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Re: Must Read!! New Research Concludes: Cubes Unexposed to Light are 100 Xs more POTENT!! [Re: koopa_troopa]
    #11669850 -

koopa_troopa said:
I seriously doubt a professional mycologist would submit any testing of fungi based around a multispore crapshot...really??


If they didn't say it...
... they didn't do it. 
Period.

As further evidence how would you account for dim light being worse than bright and no light?  Doesn't make sense.

And what's with that chromo machine of theirs, not being able to measure PC with most common solvents?  Cheap or busted.

Sorry to rain... remember I was amongst the most open to this in the beginning.

PS to Doc_T: Yes, their English is flawless, isn't it?  Hmm...
(Nah)

Edited by rooter (12/17/09 04:47 PM)

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Re: Must Read!! New Research Concludes: Cubes Unexposed to Light are 100 Xs more POTENT!! [Re: LeopardMan]
    #11669928 -

Quote:
Quote:
    PrimalSoup said:
    It's an electronic document, so, I don't know, email might work.
    -PS


So what? You can copy and paste it. It should be a pdf document.



God, is everybody irony impaired today???? :lol:

Peace
-PS

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Re: Must Read!! New Research Concludes: Cubes Unexposed to Light are 100 Xs more POTENT!! [Re: PrimalSoup]
    #11669978 -

They mentioned adding a "casing soil" and 10 days later introduced it to fruiting conditions. Why don't I see a casing layer in the pics?


--------------------
Everything posted by the user dancefloordale, aside from what is written in this here clause is completely and utterly fictitious, despite any information read (or seen), above (or below) that might lead you to believe otherwise.

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Re: Must Read!! New Research Concludes: Cubes Unexposed to Light are 100 Xs more POTENT!! [Re: Cloneufc]
    #11670016 -

I am very skeptical of this. If this were true I would expect albinos to be much more potent than non.

I could be wrong. I will put this to the test very shortly.

The claim 100 X potency seems too hyperbolic to be true.


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:

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Re: Must Read!! New Research Concludes: Cubes Unexposed to Light are 100 Xs more POTENT!! [Re: rooter]
    #11670017 -

Thanks to growguru for getting the info...:thumbup:

Quote:
As further evidence how would you account for dim light being worse than bright and no light?  Doesn't make sense.


Sure, dim probably acts as a trigger for some effect that vanishes in bright light and is never activated in no light.  Some plants are incredibly sensitive to light of particular frequencies even when it's administered only for microseconds.  Fungi aren't plants, but share a lot of biology.

So far, sounds like what I expected from the abstract and earlier discussion.  If anybody who has it would PM it to me I'd be happy to take a look...but I was working out and missed the feeding frenzy. :wink:

Peace
-PS


--------------------

if you stand too close to the machine it'll start to eat you
Primal's simple tested teks and projects: :awesomenod: Wheat Prep 2.0  Acidic Tea Tek  Potency Project! 

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Re: Must Read!! New Research Concludes: Cubes Unexposed to Light are 100 Xs more POTENT!! [Re: PrimalSoup]
    #11670075 -

PrimalSoup said:
Quote:
Quote:
    PrimalSoup said:
    It's an electronic document, so, I don't know, email might work.
    -PS


So what? You can copy and paste it. It should be a pdf document.



God, is everybody irony impaired today???? :lol:

Peace
-PS


(Yeah, I am dumb and need smart people in order to understand such subtle irony :lol:). Even if this study is not as good as we all hoped, it tells us that light COULD be a negative factor during fruiting. That's a pretty revolutionary statement IMO. Correct me if I am wrong.


--------------------



You have to die a few times before you can really live.

-Charles Bukowski-

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Re: Must Read!! New Research Concludes: Cubes Unexposed to Light are 100 Xs more POTENT!! [Re: rooter]
    #11670089 -

rooter said:
koopa_troopa said:
I seriously doubt a professional mycologist would submit any testing of fungi based around a multispore crapshot...really??


