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Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, HEPA Filter, Rye Grain, Vermiculite, Wild Bird Seed

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Invisibleagar
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Mixed Grains - Spawn and/or Substrate:
    #3750725 - 02/08/05 01:31 PM (12 years, 19 days ago)



Mixed Grains - Spawn and/or Substrate:

After soaking until various WBS & grains are pliable & well rinsed:

1. Load jars ? + full (leaving room - for addition of G2G & so they will shake after partial colonization).

2. Add Tyvek & lid (lid has 2 (two) ? inch holes drilled through it.

3. Add ring & tighten until tight, then back off ? turn.

4. Add double (or triple) layer coffee filters, held down by rubber band.

5. PC?ed jar (Min - 60 minutes @ 15 psi), tighten lid (just snug - not super tight) & shake while hot.

Footnotes:

Jar # 2 has 2 (two) ? inch holes, set up for G2G transfers - without taping over any holes.

Jar #3 has 3 (three) holes. Two ? inch & 1 (one) 1/8th inch. 1/8th inch hole is for syringe innoc & tape over.

Jar #5 was actually PC?ed 90 minutes @ 16 / 18 psi in All American PC - Sterilizer .

*"pliable" means you can indent - semi-crush some grains between your finger & fingernail.

* Coffee filters are added - as a simple exterior dust cover & you can write on them. Coffee filters will dry out almost instantly, when remover from a hot PC.

* Coffee filters will not suffice as filters - alone , as they will wick moisture & are to porous.

*In this instance, 10% brown rice & 10% rye was added to the WBS mix & soaked - as added diversified nutrients.

* Soak time varies from 12 to 48 hours, dependent on initial dryness of grains & ambient soak temperature. So long as the grain has not sprouted, they are fine.

*A grain mix of this nature is forgiving as to moisture content, in-so-far as some grains absorb more moisture that others & create a moisture balance of some very wet & some much drier grains.

Milo - sorghum for instance has a very hard exterior shell & absorbs moisture much more slowly than rye & rice. Thus, milo will be harder to indent with a fingernail, while rye & rice you can actually cut in half with you fingernail. The combined moisture content averages out very well. Jars are easy to shake while hot & colonize very rapidly.

* WBS alone makes a good spawn material & can be fruited off of. The addition of rye & brown rice simply makes the mixture more nutritious, if you intend to use it as a substrate - rather than as spawn to horse manure / straw mix, or compost bulk substrate.


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Invisibletripndicular
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Re: Mixed Grains - Spawn and/or Substrate: [Re: agar]
    #3750928 - 02/08/05 02:31 PM (12 years, 19 days ago)

Thanx agar, you are the man. :thumbup:
I think you hurt their brains with all them deffinitions  :wink:


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Any information I give is not intended to aide you in the production of potentialy illegal substances !None of my exp comes from growing illegal varities , so take it as you will .
So with that said here is our mission statement .

Then the priest fell into a trance or swoon,& said unto the Queen of heaven ; Write unto us the ordeals; write unto us the rituals; write unto us the law !


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InvisibleTM
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Re: Mixed Grains - Spawn and/or Substrate: [Re: agar]
    #3750990 - 02/08/05 02:51 PM (12 years, 19 days ago)

Nice!

I would only change one thing...

Your inoculation method leaves a clear path for contams to enter.

They can go through the 1/4 holes, accross the surface of the Tyvek and
through the needle hole.

I do the exact same method, but use just (2) 5/16" holes... Shoot through one
of 'em and seal the needle hole with a drop or two of candle wax. :wink: :thumbup:


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================================================



"Have some congratulatory drugs." - C. Montgomery Burns

I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss. :tongue2:

TM™ :cool:


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InvisibleRoadkill
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Re: Mixed Grains - Spawn and/or Substrate: [Re: TM]
    #3751156 - 02/08/05 03:40 PM (12 years, 19 days ago)

Quote:

TripMeister said:
Nice!

I would only change one thing...

Your inoculation method leaves a clear path for contams to enter.

They can go through the 1/4 holes, accross the surface of the Tyvek and
through the needle hole.

