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InvisiblecArcace-x
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Mycelium Water, A Tribal Container [beta version] * 6
    #21927954 - 07/11/15 08:52 AM (8 years, 6 months ago)

:chief:  Mycelium Water, A Tribal Container  :chief:







Blending, pouring and/or making syringes with one jar


The goal is to make an eberbach like jar with a self-healing injection port to suck LI into syringe and a pour system for the pf-slurry.
I like the pour plug system to pour LI AND SLURRY because its very easy to use and can be a bit more precice when i pour into grain jars
and feel safer with the smaller opening compared to pouring strait from the jar, especially when i noc 30-40 jars with a batch

This is the beta version, some tweakage may come.


Jar Construction



i'll mostly let the picture talk
Things you'll need:
  • ball sip and straw lids
  • ball freezer jars 16oZ or similar pp5 container
  • heavy duty self-healing injection ports
  • an oster blender blade with the seal
  • 2" hole saw
  • 1/2" drill bit
  • small saw or dremel
  • a small magnet or some type of screw for the plug(be creative;))
  • silicone
  • sandpaper



Place the lid offset near the pour hole to be closest of the side of the jar to reduce wastage, (maybe optional)


The white lid in this pic is not the one i used, i cutted it too small
See where i made the lines, there is a kind of a rim, you want to keep the most of this rim because this is what will hold the white sip'n'straw lid






1/2" spade drill bit to make the SHIP holes, for the plug you'll have to make it a bit bigger for easy pulling


Sand the edge so the silicone stick well, i place the lid on a tape roll and apply the silicone, wait until it dry and make the other side


Hole saw 2", mine isn't very good so i predrill to avoid cracking and again, dont forget to sand the base before applying silicone


also dont forget to sand the oster blade, because thats what will happen if not
one PC cycle and it peel off..

so sand where you will apply silicone,


Once its mostly dried, i put the seal on the bottom for stability



A small magnet is glued to one of the SHIP (the plug)


In my glove, i place a small knot or washer hardware or even a dime, anything magnetic enough so the plug stick to my finger to pull it off
thanx tourrat for the magnet idea :wink:


Once everything is completely dry, put water in it to test the sealing, the only place you should see leaks is from the pour plug
but its ok because its for immediate use



using the container



Fill at 1/2 or 2/3 of the container with tap water, depending on how much inoculum you want, screw tightly, cover with foil, pc for 30min and follow your favorite liquid/slurry inoculant tek
like these
3 Day Quart Colonization: Slurry Liquid Spawn or Inoculant
Liquid Inoculant ( NEW version)
MY MudaFukan BOTTLE TEK( as easy as it gets)
Updated Bottle Tek

Pouring with the container

This is an over colonized AA+ master jar, i was unable to break the chunk for g2g so i though it was good candidate for a slurry!


i had hard time because the small knot in my glove had not enough magnetic force to be able to pull the plug properly
i'll make the hole slightly bigger and use something like a dime next time.


:chief:      Results!    :chief:




48 hrs after inoculation of WBS, 72 hours, 84hrs(shaking),  spawned on day 5


tub 1


Tub 2, i had a small contam spot in top but surgery went well




Jar types


Now for the type of jar you are not limited with those model

but i highly suggest you this model with a screw lid:

over this model with a snap lid:

There is also these Blender Bottles that look perfect for the job!


Quote:

Dhearic said:
,
, ,





So this is it for now!
Good luck in your Liquids inoculants and slurries adventures!!





Brought to you by THE TRIBE




Edited by cArcace-x (10/16/15 10:49 AM)


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Invisibleeatyualive
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Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x] * 1
    #21927969 - 07/11/15 08:57 AM (8 years, 6 months ago)


Snap Lid Build


So Carc suggested the first container. Unforunately, I didn't have it available in my area. So I had to use the other one with the snap lid
the only ones available near me at walmart, big lots and grocery store were the snap lid. i know they aren't recommended but i took some close up pictures of them. the lids have a real nice seal. the smaller 8oz were too small for the blender assembly. ill use those for food. but the 16oz almost fits the metal band lid for a regular mouth half pint on the bottom. it fits on the blender base! ill go ahead and build it. and if it doesn't fit on the base, ill use it for LI with plates. also the 8oz Ball brand are too narrow for the white lids to fit on. But they are nice little containers. i think this should work. the seal is tight and there is enough clear plastic on the lid to drill out and rtv the metal blade assembly to. i messed around a little bit and put the lid and pulled it off several times. i think i know where the complaint comes from. if you push one side down without doing it evenly it pushes air out. if you press around the top with a few fingers on all sides it seals perfectly. the bottom of this one appears to need a 2.25" hole saw. im excited to try this out soon.


