Kloakk said:

pinkpuddles said:

I just finished pouring my first ever plates! Do you guys ever skip some by accident? lmao

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All the time or just come up short on agar, nice plates

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Agar Wedge said:

X7x+ T2

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bloodssonnew said:

2of these 4 I'ma have to retry the other 2 I got clean transfers of

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bloodssonnew said:

Yep during transfer those 4 got the green. I most likely didn't flame good enough or was sloppy n had my hand over it or something xD

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dextr0 said:

New FFU and 1/9 plate I made.

Probably been 2.5-3 yr hiatus. Lost everything I’d previously been working on for years.

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Nice FFU and welcome back

pilzezuechter said:

hello legends!

those are my first transfers and first clone attempt
and the first ist a germ plate.

do you guys think there is anything usable in here? ervthing just looks like mold to me


thanks for all the all the help i’ve already received here. without all yous it wouldn’t be possible!

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It doesn't seem that all is mold, top plate is toast, and I don't like the trails in the condensation.



Haven't grown gyms yet so can't really tell if the mycelium looks right or not, try having a look here.

JMF and PE can be okay, but the condensation on the lid is making it hard to see unfortunately. If you set a cup of hot water on top of the plates they should clear up enough to take some pictures.

Hehe, I am probably one of few who are using DME for dry malt extract around here because I come from brewing. If I am correct and you are using LME short for light malt extract, we are in fact talking about the same thing. In the brewing world LME is short for liquid malt extract.

Blue is a go, Red is a no.





This are just my opinion when looking at your pictures, if you see something that looks clean, organized and uniform when you go to do transfers go for it.

Contaminations can germ fast or slow, but yes it's normal, if you keep getting contamination around the edges, it could be worth trying to not use the first piece of cling wrap. Just toss the first round of it before wrapping a plate, if that makes sense.

Molds are hard to deal with, so when you do get fruits, remember to clone and print so you always have a way back to a culture.

For the plate with obvious contamination, just take a tiny piece of what looks like the good stuff furthest away from the contaminations.

And you are absolutely correct, when you have more fruits then you know what to do with, all sterile work becomes easier, my sterile technique also got better when I stopped being nervous for fucking everything up, funny how that works

But you got this dude, and should you ever need any new genetics just hit me with a

sandbar said:

I had some petris in the fridge since 2021 and took em out in 2026, clean white growth, although very stiff, when used a stainless steel scissor, inside a soft SAB (yes not recommended blablabla but better than nothing), to scrape and put some mycelium onto another plate. A week later it seems to work (germinate) so it is promising. I cannot remember how I made that recipe but... what I'm doing now: for 125 mL into 2 bottles. Potato flour 6g, sugar 2g, agar 4g, 1g beer yeast (2 varieties),  115g water. When taken out from Pressure Cooker, after 15 mins, it was too slimey and difficult to pour. Will have to adjust.

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Great job reviving an old plate

I just want to add that boiling brewers yeast doesn't really add much. For the nutrients in yeast to become bioavalable for the mycelium it needs to be broken down by enzymes.

That can be achieved by autolysis, but that is pretty time consuming so I recommend just getting nutritional yeast or Fermaid O