I do the exact same method, but use just (2) 5/16" holes... Shoot through one
of 'em and seal the needle hole with a drop or two of candle wax. :wink: :thumbup:




I use a small round bandaid on one hole...so the jar can breathe.
The other hole I put a dab of silicone on...makes a great injection port that reseals.


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Laterz, Road

Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!


Brainiac said:
PM the names with on there names, that means they have mushrooms for sale.



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InvisibleTM
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Re: Mixed Grains - Spawn and/or Substrate: [Re: Roadkill]
    #3751207 - 02/08/05 03:50 PM (12 years, 19 days ago)

Hey RK!

LTNT :smile:

The jar can breathe even better without the band-aid and I don't have to wait
for silicone to dry or worry that it didn't quite seal.


--------------------
================================================



"Have some congratulatory drugs." - C. Montgomery Burns

I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss. :tongue2:

TM™ :cool:


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Invisibleagar
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Re: Mixed Grains - Spawn and/or Substrate: [Re: TM]
    #3751499 - 02/08/05 05:40 PM (12 years, 19 days ago)

Quote:

TripMeister said:

Your inoculation method leaves a clear path for contams to enter.

They can go through the 1/4 holes, accross the surface of the Tyvek and
through the needle hole.

I do the exact same method, but use just (2) 5/16" holes... Shoot through one of 'em and seal the needle hole with a drop or two of candle wax. :wink: :thumbup:




I don't grasp where :confused:?  I innoc through 1/8th inch hole, then tape over. There are no holes in the tyvek under the opposing 1/4 inch holes.

You have two holes, innoc through one, then seal it.

I have 3 holes, innoc through 1 & seal it.

There is no differance, I can see.


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InvisibleTM
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Re: Mixed Grains - Spawn and/or Substrate: [Re: agar]
    #3751577 - 02/08/05 05:55 PM (12 years, 19 days ago)

I said: "a clear path for contams to enter. They can go through the 1/4 holes, accross the surface of the Tyvek and through the needle hole."

What is sealing the hole in the Tyvek, and what makes you think that offset holes are protection?


--------------------
================================================



"Have some congratulatory drugs." - C. Montgomery Burns

I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss. :tongue2:

TM™ :cool:


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Invisibleagar
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Re: Mixed Grains - Spawn and/or Substrate: [Re: TM]
    #3751637 - 02/08/05 06:07 PM (12 years, 19 days ago)


More examples than I have the patience to upload.


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Invisibleagar
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Re: Mixed Grains - Spawn and/or Substrate: [Re: TM]
    #3753487 - 02/09/05 12:10 AM (12 years, 19 days ago)

Quote:

TripMeister said:
I said: "a clear path for contams to enter. They can go through the 1/4 holes, accross the surface of the Tyvek and through the needle hole." What is sealing the hole in the Tyvek, and what makes you think that offset holes are protection?




This does:

1st the little contam bugger has to get by the household near hepa grade allergen air filters, then the 1st Airostat XC inonizer, then 2nd though the in room hepa filter, then 3rd bore through the steel cabinet, then through 3rd, 4th 5th triple layer of coffee filters, gnaw through them one by one, get to the metal lid, then 6th take a hard left and travel across the metal lid, then 7th take a hard right to get to the tyvk, 8th drop down onto it, 9th turn 180 degree?s & heard for the tiny hole in the tyvek that is covered with tape.

If that is a CLEAR PATH & they get through it, they deserve to.


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InvisibleTM
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Re: Mixed Grains - Spawn and/or Substrate: [Re: agar]
    #3753583 - 02/09/05 12:25 AM (12 years, 18 days ago)

That's fine.

1) You never explained how protected your jars are.

2) Everyone else on the board doesn't have that kind of protection, making it inadvisable to use your method (for them).

3) You have to admit that not having the protection you describe would lead to
disaster for the cultivator using the inoculation/preparation method as you
first described it. (without all of your equipment)

4) Please understand that I have the utmost respect for you as an experienced
mycolgist and was just pointing out that there are precautions that must be
taken to ensure against contamination.