If they didn't say it...
... they didn't do it. 
Period.



I got the impression these are not mycologists, but chem students, or maybe med students.
Who got permission to grow shrooms and then wrote a paper on their grow log.
If they had posted this here, we would all have been high-fiving and five-shrooming these guys on their first endeavour.

rooter said:
PS to Doc_T: Yes, their English is flawless, isn't it?  Hmm...
(Nah)


Kinda weird but not bad. Better than my Farsi, that's for sure.


--------------------
You make it all possible. Doesn't it feel good?

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Re: Must Read!! New Research Concludes: Cubes Unexposed to Light are 100 Xs more POTENT!! [Re: LeopardMan]
    #11670163 -

Quote:
LeopardMan
(Yeah, I am dumb and need smart people in order to understand such subtle irony :lol:). Even if this study is not as good as we all hoped, it tells us that light COULD be a negative factor during fruiting. That's a pretty revolutionary statement IMO. Correct me if I am wrong.


Well, there's a number of respected people here who have made the opposite claim; that light enhances potency.

So I think the burden of proof here is on the proponents of this hyperbolic study, which appears to be of questionable rigor and methods.


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:

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Re: Must Read!! New Research Concludes: Cubes Unexposed to Light are 100 Xs more POTENT!! [Re: anonjon]
    #11670196 -

anonjon said:
Quote:
LeopardMan
(Yeah, I am dumb and need smart people in order to understand such subtle irony :lol:). Even if this study is not as good as we all hoped, it tells us that light COULD be a negative factor during fruiting. That's a pretty revolutionary statement IMO. Correct me if I am wrong.


Well, there's a number of respected people here who have made the opposite claim; that light enhances potency.

So I think the burden of proof here is on the proponents of this hyperbolic study, which appears to be of questionable rigor and methods.


I know. That's why I wrote could be. However I think we should somehow test their hyperbolic statements. You cant say they are true but you cant say they arent.:shrug:


--------------------



You have to die a few times before you can really live.

-Charles Bukowski-

Edited by LeopardMan (12/17/09 05:24 PM)

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Re: Must Read!! New Research Concludes: Cubes Unexposed to Light are 100 Xs more POTENT!! [Re: PrimalSoup]
    #11670204 -

Rooter, I wouldnt be so sure about that. I am friends with several professional mycologist (I know.. Im cooler than the other side of the pillow) and that factor is perhaps the single most assumed variable of any in studies involving fungi.. I find it very hard to believe some scientist hit up ralphsters for spores when they work in a mycology department that would have hundreds of isolates stored in a bank. Its probably more likely that they simply did not mention it bc it was not a commercial strain, as commercial ones have names or numbers to "trademark" the original creators work, therefore must be mentioned. A mycologist would know better than that and it is more likely they forgot to list it. 

Also, they most likely had to do atleast 3 grows, which makes it even less likely they would go multispore.. Either that or they had expiremented with the testing parameters before and knew the that the exposure to sunlight would have such a markedly drastic drop in actives that it would not matter at all if it was cloned or not! If you shoot one brother in the head with a gun, you know the exact same will happen to the other brother if you pulled the gun on him.. they need not be twins or genetically identical to predict this, as the effect is so drastic it would overcome any genetic differences or anomalies


--------------------

Koopa OutOfThisWorld,SmilingDownOnOurs: BirsdEyeView,NeverNewLife,PastsFastAsCars

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Re: Must Read!! New Research Concludes: Cubes Unexposed to Light are 100 Xs more POTENT!! [Re: LeopardMan]
    #11670209 -

Quote:
Even if this study is not as good as we all hoped, it tells us that light COULD be a negative factor during fruiting. That's a pretty revolutionary statement IMO. Correct me if I am wrong.


