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cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
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Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#22118246 - 08/20/15 07:31 PM (8 years, 5 months ago) |
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furious eatyualive!
update of the grow... one of the tub got caugh trich.. I removed it and recovered well

the other one went fine, had 2 flush, the second only few fruits... never rehydrated it because I was too buzy and didn't had enough fuck to give 1st flush

and some fruits of the second flush a bit bigger 
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
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Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#22118296 - 08/20/15 07:46 PM (8 years, 5 months ago) |
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  badass carc.

Quote:
maddchef said: The slurry I did did OK. I would got close to the same results from regular g2g. Split a pint of grain between 10 bags
grain slurry? how long did it take and what size bags of grain were you using?
yeah its slower than a pf jar slurry. its more on the speed of a liquid inoculant with a plate and GLC. but you can use that same pint of grain to inoculate at least 100 quarts! thats the beauty of it. where if i g2ged that pint id get maybe 10 that would take longer than a week to colonize with a shake.
Edited by eatyualive (08/20/15 07:53 PM)
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
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Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22123345 - 08/21/15 04:30 PM (8 years, 5 months ago) |
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final update.
The Liquid inoculant(texan yellow cap clone-3rd transfer plate) was inoculated on 8-11-2015. The slurry(Thai Lipa Yai multispore pf jar) was inoculated on 8-16-2015. The Liquid inoculant is a fresh clone Texas Yellow Cap plate from agar. The slurry is a 1 years old multispore Thai Lipa Yai from a pf jar that had been sitting in the fridge from 2014.
As you can see the first image was taken on 8-18-2015. 2 days after the slurry was inoculated. The last picture was taken today. So the LI took roughly 8 days to colonize 99%. The slurry in 3 days has almost caught up with the LI. Neither set of jars has been shaken since inoculation.
If you zoom in you can see the tape label. The LI jars are to the left of the LI label. The Slurry jars are to the right of the label. So half the speed on grain. This is consistent for both LI and Slurry with a multitude of tests.
A little background on these cultures. the thai culture is the fastest growing cube culture i have. tex is 2nd fastest. very close in speed. but the thai is multispore and the tex is a clone. the thai pf jar sat in the fridge for over a year and still caught up to the fresh tex clone culture.
check it out. day 4 on the slurry almost done. caught right up to the LI that had a 5 day head start.
    
Here are some of the LI and Slurry jars that were in the back pulled to the front. As you can see, the LI jars are not 100%. Most of them are. But there are a few that are not. The slurry jars on the other hand, are all 99% or finished.
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maddchef
Vaginal escape artist



Registered: 09/04/09
Posts: 5,602
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Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22123867 - 08/21/15 06:34 PM (8 years, 5 months ago) |
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Yeah it was grain slurry to 4qt bags. I believe I made it too thick for my liking though so technically it did 40qts since it was split between 10 bags.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
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Re: Mycelium Water, A Tribal Container [beta version] [Re: maddchef]
#22124010 - 08/21/15 07:18 PM (8 years, 5 months ago) |
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ok. Yeah maybe you needed to mix it up a little more. i usually shake each jar for about 20-30 seconds. so if its a big bag like that, you may need to move it around to get the slurry everywhere a little more.
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maddchef
Vaginal escape artist



Registered: 09/04/09
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Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22125199 - 08/22/15 12:20 AM (8 years, 5 months ago) |
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I may do it again sometime soon but for now imma stop fucking with blenders, pouring, mixing, chopping, slicing, dicing etc.
Gonna dive back into lc's for a little while but let it be known that your numbers hold up.
-------------------- In the land of the blind, the one eyed man is king. All mushrooms are edible, but some only once..... Easier than cakes I do science and shit.
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The Dom
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Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22514698 - 11/12/15 04:02 PM (8 years, 2 months ago) |
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Quote:
eatyualive said: look what i found. I think this is the one Dhearic found. I will give it a try. It's huge she said.
 
