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InvisibleFrankHorrigan
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Frank's agar media journal * 25
    #18676888 - 08/08/13 10:13 AM (8 years, 10 months ago)



I'm going to keep track of agar recipes here.

I'll be trying out a whole bunch of different recipes from Aloha medicinals' cookbook.

I'm looking to use most of these for cubensis as well as gourmets. Some are species-specific though, such as the PAT. All the pictures for this thread will be cubensis cultures.

A more in-depth version of this (across multiple species) will be added to my journal at some point.

If anyone is interested feel free to join in the thread, this is going to be an on-going (and sometimes slow) process but I plan to finish it by the end of this calender year.

This is not going to be an agar tutorial initially though maybe it will become one, please refrain from asking unrelated questions.

My first goal was to measure out the agar recipes and bag them up. So here we go...

Each recipe calls for 500mL of water (tap water used unless otherwise noted).

I'm probably omitting the peptone until I have a better supplier. It is expensive and not readily available for me at this time.


0) Grain-dunk agar experiment: 10g of agar to 500mL of water used to dunk 6 quarts of colonized WBS


Here are comparison plates between isolates on grain-dunk agar and malt-extract with yeast agar. I did not use very good sterile technique so the plates are a bit spotty here or there.  These are simply for a visual comparison, they are not going to grains.



Grain-dunk agar works ok, I would add some dextrose to the recipe if I try it again. Growth was pretty wispy compared to other media.

1) Malt-extract: 10g LME(a) / GLME (b), 10g agar


Pictured here is GLMEA, it works great, pretty much the standard I judge media against.


2) Malt-extract yeast: 10g ELME, 10g agar, 1g yeast
 


Vigorous growth. I use this regularly.


3) Malt-extract with activated carbon: 10g ELME, 10g agar, 1g activated carbon



Didn't notice much difference between standard LMEA. Not recommended.


4) Malt-extract with peptone: 10g LME, 10g agar, 1g peptone


5) Malt-extract with yeast and peptone: 10g LME, 10g agar, 1g yeast, 1g peptone


6) Malt-extract with yeast and peptone and activated carbon: 10g LME, 10g agar, 1g yeast, 1g peptone, 1g activated carbon



7) Malt-extract with millet: 10g LME, 10g agar, 10g millet flour



Growth was slower and fuzzier than I'd like. No reason to recommend.


8) 597: 10g agar, 3.75g LME, 0.3g yeast, 0.5g peptone



9) Malt-extract yeast agar with vitamins: 10g LME, 10g agar, 8g ground crickets, 1g yeast, 0.01g multivitamin



10) Malt-extract yeast agar with elements: 10g LME, 10g agar, 1g yeast, 0.3g CaMgZn


Growth was comparable to standard LMEA, not any reason to recommend adding vitamins to agar for cubes.


11) Blood worm agar: 10g agar, 5g dry blood worms



12) Krill agar: 10g agar, 10g dry krill



13) Fish flake dextrose agar: 10g agar, 3g fish flakes, 10g dextrose



This did ok. Interestingly, one of my cultures really loved the shit out of it (see above) and the rest performed better on LMEA.


14) Meal worm agar: 10g agar, 10g meal worms, 3g dextrose, 1g yeast


As you can see, cubes don't really thrive on MWA. Growth was slow and strange. I would not recommend it.


15) Cat food agar: 10g agar, 10g cat food



16) Bird seed malt agar: 10g agar, 2.5g BRF, 5g WBS, 2.5g LME, 0.5g peptone



17) Millet agar: 10g agar, 10g millet flour, optional: 1g yeast



18) Cornmeal dextrose agar: 10g agar, 25g cornmeal, 3g dextrose



19) BRF Malt-extract agar: 10g agar, 40g BRF, 2g malt, 1g yeast



20) Potato dextrose agar: 10g agar, 10g potato starch, 7g dextrose


21) PAT (cordyceps-specific): 10g agar, 100mL potato broth, 1g KH2PO4, 0.5g MgSO4, 1.5g peptone, 0.5g ammonium citrate, 15g dextrose, .025g thiamine hcl



22) Potato dextrose yeast agar: 10g agar, 10g potato starch, 7g dextrose, 1g yeast



Another de facto standard for media. This is my favorite recipe at the moment.


