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Djchrist
Student

Registered: 06/06/11
Posts: 618
Loc: South Africa

Last seen: 9 months, 14 days

Re: Stro's Cleaning and Isolating On Agar

[Re: blindingleaf]
#20158049 - 06/20/14 04:30 AM (12 years, 2 months ago)

Bookmarked :thumbup:

:thumbup:

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Cl0ud.9.Warl0ck
Hunter

Registered: 05/17/14
Posts: 484
Loc: Mountains
Last seen: 1 year, 8 months

Re: Stro's Cleaning and Isolating On Agar

[Re: Stropharis]
#20295300 - 07/19/14 07:30 AM (12 years, 1 month ago)

Was A subjected to any further tests to try an isolate that additional strain? Or?
Thanks for the procedure explanation! You have helped this neophyte out a great deal!

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Stropharis

Registered: 04/02/07
Posts: 1,854

Re: Stro's Cleaning and Isolating On Agar

[Re: Cl0ud.9.Warl0ck]
#20336354 - 07/27/14 06:10 AM (12 years, 25 days ago)

Cl0ud.9.Warl0ck said:

Was A subjected to any further tests to try an isolate that additional strain? Or?
Thanks for the procedure explanation! You have helped this neophyte out a great deal!

I honestly don't remember, unlikely, it served as a good learning tool at the time though.

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Current Grows

Stro's Write Ups
The Noob Forum
Amateur Mycologists United
The Tribe

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cronicr

Registered: 08/07/11
Posts: 65,471
Loc: Van Isle

Last seen: 3 months, 27 days

Re: Stro's Cleaning and Isolating On Agar

[Re: Stropharis]
#20336892 - 07/27/14 10:16 AM (12 years, 25 days ago)

Stropharis said:

Cl0ud.9.Warl0ck said:

Was A subjected to any further tests to try an isolate that additional strain? Or?
Thanks for the procedure explanation! You have helped this neophyte out a great deal!

I honestly don't remember, unlikely, it served as a good learning tool at the time though.

sneaky guy logs in like a ninja and doesn't even say hi :mad2:

:mad2:

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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Dilated
KB Cubensis Fanaticus

Registered: 03/28/14
Posts: 2,347
Loc: The Ether
Last seen: 5 years, 9 months

Re: Stro's Cleaning and Isolating On Agar

[Re: cronicr]
#20337632 - 07/27/14 12:52 PM (12 years, 25 days ago)

strictly business :smile:

:smile:

by the way thanks stro, awesome write up

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:1up:

Improved melted SFD/lid tek :1up:

:1up:

Dilated's Ironed Polyfil Filters for Monotubs :1up:

:1up:

:1up:

Dilated's Ironed Polyfil Filters :1up:

:1up:

:1up:

:1up:

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cronicr

Registered: 08/07/11
Posts: 65,471
Loc: Van Isle

Last seen: 3 months, 27 days

Re: Stro's Cleaning and Isolating On Agar

[Re: Dilated]
#20337650 - 07/27/14 12:55 PM (12 years, 25 days ago)

and yet i dub the unforgiven :hehehe:

:hehehe:

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It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

Extras:

Stropharis

Registered: 04/02/07
Posts: 1,854

Re: Stro's Cleaning and Isolating On Agar

[Re: cronicr]
#20338325 - 07/27/14 03:10 PM (12 years, 25 days ago)

Is that a TC tag I see? Well deserved Brother, congrats!

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cronicr

Registered: 08/07/11
Posts: 65,471
Loc: Van Isle

Last seen: 3 months, 27 days

Re: Stro's Cleaning and Isolating On Agar

[Re: Stropharis]
#20338328 - 07/27/14 03:10 PM (12 years, 25 days ago)

thnx buddy! hope life is treating ya well!

--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

Extras:

TheEaglesGift
The Nagual

Registered: 04/10/11
Posts: 10,554
Loc: Ixtlan, Mexico

Re: Stro's Cleaning and Isolating On Agar

[Re: cronicr]
#20343396 - 07/28/14 03:48 PM (12 years, 24 days ago)

This is an absolutely great write up. 5 for you Stropharis.

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AMU (Amateur Mycologists United)

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ant707
not a puppet

Registered: 07/26/14
Posts: 579
Loc: northeast florida

Last seen: 9 years, 6 months

Re: Stro's Cleaning and Isolating On Agar

[Re: TheEaglesGift]
#20408393 - 08/12/14 11:29 AM (12 years, 9 days ago)

thanks a lot for the write up! :seriousthumbsup:

:seriousthumbsup:

i just got agar, lme, and petri dishes in the mail today!

i have a related question that i hope no one minds answering here.....

the petri dishes i got are the polystyrene one time use kind, can i make up a batch of lmea and pour them all at once, then store them till i need them?

also i got both the three section ones (i was hoping to save space while looking for sectoring) and the non divided kind (once i get closer to isolates)
has anyone used these or have any opinions on them?

