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InvisibleTDog
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Registered: 06/23/18
Posts: 227
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Re: Mycelium Water, A Tribal Container [beta version] [Re: TDog]
    #25296060 - 06/27/18 11:17 PM (5 years, 6 months ago)

(If anyone knows of something like that I'm all ears.

I'm researching day in and day out.

It's possible there is a 3D printer filament out there that would work as well. I'm not sure.

I would even take suggestions of what type of base material would work if it's mold poured, etc.

Still looking. Daily.

I'm hoping that some obscure vessel exists already. Maybe on some scientific website that is buried in the web.

That one I linked would be perfect if it could stand up to the heat(/time).
It probably has something to do with the softeners in the polypropylene they used, but I really do not know.

Still looking.

Hopefully Frodo didn't throw it in the volcano yet...)


--------------------
"Laugh, and the world laughs with you. Cry, and they will kick you in the fuc**ng teeth." -TDog


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InvisibleTDog
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Re: Mycelium Water, A Tribal Container [beta version] [Re: TDog]
    #25296071 - 06/27/18 11:25 PM (5 years, 6 months ago)

I may end up adapting the wide mouth quart mason jars I have and using bottled (sterile) CO2 hooked to a regulated hand control/trip to pour shots/doses.

tvcasualty touched on that concept in passing as well.


--------------------
"Laugh, and the world laughs with you. Cry, and they will kick you in the fuc**ng teeth." -TDog


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Invisibleboom boom

Registered: 02/27/18
Posts: 429
Re: Mycelium Water, A Tribal Container [beta version] [Re: TDog]
    #25296087 - 06/27/18 11:38 PM (5 years, 6 months ago)

Yeah being squeezable would rule. Spawn bags are probably the closest to being best of all worlds, but they are disposable and inoculation isn't as smooth as it is with jars. Some of them have SHIPs on them but that's kind of sketchy too. A spawn bag with a female luer attachment would be cool. You could just take off the cap and directly connect a syringe to the bag itself. Anyways, you're probably overthinking it.


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Invisibleboom boom

Registered: 02/27/18
Posts: 429
Re: Mycelium Water, A Tribal Container [beta version] [Re: boom boom]
    #25296103 - 06/27/18 11:50 PM (5 years, 6 months ago)

As far as lc/li, I think morelmans concept is probably going to be at the center of anything that would fit what you're trying to do. There's still transfers to syringes, but that are super clean transfers and sterile technique is a breeze with them. There's all sorts of different things you can do with different designs that incorporate his design

https://www.shroomery.org/forums/showflat.php/Number/25287718

Josex also has some sort of MacGyver jar he's been using. It's on the last page of the post your new tools of the trade thread.


Edited by boom boom (06/27/18 11:52 PM)


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InvisibleTDog
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Re: Mycelium Water, A Tribal Container [beta version] [Re: boom boom]
    #25296181 - 06/28/18 01:30 AM (5 years, 6 months ago)

(*Edited - wrong term used once - replaced.*)

(*Edit #2 - the item linked below was listed wrong by the company - they sent something different than the listing - just got off the phone with customer service - looks like it's just going to be a matter of "send it back and get your money back" - will update if they actually end up honoring the listing [I HIGHLY doubt that they will though] - They are definitely correcting the listing now.*)

I will definitely incorporate that in. That's a neat idea.

As far as over-thinking goes... that's my process. Brainstorming. I usually try not to state my trains of thought until I "git 'er dun", but since the base vessel is all that is holding me back I figured I would reach out.

I'm in contact with plastic suppliers and researching manufacturing processes. The more things I can gain exposure to as I go the better my result will be in the end. Usually it IS something simple, but I have to see all the trees in the forest before I find ground high enough to see the forest for the trees.

I've been down the road with bags, etc. but the "slurry-in-a-hurry" is my newest obsession. Once that first cake is up it's 3 days to make an unlimited number of jars (depending on how much base is available for use) and/or (and this is what I'm hyped about >>>) going straight to the substrate and having it colonized in 3 days.

With 15 x 90 quart monotubs and 10 x 58 quart monotubs, being able to do that is absolutely phenomenal.

I've always been ahead of the production line. This will take it to a whole new level though. I will need to step things up instead of "hurry up and wait" as per the usual methods.

I'm also working on (design is finished) an "air squid" (squirrel cage fan with prefilter ducted to a square HEPA box with a home-made circular manifold to attach lines to each tub on the high side to vent them and then pulling the exhaust out of the tent and venting outside).

