressure cook for 20 mins at 15psi. Allow jar to cool. 2. Prepare your clean area(Shmuvbox, SAB, Flowhood) and your substrates and tubs. Setup for spawning. 3. Using a phonebook, break up the grain inside the jar. 4. In your SAB, Take the colonized pint of grain loosen the lid. Loosen the lid of the sterile water pint jar. 5. pour water from water pint into grain pint. 6. place blade assembly lid on grain pint and water pint jar. 7. Blend on your oster blender base. Hold the jar in place with your hand while blending. I blend about 30 seconds. 8. In your SAB, Pour the grain slurry into your quarts jars. You pour by eye about 1 tablespoon volume of slurry per Quart Grain Jar. 9. Wait 3-4 days until full colonization. Give it a day or two more and then your ready to spawn your tubs. You can shake your grain a day before spawning to test for any contamination.
Regular Mouth Lids
 
Quarts and Pints(Pints will each be used as masters to slurry 100 more quarts each. In the last picture you can see all the colonized pints and 1 quart that i slurried. all the colonized jars are on the last bottom picture. each one of those pints will be used as a master for more than 30 jars at a time)judging from experience, each pint can do at least 200 quart jars of grain. i easily did 30 tonight and had to throw out 2/3rds of the pint jar of grain leftover.
   
SLURRY VS LIQUID INOCULANT
The Liquid inoculant(texan yellow cap clone-3rd transfer plate) was inoculated on 8-11-2015. The slurry(Thai Lipa Yai multispore pf jar) was inoculated on 8-16-2015. The Liquid inoculant is a fresh clone Texas Yellow Cap plate from agar. The slurry is a 1 years old multispore Thai Lipa Yai from a pf jar that had been sitting in the fridge from 2014.
As you can see the first image was taken on 8-18-2015. 2 days after the slurry was inoculated. The last picture was taken today. So the LI took roughly 8 days to colonize 99%. The slurry in 3 days has almost caught up with the LI. Neither set of jars has been shaken since inoculation.
If you zoom in you can see the tape label. The LI jars are to the left of the LI label. The Slurry jars are to the right of the label. So half the speed on grain. This is consistent for both LI and Slurry with a multitude of tests.
A little background on these cultures. the thai culture is the fastest growing cube culture i have. tex is 2nd fastest. very close in speed. but the thai is multispore and the tex is a clone. the thai pf jar sat in the fridge for over a year and still caught up to the fresh tex clone culture.
check it out. day 4 on the slurry almost done. caught right up to the LI that had a 5 day head start.
  
Edited by eatyualive (08/20/15 06:21 PM)
|
Darkhome
•Shaman•N•Training•



Registered: 07/10/15
Posts: 517
Loc: United States
Last seen: 7 years, 1 month
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#21928364 - 07/11/15 11:05 AM (8 years, 6 months ago) |
|
|
You guys are Amazing! New ideas are what make advances in this hobby possible!
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
|
cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: Darkhome]
#21930439 - 07/11/15 07:24 PM (8 years, 6 months ago) |
|
|
Quote:
Darkhome said: You guys are Amazing! New ideas are what make advances in this hobby possible! 
No YoU are amazing!!!
haha thanks mate!
eatyualive... You tha man!!! 
The video of the inoculation in the OP been done 2 days ago and its showing good recovery. I will update the grow to the OP as things progress. Other member's built and other options/ideas will also be added to the OP as things move on
cheers

--------------------
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#21933034 - 07/12/15 11:25 AM (8 years, 6 months ago) |
|
|
look what i found. I think this is the one Dhearic found. I will give it a try. It's huge she said.
 
the only downside i see is height of SAB. Also when you get to the end it might be difficult flipping it over 180%. But you can fit twice the volume of LI into it. The lid is somewhat loose. Enough that its easy to push down and open. However, this might be an issue when pressure cooking. I will likely use foil over the lid or micropore tape to hold the lid in place while pressure cooking.
This item was 8% from walmart. The plastic is sturdy.
Edited by eatyualive (07/12/15 11:34 AM)
|
cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#21934536 - 07/12/15 06:05 PM (8 years, 6 months ago) |
|
|
 keep it updated man!!
almost 48hrs after inoculation:
--------------------
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#21934890 - 07/12/15 07:55 PM (8 years, 6 months ago) |
|
|
|
OPB
Wanderer

Registered: 05/30/15
Posts: 198
Last seen: 2 years, 10 days
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#21934915 - 07/12/15 08:02 PM (8 years, 6 months ago) |
|
|
That's the most serendipitous use of a "blender bottle" I've ever seen.
-------------------- "Wasn't that fun"
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: OPB]
#21939415 - 07/13/15 09:31 PM (8 years, 6 months ago) |
|
|
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#21940001 - 07/13/15 11:44 PM (8 years, 6 months ago) |
|
|
What's the point of the magnets? Is it so you can take the plug out with a single finger? Any other use?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#21940082 - 07/14/15 12:04 AM (8 years, 6 months ago) |
|
|
single finger
|
cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: azur]
#21940507 - 07/14/15 03:25 AM (8 years, 6 months ago) |
|
|

one can also do a 2 finger thing if interested

--------------------
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#21940689 - 07/14/15 05:32 AM (8 years, 6 months ago) |
|
|

testing for any leaks tonight.
|
cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive] 2
#21940728 - 07/14/15 05:54 AM (8 years, 6 months ago) |
|
|
i really like the lid, look easy to pour with it too i though about something like that at the very beginning but i havent found any good pp5 bottle like that at the time so i went for the jar but havent looked at walmart..
found the original plan... lol i though it wouldn't be good for slurries but just for LI.... until i tried! 

thats some microsoft paint skillz! hahah
--------------------
|
Dhearic


Registered: 01/24/14
Posts: 846
Loc: Neverland
Last seen: 9 months, 3 days
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x] 1
#21944420 - 07/14/15 10:52 PM (8 years, 6 months ago) |
|
|


Doesn't leak and blends myc like a charm
--------------------
Credit where credit is due.
|
cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: Dhearic]
#21945891 - 07/15/15 05:26 AM (8 years, 6 months ago) |
|
|

Me want this model!!!!!
Rock-on Dhearic!!!
--------------------
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#21946120 - 07/15/15 07:16 AM (8 years, 6 months ago) |
|
|
I'll take 2. Where do I send my $12.95 + $4.99 S/H ?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: Dhearic]
#21956930 - 07/17/15 04:44 PM (8 years, 6 months ago) |
|
|
Quote:
Dhearic said:


Doesn't leak and blends myc like a charm 
nice! im going to build a small one soon also. im testing both this weekend. i should have at least 50 quarts ready by then!
time to get to work. ill update the op post i have with the jars colonizing once it becomes available.
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#21957338 - 07/17/15 06:17 PM (8 years, 6 months ago) |
|
|
tested and about to be used. no leaks.
|
Darkhome
•Shaman•N•Training•



Registered: 07/10/15
Posts: 517
Loc: United States
Last seen: 7 years, 1 month
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#21957354 - 07/17/15 06:20 PM (8 years, 6 months ago) |
|
|
Quote:
eatyualive said: tested and about to be used. no leaks.

You tha man!
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
Edited by Darkhome (07/17/15 06:21 PM)
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: Darkhome]
#21957375 - 07/17/15 06:26 PM (8 years, 6 months ago) |
|
|
actually Dhearic found that one, i just copied him. his build is much smoother. i can't silicone for shit.
|
Darkhome
•Shaman•N•Training•



Registered: 07/10/15
Posts: 517
Loc: United States
Last seen: 7 years, 1 month
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#21957384 - 07/17/15 06:29 PM (8 years, 6 months ago) |
|
|
I'm with you...can't silicone for shit myself...lol Props to Dhearic...for sure
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
Edited by Darkhome (07/17/15 06:31 PM)
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: Darkhome]
#21957502 - 07/17/15 07:04 PM (8 years, 6 months ago) |
|
|
I still seriously wanna buy one for a fair price.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
Darkhome
•Shaman•N•Training•



Registered: 07/10/15
Posts: 517
Loc: United States
Last seen: 7 years, 1 month
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#21957518 - 07/17/15 07:09 PM (8 years, 6 months ago) |
|
|
Quote:
maddchef said: I still seriously wanna buy one for a fair price.
Me too... If they ever go on the market... Just tell me where to send a money order to
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: Darkhome]
#21964008 - 07/19/15 09:41 AM (8 years, 6 months ago) |
|
|
so ive actually gone ahead and resealed every container with the red rtv silicone. this other clear rtv silicone wasn't holding the seal tight when pressure cooked. it would push open leaks. so the entire tribal container was re sealed.
on the larger blender bottle container, the inside metal base of the blender blade had a small leak in it. so that was rtved. going to give it a go in a few days. wanted to double check and make sure things were air tight. believe it or not the silicone seal was fine 

my shitty craftsmanship with silicone probably didn't help. it looks like a horror flick up in this bottle. the edge of the bottle and the edge of the lid were so close it was difficult to connect so clamps had to be used to keep them together while the silicone set.
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#21964156 - 07/19/15 10:14 AM (8 years, 6 months ago) |
|
|
I made the blender part last night to one of those snap lids 16oz ball freezer jars. I should really buy a heavy duty port to put in it but probably won't wait that long and just make my own ship.
I didn't make mine with a pour spout simply because I don't do slurries so I'll just stick it with a few 60cc syringes.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#21964651 - 07/19/15 12:02 PM (8 years, 6 months ago) |
|
|
i didn't add an extra port on mine. but my tribal container is specifically for pouring Liquid inoculants. The ship on my tribal container is covered by a magnet for pouring and used as a plug only. if your using the ship on yours to make syringes, it sounds like you have what you need.
Edited by eatyualive (07/19/15 12:38 PM)
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#21964706 - 07/19/15 12:24 PM (8 years, 6 months ago) |
|
|
so i had to use my 1/2 pint lid build Liquid Inoculation jar on this run. One plate with 1/4 pint water inoculated 20 quart jars.
regular mouth 1/2 pint lid build

I also used GLC by taking one spoonful of colonized spawn, putting it in a regular mouth quart jar with oster blade assembly. blended it for about 3-5 seconds. then poured that into 30 quart jars.
GLC jar build.