the only downside i see is height of SAB. Also when you get to the end it might be difficult flipping it over 180%. But you can fit twice the volume of LI into it. The lid is somewhat loose. Enough that its easy to push down and open. However, this might be an issue when pressure cooking. I will likely use foil over the lid or micropore tape to hold the lid in place while pressure cooking.
This item was 8% from walmart. The plastic is sturdy.
Sorry for bringing this back but i thought it would be good to have clarification in-thread. We dont need to add anything to the lid for the blender bottle except SHIP(if we want) and syringe filter(if we want) because it already has the pour spout? and was the clear you used 100% silicone? Ive got some now and I'd rather make another trip to the store if what I have now wont hold up
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
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Re: Mycelium Water, A Tribal Container [beta version] [Re: The Dom]
#22514841 - 11/12/15 04:47 PM (8 years, 2 months ago) |
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if you only intend to blend and pour. then no for that blender bottle. . you would attach the bottom blade assembly and seal that to the blender jar. if you wanted to store the liquid inoculant, then it might be beneficial to add a ship.
i place a piece of foil over the lid to keep it sealed during pressure cook cycles. i only use it to blend and immediately pour.
it makes a great amount of liquid inoculant for use.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
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Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22514950 - 11/12/15 05:18 PM (8 years, 2 months ago) |
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a few tubs done with cornmeal plate liquid inoculation using the tribal container!
   
         
 
Edited by eatyualive (12/09/15 08:59 PM)
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The Dom
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Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22517492 - 11/13/15 10:22 AM (8 years, 2 months ago) |
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nice pics, ive been reading through a lot of your posts lately eatyualive, good stuff! thanks for the clarification
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The Dom
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Re: Mycelium Water, A Tribal Container [beta version] [Re: The Dom]
#22522249 - 11/14/15 12:38 PM (8 years, 2 months ago) |
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so wait are your blender bottle tops PP5 also? my base is but my lid is HDPE, this will melt in pc wont it?
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
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Re: Mycelium Water, A Tribal Container [beta version] [Re: The Dom]
#22522297 - 11/14/15 12:46 PM (8 years, 2 months ago) |
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yes you want pp5. im not sure about the hdpe. but i wouldn't risk it.
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The Dom
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Re: Mycelium Water, A Tribal Container [beta version] [Re: Dhearic]
#22526020 - 11/15/15 01:29 AM (8 years, 2 months ago) |
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Quote:
Dhearic said:


Doesn't leak and blends myc like a charm 
Would either you or eatyualive mind checking if your lid is made of something else? After posting I found somewhere that the melting pt varies but is around 260f so it seems possible it may survive pc cycle, if i dont get a definitive answer back I'll pc it in an autoclave bag so I dont make a mess. The rest of my container is pp5 btw its the same kind as the one in the pic just a diff color.
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
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Re: Mycelium Water, A Tribal Container [beta version] [Re: The Dom]
#22526041 - 11/15/15 01:44 AM (8 years, 2 months ago) |
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my black lid has a 2 on it. and ive pced it many times for 20 minutes at 15psi.
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cArcace-x
YeAh!!