i also found a pp5 with a wide lid on it that is pp5 at big lots. although the base is too wide for an oster i think the drill bit will work with blade attachment in the bottom. i might mess around with both of these. the lids do have a really nice seal on them. but it appears to be a pain in the ass to try and cut into it. im going to order the ones you suggest for the build. i have these until i return them so i thought i would at least take some close up images. i was also able to pickup some pickling lime as the grocery store had a nice little section for canning.


a tiny bit of pressure can crack these plastic jars. i didn't even use the drill and put a little pressure and the bottom cracked. so on the 2nd jar i used the small bit and worked my way up.  it worked perfectly.

i drilled small guide holes so that i could fit the saw in and then sawed evenly and sanded afterwards. here is before the glue dries.

glue ha set and now im just waiting until tomorrow to put it together. i had to make a slight modification with this lid. it wouldn't get the proper seal if i cut anymore towards the edge of the jar. so i had to fit the white lid into the rim of the green seal. i would have had to cut too far on this lid that it would effect the seal. so i kept it in the middle. i made two white lids. one with two blue plugs. one with 1 plug and i was siliconing in the small hole. i plan on alternating it depending on which method i use. if i want to suck it up into a syringe i will use the lid with the two blue ports. if i want to pour. ill use the lid with 1 blue port. im fairly certain this will work well. the only issue is the clear plastic on the lid and the green soft plastic. where the seal meets its extremely tough to cut through. i kept a small portion of the outside clear plstic to keep that seal and drop the white lid in.



i let it dry and sealed the inside of the lid. fin. ill test it tomorrow on the blender.



look what i got. these little magnets are stronger than the magnetic vent covers.



found one lid with this ship. glued the magnet in. i could barely separate those small magnets. extremely strong. i accidentally broke the lid by trying to drill this ship hole bigger. so i had to silicone it again.



so this worked out perfectly. i had to reseal it a few times. as i was running low on silicone when i made it. today it worked perfectly. i did an LI inoculated 15 quart jars from an agar plate of syzygy from an old print. some sick magnet action!

after a blend





Here are my first 3 tub trials from using the tribal container. These are multispore. From Syzygy Multispore Grow 2015

4 days after inoculation using my tribal container!:chief:



day 13 i spawned the jars right after that.




3 Tub Grow













Edited by eatyualive (07/13/15 09:52 PM)


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Invisibleeatyualive
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Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive] * 1
    #21927992 - 07/11/15 09:02 AM (8 years, 6 months ago)


Regular mouth half pint jar lid build


about to go ape shit on some liquid inoculants. built a regular mouth 1/2 pint backup for my tribal container.


1/4" guide and 1/2" drill bit


1/4" guide hole


1/2"hole
\

used the 1/2" hole to evenly drill around the hole to widen the hole so that the ship can sit. but loose enough so that when the magnet on the top is used with a glove it can easily be pulled out for a transfer.





Regular mouth Tribal Build


This lid will fit any regular mouth 1/2 pint, pint or quart jar. This comes in really handy if you plan on using your blade assembly without installing it on the bottom of a container. you can pressure cook this lid wrapped in foil at the same time you pressure cook your 1/2 pint, pint or quart jar half full of water. I will be using the 1/2 pint for small Liquid inoculations, the pint jar for larger grain slurry inoculations and the quart jar for pf jar slurries. Each jar has a specific use. This is in addition to the Tribal Container and the Larger Tribal Container.



siliconed the inside ring.

sanded the edge of the lid with sand paper. also added a small bead of silicone to the small suction hole that is built into the cup.

after the silicone was left to set for about 10 minutes. the lid was pressed against the silicone. the silicone was sealed on the inside and outside of the lid.




Taller Higher Volume Container Build


the only downside i see is height of SAB. Also when you get to the end it might be difficult flipping it over 180%. But you can fit twice the volume of LI into it. The lid is somewhat loose. Enough that its easy to push down and open. However, this might be an issue when pressure cooking. I will likely use foil over the lid or micropore tape to hold the lid in place while pressure cooking.

This item was 8$ from walmart. The plastic is sturdy.



1/4" guide hole drilled.


3" hole saw drilled.


trimmed around the edge with an exacto.


sanded down with sand paper.  blade assembly fits perfect.
 

rtv 8" container


testing for any leaks tonight.



tested no leaks




Edited by eatyualive (07/23/15 11:12 PM)


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Invisibleeatyualive
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Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive] * 1
    #21927993 - 07/11/15 09:03 AM (8 years, 6 months ago)


Liquid Inoculant and GS2G(Grain Slurry to Grain Transfer) Below


Now normally I was using a 2:1 grain to water ratio for these grain slurries as liquid inoculant. However, I wanted to try a smaller volume of grain to see how far a quart jar really can be expanded. This example shows how a GLC(Grainwater Liquid Culture) was basically extracted from a spoonful of grain. Once blended the mycelial water was poured into receiving quart grain jars for transfer. None of the grains from the GLC jar were poured into receiving jars this time. My normal GS2G method involves making more of a mud consistency blend and pouring the grains and blended water into the receiving jars.