you can see on the left of the colonized quart are the Liquid inoculated jars on the right are the Grain Liquid Culture jars. I was going to do a side by side and see which one is faster. I think the LI will be as the GLC takes a little longer to establish. But, Only one spoonfull of mycelia was used illustrated in the op in carc's video.

i have to say carc. i enjoy the way the tribal container pours better than the 1/2 pint and quart jar regular mouth builds. its smoother and the angle of the pour spout works perfect with that notch out. i found this 1/2 pint lid build to often spill the LI on the side before it went into the jar. it worked, but was not as smooth.
and CONGRATS! on the 5 day spawning from inoculation. that is the way it is done!
Edited by eatyualive (07/19/15 12:35 PM)
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#21964811 - 07/19/15 12:48 PM (8 years, 6 months ago) |
|
|
Quote:
cArcace-x said: i really like the lid, look easy to pour with it too i though about something like that at the very beginning but i havent found any good pp5 bottle like that at the time so i went for the jar but havent looked at walmart..
found the original plan... lol i though it wouldn't be good for slurries but just for LI.... until i tried! 

thats some microsoft paint skillz! hahah 
yeah some real microsoft paint skillz right there! haha
|
cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#21966890 - 07/19/15 07:43 PM (8 years, 6 months ago) |
|
|
spawn-run day 4(day 9 from spawn inoculation ) another case where we can see the importance of mixing the spawn/substrate very well.. tub 1

tub 2

Quote:
maddchef said: I made the blender part last night to one of those snap lids 16oz ball freezer jars. I should really buy a heavy duty port to put in it but probably won't wait that long and just make my own ship.
I didn't make mine with a pour spout simply because I don't do slurries so I'll just stick it with a few 60cc syringes.
yeah man sound great build for your need is what is cool with DIY's for example NumeroEno who want to use it for LC, he just have to put a SHIP. He would sterilize his water with nutrient, then add the wedge and blend.. suck it up into syringe when ready, the blade is stainless steel anyway so it won't rust in the LC. it is also a good use of the jar but if you do have a pour plug, use it right away because its loose and contam will enter from the plug hole if you don't pour, fuck the plug!
Quote:
eatyualive said: and CONGRATS! on the 5 day spawning from inoculation. that is the way it is done!
i'm very happy with the result i must say why i think grain spawn slurry is faster than pf slurry?... because i havent blend as much as i would do for pf-slurries. Colonised WBS is much more easier to blend compared to the pf-cakes slurries i have done, so less damages is made to the mycelium and the recovery time is shorten..
--------------------
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#21966904 - 07/19/15 07:46 PM (8 years, 6 months ago) |
|
|
Hey wait a fuggin mi the. You're slurrting colonized grain? Link me to said thread if it exists. Any slower or faster than LI and lc?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#21966921 - 07/19/15 07:49 PM (8 years, 6 months ago) |
|
|
yes your right about using less is more. i used one spoon full on this last round! that spoon full inoculated 30 quart jars. the rest of that colonized quart is going to be spawned to a tub tomorrow. this is one reason i keep pint size grain masters like the images below. i still get faster colonization speed with the pf jar slurries over the grain slurries on average. but maybe thats a matter of tweaking a few things like what you have done here. i did the exact way in the video.
ill break these pints up with a phone book and dump it into my blender jar for an easy inoculation for more than 50 plus grain jars. that one pint will probably be enough mycelia to inoculate 200 quart jars.


Edited by eatyualive (07/19/15 07:55 PM)
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#21966924 - 07/19/15 07:50 PM (8 years, 6 months ago) |
|
|
Nvm found the link. Do you not worry about the interior of the grain being fully colonized?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#21966926 - 07/19/15 07:50 PM (8 years, 6 months ago) |
|
|
Quote:
maddchef said: Hey wait a fuggin mi the. You're slurrting colonized grain? Link me to said thread if it exists. Any slower or faster than LI and lc?
yes. did you watch the video in the op madchef?
ive been grain slurrying directly to bulk also.
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#21966932 - 07/19/15 07:51 PM (8 years, 6 months ago) |
|
|
So again what's the benefit over LI?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#21972130 - 07/20/15 06:23 PM (8 years, 6 months ago) |
|
|
i like both LI and slurry. i think the benefit would depend on the situation and timing.
for me i always have a leftover colonized quart or pint kept for transfers. that extra quart or pint will be g2ged or slurried for the next transfer. so it depends on the situation. but always having a colonized grain jar laying around is an advantage. why not use it?
if the need is speed. grain slurries are quick and easy. what i do is keep a master wbs pint jar reference in the fridge. ill keep all first transfer clones that are being slurried this way. there may be 6 clone masters ready for a 30 plus or more jar batch. at any time the jars can be used for immediate inoculant in less than a week to be spawning tubs.
let's say you are on your last leg of a nice clone, life circumstances caused you to run out of plates but you have a grain jar left. if you have no plates. but have a colonized grain jar. one spoonful can create many jars for spawn very quickly while using a small amount of inoculant. this may only be a timing thing you use when the time is right. for me, the time was right.
using one spoonful, is immediate and can create a very large volumes of spawn in a short period of time with little to work with. the grains blend up easier in my blender than a plate when you use a spoonful per pint of water..
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#21972403 - 07/20/15 07:13 PM (8 years, 6 months ago) |
|
|
So here's the situation: I use wide mouth quarts. I only have the freezer ball container thingy for LI now.
Are you saying I can dump some grain in there with water and puree the shit out of it instead of using a plate and it will work just as well if not better?
How much are you blending the grains and would it differ for me using oats? Also for say 8oz of liquid roughly how much grain would be added?
Are you limited to pouring the slurry or is it thin enough to use a syringe?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#21972408 - 07/20/15 07:14 PM (8 years, 6 months ago) |
|
|
Also how much of said slurry would you hit a 4qt bag with?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#21972435 - 07/20/15 07:23 PM (8 years, 6 months ago) |
|
|
Nvm man I actually took the time and read, duh.
Does splitting the grains open not cause bacterial issues though?
And if my only option is to pour grains in that container I'm wondering my water to grain ratio.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
Edited by maddchef (07/20/15 07:47 PM)
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#21973026 - 07/20/15 09:22 PM (8 years, 6 months ago) |
|
|
Quote:
maddchef said: So here's the situation: I use wide mouth quarts. I only have the freezer ball container thingy for LI now.
Are you saying I can dump some grain in there with water and puree the shit out of it instead of using a plate and it will work just as well if not better?
How much are you blending the grains and would it differ for me using oats? Also for say 8oz of liquid roughly how much grain would be added?
Are you limited to pouring the slurry or is it thin enough to use a syringe?
did you watch the video in the op? i blended for 3-5 seconds on the last one i did. carc, how long did you blend your grain?
madchef, my times with the GLC type slurry are about the same speed as LI. i haven't been able to get the grain slurries to colonize as fast as the pf jar slurries. it is essentially a GLC minus sucking it into a syringe and injecting it. your simply pouring it into quart jars.
https://www.youtube.com/watch?feature=player_embedded&v=Cu3sepCvnsY#t=97
you may have clogging if you suck grains into the syringe. it may be possible if you allow the grain to settle in the jar. then attempt to extract the liquid in an area far away from grain particles.
i think you may be asking for trouble as the grains particles are larger than a plate blend. worth a try? i probably would not try it with a syringe. pouring for me is much easier than using a syringe.
Edited by eatyualive (07/20/15 09:35 PM)
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#21973081 - 07/20/15 09:33 PM (8 years, 6 months ago) |
|
|
I can't be bothered to spend 6 minutes of my time watching some silly video.
Lol no I didn't even see it until now. The slurry looks quite thick almost like a milkshake so I guess syringes are out of the question.
Since my jar is already made maybe I'll hang a magnet from the top of my hood and glue another on the lid as a holder lol. I can just let it hang while I pour.
Maybe I should just stick to LI eh? Haha
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#21973134 - 07/20/15 09:43 PM (8 years, 6 months ago) |
|
|
this is from the op link. ive used a 2:1 grain to water ratio in the past. i wanted to try much less grain on this last attempt. i used one spoonful to a pint of water. my usual ratio is like this. and my tribal containers are ready for battle. too bad i don't have any empty jars.
Quote:
SLURRY TYPE 2: GS2G Transfer(Grain Slurry to Grain jar transfer): wbs grain jar blended and poured into other grain jars. A ratio of 1 pint grain spawn to 1/2 pint of water was used for 50 quarts of colonized grain.
Do all of this as sterile as possible. Research on sterile technique is assumed prior to this.
1. Fill a pint jar half full of distilled water and place the blade assembly on it. Pressure cook for 20 mins at 15psi. Allow jar to cool. 2. Prepare your clean area(Shmuvbox, SAB, Flowhood) and your substrates and tubs. Setup for spawning. 3. Using a phonebook, break up the grain inside the jar. 4. In your SAB, Take the colonized pint of grain loosen the lid. Loosen the lid of the sterile water pint jar. 5. pour water from water pint into grain pint. 6. place blade assembly lid on grain pint and water pint jar. 7. Blend on your oster blender base. Hold the jar in place with your hand while blending. I blend about 30 seconds. 8. In your SAB, Pour the grain slurry into your quarts jars. You pour by eye about 1 tablespoon volume of slurry per Quart Grain Jar. 9. Wait 3-4 days until full colonization. Give it a day or two more and then your ready to spawn your tubs. You can shake your grain a day before spawning to test for any contamination.
Regular Mouth Lids
 
Quarts and Pints(Pints will each be used as masters to slurry 100 more quarts each. In the last picture you can see all the colonized pints and 1 quart that i slurried. all the colonized jars are on the last bottom picture. each one of those pints will be used as a master for more than 30 jars at a time)judging from experience, each pint can do at least 200 quart jars of grain. i easily did 30 tonight and had to throw out 2/3rds of the pint jar of grain leftover.
   
Edited by eatyualive (07/20/15 09:55 PM)
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#21977174 - 07/21/15 07:40 PM (8 years, 6 months ago) |
|
|
day 1 LI and GLC inoculated.

day 2. the LI is slightly ahead. but more inoculant was used per jar with the LI. also only 20 quarts were used with LI. that is expected. the jars on the right are a Syzygy clone. Jars on the left are an amazon clone.