Without your described path having to be followed by a mold spore, your jars
would all contaminate.


--------------------
================================================



"Have some congratulatory drugs." - C. Montgomery Burns

I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss. :tongue2:

TM™ :cool:


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Offlinescatmanrav
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Re: Mixed Grains - Spawn and/or Substrate: [Re: TM]
    #3754040 - 02/09/05 01:56 AM (12 years, 18 days ago)

Quote:

TripMeister said:
Without your described path having to be followed by a mold spore, your jars
would all contaminate.




I think your a little quick to judge that the WOULD contaminate. I started off doing tyvek, lid, ring...I did probably 5 dozen jars like that and besides a contaminated syringe and maybe 4 or 5 other jars, my first ones were all free of contams. They were in a tub in tub covered by a blanket in a closet. Thats pretty common of a incubator for a common shroomerite and its still not the clearest path in for contams... I did it and I know it works just fine.

The only reason I moved the tyvek to the outside is so I can write on it (this issue was relized when all the lids had been written on) and its easier to just put the layer of tyvek outside then adding a coffee filter or peice of tape or something. I dont do bulk, just lots of jars of grain so the simpler the better for myself. Writing on it was really the only plus I saw.

A plus for the tyvek under the lid though, is if your doing grain to grain transfers. That damn rubber seal always gets stuck just enough to be a pain when your knocking up 6 dozen jars. Course then you wouldnt really have a problem with it since no hole in the tyvek right?

Ah well..I thought what I did might give me contam problems when I started...wasnt sure how well those spores could crawl...I found spores dont crawl very well between the lid and tyvek.

Just my experience with it :smile:


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"life is like a drop of rain getting closer and closer to falling into a lake, and then when you hit the lake there is no more rain drop, only the lake."

Growing with bags, start to finish (including my new grain and substrate prep)
Anyone looking to start bulk tubs/mono tubs/shotgun hybrids? Good tubs to use..
How I do grain (old still good tips)
Turn your closet into a fruiting chamber
Casing layer colonization and overlay


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InvisibleTM
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Re: Mixed Grains - Spawn and/or Substrate: [Re: scatmanrav]
    #3754067 - 02/09/05 02:03 AM (12 years, 18 days ago)

IME, just accidentally folding a corner of the Tyvek under the rubber seal
causes enough of a gap to let contams in.

I guess it all depends on your environment.

'nuff said.


--------------------
================================================



"Have some congratulatory drugs." - C. Montgomery Burns

I'll probably always do drugs, so that just contributes to the addiction to The Shroomery... It's a vicious circle of bliss. :tongue2:

TM™ :cool:


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Invisibleagar
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Re: Mixed Grains - Spawn and/or Substrate: [Re: TM]
    #3754310 - 02/09/05 02:57 AM (12 years, 18 days ago)

Quote:

TripMeister said: Without your described path having to be followed by a mold spore, your jars would all contaminate.




Untrue:

Even placed in a used cardboard box, on a dusty attached garage shelf where there is no air filter devices of any sort, the method is as effective as the PF with tinfoil tek, any polyfil tek, 2 holes in tyvek - under or over a lid - inject through 1 & tape it over tek. 95+ % uncontaminated is a great rate.

No doubt hundreds upon hundreds of thousands (if not millions) of jar type containers - world wide have used tinfoil as a barrier, verm as a barrier, polyfill as a filter & tyvek as a filter (2 holes - inject in 1 & tape it over).

The FACT the Shroomery exists & thrives, as do many other simular sites world wide is proof these methods work. If otherwise, we would even be here, nor have this wonderfull site to discuss & debate such issues in.

I will agree, your method is sound. You think mine would lead to complete utter failure & I disagree.

Fine, that is your opinion & you are entitled to it.

I believe it is a waste of my time to debate the issue with you any further.

Peace & pass on the magic as often as possible.


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Amazon Shop for: ½ Pint Jars, Agar, Brown Rice Flour, HEPA Filter, Rye Grain, Vermiculite, Wild Bird Seed

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