And also a "Mr. Mister" (aka "Sir Dunksalot") adapter for my tubs. (Design is almost finished.)

Another little doo-dad I put together (easy stuff) was a UV sterilizer holding box.

Adapted some old forum posts to make a (almost pro level) glove box.

Plus I adapted the "Pinky Phish Filters" (big ups to eatyualive, again, for that material discovery and use of) to a "Tubular Tek" where I put rubber grommets on my FAE ports and place short 1 1/2" PVC pipes in them to house the filters. PVC caps on for incubation and removed for fruiting (I am not a fan of the magnetic caps and the "air squid" hooks to the top intake ports OR the tub can be run without the "air squid" if one chooses).

I applied this over-thinking type approach to my years of... botany... and it paid off in HUGE ways.

In any case I will share what I have when that day comes.

Lots to do until then.

(And thank you for your link and trying to help. I appreciate any input from people who KNOW stuff.)

Since I am asking for help with the base vessel I will add help with this tip... I think they may have mis-priced these... look them up anywhere else (eBay, Amazon, Google... anywhere) and you will see what I mean. Even the only other seller at the moment on the same site has this marked exponentially higher. (Free shipping on orders over $35 as well.) The five I ordered will be here soon. (I'm reselling most of those suckers. Lol.)


xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

***** SEE "EDIT #2" AT THE TOP OF THIS POST *****

https://www.walmart.com/ip/Waterwise-7000-3-Countertop-Water-Distiller/135009067

xxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxxx

Just the tip. Lolz.

Alrighty... I've been keeping Mab waiting long enough...


--------------------
"Laugh, and the world laughs with you. Cry, and they will kick you in the fuc**ng teeth." -TDog


Edited by TDog (06/28/18 02:24 PM)


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Invisibleboom boom

Registered: 02/27/18
Posts: 429
Re: Mycelium Water, A Tribal Container [beta version] [Re: TDog]
    #25296475 - 06/28/18 07:30 AM (5 years, 6 months ago)

Quote:

TDog said:
As far as over-thinking goes... that's my process. Brainstorming. I usually try not to state my trains of thought until I "git 'er dun", but since the base vessel is all that is holding me back I figured I would reach out.




Overthinking without having knowledge and experience seems to end poorly in mycology, since people tend to overthink based on their own stereotypes and what they believe the conventional wisdom is. But most of the laymans knowledge about growing mushrooms is rooted in misconceptions. For instance, you're talking about this big, elaborate set up for providing filtered air to your tubs, but spawning to bulk isn't a sterile process. Contaminants in the tub phase are based on dirty spawn and improperly pasteurized/sterilized substrate. Filtered air not only does little to nothing to prevent it, but would be totally unproven in regard to surface conditions and gas exchange, which are the main factors in determining yield. You'd be wandering into the unknown in the hopes that you'll have successfully reinvented the wheel at the end of the process. The end result of such projects is often a very expensive experiment that ends up being outperformed by the guy who skimmed a tek and just spawned to bulk in a half assed 6 hole mono and ignored it until it fruited. The established teks in these forums are the culmination of decades of experience and trial and error. If you just look at them like bah, that's unprofessional noob shit, you're likely to end up with more headaches than breakthroughs.

Quote:


I've been down the road with bags, etc. but the "slurry-in-a-hurry" is my newest obsession. Once that first cake is up it's 3 days to make an unlimited number of jars (depending on how much base is available for use) and/or (and this is what I'm hyped about >>>) going straight to the substrate and having it colonized in 3 days.

With 15 x 90 quart monotubs and 10 x 58 quart monotubs, being able to do that is absolutely phenomenal.




Inoculant is easy to make in bulk. One jar with 400ml of lc could end up inoculating 200+ quarts for like 25 dry pounds, and you can prepare that in like an afternoon with a plate of colonized agar. That's the easy part.

As far as slurry straight to sub, the amount of spawn used is what determines yields, not the size of the tub or the amount of substrate, so unless you're talking about slurry by the gallon, I don't think there's a way to avoid making spawn.