Extras:

Stropharis

Registered: 04/02/07
Posts: 1,854

Re: Stro's Cleaning and Isolating On Agar

[Re: ant707]
#20409680 - 08/12/14 04:56 PM (12 years, 9 days ago)

ant707 said:

can i make up a batch of lmea and pour them all at once, then store them till i need them?

You shouldn’t have any issues if they are stored airtight in the plastic sleeves that they came in, and at room temperature. As long as they don’t dry out on you, a few months or so, you should be good. I usually just do a sleeve at a time or pour as needed.

Quote:

ant707 said: i got both the three section ones (i was hoping to save space while looking for sectoring) and the non divided kind (once i get closer to isolates)has anyone used these or have any opinions on them?

I have used the sectioned plates before and I do prefer them for this type of work, very user friendly.

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The Noob Forum
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ant707
not a puppet

Registered: 07/26/14
Posts: 579
Loc: northeast florida

Last seen: 9 years, 6 months

Re: Stro's Cleaning and Isolating On Agar

[Re: Stropharis]
#20410189 - 08/12/14 07:01 PM (12 years, 8 days ago)

Quote:

Stropharis said:
You shouldn’t have any issues if they are stored airtight in the plastic sleeves that they came in, and at room temperature. As long as they don’t dry out on you, a few months or so, you should be good.

how would i go about storing them "airtight" in the sleeve they came in? vacuum seal the sleeve with a foodsaver or sumthin?

or, do you mean each individual dish airtight? like parafilm or plastic wrap?

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Stropharis

Registered: 04/02/07
Posts: 1,854

Re: Stro's Cleaning and Isolating On Agar

[Re: ant707]
#20410250 - 08/12/14 07:17 PM (12 years, 8 days ago)

I keep the open end of the plastic folder over, tapped shut, and the remaining unused sleeve of dishes in a small clean tupperware like container.

--------------------
Current Grows

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The Tribe

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ant707
not a puppet

Registered: 07/26/14
Posts: 579
Loc: northeast florida

Last seen: 9 years, 6 months

Re: Stro's Cleaning and Isolating On Agar

[Re: Stropharis]
#20410445 - 08/12/14 08:02 PM (12 years, 8 days ago)

:nodofunderstanding:

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jordano
Stranger

Registered: 08/06/14
Posts: 55
Last seen: 11 years, 7 months

Re: Stro's Cleaning and Isolating On Agar

[Re: Stropharis]
#20581059 - 09/17/14 07:57 PM (11 years, 10 months ago)

Amazing write up, thank you so much for all ur help stro. :thumbup:

:thumbup:

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pablokabute
Hari ng Amag

Registered: 11/22/11
Posts: 5,280
Loc: rural ghetto

Re: Stro's Cleaning and Isolating On Agar

[Re: jordano]
#20582792 - 09/18/14 08:57 AM (11 years, 10 months ago)

Wished I found this since this writeup was first conceived...

Thanks Stropharia... For your big heart and brilliance.

:getstoned:

:getstoned:

Just awesoooooooome...
Here we goooo!!!

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Fermented Mushrooms!!
--- https://www.shroomery.org/forums/showflat.php/Number/23378638/fpart/1/vc/1

'The second seal: “All CONTAMINATED things and events are unsatisfactory.”'

"I envy you. You North Americans are very lucky. You are fighting the most important fight of all - you live in THE HEART OF THE BEAST."

--Anonymous Guerilla, or is he..

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Stropharis

Registered: 04/02/07
Posts: 1,854

Re: Stro's Cleaning and Isolating On Agar

[Re: pablokabute]
#20589513 - 09/19/14 05:24 PM (11 years, 10 months ago)

:raisemyglass:

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germish
Stranger

Registered: 11/12/13
Posts: 377
Loc: PNW WA

Last seen: 5 years, 1 month

Re: Stro's Cleaning and Isolating On Agar

[Re: Stropharis]
#20657997 - 10/04/14 03:39 PM (11 years, 10 months ago)

This jar of Azures is like 4 months old now it has been in the fridge foil cap on always. It is alive but in need of a transfer. I know the pic is crap but its the best I can get.

Do I remove a piece from the edge or, should I remove a piece that is bluing so I can be sure it is an active isolate? Also, more info on the jar, it got one drop of MS and appeared to be a single growth point. On potatoe water agar. Thanks

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cronicr

Registered: 08/07/11
Posts: 65,471
Loc: Van Isle

Last seen: 3 months, 27 days

Re: Stro's Cleaning and Isolating On Agar

[Re: germish]
#20658012 - 10/04/14 03:45 PM (11 years, 10 months ago)

always try for the leading edge, the bluing is it drying out/degrading

--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor
I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

Extras:

Stropharis

Registered: 04/02/07
Posts: 1,854

Re: Stro's Cleaning and Isolating On Agar

[Re: germish]
#20659208 - 10/04/14 10:03 PM (11 years, 10 months ago)

This is exactly why I leave it to nicer people like Cron to help out. :suicide:

:suicide:

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Current Grows

Stro's Write Ups
The Noob Forum
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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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