GLC Amazon

LI Syzygy
Edited by eatyualive (07/21/15 08:05 PM)
|
cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#21977457 - 07/21/15 08:34 PM (8 years, 6 months ago) |
|
|
11 day from inoculation of spawns to fruiting the tub 

tub 2 had a very thin layer of substrate fruiting probably tomorrow

i also inoculated some grain with a pe6 gwlc one day before the slurry.. they'll be ready in a day or two
so slurry 5 days lc 12-13 days...
--------------------
|
azur
God of Fuck



Registered: 04/21/12
Posts: 28,103
Loc: Daid
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#21977477 - 07/21/15 08:37 PM (8 years, 6 months ago) |
|
|
Quote:
cArcace-x said: 11 day from inoculation of spawns to fruiting the tub 

tub 2 had a very thin layer of substrate fruiting probably tomorrow

i also inoculated some grain with a pe6 gwlc one day before the slurry.. they'll be ready in a day or two
so slurry 5 days lc 12-13 days...

|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#21977738 - 07/21/15 09:33 PM (8 years, 6 months ago) |
|
|

tearing it up!
fastest i had was 12 from inoculation to harvest with casing. that involved using the substrate as casing method and a thai cubensis that was extremely fast. plus room temps are 76-80 degrees.
|
cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#21978953 - 07/22/15 04:57 AM (8 years, 6 months ago) |
|
|
12 days from inoculation to harvest...  thats crazy man!!!
thats another objective!!
--------------------
|
hamloaf
Loaf of Fam.


Registered: 12/23/09
Posts: 20,192
Loc: Oklahoma.
Last seen: 1 year, 8 months
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#21978985 - 07/22/15 05:11 AM (8 years, 6 months ago) |
|
|
Temp range was just right for rapid colonization. The rest is genetic rigorousness indeed. Bet the fruits were long, lanky, hollow, and mean too.
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#21981997 - 07/22/15 08:53 PM (8 years, 6 months ago) |
|
|
Quote:
eatyualive said: day 1 LI and GLC inoculated.

day 2-3. the LI is slightly ahead. but more inoculant was used per jar with the LI. also only 20 quarts were used with LI. that is expected. the jars on the right are a Syzygy clone. Jars on the left are an amazon clone.
 
GLC Amazon
 
LI Syzygy

|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#21986487 - 07/23/15 06:06 PM (8 years, 6 months ago) |
|
|
so my craftsmanship is horrible but you get the idea. have only seen the wide mouth version of this lid. today they had the regular mouth.
found these regular mouth lids for sip cups. you put the injection port in. wrap it in foil. pressure cook with the regular half pint, pint or quart with blade assembly on them. then after transfer and blend. switch to this lid for pouring. easy and it fits perfectly into the ring. so it was siliconed in.
   
day 1 LI and GLC inoculated.

day 2-4. the LI is slightly ahead. but more inoculant was used per jar with the LI. also only 20 quarts were used with LI. that is expected. the jars on the right are a Syzygy clone. Jars on the left are an amazon clone.
  
GLC Amazon
  
LI Syzygy
 
Edited by eatyualive (07/23/15 09:46 PM)
|
cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#21988760 - 07/24/15 07:38 AM (8 years, 6 months ago) |
|
|
Quote:
eatyualive said: found these regular mouth lids for sip cups. you put the injection port in. wrap it in foil. pressure cook with the regular half pint, pint or quart with blade assembly on them. then after transfer and blend. switch to this lid for pouring. easy and it fits perfectly into the ring. so it was siliconed in.
thats how i used to do before i made the container
--------------------
|
cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#22006726 - 07/27/15 08:27 PM (8 years, 6 months ago) |
|
|
tub #1 is knotting up #2 had a thin late casing so i dunno whats happen in there but i'm sure it will follow by 2-3 days behind
 
also i found the first plug i made to pour LI  handcrafted with RTV and a bolt 
  
i was sterilizing this lid in foil beside the jar with water and the blade on, blend and change for the lid in the sab and then pour.... was a pain in the ass ahaha
--------------------
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#22006961 - 07/27/15 09:21 PM (8 years, 6 months ago) |
|
|
Hey eat when I made mine with the snap freezer jar it concaved the top when it cooled. What gives?
I used the stock lid and merely made an LI jar and put a ship in the lid. You had issues like that or how can I avoid it?
I'm assuming it's because it makes such a good seal that it acted like a canning jar, does your build not do that?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#22007018 - 07/27/15 09:33 PM (8 years, 6 months ago) |
|
|
did you put a syringe filter or something on it? it may help to release the pressure while PC and cooling
--------------------
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#22007158 - 07/27/15 10:10 PM (8 years, 6 months ago) |
|
|
Did not. I didn't figure it made a good enough seal to cause the issue and I didn't see one on yours?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#22007174 - 07/27/15 10:14 PM (8 years, 6 months ago) |
|
|
no but the plug for pouring is slack a bit so it probably release by there... and you have only a tight SHIP i will specify this in the OP
--------------------
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#22007183 - 07/27/15 10:17 PM (8 years, 6 months ago) |
|
|
I figured your plug was tight.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
Alkaloids
3,4,5-trimethoxyphenethylamine



Registered: 11/15/98
Posts: 743
Loc: pubis mons
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef] 1
#22007634 - 07/28/15 12:29 AM (8 years, 6 months ago) |
|
|
Great DIY and how to thread. Props to all the contributors on this.
|
Purplecapz
The Wandering Psilosopher



Registered: 06/16/13
Posts: 60
Last seen: 7 years, 11 months
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: Alkaloids] 1
#22007664 - 07/28/15 12:44 AM (8 years, 6 months ago) |
|
|
legit
|
cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: Purplecapz]
#22008587 - 07/28/15 09:22 AM (8 years, 6 months ago) |
|
|
 cheers!
--------------------
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22012326 - 07/28/15 09:45 PM (8 years, 6 months ago) |
|
|
Quote:
cArcace-x said: tub #1 is knotting up #2 had a thin late casing so i dunno whats happen in there but i'm sure it will follow by 2-3 days behind
 
also i found the first plug i made to pour LI  handcrafted with RTV and a bolt 
  
i was sterilizing this lid in foil beside the jar with water and the blade on, blend and change for the lid in the sab and then pour.... was a pain in the ass ahaha
awesome carc! can't wait. thanks for posting that build! i used this build for some Penis Envy LI. uh oh....i got leap off the next day! genetics came from the Penis Master!


so my GLC and LI jars are almost finished. The LI jars won slightly. I had to shake both because they were taking forever(more than 4 days). Im spoiled by slurry. and the higher grain volume grain slurries. but for pure mycelial water, the colonization was just as fast as the LI from a grain jar. 9 days full colonization. even slow on my end for g2g. this last session I went real heavy on the LI with a pint of water to 1 plate. Poured 26 quarts of PE. had about 5 more jars worth of LI leftover. so this higher volume of water on the inoculation of LI actually showed leap off faster. I think the higher volume of water doesn't chop up the agar plate as much. There were much larger chunks than I normally have in the blender jar. I also pulsed it twice for about 3-5 seconds.
Grain slurry and slurry are take half the time as LI to colonize 100% without a shake. although ive compared them several times. i will still continue to do so in this thread. The LI and GLC took 9 days total with a shake.
Quote:
eatyualive said:
day 1-9: only 20 quarts were used with LI. 30 used with the GLC. the jars on the right of the colonized quart are a Syzygy clone. Jars on the left are an amazon clone.
     day 7 after a shake on day 5
 
GLC Amazon
    
LI Syzygy
    
Edited by eatyualive (07/29/15 07:35 AM)
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22012341 - 07/28/15 09:47 PM (8 years, 6 months ago) |
|
|
Did you just say grain slurry is half the speed of LI?
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#22013612 - 07/29/15 07:33 AM (8 years, 6 months ago) |
|
|
I updated the the op. No a 2:1 grain to water slurry is about twice as fast. The lower volume 1 spoonfull of grain to water GLC is the same speed as the LI. By half speed i meant took half as much time to colonize 100%. Ill update that line of text for clarity. It was stated incorrectly. This involved inoculating the jars with the mycelial water only. No grains were poured into receiving jars. Whereas, with the grain slurry you pour in the water and the blended grain into receiving jars.
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22014188 - 07/29/15 10:15 AM (8 years, 6 months ago) |
|
|
I'll try some grain slurry on my next batch and see if I fare better.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
kiksen


Registered: 06/04/15
Posts: 60
Last seen: 2 months, 25 days
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#22014466 - 07/29/15 11:21 AM (8 years, 6 months ago) |
|
|
Awesome work!
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: kiksen]
#22021897 - 07/30/15 08:29 PM (8 years, 5 months ago) |
|
|
Day 4 of the higher water volume LI with PE.
|
Syntheticwords
Victus


Registered: 08/13/11
Posts: 375
Last seen: 6 years, 9 months
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22030449 - 08/01/15 08:17 PM (8 years, 5 months ago) |
|
|
Anyone think about using this for a container?
Maybe it won't survive in a pc

Strictly to use agar or perhaps grain slurry? No nocing up
Edited by Syntheticwords (08/01/15 08:18 PM)
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: Syntheticwords]
#22030454 - 08/01/15 08:18 PM (8 years, 5 months ago) |
|
|
Can't use that, its in Spanish.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
Syntheticwords
Victus


Registered: 08/13/11
Posts: 375
Last seen: 6 years, 9 months
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#22030460 - 08/01/15 08:20 PM (8 years, 5 months ago) |
|
|
Quote:
maddchef said: Can't use that, its in Spanish.
|
Darkhome
•Shaman•N•Training•



Registered: 07/10/15
Posts: 517
Loc: United States
Last seen: 7 years, 1 month
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: Syntheticwords]
#22030466 - 08/01/15 08:22 PM (8 years, 5 months ago) |
|
|
No bueno?
-------------------- “The shaman is not merely a sick man, or a madman; he is a sick man who has healed himself.” ~Terence McKenna~ "NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.
|
Syntheticwords
Victus