:cheers:


--------------------

Yes, you can bump my old threads with a question.
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You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


Edited by FrankHorrigan (02/07/14 07:36 PM)


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OfflineSylus
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Re: Frank's agar adventures journal [Re: FrankHorrigan]
    #18676894 - 08/08/13 10:15 AM (8 years, 10 months ago)

was reading this in your journal which looked much similar, does the recipe book your taking from call for dry cat food?

sorry just wondering..

EDIT: #15


Edited by Sylus (08/08/13 10:16 AM)


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InvisibleFrankHorrigan
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Re: Frank's agar adventures journal [Re: Sylus]
    #18676898 - 08/08/13 10:17 AM (8 years, 10 months ago)

I am using dry cat food for it.

It will dissolve into a powder when hydrated.

I don't think wet food would have the right consistency, with the chunks and what not :shrug:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Invisibletbagtag
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Re: Frank's agar adventures journal [Re: Sylus]
    #18676900 - 08/08/13 10:17 AM (8 years, 10 months ago)

:pedrophile:


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InvisibleFrankHorrigan
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Re: Frank's agar adventures journal [Re: tbagtag]
    #18676909 - 08/08/13 10:21 AM (8 years, 10 months ago)

:lurker:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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Invisibletbagtag
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Re: Frank's agar adventures journal [Re: FrankHorrigan]
    #18677067 - 08/08/13 11:17 AM (8 years, 10 months ago)

Lol, I have nothing to add just wanted to keep this thread saved for more study.  Tomorrow marks one week since my second agar attempt.  Struggling like a mother fucker with it.


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Offlinejonsnow
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Re: Frank's agar adventures journal [Re: tbagtag]
    #18677113 - 08/08/13 11:34 AM (8 years, 10 months ago)

are you using brewer's yeast or nutritional yeast?


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InvisibleJuiceh
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Re: Frank's agar adventures journal [Re: jonsnow]
    #18677311 - 08/08/13 12:28 PM (8 years, 10 months ago)

:rockon:  :takingnotes:
I Love Agar! I've only worked with MEA thus far. Was thinking of switching it up a little for the next batch. I can't wait to see your results!


--------------------
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How I make 15 lbs of casing in a 941. How I line 12 shoeboxes with 1 39gal bag. How I Deep Fry Cubes!
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InvisibleVirginiaGentleman
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Re: Frank's agar adventures journal [Re: FrankHorrigan]
    #18677612 - 08/08/13 01:49 PM (8 years, 10 months ago)

Quote:

FrankHorrigan said:


1ab) Malt-extract: 10g LME(a) / GLME (b), 10g agar

23) Wood-lovers agar: add 10g sawdust to agar recipe.






Been using (1ab), sometimes going .80LME:1agar with good results

Curious if the sawdust would suspend or settle for (23) though, seems like that would require a pour method which i'm not too fond of.. Maybe i'll give it a shot during the next transfer :takingnotes:

From what i can gather, newspaper pulp or egg carton pulp should have similar effect as sawdust by providing the lignin (i'm assuming) woodies are hungry for.


--------------------
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Edited by VirginiaGentleman (08/08/13 01:51 PM)


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OfflinePhosCap
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Re: Frank's agar adventures journal [Re: FrankHorrigan]
    #18677635 - 08/08/13 01:53 PM (8 years, 10 months ago)

:popcorn:


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InvisibleFrankHorrigan
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Re: Frank's agar adventures journal [Re: jonsnow]
    #18678367 - 08/08/13 04:08 PM (8 years, 10 months ago)

Quote:

VirginiaGentleman said:

Been using (1ab), sometimes going .80LME:1agar with good results

Curious if the sawdust would suspend or settle for (23) though, seems like that would require a pour method which i'm not too fond of.. Maybe i'll give it a shot during the next transfer :takingnotes:

From what i can gather, newspaper pulp or egg carton pulp should have similar effect as sawdust by providing the lignin (i'm assuming) woodies are hungry for.




