Mycelium Water, A Tribal Container 






Blending, pouring and/or making syringes with one jar


The goal is to make an eberbach like jar with a self-healing injection port to suck LI into syringe and a pour system for the pf-slurry.
I like the pour plug system to pour LI AND SLURRY because its very easy to use and can be a bit more precice when i pour into grain jars
and feel safer with the smaller opening compared to pouring strait from the jar, especially when i noc 30-40 jars with a batch

This is the beta version, some tweakage may come.

Jar Construction


i'll mostly let the picture talk
Things you'll need:

• ball sip and straw lids
  
• ball freezer jars 16oZ or similar pp5 container
  
• heavy duty self-healing injection ports
  
• an oster blender blade with the seal
  
• 2" hole saw
  
• 1/2" drill bit
  
• small saw or dremel
  
• a small magnet or some type of screw for the plug(be creative;))
  
• silicone
  
• sandpaper
  

Place the lid offset near the pour hole to be closest of the side of the jar to reduce wastage, (maybe optional)


The white lid in this pic is not the one i used, i cutted it too small
See where i made the lines, there is a kind of a rim, you want to keep the most of this rim because this is what will hold the white sip'n'straw lid






1/2" spade drill bit to make the SHIP holes, for the plug you'll have to make it a bit bigger for easy pulling


Sand the edge so the silicone stick well, i place the lid on a tape roll and apply the silicone, wait until it dry and make the other side


Hole saw 2", mine isn't very good so i predrill to avoid cracking and again, dont forget to sand the base before applying silicone


also dont forget to sand the oster blade, because thats what will happen if not
one PC cycle and it peel off..

so sand where you will apply silicone,


Once its mostly dried, i put the seal on the bottom for stability



A small magnet is glued to one of the SHIP (the plug)


In my glove, i place a small knot or washer hardware or even a dime, anything magnetic enough so the plug stick to my finger to pull it off
thanx tourrat for the magnet idea


Once everything is completely dry, put water in it to test the sealing, the only place you should see leaks is from the pour plug
but its ok because its for immediate use



using the container


Fill at 1/2 or 2/3 of the container with tap water, depending on how much inoculum you want, screw tightly, cover with foil, pc for 30min and follow your favorite liquid/slurry inoculant tek
like these
3 Day Quart Colonization: Slurry Liquid Spawn or Inoculant
Liquid Inoculant ( NEW version)
MY MudaFukan BOTTLE TEK( as easy as it gets)
Updated Bottle Tek

Pouring with the container

This is an over colonized AA+ master jar, i was unable to break the chunk for g2g so i though it was good candidate for a slurry!

i had hard time because the small knot in my glove had not enough magnetic force to be able to pull the plug properly
i'll make the hole slightly bigger and use something like a dime next time.

      Results!   



48 hrs after inoculation of WBS, 72 hours, 84hrs(shaking),  spawned on day 5


tub 1


Tub 2, i had a small contam spot in top but surgery went well




Jar types

Now for the type of jar you are not limited with those model

but i highly suggest you this model with a screw lid:

over this model with a snap lid:

There is also these Blender Bottles that look perfect for the job!

Dhearic said:

,
, ,

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So this is it for now!
Good luck in your Liquids inoculants and slurries adventures!!





Brought to you by THE TRIBE

Liquid Inoculant and GS2G(Grain Slurry to Grain Transfer) Below

Now normally I was using a 2:1 grain to water ratio for these grain slurries as liquid inoculant. However, I wanted to try a smaller volume of grain to see how far a quart jar really can be expanded. This example shows how a GLC(Grainwater Liquid Culture) was basically extracted from a spoonful of grain. Once blended the mycelial water was poured into receiving quart grain jars for transfer. None of the grains from the GLC jar were poured into receiving jars this time. My normal GS2G method involves making more of a mud consistency blend and pouring the grains and blended water into the receiving jars.

One spoonful of a colonized quart jar was used per pint of water for the Tribal Slurry(GS2G) illustrated below. 1 plate to 1/4 pint of water was used for the Liquid Inoculant below.

So my GLC and LI jars are almost finished. The LI jars won slightly. I had to shake both because they were taking forever(more than 4 days). Im spoiled by slurry and the 2:1 grain to water slurries.  For pure mycelial water, the colonization was as fast as the LI from a grain jar. 9 days full colonization. This is slow on my end for g2g.