Registered: 08/13/11
Posts: 375
Last seen: 6 years, 9 months
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: Darkhome]
#22030471 - 08/01/15 08:24 PM (8 years, 5 months ago) |
|
|
I will report of this melts in the pressure cooker or not...hopefully it won't explode or something
No English versions
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: Syntheticwords]
#22031347 - 08/01/15 11:43 PM (8 years, 5 months ago) |
|
|
if the base is plastic it may melt. can you take a picture of the blade attachment to the base of the blender glass? neat thing about the oster blenders is that they fit on regular mouth mason jars really easy. this one looks very wide. im also not sure if it has a opour spout on top.
you won't want to put the plastic in the pc unless is pp5.
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22040031 - 08/03/15 10:08 PM (8 years, 5 months ago) |
|
|
Here are some of the GLC slurry jars before spawning. Also the Syzygy clone 2 LI was spawned to 4 tubs!
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive] 1
#22113312 - 08/19/15 05:58 PM (8 years, 5 months ago) |
|
|
So here is an update. I did a side by side with slurry and LI.
The Liquid inoculant was inoculated on 8-11-2015. The slurry was inoculated on 8-16-2015. The Liquid inoculant is a fresh clone Texas Yellow Cap plate from agar. The slurry is a 1 years old multispore Thai Lipa Yai from a pf jar that had been sitting in the fridge from 2014.
As you can see the first image was taken on 8-18-2015. 2 days after the slurry was inoculated. The last picture was taken today. So the LI took roughly 8 days to colonize 99%. The slurry in 3 days has almost caught up with the LI. Neither set of jars has been shaken since inoculation.
If you zoom in you can see the tape label. The LI jars are to the left of the LI label. The Slurry jars are to the right of the label. So half the speed on grain. This is consistent for both LI and Slurry with a multitude of tests.
 
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22117931 - 08/20/15 06:10 PM (8 years, 5 months ago) |
|
|
So here is an update. I did a side by side with slurry and LI.
The Liquid inoculant was inoculated on 8-11-2015. The slurry was inoculated on 8-16-2015. The Liquid inoculant is a fresh clone Texas Yellow Cap plate from agar. The slurry is a 1 years old multispore Thai Lipa Yai from a pf jar that had been sitting in the fridge from 2014.
As you can see the first image was taken on 8-18-2015. 2 days after the slurry was inoculated. The last picture was taken today. So the LI took roughly 8 days to colonize 99%. The slurry in 3 days has almost caught up with the LI. Neither set of jars has been shaken since inoculation.
If you zoom in you can see the tape label. The LI(texan yellow cap clone-3rd transfer plate) jars are to the left of the LI label. The Slurry jars(Thai Lipa Yai multispore pf jar) are to the right of the label. So half the speed on grain. This is consistent for both LI and Slurry with a multitude of tests.
a little background on these cultures. the thai culture is the fastest growing cube culture i have. tex is 2nd fastest. very close in speed. but the thai is multispore and the tex is a clone. the thai pf jar sat in the fridge for over a year and still caught up to the fresh tex clone culture.
fun. check it out. day 4 on the slurry almost done. caught right up to the LI that had a 6 day head start.
  
|
invitro

Registered: 05/03/13
Posts: 2,529
Last seen: 1 month, 20 days
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#22117978 - 08/20/15 06:19 PM (8 years, 5 months ago) |
|
|
|
taGyo
Strainiac/AMU



Registered: 10/16/14
Posts: 18,802
Loc: Journal Land
Last seen: 5 years, 3 months
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22118013 - 08/20/15 06:28 PM (8 years, 5 months ago) |
|
|
-------------------- Gyo's Better Grows TNF Q&A AMU Q&A Dominus fortunae meae sum
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: taGyo]
#22118180 - 08/20/15 07:11 PM (8 years, 5 months ago) |
|
|
The slurry I did did OK. I would got close to the same results from regular g2g. Split a pint of grain between 10 bags
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#22118246 - 08/20/15 07:31 PM (8 years, 5 months ago) |
|
|
furious eatyualive!
update of the grow... one of the tub got caugh trich.. I removed it and recovered well

the other one went fine, had 2 flush, the second only few fruits... never rehydrated it because I was too buzy and didn't had enough fuck to give 1st flush

and some fruits of the second flush a bit bigger 
--------------------
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#22118296 - 08/20/15 07:46 PM (8 years, 5 months ago) |
|
|
  badass carc.

Quote:
maddchef said: The slurry I did did OK. I would got close to the same results from regular g2g. Split a pint of grain between 10 bags
grain slurry? how long did it take and what size bags of grain were you using?
yeah its slower than a pf jar slurry. its more on the speed of a liquid inoculant with a plate and GLC. but you can use that same pint of grain to inoculate at least 100 quarts! thats the beauty of it. where if i g2ged that pint id get maybe 10 that would take longer than a week to colonize with a shake.
Edited by eatyualive (08/20/15 07:53 PM)
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22123345 - 08/21/15 04:30 PM (8 years, 5 months ago) |
|
|
final update.
The Liquid inoculant(texan yellow cap clone-3rd transfer plate) was inoculated on 8-11-2015. The slurry(Thai Lipa Yai multispore pf jar) was inoculated on 8-16-2015. The Liquid inoculant is a fresh clone Texas Yellow Cap plate from agar. The slurry is a 1 years old multispore Thai Lipa Yai from a pf jar that had been sitting in the fridge from 2014.
As you can see the first image was taken on 8-18-2015. 2 days after the slurry was inoculated. The last picture was taken today. So the LI took roughly 8 days to colonize 99%. The slurry in 3 days has almost caught up with the LI. Neither set of jars has been shaken since inoculation.
If you zoom in you can see the tape label. The LI jars are to the left of the LI label. The Slurry jars are to the right of the label. So half the speed on grain. This is consistent for both LI and Slurry with a multitude of tests.
A little background on these cultures. the thai culture is the fastest growing cube culture i have. tex is 2nd fastest. very close in speed. but the thai is multispore and the tex is a clone. the thai pf jar sat in the fridge for over a year and still caught up to the fresh tex clone culture.
check it out. day 4 on the slurry almost done. caught right up to the LI that had a 5 day head start.
    
Here are some of the LI and Slurry jars that were in the back pulled to the front. As you can see, the LI jars are not 100%. Most of them are. But there are a few that are not. The slurry jars on the other hand, are all 99% or finished.
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22123867 - 08/21/15 06:34 PM (8 years, 5 months ago) |
|
|
Yeah it was grain slurry to 4qt bags. I believe I made it too thick for my liking though so technically it did 40qts since it was split between 10 bags.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#22124010 - 08/21/15 07:18 PM (8 years, 5 months ago) |
|
|
ok. Yeah maybe you needed to mix it up a little more. i usually shake each jar for about 20-30 seconds. so if its a big bag like that, you may need to move it around to get the slurry everywhere a little more.
|
maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
Loc: Your mom's vag
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22125199 - 08/22/15 12:20 AM (8 years, 5 months ago) |
|
|
I may do it again sometime soon but for now imma stop fucking with blenders, pouring, mixing, chopping, slicing, dicing etc.
Gonna dive back into lc's for a little while but let it be known that your numbers hold up.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
|
The Dom
Stranger
Registered: 08/28/14
Posts: 30
Last seen: 4 years, 1 month
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22514698 - 11/12/15 04:02 PM (8 years, 2 months ago) |
|
|
Quote:
eatyualive said: look what i found. I think this is the one Dhearic found. I will give it a try. It's huge she said.
 
the only downside i see is height of SAB. Also when you get to the end it might be difficult flipping it over 180%. But you can fit twice the volume of LI into it. The lid is somewhat loose. Enough that its easy to push down and open. However, this might be an issue when pressure cooking. I will likely use foil over the lid or micropore tape to hold the lid in place while pressure cooking.
This item was 8% from walmart. The plastic is sturdy.
Sorry for bringing this back but i thought it would be good to have clarification in-thread. We dont need to add anything to the lid for the blender bottle except SHIP(if we want) and syringe filter(if we want) because it already has the pour spout? and was the clear you used 100% silicone? Ive got some now and I'd rather make another trip to the store if what I have now wont hold up
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: The Dom]
#22514841 - 11/12/15 04:47 PM (8 years, 2 months ago) |
|
|
if you only intend to blend and pour. then no for that blender bottle. . you would attach the bottom blade assembly and seal that to the blender jar. if you wanted to store the liquid inoculant, then it might be beneficial to add a ship.
i place a piece of foil over the lid to keep it sealed during pressure cook cycles. i only use it to blend and immediately pour.
it makes a great amount of liquid inoculant for use.
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22514950 - 11/12/15 05:18 PM (8 years, 2 months ago) |
|
|
a few tubs done with cornmeal plate liquid inoculation using the tribal container!
   
         
 
Edited by eatyualive (12/09/15 08:59 PM)
|
The Dom
Stranger
Registered: 08/28/14
Posts: 30
Last seen: 4 years, 1 month
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22517492 - 11/13/15 10:22 AM (8 years, 2 months ago) |
|
|
nice pics, ive been reading through a lot of your posts lately eatyualive, good stuff! thanks for the clarification
|
The Dom
Stranger
Registered: 08/28/14
Posts: 30
Last seen: 4 years, 1 month
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: The Dom]
#22522249 - 11/14/15 12:38 PM (8 years, 2 months ago) |
|
|
so wait are your blender bottle tops PP5 also? my base is but my lid is HDPE, this will melt in pc wont it?
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: The Dom]
#22522297 - 11/14/15 12:46 PM (8 years, 2 months ago) |
|
|
yes you want pp5. im not sure about the hdpe. but i wouldn't risk it.
|
The Dom
Stranger
Registered: 08/28/14
Posts: 30
Last seen: 4 years, 1 month
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: Dhearic]
#22526020 - 11/15/15 01:29 AM (8 years, 2 months ago) |
|
|
Quote:
Dhearic said:


Doesn't leak and blends myc like a charm 
Would either you or eatyualive mind checking if your lid is made of something else? After posting I found somewhere that the melting pt varies but is around 260f so it seems possible it may survive pc cycle, if i dont get a definitive answer back I'll pc it in an autoclave bag so I dont make a mess. The rest of my container is pp5 btw its the same kind as the one in the pic just a diff color.
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: The Dom]
#22526041 - 11/15/15 01:44 AM (8 years, 2 months ago) |
|
|
my black lid has a 2 on it. and ive pced it many times for 20 minutes at 15psi.
|
cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22526526 - 11/15/15 07:51 AM (8 years, 2 months ago) |
|
|
might be usefull 
Quote:
POLYPROPYLENE (PP)
Translucent Autoclavable Maximum temperature: 135°C (275°F) Brittleness temperature: 0°C (32°F)
Polypropylene has relatively poor impact strength, but excellent compatibility with weak and concentrated acids, bases and alcohol.* Polypropylene is commonly used to make bottles, vials, pumps, funnels and clothing.
POLYETHYLENE (PE)
Translucent Not autoclavable Maximum temperature for high-density polyethylene (HDPE) is 120°C (248°F) Maximum temperature for low-density polyethylene (LDPE) is 80°C (176°F) Brittleness temperature for both HDPE and LDPE is -100°C (-100°F)
Polyethylene has high impact strength and excellent compatibility with weak and concentrated acids, bases and alcohol.* Polyethylene is used to manufacture waste bags, bottles, refuse containers, pumps and secondary containment platforms.
POLYCARBONATE (PC)
Transparent Autoclavable Maximum temperature: 135°C (275°F) Brittleness temperature: -135°C (-135°F)
Polycarbonate has high impact strength and excellent compatibility with weak acids.* Polycarbonate is commonly used to make non-breakable windows, safety eyewear and face shields.
POLYMETHYLPENTENE (PMP)
Transparent Autoclavable Maximum temperature: 175°C (347°F) Brittleness temperature: 20°C (68°F)
Polymethylpentene has excellent compatibility with weak and concentrated acids, bases and alcohol.* Polymethylpentene is commonly used to make beakers and graduated cylinders.
PTFE Resin
Opaque Autoclavable Maximum temperature: 260°C (50°F) Brittleness temperature: -100°C (-100°F)
PTFE resin has excellent compatibility with almost all chemicals.* PTFE resin is used to produce pumps, stoppers, tubing, containers, non-stick pans and samplers.
POLYVINYL CHLORIDE (PVC or Vinyl)
Clear Not autoclavable Maximum temperature: 70°C (158°F) Brittleness temperature: -30°C (-22°F)
Polyvinyl chloride has excellent compatibility with almost all chemicals.* Polyvinyl chloride is used to manufacture plumbing pipes, raincoats, footwear, earplugs, pumps and tubing.
POLYETHYLENE TEREPHTHALATE COPOLYMER (PET)
Clear Not autoclavable Maximum temperature: 70°C (158°F) Brittleness temperature: -40°C (-40°F)
Polyethylene terephthalate copolymer has excellent compatibility with weak acids, alcohol and aliphatic hydrocarbons.* It is used to make face shields and bottles.
POLYSTYRENE (PS)
Clear Not autoclavable Maximum temperature: 90°C (194°F) Brittleness temperature: -20°C (-4°F)
Polystyrene has high strength and impact resistance and excellent compatibility with weak acids, alcohol and bases.* Ice buckets and scoops are commonly made with polystyrene.
POLYMETHYL METHACRYLATE (PMMA or Acrylic)
Clear Autoclavable Maximum temperature: 50°C (122°F) Brittleness temperature: 20°C (68°F)
Polymethyl methacrylate does not have excellent compatibility with any group of chemicals.* Bench top shields and machine guards are made from polymethyl methacrylate.
*Polymers described as having excellent compatibility are generally capable of withstanding damage from the chemicals named for 30 days of constant exposure. This is only a general indication. Always check chemical compatibility with each chemical for the containers used for them.
The following plastics chart supplied by Nalgene, includes all of the plastics mentioned in this document. This chart gives many useful ratings pertaining to these plastics including temperature tolerances, permeability and sterilization processes.
http://www.grainger.com/content/qt-types-of-plastics-213
--------------------
|
The Dom
Stranger
Registered: 08/28/14
Posts: 30
Last seen: 4 years, 1 month
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#22536285 - 11/17/15 07:29 AM (8 years, 2 months ago) |
|
|
Quote:
eatyualive said: my black lid has a 2 on it. and ive pced it many times for 20 minutes at 15psi.
Thanks for confirming I was not looking forward to the trial run
Quote:
cArcace-x said: might be usefull 
Quote:
POLYPROPYLENE (PP)
Translucent Autoclavable Maximum temperature: 135°C (275°F) Brittleness temperature: 0°C (32°F)
Polypropylene has relatively poor impact strength, but excellent compatibility with weak and concentrated acids, bases and alcohol.* Polypropylene is commonly used to make bottles, vials, pumps, funnels and clothing.
POLYETHYLENE (PE)
Translucent Not autoclavable Maximum temperature for high-density polyethylene (HDPE) is 120°C (248°F) Maximum temperature for low-density polyethylene (LDPE) is 80°C (176°F) Brittleness temperature for both HDPE and LDPE is -100°C (-100°F)
Polyethylene has high impact strength and excellent compatibility with weak and concentrated acids, bases and alcohol.* Polyethylene is used to manufacture waste bags, bottles, refuse containers, pumps and secondary containment platforms.
POLYCARBONATE (PC)
Transparent Autoclavable Maximum temperature: 135°C (275°F) Brittleness temperature: -135°C (-135°F)
Polycarbonate has high impact strength and excellent compatibility with weak acids.* Polycarbonate is commonly used to make non-breakable windows, safety eyewear and face shields.
POLYMETHYLPENTENE (PMP)
Transparent Autoclavable Maximum temperature: 175°C (347°F) Brittleness temperature: 20°C (68°F)
Polymethylpentene has excellent compatibility with weak and concentrated acids, bases and alcohol.* Polymethylpentene is commonly used to make beakers and graduated cylinders.
PTFE Resin
Opaque Autoclavable Maximum temperature: 260°C (50°F) Brittleness temperature: -100°C (-100°F)
PTFE resin has excellent compatibility with almost all chemicals.* PTFE resin is used to produce pumps, stoppers, tubing, containers, non-stick pans and samplers.
POLYVINYL CHLORIDE (PVC or Vinyl)
Clear Not autoclavable Maximum temperature: 70°C (158°F) Brittleness temperature: -30°C (-22°F)
Polyvinyl chloride has excellent compatibility with almost all chemicals.* Polyvinyl chloride is used to manufacture plumbing pipes, raincoats, footwear, earplugs, pumps and tubing.
POLYETHYLENE TEREPHTHALATE COPOLYMER (PET)
Clear Not autoclavable Maximum temperature: 70°C (158°F) Brittleness temperature: -40°C (-40°F)
Polyethylene terephthalate copolymer has excellent compatibility with weak acids, alcohol and aliphatic hydrocarbons.* It is used to make face shields and bottles.
POLYSTYRENE (PS)
Clear Not autoclavable Maximum temperature: 90°C (194°F) Brittleness temperature: -20°C (-4°F)
Polystyrene has high strength and impact resistance and excellent compatibility with weak acids, alcohol and bases.* Ice buckets and scoops are commonly made with polystyrene.
POLYMETHYL METHACRYLATE (PMMA or Acrylic)
Clear Autoclavable Maximum temperature: 50°C (122°F) Brittleness temperature: 20°C (68°F)
Polymethyl methacrylate does not have excellent compatibility with any group of chemicals.* Bench top shields and machine guards are made from polymethyl methacrylate.
*Polymers described as having excellent compatibility are generally capable of withstanding damage from the chemicals named for 30 days of constant exposure. This is only a general indication. Always check chemical compatibility with each chemical for the containers used for them.
The following plastics chart supplied by Nalgene, includes all of the plastics mentioned in this document. This chart gives many useful ratings pertaining to these plastics including temperature tolerances, permeability and sterilization processes.
http://www.grainger.com/content/qt-types-of-plastics-213
Thank you but I found those and other similar temp ratings for hdpe suggesting it would not survive the pc but firsthand experience from eatyualive was exactly what I needed
|
cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: The Dom]
#22538834 - 11/17/15 07:08 PM (8 years, 2 months ago) |
|
|
cool, i was just posting beceause it can be usefull
post result if you want
--------------------
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#22578046 - 11/26/15 12:04 PM (8 years, 2 months ago) |
|
|
ive finally updated my tribal lid to have the magnets.
|
cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22603033 - 12/02/15 07:18 AM (8 years, 1 month ago) |
|
|
i'm waiting for diamond bits............
--------------------
|
Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#22709048 - 12/28/15 12:54 PM (8 years, 1 month ago) |
|
|
Anyone use the oster milkshake blender blades to make their LI?
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: Juiceh]
#22709212 - 12/28/15 01:43 PM (8 years, 1 month ago) |
|
|
i haven't seen those. do you know if they are pp5?
|
Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22709620 - 12/28/15 04:00 PM (8 years, 1 month ago) |
|
|
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: Juiceh]
#22709637 - 12/28/15 04:04 PM (8 years, 1 month ago) |
|
|
hmm. it may agitate the mycelia off of a plate or wedge. but, it may not break the wedge into pieces. it would be more like a GLC than a LI. i do think it will work. but the recovery may be slower if you don't have the chunks of the agar/mycelia. id give it a try though.
|
tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 7 months, 30 days
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22973603 - 03/04/16 05:05 PM (7 years, 10 months ago) |
|
|
Edited by tombosley8 (03/04/16 05:16 PM)
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: tombosley8]
#22973820 - 03/04/16 06:06 PM (7 years, 10 months ago) |
|
|
it is the bigger 28 oz size. but i think someone in this thread built the smaller one also.
i like the big one. but have not tried the smaller one. it really depends on how much inoculant you need. even if you don't need the bigger unit, you can still have the option of being able to use more inoculant if needed. but if you only have 20 jars. the smaller bottle should work.
|
Mycophreek423
Late For TheFuture!!