One spoonful of a colonized quart jar was used per pint of water for the Tribal Slurry(GS2G) illustrated below. 1 plate to 1/4 pint of water was used for the Liquid Inoculant below.

So my GLC and LI jars are almost finished. The LI jars won slightly. I had to shake both because they were taking forever(more than 4 days). Im spoiled by slurry and the 2:1 grain to water slurries.  For pure mycelial water, the colonization was as fast as the LI from a grain jar. 9 days full colonization. This is slow on my end for g2g.

Grain slurry and pf jar slurry are take half the time to colonize jars wothout a shake. The LI and GLC took 9 days with a shake on day 5 to colonize 100%. Although ive compared them several times.  I will still continue to do so in this thread.

Day 1-9: Only 20 quarts were used with LI. 30 used with the GLC. the jars on the right of the colonized quart are a Syzygy clone. Jars on the left are an amazon clone. The quart jars were all shaken on day 5.



GLC Amazon

Day of spawning


LI Syzygy



Liquid Inoculant: 1 pint water to 1 plate agar


A regular mouth quart jar was fitted with a blade assembly and filled with 1 pint of water. The Tribal lid for this jar was wrapped in foil and pressure cooked for 20 minutes at 15psi. The plate was dropped in the quart jar and blended for 2(3-5 second bursts). Taken back into the SAB. Then the lids were switched for pouring.

Normally, I used 1/4 pint of water per plate with the LI. This volume of water was 4 times the normal volume. In this trial the LI showed leap off on the 2nd day. This is Penis Envy.



to be updated...

Quote:



Normal procedure for GS2G transfers. Now incorporating the use of the Tribal Container to make the entire process streamlined, easy and less messy.

SLURRY TYPE 2:

GS2G Transfer(Grain Slurry to Grain jar transfer): wbs grain jar blended and poured into other grain jars.

A ratio of 1 pint grain spawn to 1/2 pint of water was used for 50 quarts of colonized grain.

Do all of this as sterile as possible. Research on sterile technique is assumed prior to this.

1. Fill a pint jar half full of distilled water and place the blade assembly on it. Pressure cook for 20 mins at 15psi. Allow jar to cool.
2. Prepare your clean area(Shmuvbox, SAB, Flowhood) and your substrates and tubs. Setup for spawning.
3. Using a phonebook, break up the grain inside the jar.
4. In your SAB, Take the colonized pint of grain loosen the lid. Loosen the lid of the sterile water pint jar.
5. pour water from water pint into grain pint.
6. place blade assembly lid on grain pint and water pint jar.
7. Blend on your oster blender base. Hold the jar in place with your hand while blending. I blend about 30 seconds.
8. In your SAB, Pour the grain slurry into your quarts jars. You pour by eye about 1 tablespoon volume of slurry per Quart Grain Jar.
9. Wait 3-4 days until full colonization. Give it a day or two more and then your ready to spawn your tubs. You can shake your grain a day before spawning to test for any contamination.

Regular Mouth Lids


Quarts and Pints(Pints will each be used as masters to slurry 100 more quarts each. In the last picture you can see all the colonized pints and 1 quart that i slurried. all the colonized jars are on the last bottom picture. each one of those pints will be used as a master for more than 30 jars at a time)judging from experience, each pint can do at least 200 quart jars of grain. i easily did 30 tonight and had to throw out 2/3rds of the pint jar of grain leftover.





SLURRY VS LIQUID INOCULANT

The Liquid inoculant(texan yellow cap clone-3rd transfer plate) was inoculated on 8-11-2015. The slurry(Thai Lipa Yai multispore pf jar) was inoculated on 8-16-2015. The Liquid inoculant is a fresh clone Texas Yellow Cap plate from agar. The slurry is a 1 years old multispore Thai Lipa Yai from a pf jar that had been sitting in the fridge from 2014.

As you can see the first image was taken on 8-18-2015. 2 days after the slurry was inoculated. The last picture was taken today. So the LI took roughly 8 days to colonize 99%. The slurry in 3 days has almost caught up with the LI. Neither set of jars has been shaken since inoculation.

If you zoom in you can see the tape label. The LI jars are to the left of the LI label. The Slurry jars are to the right of the label. So half the speed on grain. This is consistent for both LI and Slurry with a multitude of tests.

A little background on these cultures. the thai culture is the fastest growing cube culture i have. tex is 2nd fastest. very close in speed. but the thai is multispore and the tex is a clone. the thai pf jar sat in the fridge for over a year and still caught up to the fresh tex clone culture.

check it out. day 4 on the slurry almost done. caught right up to the LI that had a 5 day head start.