Registered: 10/17/13
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Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22526526 - 11/15/15 07:51 AM (8 years, 2 months ago) |
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might be usefull 
Quote:
POLYPROPYLENE (PP)
Translucent Autoclavable Maximum temperature: 135°C (275°F) Brittleness temperature: 0°C (32°F)
Polypropylene has relatively poor impact strength, but excellent compatibility with weak and concentrated acids, bases and alcohol.* Polypropylene is commonly used to make bottles, vials, pumps, funnels and clothing.
POLYETHYLENE (PE)
Translucent Not autoclavable Maximum temperature for high-density polyethylene (HDPE) is 120°C (248°F) Maximum temperature for low-density polyethylene (LDPE) is 80°C (176°F) Brittleness temperature for both HDPE and LDPE is -100°C (-100°F)
Polyethylene has high impact strength and excellent compatibility with weak and concentrated acids, bases and alcohol.* Polyethylene is used to manufacture waste bags, bottles, refuse containers, pumps and secondary containment platforms.
POLYCARBONATE (PC)
Transparent Autoclavable Maximum temperature: 135°C (275°F) Brittleness temperature: -135°C (-135°F)
Polycarbonate has high impact strength and excellent compatibility with weak acids.* Polycarbonate is commonly used to make non-breakable windows, safety eyewear and face shields.
POLYMETHYLPENTENE (PMP)
Transparent Autoclavable Maximum temperature: 175°C (347°F) Brittleness temperature: 20°C (68°F)
Polymethylpentene has excellent compatibility with weak and concentrated acids, bases and alcohol.* Polymethylpentene is commonly used to make beakers and graduated cylinders.
PTFE Resin
Opaque Autoclavable Maximum temperature: 260°C (50°F) Brittleness temperature: -100°C (-100°F)
PTFE resin has excellent compatibility with almost all chemicals.* PTFE resin is used to produce pumps, stoppers, tubing, containers, non-stick pans and samplers.
POLYVINYL CHLORIDE (PVC or Vinyl)
Clear Not autoclavable Maximum temperature: 70°C (158°F) Brittleness temperature: -30°C (-22°F)
Polyvinyl chloride has excellent compatibility with almost all chemicals.* Polyvinyl chloride is used to manufacture plumbing pipes, raincoats, footwear, earplugs, pumps and tubing.
POLYETHYLENE TEREPHTHALATE COPOLYMER (PET)
Clear Not autoclavable Maximum temperature: 70°C (158°F) Brittleness temperature: -40°C (-40°F)
Polyethylene terephthalate copolymer has excellent compatibility with weak acids, alcohol and aliphatic hydrocarbons.* It is used to make face shields and bottles.
POLYSTYRENE (PS)
Clear Not autoclavable Maximum temperature: 90°C (194°F) Brittleness temperature: -20°C (-4°F)
Polystyrene has high strength and impact resistance and excellent compatibility with weak acids, alcohol and bases.* Ice buckets and scoops are commonly made with polystyrene.
POLYMETHYL METHACRYLATE (PMMA or Acrylic)
Clear Autoclavable Maximum temperature: 50°C (122°F) Brittleness temperature: 20°C (68°F)
Polymethyl methacrylate does not have excellent compatibility with any group of chemicals.* Bench top shields and machine guards are made from polymethyl methacrylate.
*Polymers described as having excellent compatibility are generally capable of withstanding damage from the chemicals named for 30 days of constant exposure. This is only a general indication. Always check chemical compatibility with each chemical for the containers used for them.
The following plastics chart supplied by Nalgene, includes all of the plastics mentioned in this document. This chart gives many useful ratings pertaining to these plastics including temperature tolerances, permeability and sterilization processes.
http://www.grainger.com/content/qt-types-of-plastics-213
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The Dom
Stranger
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Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#22536285 - 11/17/15 07:29 AM (8 years, 2 months ago) |
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Quote:
eatyualive said: my black lid has a 2 on it. and ive pced it many times for 20 minutes at 15psi.
Thanks for confirming I was not looking forward to the trial run
Quote:
cArcace-x said: might be usefull 
Quote:
POLYPROPYLENE (PP)
Translucent Autoclavable Maximum temperature: 135°C (275°F) Brittleness temperature: 0°C (32°F)
Polypropylene has relatively poor impact strength, but excellent compatibility with weak and concentrated acids, bases and alcohol.* Polypropylene is commonly used to make bottles, vials, pumps, funnels and clothing.
POLYETHYLENE (PE)
Translucent Not autoclavable Maximum temperature for high-density polyethylene (HDPE) is 120°C (248°F) Maximum temperature for low-density polyethylene (LDPE) is 80°C (176°F) Brittleness temperature for both HDPE and LDPE is -100°C (-100°F)
