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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon] * 1
    #29428477 -

Phaethon said:
If your plates had trichoderma, I'd expect to see sporulation on the plate at some point.


Maybe, but I transfer from most of my plates long before they're colonized, and also I know what Trichoderma looks like before it starts to sporulate. So I'd probably toss the plate before it had a chance to turn green.


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Re: Ask Quick Questions, Get Quick Answers [Re: Phaethon] * 2
    #29428489 -

Phaethon said:
If your plates had trichoderma, I'd expect to see sporulation on the plate at some point. What exactly do you mean by "mostly clean"?

I think it's more likely your spawn was a little bacterial and/or not fully colonized before sending to bulk. Bacterial spawn makes for weak mycelial growth, leaving room for competitors.

I've also had a whole batch of shoeboxes from multiple spawn jars (different cultures) that looked clean go green before fruiting. I started being more careful about cleaning out old tubs, and I stopped compressing the substrate quite so much - anaerobic substrate is another way to give competitors a foothold.


One time I had a killer looking jar, recovered quick from a shake, colonized really well, very clean looking.

Then I shook it a second time and it erupted in a cloud of green that wasnt there when I started the shake

Filter failure is also no one to rule out


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk] * 2
    #29428533 -

Agar Wedge said:
I have a tub of x7x+ that Im starting to think may be sporeless. Only one mushroom dropped spores yesterday. Though I plan on waiting another day or 2 to be certain




Besides cloning from the best cluster, which I will do for sure. is there still technically spores I can collect if I swab the gils?


Yes, for instance, Penis Envy doesn't drop spores but you can rub one out of it with a q-tip, just rub it in the gills.

Are those low-spore x7x+ a cross? The look lighter in color too than usual. Or just came out that way?

Neat!


--------------------
--How to grow POPPIES.
--GROWING SHROOMS, FROM SPORES TO TRIPS
--x7x+ variety history.

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Re: Ask Quick Questions, Get Quick Answers [Re: nektar61]
    #29428611 -

Hey Nektar, was hoping to see you!:cool::mushroom2: These were from a spore print in DV8DUG's x7x+ give away. I don't believe they are a cross. I narrowed the genetics down over 5 transfers, going for the most rhizo growth before they even hit grain. guessing it just came out this way.

I'll give the gils a swab then and try to get germination on agar. I've made a bunch of clone plates from the fruits in the bottom picture. There was also the one mushroom that did drop spores so I cloned and swabbed that as well.

Really hoping these can be replicated! They've been fun to grow:mushroom2:


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Island clean up
Pasteurizing Straw with Hydrated Lime
Swiss Cheese Fruiting Chamber

Edited by Agar Wedge (12/13/25 07:31 PM)

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids]
    #29428619 -

tryptkaloids said:
Phaethon said:
If your plates had trichoderma, I'd expect to see sporulation on the plate at some point. What exactly do you mean by "mostly clean"?

I think it's more likely your spawn was a little bacterial and/or not fully colonized before sending to bulk. Bacterial spawn makes for weak mycelial growth, leaving room for competitors.

I've also had a whole batch of shoeboxes from multiple spawn jars (different cultures) that looked clean go green before fruiting. I started being more careful about cleaning out old tubs, and I stopped compressing the substrate quite so much - anaerobic substrate is another way to give competitors a foothold.


One time I had a killer looking jar, recovered quick from a shake, colonized really well, very clean looking.

Then I shook it a second time and it erupted in a cloud of green that wasnt there when I started the shake

Filter failure is also no one to rule out


Definitely not.

But the truth is, we just don't know. It could have been introduced and gotten a foothold at any point in the process. After all, that cloud of green had to be a network of off-white first. Which could have been lurking somewhere in the interior of the jar since the very beginning, just got a foothold due to an invisible bacterial infection, etc. I think it's unlikely that we're eliminating ALL trich spores from our jars. My working hypothesis is that it's just not terribly aggressive until conditions are just right, and healthy colonizing mushroom myc can usually take out just-starting-to-germinate Trichoderma spores.

I've had a couple cultures that looked absolutely amazing up until the last stages of colonization or the first stages of spawning, at which point they'd turn green. Narrowing it down to specific cultures told me that it wasn't an issue with any one jar or substrate source. Seeing the green turn up inside colonizing jars told me that the trich was already in the jar one way or another. Crappy lid? Introduced during the transfer? Enmeshed in the myc on the plate and just chillin? I don't know. My best guess is still that they were shot-through with bacteria and unable to fend anything off. I resolved the issue by ditching those culture lines and growing other ones lol


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Re: Ask Quick Questions, Get Quick Answers [Re: B Traven] * 1
    #29428623 -

💯

Honestly a big reason i learned to appreciate spores lol


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: nektar61] * 1
    #29428626 -

nektar61 said:
Agar Wedge said:
I have a tub of x7x+ that Im starting to think may be sporeless. Only one mushroom dropped spores yesterday. Though I plan on waiting another day or 2 to be certain




Besides cloning from the best cluster, which I will do for sure. is there still technically spores I can collect if I swab the gils?


Yes, for instance, Penis Envy doesn't drop spores but you can rub one out of it with a q-tip, just rub it in the gills.

Are those low-spore x7x+ a cross? The look lighter in color too than usual. Or just came out that way?

Neat!


