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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: fiddle_head]
    #29634491 -

that is a high tier capability, stick to plain agar
antibacterial agar likely also detriments mycelial growth, youcant have just one or the other. they both need food to grow. its like dumping a shitload of cholrine in a pool and expectring the bigger fish to live because theyre "bigger fish". some antibacterial methods work, but "antibacterial agar" in mycology in a practical sense, doesnt exist.


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: fiddle_head]
    #29634495 -

I haven't had a jar with bacterial ropes in a long time. Over a year. Condensation without an apparent cause has been happening for a while.

I know ABA not something used in mycology, just never knew why.

So what is my best plan of action dealing with a bacteria that can travel through both plastic and SFD filters, is airborne and on every surface, has infected every culture and spore print, as well as every tool of mine many of which can't be heat sterilized?

I know chemicals aren't traditionally used to sterilize things in this hobby and I also understand the difference between sanitization vs sterilization. It's also seeming like a strong chemical wash of everything I want to keep is in order.

What chemical should I use? Most chemicals only sanitize, which probably isn't good enough in this case. I routinely work with harsh chemicals so I have training and PPE to work with nastier stuff.

What is my best chance at getting past this beyond "be clean"?


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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: PuffM4gicDr4gon]
    #29634501 -

no, dont chemical wash everything, just focus on possible mistakes youre making during your clean procedural, in the sab and elsewhere.. flame sterilization and avoiding micro-currents with careful, deft movements are paramount. im not saying taking a full body scrub before clean work isnt important, but i always did it with success and never stopped doing it. flame sterilization is the only way to guarantee truly sterile which is defined as technically 6 logarithms which is 99.999999, because saying anytinng is 100% sterile, is like saying, mass can indeed travel at the speed of light.
no it cant, but it can get SO CLOSE it is practically and effectively the speed of light. hence "effectively sterile"="sterile" a connotation everyone can get behind in this hobby. nothing is ever gonna be 100%. its about ocntrolling variables until youve reached the core issues and have set parameters defined for your work.


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Edited by fiddle_head (07/28/26 06:27 AM)

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: fiddle_head]
    #29634502 -

Take a full-body shower, make your transfer naked or in a fresh set of clean clothes. I shut off the HVAC, kill any fans, close the door, and move slowly once everything has settled. The whole point of a SAB is still air, so don't create air currents once you're ready to work.

I clean the inside of the SAB with a diluted Dawn/bleach solution, then let it dry completely before I start. After that, I don't wipe everything down with alcohol because you're just introducing another opportunity to drag contaminants across the surface. Clean it first, let the air settle for 10–15 minutes, then get to work.

I'm not trying to be all Patrick Bateman about it, but eliminating as many variables as possible makes troubleshooting a whole lot easier. Once you've controlled the obvious contamination vectors, the mistakes become much easier to identify.


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: fiddle_head]
    #29634505 -

Sorry if I'm confused.

It was my understanding that Psuedonomads aren't just your run of the mill bacteria. The traditional 'clean' methods for getting rid of normal bacteria do not work. This is why I'm looking for outside ideas on how to deal with this.

The easiest way for me to understand this would be to confirm each of my assumptions based on the information I have been given.

1. The bacteria can't be left behind using Water agar, josex poke, cabin sequesters, trenching, or any other traditional method

2. The bacteria is small enough to bypass my SFD filters so even if I somehow get a clean culture it will become infected during the grain stage

3. The bacteria is small enough to be air borne so even if I make it past the agar and grain stage it is likely that my tubs will be infected as well.

4. The bacteria doesnt present any obvious symptoms during the agar stage. It's only prevalent during the grain and fruiting stage

5. Any spore prints I either do take or have taken from infected tubs will also be infected. This means that without interrupting or at least disrupting the life cycle of this organism there is no 'escaping' it

If that is not the case, please let me know because to me it seems like the traditional advice of "be clean" doesn't seem to apply.


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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

Edited by PuffM4gicDr4gon (07/28/26 06:39 AM)

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: PuffM4gicDr4gon]
    #29634514 -

For the record, I fully intend to keep working on my process. Tightening things up. Ruling out any other variables.

It's just that if Nichrome is right, then there is a plateau that I will never surpass unless I somehow cure it.


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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: PuffM4gicDr4gon]
    #29634522 -

Once your media is fully colonized—whether it's grain or bulk substrate—the mycelium is much more competitive and resistant to bacterial invasion than it is during the early stages. Bacteria can still be present, but by then the mycelium has usually established enough of a foothold that it isn't nearly as vulnerable.

That's why contamination control is so important early on. Bacteria only needs an opportunity: nutrients, moisture, and time before the mycelium takes over. Your goal is to prevent it from gaining that initial foothold while the culture is at its weakest.

Spore prints are never sterile. They're expected to carry some level of contamination, and that's just part of working from spores. What *can* be sterile is the tip of your tool, your media, and your technique. Flame sterilize properly, avoid hovering over open plates and jars, don't stir up microcurrents in your SAB, and eliminate as many variables as you can. That's how you stack the odds in your favor.


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⁂ guides | grows | supreme air box | sporeaddict ⁂

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: fiddle_head] * 1
    #29634526 -

OK thanks for the advice.

I guess I just treat this like any other infection and keep doing what I'm doing to get past it.

Maybe my 4th year trying to get past this will be the lucky one.


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I've got skills, they don't know where to use me
I'm like the best dressed guy at the nude beach
Nice to meet you!