Polyethylene has high impact strength and excellent compatibility with weak and concentrated acids, bases and alcohol.* Polyethylene is used to manufacture waste bags, bottles, refuse containers, pumps and secondary containment platforms.
POLYCARBONATE (PC)
Transparent Autoclavable Maximum temperature: 135°C (275°F) Brittleness temperature: -135°C (-135°F)
Polycarbonate has high impact strength and excellent compatibility with weak acids.* Polycarbonate is commonly used to make non-breakable windows, safety eyewear and face shields.
POLYMETHYLPENTENE (PMP)
Transparent Autoclavable Maximum temperature: 175°C (347°F) Brittleness temperature: 20°C (68°F)
Polymethylpentene has excellent compatibility with weak and concentrated acids, bases and alcohol.* Polymethylpentene is commonly used to make beakers and graduated cylinders.
PTFE Resin
Opaque Autoclavable Maximum temperature: 260°C (50°F) Brittleness temperature: -100°C (-100°F)
PTFE resin has excellent compatibility with almost all chemicals.* PTFE resin is used to produce pumps, stoppers, tubing, containers, non-stick pans and samplers.
POLYVINYL CHLORIDE (PVC or Vinyl)
Clear Not autoclavable Maximum temperature: 70°C (158°F) Brittleness temperature: -30°C (-22°F)
Polyvinyl chloride has excellent compatibility with almost all chemicals.* Polyvinyl chloride is used to manufacture plumbing pipes, raincoats, footwear, earplugs, pumps and tubing.
POLYETHYLENE TEREPHTHALATE COPOLYMER (PET)
Clear Not autoclavable Maximum temperature: 70°C (158°F) Brittleness temperature: -40°C (-40°F)
Polyethylene terephthalate copolymer has excellent compatibility with weak acids, alcohol and aliphatic hydrocarbons.* It is used to make face shields and bottles.
POLYSTYRENE (PS)
Clear Not autoclavable Maximum temperature: 90°C (194°F) Brittleness temperature: -20°C (-4°F)
Polystyrene has high strength and impact resistance and excellent compatibility with weak acids, alcohol and bases.* Ice buckets and scoops are commonly made with polystyrene.
POLYMETHYL METHACRYLATE (PMMA or Acrylic)
Clear Autoclavable Maximum temperature: 50°C (122°F) Brittleness temperature: 20°C (68°F)
Polymethyl methacrylate does not have excellent compatibility with any group of chemicals.* Bench top shields and machine guards are made from polymethyl methacrylate.
*Polymers described as having excellent compatibility are generally capable of withstanding damage from the chemicals named for 30 days of constant exposure. This is only a general indication. Always check chemical compatibility with each chemical for the containers used for them.
The following plastics chart supplied by Nalgene, includes all of the plastics mentioned in this document. This chart gives many useful ratings pertaining to these plastics including temperature tolerances, permeability and sterilization processes.
http://www.grainger.com/content/qt-types-of-plastics-213
Thank you but I found those and other similar temp ratings for hdpe suggesting it would not survive the pc but firsthand experience from eatyualive was exactly what I needed
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cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
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Re: Mycelium Water, A Tribal Container [beta version] [Re: The Dom]
#22538834 - 11/17/15 07:08 PM (8 years, 2 months ago) |
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cool, i was just posting beceause it can be usefull
post result if you want
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eatyualive
Eat's You Alive :)



Registered: 08/17/01
Posts: 19,026
Loc: In Your Head
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Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#22578046 - 11/26/15 12:04 PM (8 years, 2 months ago) |
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ive finally updated my tribal lid to have the magnets.
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cArcace-x
YeAh!!



Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
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Re: Mycelium Water, A Tribal Container [beta version] [Re: eatyualive]
#22603033 - 12/02/15 07:18 AM (8 years, 1 month ago) |
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i'm waiting for diamond bits............
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Juiceh
Dabbing All Day



Registered: 09/25/12
Posts: 3,208
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Re: Mycelium Water, A Tribal Container [beta version] [Re: cArcace-x]
#22709048 - 12/28/15 12:54 PM (8 years, 1 month ago) |
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Anyone use the oster milkshake blender blades to make their LI?
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