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InvisibleTDog
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Re: Mycelium Water, A Tribal Container [beta version] [Re: boom boom]
    #25296852 - 06/28/18 11:46 AM (5 years, 6 months ago)

*Edited - one typo - "emboldened" and capitalized one word - parenthetical mistake.*

*Edit # 2 - one typo - redundant phrasing modified.*

-

"Overthinking without having knowledge and experience seems to end poorly in mycology, since people tend to overthink based on their own stereotypes and what they believe the conventional wisdom is. But most of the laymans knowledge about growing mushrooms is rooted in misconceptions. For instance, you're talking about this big, elaborate set up for providing filtered air to your tubs, but spawning to bulk isn't a sterile process. Contaminants in the tub phase are based on dirty spawn and improperly pasteurized/sterilized substrate. Filtered air not only does little to nothing to prevent it, but would be totally unproven in regard to surface conditions and gas exchange, which are the main factors in determining yield. You'd be wandering into the unknown in the hopes that you'll have successfully reinvented the wheel at the end of the process. The end result of such projects is often a very expensive experiment that ends up being outperformed by the guy who skimmed a tek and just spawned to bulk in a half assed 6 hole mono and ignored it until it fruited. The established teks in these forums are the culmination of decades of experience and trial and error. If you just look at them like bah, that's unprofessional noob shit, you're likely to end up with more headaches than breakthroughs."

Who said I didn't have appropriate/accurate knowledge and/or experience? Because I choose to filter air that doesn't need to be filtered? Because I just started posting? You assume much.

I understand what you are saying.

I overkill on purpose.

I do these things so I don't get bored.

That air setup is actually not complicated. It just sounds that way.

And as far as the air being unnecessary to filter, you're right. But I'm not trying to poor-boy my lab either though. There are a lot of little details I could add right here but I will save it for the day that I post the complete "TDog's Dialed In (Unnecessary) Air Squid TEK".

"Expensive" is a relative term.

-

These are things to keep me thinking and interested. Otherwise I get bored and stop caring. It becomes a job instead of a hobby, and a bunch of day-in and day-out chores. For me anyway.

-

I'm not the type to compare d***s either. That is to say I'm not going to try and convince anyone of what I know or my level of competence. I'm not about to get in to the girl-fight slapping party that is rampant on these forums in regards to who knows more than who, etc. (That shuts down a productive exchange of thought.) The only reason I even say any of that, or replied to what you just posted with my statements sounding like they are "in my defense" is so we can get on the same footing here. (More or less me introducing myself better.)

-

In regards to the size and number of tubs it was simply to give an idea of the surface footprint and a reference to the potential of bulk slurry to bulk substrate if I were to cut my times down.

I like to stick with the Sterilite brand (I'm just like that), and I don't like/trust all the latch-top-models. That only leaves the "Basic Clears" and I am limited to dimensions based on the production set available as well as the space restrictions I have to work with/around.

I'm right there with you when you say that the size of the tub isn't what's important (<<<<< that's what she said?).

If I could make my own as well as they make theirs then I would. (Maybe one day... *wheels slowly begin spinning as my grey matter brings up decades-old memories of vacuum molding plastics and price lists for acrylic sheets*)

-

I have been out of this for a while but I know the tried and true stuff. I used to do it. (Like how most of the posts I see [on other forums especially] are all kinds of twisted up now where people are boiling Unicorn Bags full of substrate to pasteurize it - inside their home - in a giant pot that barely fits on their stove - that has a curved bottom denoting that it should be used outdoors on a propane burner - when there is a perfectly good electric oven being neglected just below the burner they are working on. That's why I was so excited when I saw some of the OG posters resurrect things like what eatyualive put up in regards to the old ways. If I was sitting here arguing about substrate depth producing more yields then yes... call me out. I never said filtered air was necessary though. I just like keeping things clean for the sake of keeping them clean is all. That "Air Squid" is for gas exchange and the setup itself is to keep things nice and neat as well as to avoid running a wet-vac across the tent floor every day or exhausting fog out the window and into the street every time I do an air exchange [no open trays and ultrasonic humidifiers]. [Also so I don't breathe all that CO2 in constantly.] There are of course so many little details in regards to so many other details involved in the reasons I have to run things like I do that mentioning them all would be tedious and boring not only to write but to read as well.)

-

This is also why I don't usually post.

(Admittedly though I got excited and opened my big fat mouth about all my little Willy-Wonka-Esque projects... it's hard for me to play things close to the vest these days [for a few reasons].)

-

"...unless you're talking about slurry by the gallon, I don't think there's a way to avoid making spawn."

I AM talking about gallons of slurry. That is EXACTLY what I'm talking about.

I want to trade those 60 x 1 quart mason jars (and my old way of using rye berries and syringes with standard methods and times) in for 100+ x 1 quart "Lord of the Jugs" end-all-be-all vessels that can do it all (or maybe 100+ half gallon [2000ml] bottles/jugs/jars :o *mad-scientist-laugh*).