Grain slurry and pf jar slurry are take half the time to colonize jars wothout a shake. The LI and GLC took 9 days with a shake on day 5 to colonize 100%. Although ive compared them several times.  I will still continue to do so in this thread.

Day 1-9: Only 20 quarts were used with LI. 30 used with the GLC. the jars on the right of the colonized quart are a Syzygy clone. Jars on the left are an amazon clone. The quart jars were all shaken on day 5.



GLC Amazon

Day of spawning


LI Syzygy



Liquid Inoculant: 1 pint water to 1 plate agar

A regular mouth quart jar was fitted with a blade assembly and filled with 1 pint of water. The Tribal lid for this jar was wrapped in foil and pressure cooked for 20 minutes at 15psi. The plate was dropped in the quart jar and blended for 2(3-5 second bursts). Taken back into the SAB. Then the lids were switched for pouring.

Normally, I used 1/4 pint of water per plate with the LI. This volume of water was 4 times the normal volume. In this trial the LI showed leap off on the 2nd day. This is Penis Envy.



to be updated...

Quote:

Normal procedure for GS2G transfers. Now incorporating the use of the Tribal Container to make the entire process streamlined, easy and less messy.

SLURRY TYPE 2:

GS2G Transfer(Grain Slurry to Grain jar transfer): wbs grain jar blended and poured into other grain jars.
A ratio of 1 pint grain spawn to 1/2 pint of water was used for 50 quarts of colonized grain.

Do all of this as sterile as possible. Research on sterile technique is assumed prior to this.

1. Fill a pint jar half full of distilled water and place the blade assembly on it. Pressure cook for 20 mins at 15psi. Allow jar to cool.
2. Prepare your clean area(Shmuvbox, SAB, Flowhood) and your substrates and tubs. Setup for spawning.
3. Using a phonebook, break up the grain inside the jar.
4. In your SAB, Take the colonized pint of grain loosen the lid. Loosen the lid of the sterile water pint jar.
5. pour water from water pint into grain pint.
6. place blade assembly lid on grain pint and water pint jar.
7. Blend on your oster blender base. Hold the jar in place with your hand while blending. I blend about 30 seconds.
8. In your SAB, Pour the grain slurry into your quarts jars. You pour by eye about 1 tablespoon volume of slurry per Quart Grain Jar.
9. Wait 3-4 days until full colonization. Give it a day or two more and then your ready to spawn your tubs. You can shake your grain a day before spawning to test for any contamination.

Regular Mouth Lids


Quarts and Pints(Pints will each be used as masters to slurry 100 more quarts each. In the last picture you can see all the colonized pints and 1 quart that i slurried. all the colonized jars are on the last bottom picture. each one of those pints will be used as a master for more than 30 jars at a time)judging from experience, each pint can do at least 200 quart jars of grain. i easily did 30 tonight and had to throw out 2/3rds of the pint jar of grain leftover.





SLURRY VS LIQUID INOCULANT

The Liquid inoculant(texan yellow cap clone-3rd transfer plate) was inoculated on 8-11-2015. The slurry(Thai Lipa Yai multispore pf jar) was inoculated on 8-16-2015. The Liquid inoculant is a fresh clone Texas Yellow Cap plate from agar. The slurry is a 1 years old multispore Thai Lipa Yai from a pf jar that had been sitting in the fridge from 2014.

As you can see the first image was taken on 8-18-2015. 2 days after the slurry was inoculated. The last picture was taken today. So the LI took roughly 8 days to colonize 99%. The slurry in 3 days has almost caught up with the LI. Neither set of jars has been shaken since inoculation.

If you zoom in you can see the tape label. The LI jars are to the left of the LI label. The Slurry jars are to the right of the label. So half the speed on grain. This is consistent for both LI and Slurry with a multitude of tests.

A little background on these cultures. the thai culture is the fastest growing cube culture i have. tex is 2nd fastest. very close in speed. but the thai is multispore and the tex is a clone. the thai pf jar sat in the fridge for over a year and still caught up to the fresh tex clone culture.

check it out. day 4 on the slurry almost done. caught right up to the LI that had a 5 day head start.

Darkhome said:

You guys are Amazing! New ideas are what make advances in this hobby possible!

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No YoU are amazing!!!

haha thanks mate!


eatyualive... You tha man!!!


The video of the inoculation in the OP been done 2 days ago and its showing good recovery.
I will update the grow to the OP as things progress.
Other member's built and other options/ideas will also be added to the OP as things move on

cheers

Dhearic said:

Doesn't leak and blends myc like a charm

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