langten69 said:

How big are your containers?

Timetable from TC Stipe n Cap:

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Baba Yaga said:

This doesn't hurt anything but I think as well that 16 min is cutting it a bit too tight. I do 25-30 min for LC and agar and vent for 15min beforehand.


Main things that can cause sediment in your LC during sterilization:

LME - contains protein which coagulates/clumps together when heated. If you putting a lot of LME in your LC you will get a lot of sediment, using
less will produce less sediment. The colour of your broth indicates you are using quite a bit of LME. To get rid of the proteins you could pre-boil the
broth and filter it.

Water - Very hard water can cause sediment due to lime precipitating during sterilization. This is what happened to me and it took me a while to figure
it out. Distilled or RO-water are best to use but bottled water is also ok if you can't get the other types, the cleaner the better, tap water can be crap.

Any other additives....


What is the percentage of LME in your broth? Looks like you could reduce it.


Page 4000

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Yeah I did vent for 10 minutes beforehand, then added the weight to the valve, an d after the first decompression I started the timer.

I wonder if starting again from scratch with a less concentrated solution and proper sterilization. I can imagine if I send this again to Pc it will only look worse.

What I use is a Liquid Culture mix from a vendor. I am not exactly sure what is in the mix (I thought I had read on their website that it was LME but just double checked and it doesnt say anything about whats in the mix), but the instructions say to use 4gr for every 100ml of water.



I used distilled water.

I have heaps of the LC mix, I think I will just start again, less saturated, maybe 2.5 or 3gr per 100ml? And sterilize for 30 min.

Thanks guys

Quote:

@Stipe n Cap said

Now onto liquid culture:


I prefer to use low nutrient concentration in the broth (.1%-.2%) for maximum clarity, clarity will allow you to easily visually identify bacterial contamination by the presence of turbidity (cloudiness). Turbidity is directly correlated to the severity of contamination by bacteria:



By utilizing a greater nutrient concentration in your broth (greater than .5%) you will end up with discolored broth and sedimentation, both of these will make visual inspection for turbidity difficult if not impossible.

Broth made @ 1% concentration compared to broth @ .1% (some folks are running broth @ 4+% which looks something like swamp water )

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0_tonin said:

Im all down for clarity. But from 4% to 0.1% we are talking a HUGE difference. Clarity is great I get it, but can such low concentration of nutrient carry some down side? Like slower growth?

I just tossed the previous solutions and did some from scratch. Some at 1%, some at 1.5% and some at 2%.

Will send to PC in a bit, going to roll a doobie for while I wait in the garage.

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jonkerston said:

Hey. I'm trying to make my first wheat grain jars. In my current attempt, I've cooked the grains on very low simmer until it's tender (I could eat that), but a lot of grains burst - see pics. Actually, it seems like the grain itself that I've bought has some cherries that are broken.

Is that a deal breaker? Does it make sense to proceed with what I've just cooked? Should I find a better source?

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0_tonin said:

Im all down for clarity. But from 4% to 0.1% we are talking a HUGE difference. Clarity is great I get it, but can such low concentration of nutrient carry some down side? Like slower growth?

I just tossed the previous solutions and did some from scratch. Some at 1%, some at 1.5% and some at 2%.

Will send to PC in a bit, going to roll a doobie for while I wait in the garage.

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0,2% DME

pilzezuechter said:

thanks for the answers.

to get it to field capacity i have to use 2:2:1 then. even then not a drop is coming out but it’s almost coming out. should
be wet enough right?

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langten69 said:

Quote:

@Stipe n Cap said

Now onto liquid culture:


I prefer to use low nutrient concentration in the broth (.1%-.2%) for maximum clarity, clarity will allow you to easily visually identify bacterial contamination by the presence of turbidity (cloudiness). Turbidity is directly correlated to the severity of contamination by bacteria:



By utilizing a greater nutrient concentration in your broth (greater than .5%) you will end up with discolored broth and sedimentation, both of these will make visual inspection for turbidity difficult if not impossible.

Broth made @ 1% concentration compared to broth @ .1% (some folks are running broth @ 4+% which looks something like swamp water )

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So a 2.5g - 3g per 100ml is a 2.5%-3% concentration

Depending on what your goals are, you may want to scale that back to a 0.2% if you are looking for clarity

A lot of people on the forum prefer a very lean culture just to make contam easier to spot

I know my last batch of LC came out a yellowish color and it does make turbidity slighty harder to percieve

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PuffM4gicDr4gon said:

DME = Dry malt extract

It's been a long time since I've made a liquid culture but less then 0.5% and they look real clear and real nice. I don't like sediment in my LC because then you can't tell the difference between that and the cloudiness caused by bacteria

A lot of the ogs here who use LC treat it as a single use item, so having slightly less mycelium in the liquid doesn't really matter.

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Lo-Z said:

Is DME 'DARK' Malt extract?  This looks really good Kloakk. I think my thoughts are the same as 0_Tonin,, whats the trade off? slower growth?  I know at 1.6% (what i've been running) I can populate 300ml in roughly 1-2 weeks. the downside is the sediment.

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In my opinion there is no trade off. They usually fill out 80ml within 2 weeks. I free pour and do single use. Often that means making 40ml for grains or 80ml if I'm doing AIO bags. What doesn't get used in one session goes down the toilet

PuffM4gicDr4gon said:

A lot of the ogs here who use LC treat it as a single use item, so having slightly less mycelium in the liquid doesn't really matter.

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Kloakk said:

What doesn't get used in one session goes down the toilet

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