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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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grain water agar? * * 2
    #19612873 -

:crondance:

Edited by cronicr (01/02/16 06:52 PM)

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Re: grain water agar? [Re: MudaFuka] * 1
    #19612875 -

:popcorn:


--------------------
                 
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT!
A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD,
SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List,
EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE
*** *** AFGHAN KUSH GROW LOG *** ***

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Re: grain water agar? [Re: PussyFart] * 1
    #19612929 -

:whathesaid:
i don't bother, i like to limit nutrition for the most part


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: grain water agar? [Re: cronicr] * 2
    #19613117 -

I have used it for agar and LC, diluted and full strength.  Works as well as my MEA or PDA did. If using in a LC I would recommend 1 part grain soak water to 4 parts tapwater or else it's too dark to see through. For agar I usually cut it 50/50.

I have not used it with WBS soak water however, but for wheat,  rye, millet and oats it did fine.


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:headbang3: Pastywhyte's Easy Writeups :headbang3: My Favorite Grow Logs :headbang3:
:headbang3: Avoiding the dreaded facepalm in cultivation :headbang3: The worst threads all have the same subjects :headbang3:
:headbang3: Q & A.M.U. :headbang3:

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Re: grain water agar? [Re: Pastywhyte] * 1
    #19613134 -

i JUST got plain old agar the other day, but have used grain water in those pre made powder MEA's from a vendor.  250ml tap, 250 ml rye soak water (including the coffee, no gypsum tho).  No difference in growth that i can tell.  maybe with 100% grain water it would be different?


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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Re: grain water agar? [Re: Pastywhyte] * 1
    #19613167 -

Pastywhyte said:
I have used it for agar and LC, diluted and full strength.  Works as well as my MEA or PDA did. If using in a LC I would recommend 1 part grain soak water to 4 parts tapwater or else it's too dark to see through. For agar I usually cut it 50/50.

I have not used it with WBS soak water however, but for wheat,  rye, millet and oats it did fine.


AS soon as my wife gets home with my PP5s and agar powder I'm going to make a batch with the water from a mix of millet, wheat and RGS. Do you add anything else or just 50/50 diluted grain water and agar.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

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Re: grain water agar? [Re: MudaFuka] * 1
    #19613238 -

I do 25ml of grain water and .5g agar per dish and I have had good results with it. When I tried PDA and GWA next to each other the GWA germinated a couple days sooner and grew faster. I dont have any experience with MEA

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Re: grain water agar? [Re: MudaFuka] * 1
    #19613318 -

At most I might add a drop of karo but it's not really neccessary.


--------------------
:headbang3: Pastywhyte's Easy Writeups :headbang3: My Favorite Grow Logs :headbang3:
:headbang3: Avoiding the dreaded facepalm in cultivation :headbang3: The worst threads all have the same subjects :headbang3:
:headbang3: Q & A.M.U. :headbang3:

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Re: grain water agar? [Re: Pastywhyte] * 1
    #19613323 -

thanks


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

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Re: grain water agar? [Re: MudaFuka] * 2
    #19629980 -

My agar media journal goes over a number of different recipes :wink:


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Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:

Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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Re: grain water agar? [Re: FrankHorrigan] * 1
    #19630006 -

Thanks Frank. Up to this point agar is the only thing giving me any trouble in this hobby. Its making me a bit mental.


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

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Re: grain water agar? [Re: MudaFuka] * 1
    #19631557 -

g2g is that way with me :oldman2:

i got agar down fine and dandy in the SAB, but g2g is another story.  never once had 100% successful g2g, always get 2-3 receiving jars get con tams.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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Re: grain water agar? [Re: blindingleaf] * 1
    #19631612 -

I was amazed how my resistance to agar work melted away when I tried it. It's pretty straightforward :shrug: even in my "SAB" which is a 66 quart tub upside down with the body pulled away a bit from the lid to allow my clumsy shaking hands in. Haven't needed to do g2g yet though :thumbup:


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Re: grain water agar? [Re: Skinty] * 1
    #19631785 -

im gonna try going from pint to quart in the SAB for g2g.  may have better success.  i use 66 qt also, but not tall enough for the angle to pour grains quart to quart.  that and I'm an idot i guess lol


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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Re: grain water agar? [Re: blindingleaf] * 2
    #19631952 -

Get the tallest, widest tub you can find for your SAB, even I would be pissed trying to G2G in a 66qt.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:

Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU

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Re: grain water agar? [Re: FrankHorrigan] * 1
    #19631961 -

lol.  i just may have to do that, and keep the 66qt for agar, cause its working well for that.  what i have been doing is just putting a wedge from same plate to six grain jars if I'm doing a mono.  take a while tho :frown:


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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Re: grain water agar? [Re: blindingleaf] * 1
    #19636370 -

I recently made two batches of GWA. In the first I used 5 grams of agar powder for each cup of water. In the second I used 10 grams per cup. Nether batch set up properly. the first stayed completely liquid and the second was still way to soft.
I've never had this problem with PDA so I'm wondering if there is something from one of my grains reacting with the agar. the grains I used were millet, wheat and RGS.

Any ideas?


--------------------
AMU
Bottle Tek
Liquid Inoculant Tek                                   
                     

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Re: grain water agar? [Re: MudaFuka] * 1
    #19636401 -

weird.  i dunno.  i have yet to do "homemade" agar.  I'm still trying to use up the pre made mea mix i got.  with that i just sub in 250ml grain soak water (rye) with 250ml tap, and 25 g powder.


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts

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Re: grain water agar? [Re: MudaFuka]
    #29074395 -

It could that you didn't use enough agar , it recommended to use 12g approximately for 1L

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Re: grain water agar? [Re: cuboard] * 2
    #29074408 -

Posters dead fam


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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