I'm talking about making a new vessel that (while perhaps expensive, and at this point in the cohesive understating it IS unnecessary) can do everything... and well at that (while only having to open it once to put in the preliminary unsterilized media before autoclaving with respect to the majority of the tasks said vessel will be responsible for performing). Multiplied and replicated. (While disregarding the costs [to a point... I'm not about to go buy a $5k autoclave or anything... again, "expensive" is a relative term].)

-

I'm just trying to stay interested.

(INTJ-A personality type also means I can't really help it. I'm going to think about all this stuff whether I want to or not... so I usually have to act on it or I will just think about it forever.)

-

"If you just look at them like bah, that's unprofessional noob shit, you're likely to end up with more headaches than breakthroughs."

I would NEVER look at these TEK's in that regard.

It makes me wonder how you would get that impression from me.

If I sounded that way I assure you it was not my intent. Not in the slightest.

That is also why I posted to this (old) thread. It's out of respect to these things that I asked my question in THIS "temple". I hold these methods (and especially eatyualive, tvcasualty, and The Tribe) in VERY high regard.

(I would like to add that I stayed up many a night [well over a decade ago] reading through agar's posts. MANY a night.)

I have nothing but respect for the proven TEK's.

-

I do understand what you are saying though. I do. I promise.

Your words are not lost on me.

I also would like to say that I appreciate your input.

You are the only one responding so there's that, too. Lol.

I'm not attempting to undermine your statements. What you have said is valid (minus the assumptions... but that's just kind of what everyone has to do since we don't know each other).

And I would also like to state that yes, I am new to the "slurry-in-a-hurry" method and all that entails; BUT, I am doing what you said in your opening statements involving your latest reply. (Meaning I am doing the old stuff... I'm just looking down the road as I bastardize a protein shake bottle and some replacement parts for a $10 blender is all. Lol.)

-

You are also right that it may all be for naught. It may be a year of me tinkering and in the end just walking away from it.

I will also learn more along my way/journey than I would have otherwise, and perhaps that can/will be applied to future projects/branches. (Even if the knowledge gained here goes in to some other aspect/realm of something "unrelated" to any of this.)

I just like to do stuff like this too I guess.

(And if you would like to move our discussion to private messages I'm down for that as well. As I said, you're the only one responding [anyway]. There is actually something I would like to ask you that is best suited for a different thread, but I feel we are becoming better acquainted and since you are familiar with things, and responding/active, it would be foolish for me to cast my line back in to an ocean of salt at this point.)

-

TIME is the most valuable, costly (priceless), precious, and important thing we have (IMHO). I promise you that you are not wasting yours in your efforts with me.

-

(I also like to end with a joke if I can.)

Patches gets it.



Edited by TDog (06/28/18 12:26 PM)


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InvisibleTDog
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Re: Mycelium Water, A Tribal Container [beta version] [Re: TDog]
    #25305107 - 07/02/18 06:31 PM (5 years, 6 months ago)

*Edit 1 - Changed color of some text (could not be easily read). Changed a punctuation error.

-----

PP5.

That's my lead as far as a material type.

(I never would have thought that the unassuming ziplock and glad containers were made of that.)

Thanks to elasticaltiger (stumbled across that little bit of information while reading through his stuff regarding the "Tiger Drop" - and if you ever see this, I'm an INTJ-A ;p props, yo).

Back to it then.


Edited by TDog (07/02/18 07:20 PM)


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InvisibleTDog
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Re: Mycelium Water, A Tribal Container [beta version] [Re: TDog]
    #25305179 - 07/02/18 07:15 PM (5 years, 6 months ago)

*Edit 1 - Messed up text coloring. Fixed.

-----

There's this as well... however, as with most things, it is not specific enough. There are types within types that it says absolutely nothing about. It is a lead though.

(It's like how TYVEK has SO MANY variants...)

http://www.used-autoclave-s.com/autoclavable/

Back to it.


P.s. I promise I will read the writeup on standard forum posting formats, color usage, and linking/quoting. Lol. So much to do...


--------------------
"Laugh, and the world laughs with you. Cry, and they will kick you in the fuc**ng teeth." -TDog


Edited by TDog (07/02/18 07:22 PM)


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InvisibleTDog
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Re: Mycelium Water, A Tribal Container [beta version] [Re: TDog]
    #25310586 - 07/05/18 07:59 PM (5 years, 6 months ago)

With my current setup everything is leaning towards 1/2 gallon - 2 quart - 64oz Mason Jars.