If it was actually sporeless than the answer is no lol, PE makes spores it's just not good at ejection, but you will see the gills go purple , beefs gt for example simply doesn't make spores


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Ask Quick Questions, Get Quick Answers [Re: B Traven] * 2
    #29428630 -

Loving the new avatar cron


--------------------
"Remember, kids, the difference between science and screwing around is writing it down" -adam savage
Flowchart for Recommended plan of action.
Learn the tried and true way to grow mushrooms
Use the Damn search engine
After you know what you're doing, take a break 
Pick a book, Make some chips!
Josex said:Don't take the site seriously bro, ain't worth it.
 

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Re: Ask Quick Questions, Get Quick Answers [Re: tryptkaloids] * 1
    #29428636 -

I was just thinking that. Very cool


--------------------
A Love Letter to New Growers
The 7 Edicts of Mushroom Cultivation as Decreed by Smellyhobbit

:kodama:


Anyone can get a canopy. But can you get what you want?

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Re: Ask Quick Questions, Get Quick Answers [Re: cronicr]
    #29428641 -

Ahhhh okay, I see. They can be anywhere from low spore to sporeless. But there is a distinction. Thanks for clearing that up for me guys! I've only grown classic cubes so the low to no spore thing isnt something I'm used to :hehehe::mushroom2:


--------------------





Island clean up
Pasteurizing Straw with Hydrated Lime
Swiss Cheese Fruiting Chamber

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Re: Ask Quick Questions, Get Quick Answers [Re: Agar Wedge] * 1
    #29428655 -

It's highly sought after trait for gourmet , on a hobby level for cubes it's just kinda neat 😂


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

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Re: Ask Quick Questions, Get Quick Answers [Re: cronicr]
    #29428662 -

True that, I've got a sporeless oyster culture on the way I'm going to try out soon. I've heard the taste is different, but need to find out for myself.


--------------------





Island clean up
Pasteurizing Straw with Hydrated Lime
Swiss Cheese Fruiting Chamber

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: cronicr] * 3
    #29428663 -

cronicr said:
It's highly sought after trait for gourmet , on a hobby level for cubes it's just kinda neat 😂


Stellar avatar there cron, but yo, let's not fool our selves, x7x+ sporeless or low spore whatever, is a fucking revelation, they are original a cumbucket bukkake type of shroom.


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29428664 -

Ooki g at glass cover slips for culture work and holy shit can that be expensive! Can I just throw plastic slide covers in the PC if I want to do cabin sequestering?


--------------------


Everything is better when it is done ON TOP OF A MOUNTAIN!

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Re: Ask Quick Questions, Get Quick Answers [Re: Sulfurshelfsean] * 2
    #29428670 -

Sulfurshelfsean said:
Ooki g at glass cover slips for culture work and holy shit can that be expensive! Can I just throw plastic slide covers in the PC if I want to do cabin sequestering?


Just use agar as a roof :rockon:


--------------------
“All Fungi are edible.

Some fungi are only edible once.”
― Terry Pratchett


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Re: Ask Quick Questions, Get Quick Answers [Re: Agar Wedge]
    #29428673 -

tryptkaloids said:
Loving the new avatar cron


Smellyhobbit said:
I was just thinking that. Very cool


Thanks gents
Agar Wedge said:
True that, I've got a sporeless oyster culture on the way I'm going to try out soon. I've heard the taste is different, but need to find out for myself.


Yeah oyster spores are the worst lol


--------------------
It doesn't matter what i think of you...all that matters is clean spawn
I'm tired do me a favor

I'M A MEMBER FIRST, TC WHEN NEEDED AND MOD WHEN I HAVE TO BE !!!

Extras: Filter Print Post Top
Re: Ask Quick Questions, Get Quick Answers [Re: Sulfurshelfsean] * 2
    #29428674 -

If you're stuck on glass then lookit Scemilope's creative solution:

Scemilope said:
I have some 25mmx5mm glass tiles that I've been using for cabin roofs.





--------------------
MUSHROOMS RULE

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Re: Ask Quick Questions, Get Quick Answers [Re: Kloakk]
    #29428676 -

Kloakk said:
Sulfurshelfsean said:
Ooki g at glass cover slips for culture work and holy shit can that be expensive! Can I just throw plastic slide covers in the PC if I want to do cabin sequestering?


Just use agar as a roof :rockon:


Was gonna try that, but saw some opinions regarding bacteria bring able to work it's way 9ast an sugar roof. I'll try both ways!


--------------------


Everything is better when it is done ON TOP OF A MOUNTAIN!

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Re: Ask Quick Questions, Get Quick Answers [Re: Dala] * 1
    #29428677 -

Dala said:
If you're stuck on glass then lookit Scemilope's creative solution:

Scemilope said:
I have some 25mmx5mm glass tiles that I've been using for cabin roofs.





Will look into that too thanks!


--------------------


Everything is better when it is done ON TOP OF A MOUNTAIN!

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Re: Ask Quick Questions, Get Quick Answers [Re: Sulfurshelfsean] * 1
    #29428690 -

@nektar61 - Good to see you!

@cron - Wild avatar! 🤩

@AW - send you a pm about King 🦪.

@dala - been using agar, but scem’s glass tiles look cool. Thanks for posing that.

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Shop: North Spore Bulk Substrate   MagicBag.co All-In-One Bags That Don't Suck   Mushroom-Hut Substrate Bags   Sporeworks.EU Spores for European Microscopy   Original Sensible Seeds Bulk Cannabis Seeds   Myyco.com Golden Teacher Liquid Culture For Sale

Mushrooms, Mycology and Psychedelics > Mushroom Cultivation
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