Edited by eatyualive (08/20/15 06:21 PM)


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OfflineDarkhome
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Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
    #21928364 - 07/11/15 11:05 AM (8 years, 6 months ago)

You guys are Amazing! New ideas are what make advances in this hobby possible! :bow2:


--------------------
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"NOTHING"...I post or say is "TRUE"...Any pictures are taken from the Web and any conversation is just research for a fictional character. That being said; He is constantly seeking knowledge and evolving.


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InvisiblecArcace-x
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Re: Mycelium Water, A Tribal Container [beta version] [Re: Darkhome]
    #21930439 - 07/11/15 07:24 PM (8 years, 6 months ago)

Quote:

Darkhome said:
You guys are Amazing! New ideas are what make advances in this hobby possible! :bow2:




No YoU are amazing!!!

haha thanks mate!


eatyualive... You tha man!!!
:rocketcrotch:

The video of the inoculation in the OP been done 2 days ago and its showing good recovery.
I will update the grow to the OP as things progress.
Other member's built and other options/ideas will also be added to the OP as things move on

cheers
:toast:
:chief:


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Invisibleeatyualive
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Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
    #21933034 - 07/12/15 11:25 AM (8 years, 6 months ago)

look what i found. I think this is the one Dhearic found. I will give it a try. It's huge she said.



the only downside i see is height of SAB. Also when you get to the end it might be difficult flipping it over 180%. But you can fit twice the volume of LI into it. The lid is somewhat loose. Enough that its easy to push down and open. However, this might be an issue when pressure cooking. I will likely use foil over the lid or micropore tape to hold the lid in place while pressure cooking.

This item was 8% from walmart. The plastic is sturdy.


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Edited by eatyualive (07/12/15 11:34 AM)


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InvisiblecArcace-x
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Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
    #21934536 - 07/12/15 06:05 PM (8 years, 6 months ago)

:rockon:
keep it updated man!!

almost 48hrs after inoculation:


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Invisibleeatyualive
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Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
    #21934890 - 07/12/15 07:55 PM (8 years, 6 months ago)



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Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
    #21934915 - 07/12/15 08:02 PM (8 years, 6 months ago)

That's the most serendipitous use of a "blender bottle" I've ever seen.


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Re: Mycelium Water, A Tribal Container [beta version] [Re: OPB]
    #21939415 - 07/13/15 09:31 PM (8 years, 6 months ago)



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Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
    #21940001 - 07/13/15 11:44 PM (8 years, 6 months ago)

What's the point of the magnets? Is it so you can take the plug out with a single finger? Any other use?


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Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
    #21940082 - 07/14/15 12:04 AM (8 years, 6 months ago)

 single finger


--------------------


A cube is NOT a cube.

FALL IN LOVE WITH LC
FOTTSE!!!
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InvisiblecArcace-x
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Re: Mycelium Water, A Tribal Container [beta version] [Re: azur]
    #21940507 - 07/14/15 03:25 AM (8 years, 6 months ago)

:whathesaid:

one can also do a 2 finger thing if interested


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Invisibleeatyualive
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Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
    #21940689 - 07/14/15 05:32 AM (8 years, 6 months ago)



testing for any leaks tonight.


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Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive] * 2
    #21940728 - 07/14/15 05:54 AM (8 years, 6 months ago)

i really like the lid, look easy to pour with it too
i though about something like that at the very beginning but i havent found any good pp5 bottle like that at the time so i went for the jar
but havent looked at walmart..

found the original plan... lol
i though it wouldn't be good for slurries but just for LI.... until i tried! :tongue:


thats some microsoft paint skillz! hahah :lol:


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Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x] * 1
    #21944420 - 07/14/15 10:52 PM (8 years, 6 months ago)





Doesn't leak and blends myc like a charm :thumbup:


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Re: Mycelium Water, A Tribal Container [beta version] [Re: Dhearic]
    #21945891 - 07/15/15 05:26 AM (8 years, 6 months ago)

:mindblown:

Me want this model!!!!!

Rock-on Dhearic!!! :rockon:


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Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
    #21946120 - 07/15/15 07:16 AM (8 years, 6 months ago)

I'll take 2. Where do I send my $12.95 + $4.99 S/H ?


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Re: Mycelium Water, A Tribal Container [beta version] [Re: Dhearic]
    #21956930 - 07/17/15 04:44 PM (8 years, 6 months ago)

Quote:

Dhearic said:




Doesn't leak and blends myc like a charm :thumbup:



nice! im going to build a small one soon also. im testing both this weekend. i should have at least 50 quarts ready by then!

time to get to work. ill update the op post i have with the jars colonizing once it becomes available.


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