Putting blender attachments on those and scheming on other port attachments, filters, port protectors... always "thinkering"... always.

Looking forward to the day.


--------------------
"Laugh, and the world laughs with you. Cry, and they will kick you in the fuc**ng teeth." -TDog


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InvisibleTDog
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Re: Mycelium Water, A Tribal Container [beta version] [Re: TDog]
    #25319544 - 07/10/18 09:07 PM (5 years, 6 months ago)

I found something...

https://www.usplastic.com/catalog/item.aspx?sku=68082&gclid=EAIaIQobChMI8Ojwg4aW3AIVCJRpCh0ZtAmnEAQYASABEgIUhfD_BwE

:O

THAT is the current winner in my prototype book...

-

JIC the link breaks:

1 Gallon Nalgene™ Polypropylene Large Wide Mouth Bottle
Item #: 68082
PPCO bottle
Polypropylene linerless cap
Not recommended for shipping liquids
Autoclavable
Temperature range: -0°C to 135°C
Meets FDA standards
Cap is included
Price:
$32.29

Cap Size: 100mm, Neck ID: 89mm, Dimensions: 153mm Diameter x 288mm Height

Discounts: Qty 6: 5% off; Qty 12: 10% off; Qty 36: 15% off;

-

A dream for another day.


--------------------
"Laugh, and the world laughs with you. Cry, and they will kick you in the fuc**ng teeth." -TDog


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OfflineSolipsis
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Re: Mycelium Water, A Tribal Container [beta version] [Re: TDog]
    #25319913 - 07/11/18 04:53 AM (5 years, 6 months ago)

Quote:

TDog said:

It's possible there is a 3D printer filament out there that would work as well. I'm not sure.

I would even take suggestions of what type of base material would work if it's mold poured, etc.





https://3dprint.com/7279/3d-print-fungus-mycelium/

Other than that, not sure what you mean by mycelium water - not LC?

I am testing a piece of reishi mycelium immersed in water to check for conditions to grow laminated leather-like myc growth and I swear if it sits in water for about 2-3 days (open to the air by the way) it starts smelling like artificial orange flavor lol

I'm told some Pleurotus water can smell really funky but also fresh!


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OfflineCol_ClusterFock
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Registered: 06/18/18
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Re: Mycelium Water, A Tribal Container [beta version] [Re: Solipsis]
    #25362685 - 08/02/18 05:53 PM (5 years, 5 months ago)

Kinda old thread but found something cool people might use.

https://www.usplastic.com/mobile/catalog/item.aspx?itemid=25126&categoryid=617

Polypropylene jars with regular mouth mason jar threads.

Could use the sip straw mason lids, or I was looking at using these:

https://www.amazon.com/gp/aw/d/B0789GV76F/ref=ya_aw_od_pi?ie=UTF8&psc=1

Add a SHIP on that mouthpiece flipper lid and a syringe filter, oster blade at the bottom, you could have something for pouring or using a syringe for precise measurements.


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InvisibleL0rdk4in
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Registered: 05/12/14
Posts: 113
Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
    #25643922 - 11/27/18 10:32 PM (5 years, 2 months ago)

I tried this a year or so ago but just had trouble getting my base to adhere with out leaks. Coming back to the hobby recently and I still want to make this work. Preferably with the random gear I already bought last time. I did sand the base down and I have tried both clear silicon and red rtv. I know I needed to clean up the blender base before I try again. This is a the smaller blender bottle but I don't know if that matters,




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InvisibleMateja
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Re: Mycelium Water, A Tribal Container [beta version] [Re: L0rdk4in] * 1
    #25643967 - 11/27/18 11:09 PM (5 years, 2 months ago)

You can do this method by dropping an agar wedge into a jar with a normal lid and just shake it manually for 10-20 seconds. This method I'm describing is called Blenderless Liquid Inoculant and I use it often. Even tho your myc water looks like it has 1 tiny visible strand of myc and nothing else in it, I assure you that every ml of water contains thousands of invisible to the naked eye myc fragments. 100 times more easier than dealing with all the extra stuff IMO and much safer as well. Gl


--------------------
Cakes inside Water Tub


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InvisibleL0rdk4in
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Registered: 05/12/14
Posts: 113
Re: Mycelium Water, A Tribal Container [beta version] [Re: Mateja]
    #25651591 - 12/01/18 10:29 PM (5 years, 1 month ago)

Thanks!! Yea that sounds so awesome and easy. I'll do one and maybe even try the tribal shaker bottle and modified lid as a second one.I mean I already made the pour lid.


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