Registered: 01/07/06
Posts: 109
Loc: The Tenth Kingdom
Last seen: 4 years, 4 months
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#23279335 - 05/27/16 11:20 PM (7 years, 7 months ago) |
|
|
if you use silicone is great idea to wipe area with iso before applying silicone, it helps remove oils.
-------------------- I Thought You Where There , But you Where not. It Mushed Have Been Somthing I Ate.
|
Pete Moss
Stranger
Registered: 08/17/16
Posts: 1
Last seen: 7 years, 5 months
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: Mycophreek423]
#23549529 - 08/17/16 10:34 AM (7 years, 5 months ago) |
|
|
Has anyone gone full bore and attempted to make a blender assembly lid with some sort of injection site for use on a quart jar with a small pf cake amount of pf mix/grains on the bottom from the get go? Allowing a person to pc the whole shebang, inoculate and blend after colonization without even opening the jar?
PC a dozen 'jars' and have enough inoculum when they colonize to knock what, 420 some odd quarts? Madness.
|
Chips
Stranger

Registered: 01/05/16
Posts: 32
Last seen: 5 years, 2 months
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#23873189 - 11/27/16 07:35 PM (7 years, 2 months ago) |
|
|
Quote:
eatyualive said:
Taller Higher Volume Container Build
the only downside i see is height of SAB. Also when you get to the end it might be difficult flipping it over 180%. But you can fit twice the volume of LI into it. The lid is somewhat loose. Enough that its easy to push down and open. However, this might be an issue when pressure cooking. I will likely use foil over the lid or micropore tape to hold the lid in place while pressure cooking.
This item was 8$ from walmart. The plastic is sturdy.
 
1/4" guide hole drilled.

3" hole saw drilled.
trimmed around the edge with an exacto.

sanded down with sand paper. blade assembly fits perfect. 
rtv 8" container

testing for any leaks tonight.

tested no leaks

building my taller 28oz blender bottle tonight, just wanted to mention the hole saw should be 2" to make the proper hole, as 3" is wider than the whole base of the bottle:

|
wtfcrazymofo
foil hater



Registered: 07/26/15
Posts: 1,201
Loc: Colonial alley
Last seen: 2 hours, 45 seconds
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#23891846 - 12/03/16 07:53 PM (7 years, 1 month ago) |
|
|
-------------------- If you want to eat->https://www.shroomery.org/forums/showflat.php/Number/8553541 Bag sealers are to bulky (my hood isn't that big) https://www.shroomery.org/forums/showflat.php/Number/28622922
|
tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: wtfcrazymofo]
#23905719 - 12/08/16 02:54 AM (7 years, 1 month ago) |
|
|
Better question is there a way to cut the bottom of the blender bottle out without a drill bit. With any knife ?
Edited by tump (12/08/16 09:20 PM)
|
tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: tump]
#23917056 - 12/11/16 05:15 PM (7 years, 1 month ago) |
|
|
Knife and candle for the win 37 seconds.
|
Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: tump]
#23919095 - 12/12/16 10:32 AM (7 years, 1 month ago) |
|
|
Quote:
tump said: Knife and candle for the win 37 seconds.
Looks like you didn't leave anywhere near enough flat plastic on the bottom of the bottle to mount the blender assembly to. Months back I drilled mine out with a 2 1/4" hole saw. But it didn't leave enough flat plastic surface to get a decent silicone bead on the base of the bottle. I wound up with leaks. I need to try again sometime with a 2" bit. Using a qt mason jar with a blender lid and the mason jar pour lids has worked out great with 0 issues. So I haven't bothered revisiting the blender bottle after wrecking 2.
If you are going to try again with the candle and knife made sure you leave a lot more of the flat bottom to mount the blender assembly to. It looks like with all those jagged edges from the hot knife you are gonna have a hard time getting a really good seal on the base without trimming and sanding that down real well. Good luck!
|
tombosley8
Full on... Bossley Baggins



Registered: 10/14/13
Posts: 3,660
Last seen: 7 months, 30 days
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: Juiceh]
#23920483 - 12/12/16 06:01 PM (7 years, 1 month ago) |
|
|
Yeah I had some problems with leaks for similar reasons I believe and ended having to use a ton of glue after sanding the surface and glueing the whole base and up the sides of the bottle quite far.
I think the more of the platic you can leave the better.
That one may still work tump with the techniques I used and maybe a second coat to harden the silicon a bit for durability and to ensure the seal.
I found the thicker that glue is the harder it forms and makes it much more durable.
--------------------
|
tump
ban the undead



Registered: 03/17/16
Posts: 2,383
Last seen: 6 years, 10 months
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: tombosley8]
#23920639 - 12/12/16 06:42 PM (7 years, 1 month ago) |
|
|
Thank you ill try a second thinker coat
|
synthetik
biological entity

Registered: 12/15/16
Posts: 194
Last seen: 3 years, 10 months
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: tump]
#24182320 - 03/22/17 04:52 AM (6 years, 10 months ago) |
|
|
sorry to rehash an older thread, doing a little late night research while I wait on my trusty AA to finish so I can sleep...
I see why you guys banned tump, what a goddamn moron not just this thread... it almost seems like hes trying to be funny? I dunno just had to throw that out there... fucking "knife and candle for the win"... no dude, just stop... stop everything...
|
BreakTheSOB
Stranger



Registered: 07/09/17
Posts: 162
Last seen: 6 years, 2 months
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#24511105 - 07/27/17 05:51 AM (6 years, 6 months ago) |
|
|
Are the sip and straw lids regular or wide mouth? Was about to order some.
-------------------- "A common sense interpretation of the facts suggests that a super intellect has monkeyed with physics, as well as with chemistry and biology, and that there are no blind forces worth speaking about in nature." Physicist Fred Hoyle
|
eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: BreakTheSOB]
#24512621 - 07/27/17 07:24 PM (6 years, 6 months ago) |
|
|
Regular they may make them for wide mouth but I've never seen them in stores.
|
TDog
Buddy


Registered: 06/23/18
Posts: 227
Loc: Corner of Laydown and Sta...
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#25295866 - 06/27/18 08:49 PM (5 years, 6 months ago) |
|
|
I don't know when they started making these, but I'm sure everyone has moved towards using something like this already...
Autoclavable Plastic (PP/Polypropylene) screw top jars/bottles (quart size).
https://www.amazon.com/Aibelong-Autoclavable-Transparent-Plastic-Laboratory/dp/B074DT8LMD/ref=sr_1_1?ie=UTF8&qid=1530153585&sr=8-1&keywords=autoclave+plastic+bottle
I'm looking forward to this VERY much.
(Much thanks to tvcasualty and eatyualive for all of this stuff in general. #slurryinahurry and slurry to sub tests [you guys KILL IT] <<< !!!)
-------------------- "Laugh, and the world laughs with you. Cry, and they will kick you in the fuc**ng teeth." -TDog
|
boom boom

Registered: 02/27/18
Posts: 429
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: TDog]
#25296014 - 06/27/18 10:38 PM (5 years, 6 months ago) |
|
|
Quote:
TDog said: I don't know when they started making these, but I'm sure everyone has moved towards using something like this already...
Autoclavable Plastic (PP/Polypropylene) screw top jars/bottles (quart size).
https://www.amazon.com/Aibelong-Autoclavable-Transparent-Plastic-Laboratory/dp/B074DT8LMD/ref=sr_1_1?ie=UTF8&qid=1530153585&sr=8-1&keywords=autoclave+plastic+bottle
I'm looking forward to this VERY much.
(Much thanks to tvcasualty and eatyualive for all of this stuff in general. #slurryinahurry and slurry to sub tests [you guys KILL IT] <<< !!!)
Those look awfully expensive. You can 12 of the glass ones for $10.
|
TDog
Buddy


Registered: 06/23/18
Posts: 227
Loc: Corner of Laydown and Sta...
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: boom boom]
#25296049 - 06/27/18 11:03 PM (5 years, 6 months ago) |
|
|
Yes.
I like that the glass ones go for about $1 each. I also have 60 of them. Lol.
I was looking for something that is squeezable, and that I would not have to transfer to/from. Just autoclave, inoculate, and then go straight to the blender base to slurry from and squeeze it in to other jars or go straight to the substrate with it.
I contacted the community/seller and those can only stand up to 121c/250f for 20 minutes. So, back to the drawing board.
(I'm thinking that I will have to custom make what I need. As in I will have to mold them and bulk process pour liquid, semi-rigid, high-temp silicone.)
It bothers me that there is not an easily adaptable vessel that can do it all.
The original post that tvcasualty put up on the other forum states that he was thinking along that type of line... a "one jar to rule them all" type container.
Autoclavable. Squeezeable. Patch Hole. Blender assembly. Injection site. Transfer (inlet) site.
The vessel to end all vessels.
Lol.
-------------------- "Laugh, and the world laughs with you. Cry, and they will kick you in the fuc**ng teeth." -TDog
|
TDog
Buddy


Registered: 06/23/18
Posts: 227
Loc: Corner of Laydown and Sta...
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: TDog]
#25296060 - 06/27/18 11:17 PM (5 years, 6 months ago) |
|
|
(If anyone knows of something like that I'm all ears.
I'm researching day in and day out.
It's possible there is a 3D printer filament out there that would work as well. I'm not sure.
I would even take suggestions of what type of base material would work if it's mold poured, etc.
Still looking. Daily.
I'm hoping that some obscure vessel exists already. Maybe on some scientific website that is buried in the web.
That one I linked would be perfect if it could stand up to the heat(/time). It probably has something to do with the softeners in the polypropylene they used, but I really do not know.
Still looking.
Hopefully Frodo didn't throw it in the volcano yet...)
-------------------- "Laugh, and the world laughs with you. Cry, and they will kick you in the fuc**ng teeth." -TDog
|
TDog
Buddy


Registered: 06/23/18
Posts: 227
Loc: Corner of Laydown and Sta...
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: TDog]
#25296071 - 06/27/18 11:25 PM (5 years, 6 months ago) |
|
|
I may end up adapting the wide mouth quart mason jars I have and using bottled (sterile) CO2 hooked to a regulated hand control/trip to pour shots/doses.
tvcasualty touched on that concept in passing as well.
-------------------- "Laugh, and the world laughs with you. Cry, and they will kick you in the fuc**ng teeth." -TDog
|
boom boom

Registered: 02/27/18
Posts: 429
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: TDog]
#25296087 - 06/27/18 11:38 PM (5 years, 6 months ago) |
|
|
Yeah being squeezable would rule. Spawn bags are probably the closest to being best of all worlds, but they are disposable and inoculation isn't as smooth as it is with jars. Some of them have SHIPs on them but that's kind of sketchy too. A spawn bag with a female luer attachment would be cool. You could just take off the cap and directly connect a syringe to the bag itself. Anyways, you're probably overthinking it.
|
boom boom