Nah, I'm with you.  If there's a real effect (I haven't read the study yet) that improves actives yield for the overall flush (not just getting a few puny fruits with greater potency, on account of the mycelium has to put that stuff somewhere) it can be manipulated creatively.  Different light durations during fruiting, different frequencies of light, this could all be done easily with computer control.  On/off stages can be milliseconds or less...

Light is important in certain ways, we know that already.  What can be done with it beyond it, that's what I'd like to know.

[this is an irony-free post - no iron has been y'd in the making of this post]

Peace
-PS


--------------------

if you stand too close to the machine it'll start to eat you
Primal's simple tested teks and projects: :awesomenod: Wheat Prep 2.0  Acidic Tea Tek  Potency Project! 

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Re: Must Read!! New Research Concludes: Cubes Unexposed to Light are 100 Xs more POTENT!! [Re: LeopardMan]
    #11670212 -

Quote:
I know. That's why I wrote could be. However I think we should somehow test their hyperbolic statements. You cant say they are true but you cant say they arent.:shrug:


Sure I can. Then I can refer to the shroomery cultivation forum for evidence that the opposite is true.


--------------------
The above post is fictional, hypothetical, or downright nonsensical.


:moon:  :moon:  :moon:  :moon:    :moon:

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Re: Must Read!! New Research Concludes: Cubes Unexposed to Light are 100 Xs more POTENT!! [Re: anonjon]
    #11670231 -

anonjon said:
Quote:
I know. That's why I wrote could be. However I think we should somehow test their hyperbolic statements. You cant say they are true but you cant say they arent.:shrug:


Sure I can. Then I can refer to the shroomery cultivation forum for evidence that the opposite is true.


:headbang3:
:scaryshroom::thumbup:


--------------------
:takingnotes:A M U:helpdesk:
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Re: Must Read!! New Research Concludes: Cubes Unexposed to Light are 100 Xs more POTENT!! [Re: anonjon]
    #11670244 -

anonjon said:
Quote:
I know. That's why I wrote could be. However I think we should somehow test their hyperbolic statements. You cant say they are true but you cant say they arent.:shrug:


Sure I can. Then I can refer to the shroomery cultivation forum for evidence that the opposite is true.


Ipse dixit? I like facts. And to have facts you need experiments.


--------------------



You have to die a few times before you can really live.

-Charles Bukowski-

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Re: Must Read!! New Research Concludes: Cubes Unexposed to Light are 100 Xs more POTENT!! [Re: koopa_troopa]
    #11670251 -

lol.. stuck in there for this many pages cant let it go down without some explanation.. atleast until I see it


--------------------

Koopa OutOfThisWorld,SmilingDownOnOurs: BirsdEyeView,NeverNewLife,PastsFastAsCars

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Re: Must Read!! New Research Concludes: Cubes Unexposed to Light are 100 Xs more POTENT!! [Re: LeopardMan]
    #11670325 -

I've read the study and looked at the pictures.  It's obvious these are noobs on their first grow, regardless of the fact they have access to a GC. I would bet money they're using multispore inoculation by the looks of the pictures.  I would also bet money they don't know how to adjust pinning triggers, by the looks of the crappy harvests they got.  Nor do they know what a casing layer is.  They also didn't quantify the light source or frequency used, only making high-schoolish references to dim, medium, bright, etc.

In addition, the pictures of fruits grown in poor light conditions also show signs of insufficient fresh air exchange, which could be skewing any results obtained.  It seems in their vigor to block light, they also blocked fresh air.  Based on the pictures, they only had a few fruits to work with, and as everyone here has found out by experience, there can be an exponential difference in potency between individual mushrooms on the same flush.  It takes a LOT of mushrooms to establish any sort of factual data.

Since these results contradict the experience of thousands of members here over the last decade and earlier, I'm going to dismiss this as a fluke.  How many times a week do we get noobs here saying things such as "this-or-that strain are fast colonizers, slow colonizers, more potent, less potent, more contam resistant, etc"., all based on one or two grows, when they haven't even learned the variables yet.