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: Munchauzen]
    #29634629 -

Hello I wanted some help figuring some potential contaminants out. About 2.5 months ago I spawned 2 jars of grain with multispore syringes. I know this is not the optimal practice, but nonetheless is the one I know which has worked sufficiently well for my needs.
The substrate looked like this:



at some point it started to look like this, with mushrooms with super fuzzy feet:


Since its very hot I had made the substrate extra wet and I kept the exchange of fresh air low to avoid misting, and I never misted. The surface conditions looked very good and it flushed. Though at some point, another layer of white fluff started covering the bulk substrate and its pins, as if another mycelium were taking over.
At first I thought of contamination, cobweb mold, or early Trichoderma but it never shifted colour and remained like this. No new pins. Eventually it looked like this, and I noticed some larvae, maybe fungus gnat(?), and some very thin "cobweb" like strings going from the mycelium to the tub walls, strong enough to support the larva slithering on them





So I discarded it, but I tested the nature of the fuzziness which by touching it felt very much like mycelium, but nonetheless it showed no color change towards blue upon bruising. I used hydrogen peroxide 12vol. (3.6%) and it seemed to dissolve a bit of the mycelium. This to me confirmed contamination, but I still am not sure and want to know your opinion.

Any way I did a second run, still with multispore syringe on millet, the cakes colonized beautifully despite the heat. I made 3 tubs, and this is 2 of them right now:




Keep in mind that right now the temperature here has fluctuated between 30 and 36°C, with some wild fluctuations in humidity, 30-65% but mostly around 55%. I noticed a few weird things, some yellow discoloration and some green discoloration, which I cannot immediatly refer to contamination.


Here Tub A with some "metabolites"



Tub B with a bit of yellowing


Tub B with a bit of green discoloration near the lower  edge, touching the tub's wall.

Any way I love you all for the care and dedication, I want your opinion on this case, may it be a carryover contamination from the previous tubs? If so how can I avoid that? I also thought that the heat stress might have induced some mycelium damage. I dont know.
Thank you again.


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: emiliato]
    #29634707 -

That is probably fine. What you are seeing is likely just mycelium brusing. That overlay is from the temp swings, leave it alone it is not contamination. You have a perfectly good tub with primordia, slightly baccy or not.


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: fiddle_head]
    #29634708 -

It can look like and mimic the start of trich in my experience.


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: fiddle_head]
    #29635164 -

Hello! Before scrape the spores in the glass and mix with a syringe with sterile water;
What would be the best way to sterilize a glass shot for making a syringe with a spore print?


Option A: Flame-sterilize the glass shot with a torch inside the SAB.

Option B: Pour boiling water into the glass shot, put the lid, and transport it to the SAB .

Option C: just wipe with iso alcohol.

Edited by HighTurttle (07/29/26 01:40 AM)

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: HighTurttle]
    #29635170 -

First of all, what are you trying to accomplish?

Sterilize the shot glass as you would all your other tools in the autoclave. Do not wipe it with ISO; that doesn't do much. I don't know how other people do it, but you can put it in a jar and open it in the SAB if you're that worried about it.

I would just scrape the spores with your inoculation loop or scalpel onto the agar. It's simpler and doesn't require you to think so much.


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: fiddle_head]
    #29635171 -

You want to make a spore syringe correct? I'm asking because depending on your end goal, there might be alot easier way to go about it.


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: fiddle_head]
    #29635210 -

Yes, I want to prepare a syringe with a print. I don't have an autoclave. I want to know which of the two mentioned options is the best , to sterelize the glass where i will scrape the spores and mix with water. I am doing pf tek  with this syringe.

Edited by HighTurttle (07/29/26 04:24 AM)

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: HighTurttle]
    #29635451 -

Without an autoclave, you're not sterilizing anything.

You can steam sanitize your PF cakes on the stove, but that's the extent of cleanliness you'll be able to achieve.


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"For the powerful freedom-takers who rob justice from us all, that's why I keep my pistol loaded, powder, cap, and ball..."
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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: KlarnEvil9] * 3
    #29635561 -

Friendly reminder: this thread is for **Anyone Can Ask, Only TCs Can Answer.** Let's keep it that way so it stays organized.

As for your question, none of the options you listed will reliably sterilize the shot glass. ISO sanitizes surfaces; it doesn't sterilize them, and pouring boiling water into a container doesn't guarantee sterilization either.

Since you're doing PF Tek and don't have a pressure cooker, the traditional approach is to steam-sterilize your PF jars in a covered pot for an extended period, which has a long history of working with BRF cakes. That's one of the few places where atmospheric steam is generally sufficient.

For the syringe itself, I'd avoid making one unless you actually need it. A spore print is cleaner and simpler to use by transferring directly to agar. If your only goal is PF jars and you don't have sterile equipment to prepare a syringe, you're adding unnecessary contamination opportunities.

If you do decide to make a syringe anyway, the best approach is to steam-sterilize the shot glass (covered with foil) along with your other tools, and use previously sterilized water. It won't be equivalent to pressure sterilization, but it's a better approach than flame, boiling water poured into the glass, or wiping it with ISO.


--------------------
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⁂ guides | grows | supreme air box | sporeaddict ⁂

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: fiddle_head]
    #29635574 -

Thank you. They have pinned beautifully.


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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: fiddle_head]
    #29635606 -

fiddle_head said:
If you do decide to make a syringe anyway, the best approach is to steam-sterilize the shot glass (covered with foil) along with your other tools, and use previously sterilized water. It won't be equivalent to pressure sterilization, but it's a better approach than flame, boiling water poured into the glass, or wiping it with ISO.


This is technically a question so hope im not breaking a thread rule but couldn't a tin foiled shot glass be sterilized in an oven?


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So anyway, uh...

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Re: Official Trusted Cultivator Q&A Thread - Anyone Can Ask, Only TCs Can Answer! [Re: So Anyway]
    #29635624 -

sure, but why do that when you can do one of the reliably tested methods listed above, besides killing three birds with one stone with steam on a stove.


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