Registered: 02/27/18
Posts: 429
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: boom boom]
#25296103 - 06/27/18 11:50 PM (5 years, 6 months ago) |
|
|
As far as lc/li, I think morelmans concept is probably going to be at the center of anything that would fit what you're trying to do. There's still transfers to syringes, but that are super clean transfers and sterile technique is a breeze with them. There's all sorts of different things you can do with different designs that incorporate his design
https://www.shroomery.org/forums/showflat.php/Number/25287718
Josex also has some sort of MacGyver jar he's been using. It's on the last page of the post your new tools of the trade thread.
Edited by boom boom (06/27/18 11:52 PM)
|
TDog
Buddy


Registered: 06/23/18
Posts: 227
Loc: Corner of Laydown and Sta...
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: boom boom]
#25296181 - 06/28/18 01:30 AM (5 years, 6 months ago) |
|
|
(*Edited - wrong term used once - replaced.*)
(*Edit #2 - the item linked below was listed wrong by the company - they sent something different than the listing - just got off the phone with customer service - looks like it's just going to be a matter of "send it back and get your money back" - will update if they actually end up honoring the listing [I HIGHLY doubt that they will though] - They are definitely correcting the listing now.*)
I will definitely incorporate that in. That's a neat idea.
As far as over-thinking goes... that's my process. Brainstorming. I usually try not to state my trains of thought until I "git 'er dun", but since the base vessel is all that is holding me back I figured I would reach out.
I'm in contact with plastic suppliers and researching manufacturing processes. The more things I can gain exposure to as I go the better my result will be in the end. Usually it IS something simple, but I have to see all the trees in the forest before I find ground high enough to see the forest for the trees.
I've been down the road with bags, etc. but the "slurry-in-a-hurry" is my newest obsession. Once that first cake is up it's 3 days to make an unlimited number of jars (depending on how much base is available for use) and/or (and this is what I'm hyped about >>>) going straight to the substrate and having it colonized in 3 days.
With 15 x 90 quart monotubs and 10 x 58 quart monotubs, being able to do that is absolutely phenomenal.
I've always been ahead of the production line. This will take it to a whole new level though. I will need to step things up instead of "hurry up and wait" as per the usual methods.
I'm also working on (design is finished) an "air squid" (squirrel cage fan with prefilter ducted to a square HEPA box with a home-made circular manifold to attach lines to each tub on the high side to vent them and then pulling the exhaust out of the tent and venting outside).
And also a "Mr. Mister" (aka "Sir Dunksalot") adapter for my tubs. (Design is almost finished.)
Another little doo-dad I put together (easy stuff) was a UV sterilizer holding box.
Adapted some old forum posts to make a (almost pro level) glove box.
Plus I adapted the "Pinky Phish Filters" (big ups to eatyualive, again, for that material discovery and use of) to a "Tubular Tek" where I put rubber grommets on my FAE ports and place short 1 1/2" PVC pipes in them to house the filters. PVC caps on for incubation and removed for fruiting (I am not a fan of the magnetic caps and the "air squid" hooks to the top intake ports OR the tub can be run without the "air squid" if one chooses).
I applied this over-thinking type approach to my years of... botany... and it paid off in HUGE ways.
In any case I will share what I have when that day comes.
Lots to do until then.
(And thank you for your link and trying to help. I appreciate any input from people who KNOW stuff.)
Since I am asking for help with the base vessel I will add help with this tip... I think they may have mis-priced these... look them up anywhere else (eBay, Amazon, Google... anywhere) and you will see what I mean. Even the only other seller at the moment on the same site has this marked exponentially higher. (Free shipping on orders over $35 as well.) The five I ordered will be here soon. (I'm reselling most of those suckers. Lol.)
xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
***** SEE "EDIT #2" AT THE TOP OF THIS POST *****
https://www.walmart.com/ip/Waterwise-7000-3-Countertop-Water-Distiller/135009067
xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx
Just the tip. Lolz.
Alrighty... I've been keeping Mab waiting long enough...
-------------------- "Laugh, and the world laughs with you. Cry, and they will kick you in the fuc**ng teeth." -TDog
Edited by TDog (06/28/18 02:24 PM)
|
boom boom

Registered: 02/27/18
Posts: 429
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: TDog]
#25296475 - 06/28/18 07:30 AM (5 years, 6 months ago) |
|
|
Quote:
TDog said: As far as over-thinking goes... that's my process. Brainstorming. I usually try not to state my trains of thought until I "git 'er dun", but since the base vessel is all that is holding me back I figured I would reach out.
Overthinking without having knowledge and experience seems to end poorly in mycology, since people tend to overthink based on their own stereotypes and what they believe the conventional wisdom is. But most of the laymans knowledge about growing mushrooms is rooted in misconceptions. For instance, you're talking about this big, elaborate set up for providing filtered air to your tubs, but spawning to bulk isn't a sterile process. Contaminants in the tub phase are based on dirty spawn and improperly pasteurized/sterilized substrate. Filtered air not only does little to nothing to prevent it, but would be totally unproven in regard to surface conditions and gas exchange, which are the main factors in determining yield. You'd be wandering into the unknown in the hopes that you'll have successfully reinvented the wheel at the end of the process. The end result of such projects is often a very expensive experiment that ends up being outperformed by the guy who skimmed a tek and just spawned to bulk in a half assed 6 hole mono and ignored it until it fruited. The established teks in these forums are the culmination of decades of experience and trial and error. If you just look at them like bah, that's unprofessional noob shit, you're likely to end up with more headaches than breakthroughs.
Quote:
I've been down the road with bags, etc. but the "slurry-in-a-hurry" is my newest obsession. Once that first cake is up it's 3 days to make an unlimited number of jars (depending on how much base is available for use) and/or (and this is what I'm hyped about >>>) going straight to the substrate and having it colonized in 3 days.
With 15 x 90 quart monotubs and 10 x 58 quart monotubs, being able to do that is absolutely phenomenal.
Inoculant is easy to make in bulk. One jar with 400ml of lc could end up inoculating 200+ quarts for like 25 dry pounds, and you can prepare that in like an afternoon with a plate of colonized agar. That's the easy part.
As far as slurry straight to sub, the amount of spawn used is what determines yields, not the size of the tub or the amount of substrate, so unless you're talking about slurry by the gallon, I don't think there's a way to avoid making spawn.
|
TDog
Buddy