Good stuff. I know that with most of these recipes I will need to swirl the agar to mix it every few plates poured. I've also heard water used to soak woodchips is good for making agar for wood lovers.

My lions mane is picky and slow on my MEA. I'm going to test this out soon.

Quote:

jonsnow said:
are you using brewer's yeast or nutritional yeast?




Nutritional :thumbup:


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


Edited by FrankHorrigan (08/08/13 10:01 PM)


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InvisibleZarotti
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Re: Frank's agar adventures journal [Re: FrankHorrigan]
    #18678690 - 08/08/13 05:13 PM (8 years, 10 months ago)

Is it true that the lower the nutrition value of the agar&malt mix
the more rhyzomorphic mycelium growth will appear?

And whats the difference inbetween brewer's yeast and nutritional yeast?
Does it matter?


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InvisibleLustyLocks
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Re: Frank's agar adventures journal [Re: Zarotti]
    #18678784 - 08/08/13 05:34 PM (8 years, 10 months ago)

Cat food. Meow!! :shockedpussy:
I might have to try that. It sounds interesting. I imagine there is yeast and other goodies in there?

. I'm looking forward to the upcoming posts in this thread.


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OfflineStromriderM
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Re: Frank's agar adventures journal [Re: LustyLocks]
    #18678899 - 08/08/13 05:55 PM (8 years, 10 months ago)

:takingnotes:


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Offlinejustarandom
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Re: Frank's agar adventures journal [Re: Stromrider]
    #18680546 - 08/08/13 11:37 PM (8 years, 10 months ago)

Quote:

psillyshroomer said:
:takingnotes:



agreed


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Invisiblenucleophile
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Registered: 04/28/13
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Re: Frank's agar adventures journal [Re: justarandom]
    #18680776 - 08/09/13 12:26 AM (8 years, 10 months ago)

I'm curious to see how the cat food agar performs... I grew some oysters on a  cat food/sawdust/grain substrate once... they didn't much care for it but dutifully fruited as oysters are bound to do.


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Offlinepsilocybebonsai
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Re: Frank's agar adventures journal [Re: nucleophile]
    #18681391 - 08/09/13 04:17 AM (8 years, 10 months ago)

commenting for two reasons!

1. so i can come back and see what people are saying and;
2. is there a list as to the benefits of specific nute additives? (i understand sawdust for wood lovers, etc) but a more in depth so i can potentially experiment with my own would be great. for example, could i use straw juice with agar?

thanks ya'll


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Offlinepsilocybebonsai
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Re: Frank's agar adventures journal [Re: nucleophile]
    #18681415 - 08/09/13 04:28 AM (8 years, 10 months ago)

Quote:

nucleophile said:
I'm curious to see how the cat food agar performs... I grew some oysters on a  cat food/sawdust/grain substrate once... they didn't much care for it but dutifully fruited as oysters are bound to do.




i actually did some catfood agar with and oyster tissue clone and it worked very well. my catfood wasnt as powdery as i was hoping (used a blender, not a coffee grinder) and it performed really well (first agar, but i couldnt have expected anything better, really)


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My small photo collection here
My smaller grow log here
Some tips for taking photos of your progress when seeking help here


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InvisiblePestile

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Re: Frank's agar adventures journal [Re: FrankHorrigan]
    #18681452 - 08/09/13 04:50 AM (8 years, 10 months ago)

Have you got a personal favorite and/or do you use different medias for different strains? If so, which is it and why? :smile:


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InvisiblePastywhyteMDiscord
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Re: Frank's agar adventures journal [Re: Pestile]
    #18681622 - 08/09/13 07:19 AM (8 years, 10 months ago)

:popcorn: :takingnotes:


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