Furthermore, chemistry geeks are going to have a field day with their extraction technique, which involves heating the dried fruits, completely fucking up the psilocin which is what they were trying to measure.  They didn't even measure psilocybin, the main ingredient.
RR


--------------------
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"I've never had a failed experiment.  I've only discovered 10,000 methods which do not work."
Thomas Edison

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Re: Must Read!! New Research Concludes: Cubes Unexposed to Light are 100 Xs more POTENT!! [Re: RogerRabbit]
    #11670360 -

Well put RR, you voiced many concerns I had when reading over it.

:ilold: at the pictures they used.


--------------------
Everything posted by the user dancefloordale, aside from what is written in this here clause is completely and utterly fictitious, despite any information read (or seen), above (or below) that might lead you to believe otherwise.

Hydra Tek - A detailed guide, for newest to the most skilled cultivators.

HCA

Bulk growing made easy-discussion
Bulk Growing Made Easy

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Re: Must Read!! New Research Concludes: Cubes Unexposed to Light are 100 Xs more POTENT!! [Re: koopa_troopa]
    #11670385 -

In all honesty if it was multispore and the results came out that conclusive, even though there was such a diversified genetic makeup, with most genetic variants lying at either side of the average mean and enough grows (presuming 3) with each grow having hundreds of fruits, than the results would be just as conclusive as an isolate, if not more. For these reasons:

1) Using a random isolated substrain of P cubensis which has been isolated in no regard or chosen for any characteristics that relate to light sensitivity, then there is a larger than normal chance that the strain would not perform "realistically" to the results since you may inadvertantly use an isolate that is either more sensitive than the average cube to light degredation, or less sensitive. Either way you are not getting an indication of the natural population parameters (ie its natural or "average" reaction"

2) When statistically testing a random variable such as lights affect on alkaloid degredation and no prior experience with the testing. It would actually be more conducive and representative of Cubensis and a species as a whole to use multispore in sevaral large grows. This will give your random (random not isolate, very important) sample group (cubensis mushrooms grown in the test) a greater and random (very important) array of genetic variabilities, which given enough sample test statistics (number of mushrooms used in the extraction) will average out in the overall picture and be a true representative of the POPULATION (Cubes as a whole) not just the SAMPLE(testing only substrain).

I really didnt think about this until now but it is the best way to do these things given a random test variable and a large enough random sample. I hope atleast a few people get what im getting at lol..  it is actually correct scientifically and statistically.. LOL I would know I took my final senior bio stat final today muhuhahaha!! Let me know what you guys think about this hypothesis.. hope some of you are versed in stat!

NOW damzit someone send me that study!


--------------------

Koopa OutOfThisWorld,SmilingDownOnOurs: BirsdEyeView,NeverNewLife,PastsFastAsCars

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Re: Must Read!! New Research Concludes: Cubes Unexposed to Light are 100 Xs more POTENT!! [Re: koopa_troopa]
    #11670407 -

I think I read that they only did 2 grows. From looking at their pictures, I doubt they got a hundred fruits total, all experiments included.


--------------------
Everything posted by the user dancefloordale, aside from what is written in this here clause is completely and utterly fictitious, despite any information read (or seen), above (or below) that might lead you to believe otherwise.

Hydra Tek - A detailed guide, for newest to the most skilled cultivators.

HCA

Bulk growing made easy-discussion
Bulk Growing Made Easy

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Re: Must Read!! New Research Concludes: Cubes Unexposed to Light are 100 Xs more POTENT!! [Re: 13shrooms]
    #11670416 -

13shrooms said:
anonjon said:
Quote:
I know. That's why I wrote could be. However I think we should somehow test their hyperbolic statements. You cant say they are true but you cant say they arent.:shrug:


Sure I can. Then I can refer to the shroomery cultivation forum for evidence that the opposite is true.


:headbang3:
:scaryshroom::thumbup:


:rockon:
:highfive:


--------------------
You make it all possible. Doesn't it feel good?

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