Registered: 06/23/18
Posts: 227
Loc: Corner of Laydown and Sta...
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: boom boom]
#25296852 - 06/28/18 11:46 AM (5 years, 6 months ago) |
|
|
*Edited - one typo - "emboldened" and capitalized one word - parenthetical mistake.*
*Edit # 2 - one typo - redundant phrasing modified.*
-
"Overthinking without having knowledge and experience seems to end poorly in mycology, since people tend to overthink based on their own stereotypes and what they believe the conventional wisdom is. But most of the laymans knowledge about growing mushrooms is rooted in misconceptions. For instance, you're talking about this big, elaborate set up for providing filtered air to your tubs, but spawning to bulk isn't a sterile process. Contaminants in the tub phase are based on dirty spawn and improperly pasteurized/sterilized substrate. Filtered air not only does little to nothing to prevent it, but would be totally unproven in regard to surface conditions and gas exchange, which are the main factors in determining yield. You'd be wandering into the unknown in the hopes that you'll have successfully reinvented the wheel at the end of the process. The end result of such projects is often a very expensive experiment that ends up being outperformed by the guy who skimmed a tek and just spawned to bulk in a half assed 6 hole mono and ignored it until it fruited. The established teks in these forums are the culmination of decades of experience and trial and error. If you just look at them like bah, that's unprofessional noob shit, you're likely to end up with more headaches than breakthroughs."
Who said I didn't have appropriate/accurate knowledge and/or experience? Because I choose to filter air that doesn't need to be filtered? Because I just started posting? You assume much.
I understand what you are saying.
I overkill on purpose.
I do these things so I don't get bored.
That air setup is actually not complicated. It just sounds that way.
And as far as the air being unnecessary to filter, you're right. But I'm not trying to poor-boy my lab either though. There are a lot of little details I could add right here but I will save it for the day that I post the complete "TDog's Dialed In (Unnecessary) Air Squid TEK".
"Expensive" is a relative term.
-
These are things to keep me thinking and interested. Otherwise I get bored and stop caring. It becomes a job instead of a hobby, and a bunch of day-in and day-out chores. For me anyway.
-
I'm not the type to compare d***s either. That is to say I'm not going to try and convince anyone of what I know or my level of competence. I'm not about to get in to the girl-fight slapping party that is rampant on these forums in regards to who knows more than who, etc. (That shuts down a productive exchange of thought.) The only reason I even say any of that, or replied to what you just posted with my statements sounding like they are "in my defense" is so we can get on the same footing here. (More or less me introducing myself better.)
-
In regards to the size and number of tubs it was simply to give an idea of the surface footprint and a reference to the potential of bulk slurry to bulk substrate if I were to cut my times down.
I like to stick with the Sterilite brand (I'm just like that), and I don't like/trust all the latch-top-models. That only leaves the "Basic Clears" and I am limited to dimensions based on the production set available as well as the space restrictions I have to work with/around.
I'm right there with you when you say that the size of the tub isn't what's important (<<<<< that's what she said?).
If I could make my own as well as they make theirs then I would. (Maybe one day... *wheels slowly begin spinning as my grey matter brings up decades-old memories of vacuum molding plastics and price lists for acrylic sheets*)
-
I have been out of this for a while but I know the tried and true stuff. I used to do it. (Like how most of the posts I see [on other forums especially] are all kinds of twisted up now where people are boiling Unicorn Bags full of substrate to pasteurize it - inside their home - in a giant pot that barely fits on their stove - that has a curved bottom denoting that it should be used outdoors on a propane burner - when there is a perfectly good electric oven being neglected just below the burner they are working on. That's why I was so excited when I saw some of the OG posters resurrect things like what eatyualive put up in regards to the old ways. If I was sitting here arguing about substrate depth producing more yields then yes... call me out. I never said filtered air was necessary though. I just like keeping things clean for the sake of keeping them clean is all. That "Air Squid" is for gas exchange and the setup itself is to keep things nice and neat as well as to avoid running a wet-vac across the tent floor every day or exhausting fog out the window and into the street every time I do an air exchange [no open trays and ultrasonic humidifiers]. [Also so I don't breathe all that CO2 in constantly.] There are of course so many little details in regards to so many other details involved in the reasons I have to run things like I do that mentioning them all would be tedious and boring not only to write but to read as well.)
-
This is also why I don't usually post.
(Admittedly though I got excited and opened my big fat mouth about all my little Willy-Wonka-Esque projects... it's hard for me to play things close to the vest these days [for a few reasons].)
-
"...unless you're talking about slurry by the gallon, I don't think there's a way to avoid making spawn."
I AM talking about gallons of slurry. That is EXACTLY what I'm talking about.
I want to trade those 60 x 1 quart mason jars (and my old way of using rye berries and syringes with standard methods and times) in for 100+ x 1 quart "Lord of the Jugs" end-all-be-all vessels that can do it all (or maybe 100+ half gallon [2000ml] bottles/jugs/jars :o *mad-scientist-laugh*).
I'm talking about making a new vessel that (while perhaps expensive, and at this point in the cohesive understating it IS unnecessary) can do everything... and well at that (while only having to open it once to put in the preliminary unsterilized media before autoclaving with respect to the majority of the tasks said vessel will be responsible for performing). Multiplied and replicated. (While disregarding the costs [to a point... I'm not about to go buy a $5k autoclave or anything... again, "expensive" is a relative term].)
-
I'm just trying to stay interested.
(INTJ-A personality type also means I can't really help it. I'm going to think about all this stuff whether I want to or not... so I usually have to act on it or I will just think about it forever.)
-
"If you just look at them like bah, that's unprofessional noob shit, you're likely to end up with more headaches than breakthroughs."
I would NEVER look at these TEK's in that regard.
It makes me wonder how you would get that impression from me.
If I sounded that way I assure you it was not my intent. Not in the slightest.
That is also why I posted to this (old) thread. It's out of respect to these things that I asked my question in THIS "temple". I hold these methods (and especially eatyualive, tvcasualty, and The Tribe) in VERY high regard.
(I would like to add that I stayed up many a night [well over a decade ago] reading through agar's posts. MANY a night.)
I have nothing but respect for the proven TEK's.
-
I do understand what you are saying though. I do. I promise.
Your words are not lost on me.
I also would like to say that I appreciate your input.
You are the only one responding so there's that, too. Lol.
I'm not attempting to undermine your statements. What you have said is valid (minus the assumptions... but that's just kind of what everyone has to do since we don't know each other).
And I would also like to state that yes, I am new to the "slurry-in-a-hurry" method and all that entails; BUT, I am doing what you said in your opening statements involving your latest reply. (Meaning I am doing the old stuff... I'm just looking down the road as I bastardize a protein shake bottle and some replacement parts for a $10 blender is all. Lol.)
-
You are also right that it may all be for naught. It may be a year of me tinkering and in the end just walking away from it.
I will also learn more along my way/journey than I would have otherwise, and perhaps that can/will be applied to future projects/branches. (Even if the knowledge gained here goes in to some other aspect/realm of something "unrelated" to any of this.)
I just like to do stuff like this too I guess.
(And if you would like to move our discussion to private messages I'm down for that as well. As I said, you're the only one responding [anyway]. There is actually something I would like to ask you that is best suited for a different thread, but I feel we are becoming better acquainted and since you are familiar with things, and responding/active, it would be foolish for me to cast my line back in to an ocean of salt at this point.)
-
TIME is the most valuable, costly (priceless), precious, and important thing we have (IMHO). I promise you that you are not wasting yours in your efforts with me.
-
(I also like to end with a joke if I can.)
Patches gets it.
Edited by TDog (06/28/18 12:26 PM)
|
TDog
Buddy



Registered: 06/23/18
Posts: 227
Loc: Corner of Laydown and Sta...
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: TDog]
#25305107 - 07/02/18 06:31 PM (5 years, 6 months ago) |
|
|
*Edit 1 - Changed color of some text (could not be easily read). Changed a punctuation error.
-----
PP5.
That's my lead as far as a material type.
(I never would have thought that the unassuming ziplock and glad containers were made of that.)
Thanks to elasticaltiger (stumbled across that little bit of information while reading through his stuff regarding the "Tiger Drop" - and if you ever see this, I'm an INTJ-A ;p props, yo).
Back to it then.
Edited by TDog (07/02/18 07:20 PM)
|
TDog
Buddy



Registered: 06/23/18
Posts: 227
Loc: Corner of Laydown and Sta...
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: TDog]
#25305179 - 07/02/18 07:15 PM (5 years, 6 months ago) |
|
|
*Edit 1 - Messed up text coloring. Fixed.
-----
There's this as well... however, as with most things, it is not specific enough. There are types within types that it says absolutely nothing about. It is a lead though.
(It's like how TYVEK has SO MANY variants...)
http://www.used-autoclave-s.com/autoclavable/
Back to it.
P.s. I promise I will read the writeup on standard forum posting formats, color usage, and linking/quoting. Lol. So much to do...
-------------------- "Laugh, and the world laughs with you. Cry, and they will kick you in the fuc**ng teeth." -TDog
Edited by TDog (07/02/18 07:22 PM)
|
TDog
Buddy



Registered: 06/23/18
Posts: 227
Loc: Corner of Laydown and Sta...
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: TDog]
#25310586 - 07/05/18 07:59 PM (5 years, 6 months ago) |
|
|
With my current setup everything is leaning towards 1/2 gallon - 2 quart - 64oz Mason Jars.
Putting blender attachments on those and scheming on other port attachments, filters, port protectors... always "thinkering"... always.
Looking forward to the day.
-------------------- "Laugh, and the world laughs with you. Cry, and they will kick you in the fuc**ng teeth." -TDog
|
TDog
Buddy



Registered: 06/23/18
Posts: 227
Loc: Corner of Laydown and Sta...
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: TDog]
#25319544 - 07/10/18 09:07 PM (5 years, 6 months ago) |
|
|
I found something...
https://www.usplastic.com/catalog/item.aspx?sku=68082&gclid=EAIaIQobChMI8Ojwg4aW3AIVCJRpCh0ZtAmnEAQYASABEgIUhfD_BwE
:O
THAT is the current winner in my prototype book...
-
JIC the link breaks:
1 Gallon Nalgene™ Polypropylene Large Wide Mouth Bottle Item #: 68082 PPCO bottle Polypropylene linerless cap Not recommended for shipping liquids Autoclavable Temperature range: -0°C to 135°C Meets FDA standards Cap is included Price: $32.29
Cap Size: 100mm, Neck ID: 89mm, Dimensions: 153mm Diameter x 288mm Height
Discounts: Qty 6: 5% off; Qty 12: 10% off; Qty 36: 15% off;
-
A dream for another day.
-------------------- "Laugh, and the world laughs with you. Cry, and they will kick you in the fuc**ng teeth." -TDog
|
Solipsis
m̶a̶d̶ disappointed scientist



Registered: 12/28/09
Posts: 3,398
Loc: the Neitherlands
Last seen: 5 months, 19 days
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: TDog]
#25319913 - 07/11/18 04:53 AM (5 years, 6 months ago) |
|
|
Quote:
TDog said:
It's possible there is a 3D printer filament out there that would work as well. I'm not sure.
I would even take suggestions of what type of base material would work if it's mold poured, etc.
https://3dprint.com/7279/3d-print-fungus-mycelium/
Other than that, not sure what you mean by mycelium water - not LC?
I am testing a piece of reishi mycelium immersed in water to check for conditions to grow laminated leather-like myc growth and I swear if it sits in water for about 2-3 days (open to the air by the way) it starts smelling like artificial orange flavor lol
I'm told some Pleurotus water can smell really funky but also fresh!
|
Col_ClusterFock
Stranger
Registered: 06/18/18
Posts: 2
Last seen: 5 months, 13 days
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: Solipsis]
#25362685 - 08/02/18 05:53 PM (5 years, 5 months ago) |
|
|
Kinda old thread but found something cool people might use.
https://www.usplastic.com/mobile/catalog/item.aspx?itemid=25126&categoryid=617
Polypropylene jars with regular mouth mason jar threads.
Could use the sip straw mason lids, or I was looking at using these:
https://www.amazon.com/gp/aw/d/B0789GV76F/ref=ya_aw_od_pi?ie=UTF8&psc=1
Add a SHIP on that mouthpiece flipper lid and a syringe filter, oster blade at the bottom, you could have something for pouring or using a syringe for precise measurements.
|
L0rdk4in
A noob


Registered: 05/12/14
Posts: 113
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#25643922 - 11/27/18 10:32 PM (5 years, 2 months ago) |
|
|
I tried this a year or so ago but just had trouble getting my base to adhere with out leaks. Coming back to the hobby recently and I still want to make this work. Preferably with the random gear I already bought last time. I did sand the base down and I have tried both clear silicon and red rtv. I know I needed to clean up the blender base before I try again. This is a the smaller blender bottle but I don't know if that matters,
|
Mateja


Registered: 07/14/16
Posts: 7,948
Loc: Here
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: L0rdk4in] 1
#25643967 - 11/27/18 11:09 PM (5 years, 2 months ago) |
|
|
You can do this method by dropping an agar wedge into a jar with a normal lid and just shake it manually for 10-20 seconds. This method I'm describing is called Blenderless Liquid Inoculant and I use it often. Even tho your myc water looks like it has 1 tiny visible strand of myc and nothing else in it, I assure you that every ml of water contains thousands of invisible to the naked eye myc fragments. 100 times more easier than dealing with all the extra stuff IMO and much safer as well. Gl
-------------------- Cakes inside Water Tub
|
L0rdk4in
A noob


Registered: 05/12/14
Posts: 113
|
Re: Mycelium Water, A Tribal Container [beta version] [Re: Mateja]
#25651591 - 12/01/18 10:29 PM (5 years, 1 month ago) |
|
|
Thanks!! Yea that sounds so awesome and easy. I'll do one and maybe even try the tribal shaker bottle and modified lid as a second one.I mean I already made the pour lid.
|
|